EPS mechanisms in aggregation and dispersion of oil: roller tank experiment using Skeletonema CCMP strain #776

Aggregation and Degradation of Dispersants and Oil by Microbial Exopolymers (ADDOMEx)

DOI:
10.7266/N73N21SC
 
UDI:
R4.x263.188:0003
Last Update:
Feb 05 2018 23:44 UTC
 
Dataset Author(s):
Uta Passow
Point of Contact:
Passow, Uta
University of California Santa Barbara / Marine Science Institute
Bldg 520 Rm 4002 Fl 4L
Santa Barbara, California  93106
USA
uta.passow@lifesci.ucsb.edu
Funding Source:
RFP-IV
 
Extent Description:
Dataset contains laboratory measurements of WAF aggregates, no field sampling involved.

Identified Submitted In-Review Available
3 3 3 3

Suggested Citation:

Uta Passow. 2018. EPS mechanisms in aggregation and dispersion of oil: roller tank experiment using Skeletonema CCMP strain #776. Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N73N21SC

Abstract:

This is an experiment of a series investigating the mechanisms driving the incorporation of oil into diatom aggregates using a water accommodated fraction (WAF) of oil. The diatom Skeletonema CCMP strain #776 was used, and WAF was prepared from Macondo oil (Deepwater Horizon oil spill in the Gulf of Mexico April 2010) and from Refugio oil (pipeline spill off California May 2015). Roller tank experiments mimic in situ conditions of the ocean where in that continuous sinking of particles is possible (infinity water column). Once aggregates > 1mm (operationally defined) have formed, they are harvested separately from the surrounding seawater, which contains non-aggregates cells or small aggregates. Both fractions are analyzed separately, so that the fraction of material incorporated within aggregates may be calculated. Samples were analyzed for cell abundance, particulate organic carbon (POC) and nitrogen (PON), the isotopes 13C and 15N, estimated oil equivalent (EOE), transparent exopolymer particles (TEP) and Coomassie stained particles (CSP). Sinking velocity of aggregates was determined.

Purpose:

Experiment with 3 treatments (Control and ADDOMEx-WAF and Refugio-WAF) and two-time points using Skeletonema CCMP 776 to assess the effect of oil on marine snow formation and the incorporation of oil in marine snow. Marine snow and surrounding seawater (SSW) sampled separately.

Theme Keywords:

marine snow, marine oil snow, oil, particulate organic carbon, POC, particulate organic nitrogen, PON, Carbon-13, Nitrogen-15, estimated oil equivalent, EOE, transparent exopolymer particles, TEP, Coomassie stained particles, Macondo oil, Skeletonema

File Format:

csv

Filename:

003.zip (1.75 KB)

Dataset Downloads:

2

EPS mechanisms in aggregation and dispersion of oil: roller tank experiment using Skeletonema CCMP strain #776



Identification Information
Distribution Information
Metadata Maintenance Information

Metadata: 
  File identifier: 
      R4.x263.188-0003-metadata.xml
  Language: 
      eng; USA
  Character set: 
    Character set code: 
      utf8
  Hierarchy level: 
    Scope code: 
      dataset
  Metadata author: 
    Responsible party: 
      Individual name: 
          Kathleen (Kathy) Schwehr
      Organisation name: 
          Texas A&M University at Galveston / Marine Sciences Department
      Position name: 
          Associate Research Scientist
      Contact info: 
        Contact: 
          Phone: 
            Telephone: 
              Voice: 
                  4097404452
              Facsimile: 
          Address: 
            Address: 
              Delivery point: 
                  P.O. Box 1675
              City: 
                  Galveston
              Administrative area: 
                  Texas
              Postal code: 
                  77553
              Country: 
                  USA
              Electronic mail address: 
                  schwehrk@tamug.edu
      Role: 
        Role code: 
          pointOfContact
  Date stamp: 
      2019-02-12T23:09:09+00:00
  Metadata standard name: 
      ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
  Metadata standard version: 
      ISO 19115-2:2009(E)
  Dataset URI: 
      https://data.gulfresearchinitiative.org/metadata/R4.x263.188:0003
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Identification info: Data identification: Citation: Citation: Title: EPS mechanisms in aggregation and dispersion of oil: roller tank experiment using Skeletonema CCMP strain #776 Alternate title: Date: Date: Date: 2018-03-27 Date type: Date type code: publication Identifier: Identifier: Code: Anchor: xlink: https://dx.doi.org/10.7266/N73N21SC title: DOI doi:10.7266/N73N21SC Abstract: This is an experiment of a series investigating the mechanisms driving the incorporation of oil into diatom aggregates using a water accommodated fraction (WAF) of oil. The diatom Skeletonema CCMP strain #776 was used, and WAF was prepared from Macondo oil (Deepwater Horizon oil spill in the Gulf of Mexico April 2010) and from Refugio oil (pipeline spill off California May 2015). Roller tank experiments mimic in situ conditions of the ocean where in that continuous sinking of particles is possible (infinity water column). Once aggregates > 1mm (operationally defined) have formed, they are harvested separately from the surrounding seawater, which contains non-aggregates cells or small aggregates. Both fractions are analyzed separately, so that the fraction of material incorporated within aggregates may be calculated. Samples were analyzed for cell abundance, particulate organic carbon (POC) and nitrogen (PON), the isotopes 13C and 15N, estimated oil equivalent (EOE), transparent exopolymer particles (TEP) and Coomassie stained particles (CSP). Sinking velocity of aggregates was determined. Purpose: Experiment with 3 treatments (Control and ADDOMEx-WAF and Refugio-WAF) and two-time points using Skeletonema CCMP 776 to assess the effect of oil on marine snow formation and the incorporation of oil in marine snow. Marine snow and surrounding seawater (SSW) sampled separately. Status: Progress code: completed Point of contact: Responsible party: Individual name: Uta Passow Organisation name: University of California Santa Barbara / Marine Science Institute Position name: Researcher Contact info: Contact: Phone: Telephone: Voice: 8058932363 Facsimile: Address: Address: Delivery point: Bldg 520 Rm 4002 Fl 4L City: Santa Barbara Administrative area: California Postal code: 93106 Country: USA Electronic mail address: uta.passow@lifesci.ucsb.edu Role: Role code: pointOfContact Descriptive keywords: Keywords: Keyword: marine snow Keyword: marine oil snow Keyword: oil Keyword: particulate organic carbon Keyword: POC Keyword: particulate organic nitrogen Keyword: PON Keyword: Carbon-13 Keyword: Nitrogen-15 Keyword: estimated oil equivalent Keyword: EOE Keyword: transparent exopolymer particles Keyword: TEP Keyword: Coomassie stained particles Keyword: Macondo oil Keyword: Skeletonema Type: Keyword type code: theme Descriptive keywords: Keywords: Keyword: inapplicable Type: Keyword type code: place Resource constraints: title: Cite As Constraints: Use limitation: Uta Passow. 2018. EPS mechanisms in aggregation and dispersion of oil: roller tank experiment using Skeletonema CCMP strain #776. Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N73N21SC Resource constraints: title: CC0 License Legal constraints: Use constraints: Restriction code: licenceUnrestricted Other constraints: This information is released under the Creative Commons license - No Rights Reserved - CC0 1.0 Universal (https://creativecommons.org/publicdomain/zero/1.0/). The person who associated a work with this deed has dedicated the work to the public domain by waiving all of his or her rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law. You can copy, modify, distribute and perform the work, even for commercial purposes, all without asking permission. Resource constraints: title: Liability and Warranty Legal constraints: Other constraints: All materials on this website are made available to GRIIDC and in turn to you "as-is." Content may only be submitted by an individual who represents and warrants that s/he has sufficient rights to be able to make the content available under a CC0 waiver. There is no warranty (expressed or implied) to these materials, their title, accuracy, non-infringement of third party rights, or fitness for any particular purpose, including the performance or results you may obtain from their use. Use these materials at your own risk. Under no circumstances shall GRIIDC be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use or the inability to use either this website or the materials available via this website. If you are dissatisfied with any website feature, content, or terms of use, your sole and exclusive remedy is to discontinue use. Aggregation Info: AggregateInformation: Aggregate Data Set Name: title: Related Publication Citation Citation: Title: Passow, U., Sweet, J., Francis, S., Xu, C., Dissanayake, A., Lin, Y., … Quigg, A. (2019). Incorporation of oil into diatom aggregates. Marine Ecology Progress Series, 612, 65–86. doi:10.3354/meps12881 Date: inapplicable Aggregate Data Set Identifier: title: Related Publication DOI Identifier: Code: Anchor: xlink: https://dx.doi.org/10.3354/meps12881 title: DOI doi:10.3354/meps12881 Association Type: Association type code: crossReference Language: eng; USA Topic category: Topic category code: oceans Topic category: Topic category code: biota Topic category: Topic category code: environment Extent: Extent: Description: Dataset contains laboratory measurements of WAF aggregates, no field sampling involved. Supplemental Information: GRIIDC-WAF-Agg Sinking Velocity R4x263-188-0003.csv: Experiment: exp. Treatment: WAFMac, Control, or WAFRef Water accommodated fraction of Macondo oil = WAFMAC Water accommodated fraction of Refugio oil – WAFRef Control = no oil Aggregate ID number: Agg # Equivalent spherical diameter (mm): ESD Sinking velocity (m/d) = w GRIIDC-WAF-Agg Summary R4x263-188-0003.csv: Experiment: Exp Tank number = tank # Sampling date (MM/DD/YYYY) Sampling day: number of experiment days Treatment: Control, Macondo, or Refugio Replicate number Type of sample (Slurry or SSW) Slurry = the fraction of aggregates > 1 mm with some surrounding seawater SSW = surrounding seawater fraction Tank Vol/Slurry Volume (mL): Maximum tank volume (5133 mL)/ Slurry Volume Avg POC (ug/tank): Average particulate organic carbon Std POC (ug/tank): Standard deviation particulate organic carbon Avg PON (ug/tank): Average particulate organic nitrogen Std PON (ug/tank): Standard deviation particulate organic nitrogen Avg d13CNorm: Average delta Carbon-13 (unitless) standard expressed as per mille:(δ13C = (Rsample/Rstandard -1) X 1000, where R = 13C/12C) Stdev d13CNorm: Standard deviation delta Carbon-13 (unitless) Avg d15NNorm: Average delta Nitrogen-15 (unitless) Stdev d15NNorm: Standard deviation delta Nitrogen-15 (unitless) Avg EOE corrected (ug/tank): Average estimate oil equivalent Std EOE corrected (ug/tank): Standard deviation estimate oil equivalent Avg TEP (ugGXeq/Tank): Average transparent exopolymer particles Std TEP (ugGXeq/Tank): Standard deviation transparent exopolymer particles Gum Xanthan equivalent = GXeq Avg CSP (BSAeq/Tank): Average Coomassie blue stainable particles Std CSP (BSAeq/Tank): Standard deviation Coomassie blue stainable particles BSAeq: Bovine serum albumin equivalent Avg cell numbers per tank: Average number of cells per tank Coomassie Blue stainable particles = CSP ND = no data NA = not applicable NV = not valid |Replicate acrylic roller tanks were filled bubble free. Experiments were incubated in the dark, at 13˚C at an rpm of 1.9-3.2 Aggregate appearance and numbers were monitored during the experiments. When sufficient aggregates had formed, all treatments were harvested. Aggregates > 1mm (visually discernable) were manually collected and analyzed. After all aggregates > 1 mm were removed the remainder of the material, termed surrounding seawater (SSW), was subsampled. Both fractions were analyzed for POC/PON, cell abundance, TEP, CSP. Relative oil concentration was estimated from fluorescence using a Trilogy Turner Fluorometer with the crude oil module 7200-63, which measures at excitation wavelength of 365 nm and emission wavelength of 410-600nm. Duplicate filters (GF/F) prepared for POC/PON analysis were measured in a CEC44OHA elemental analyzer (Control equipment). Diatom cells were counted (Olympus CX41) using a hemocytometer; at least 6 subsamples and 200 cells each were counted per sample. Counts were at times only conducted on 1 of the two replicate treatments. TEP and CSP concentrations were determined in triplicate each using the respective colorimetric methods and are expressed in Gum Xanthan equivalents (GXeq.) and Bovine serum albumin equivalent (BSA eq.) respectively. All biomass results (cell abundance, POC, TEP concentration) are normalized per tank to allow budgets and make aggregate and SSW fractions directly comparable. Make WAF directly before use. Make 3L filtered seawater (0.2 µm filter), and pasteurize it for at least 2 hours at 65 degrees C. Add into 4+ L glass bottle with bottom spigot and glass stopper. Leave 20-25% headspace. Add a sterile stir bar to the bottle and add 3 mL (3000 µL) oil. Immediately cap, shake. Place on a stir table at room temperature for about 24 hours at a fairly high stirring speed (not enough to generate a vortex, but enough to keep things homogenized). Keep dark during mixing (alu foil or brown glass). Let the bottles stand for a couple hours. Then, harvest discarding the upper oily layer. Sinking velocity measurements were conducted using the column/ setcol method and the non-destructive orbit method of calculating sinking velocities based on tank rotation speed and the orbital path of aggregates within the turning tanks. Ploug, H., Terbrüggen, A., Kaufmann, A., Wolf-Gladrow, D. and Passow, U. (2010) A novel method to measure particle sinking velocity in vitro, and its comparison to three other in vitro methods. Limnology and Oceanography: Methods 8, 386-393.||micrometer to decimeter, days||
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Distribution info: Distribution: Distributor: Distributor: Distributor contact: Responsible party: Organisation name: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC) Contact info: Contact: Phone: Telephone: Voice: 3618253604 Address: Address: Delivery point: 6300 Ocean Drive City: Corpus Christi Administrative area: TX Postal code: 78412 Country: USA Electronic mail address: griidc@gomri.org Online Resource: Online Resource: Linkage: URL: https://data.gulfresearchinitiative.org Role: Role code: distributor Distributor format: Format: Name: csv Version: inapplicable File decompression technique: zip Distributor transfer options: Digital transfer options: Transfer size: 0.0018 Online: Online Resource: Linkage: URL: https://data.gulfresearchinitiative.org/data/R4.x263.188:0003 Protocol: https Name: Data Landing Page Description: GRIIDC dataset landing page Function: Online function code: information
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Metadata maintenance: Maintenance information: Maintenance and update frequency: unknown Maintenance note: This ISO metadata record was automatically generated from information provided to GRIIDC for dataset: R4.x263.188:0003 on 2020-09-24T10:46:10-05:00
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