EPS mechanisms in aggregation and dispersion of oil: roller tank experiment using Skeletonema, POC-PON-EOE-TEP-CSP

Aggregation and Degradation of Dispersants and Oil by Microbial Exopolymers (ADDOMEx)

DOI:
10.7266/N7ZW1JBJ
 
UDI:
R4.x263.188:0002
Last Update:
Feb 05 2018 23:51 UTC
 
Dataset Author(s):
Uta Passow
Point of Contact:
Passow, Uta
University of California Santa Barbara / Marine Science Institute
Bldg 520 Rm 4002 Fl 4L
Santa Barbara, California  93106
USA
uta.passow@lifesci.ucsb.edu
Funding Source:
RFP-IV
 
Extent Description:
Dataset contains laboratory measurements of WAF aggregation, no field sampling involved.

Identified Submitted In-Review Available
3 3 3 3

Suggested Citation:

Uta Passow. 2018. EPS mechanisms in aggregation and dispersion of oil: roller tank experiment using Skeletonema, POC-PON-EOE-TEP-CSP. Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7ZW1JBJ

Abstract:

This is an experiment of a series investigating the mechanisms driving the incorporation of oil into diatom aggregates using a water accommodated fraction (WAF) of oil. The diatom Skeletonema CCMP strain #775 was used to assess the effect of oil on marine snow formation and the incorporation of oil in marine snow. A control and a WAF was prepared from Macondo oil (Deepwater Horizon oil spill in the Gulf of Mexico, April 2010). Roller tank experiments mimic in situ conditions of the ocean in that continuous sinking of particles is possible (infinity water column). Once aggregates > 1mm (operationally defined) formed, they were harvested separately from the surrounding seawater, which contains non-aggregates cells or small aggregates. Both fractions were analyzed separately, so that the fraction of material incorporated within aggregates may be calculated. Samples were analyzed for particulate organic carbon (POC) and nitrogen (PON), the isotopes Carbon-13 and Nitrogen-15, estimated oil equivalent (EOE), transparent exopolymer particles (TEP), and coomassie-stained particles (CSP).

Purpose:

Small preliminary test experiment with 2 treatments (Control and WAF) using Skeletonema CCMP 775 to assess the effect of oil on marine snow formation and the incorporation of oil in marine snow. Marine snow and surrounding seawater (SSW) sampled separately.

Theme Keywords:

marine snow, marine oil snow, oil, particulate organic carbon, POC, particulate organic nitrogen, PON, Carbon-13, Nitrogen-15, EOE, transparent exopolymer particles, TEP, water accommodated fraction, WAF, coomssie stained particles

File Format:

csv

Filename:

GRIIDC-WAF-Agg-Summary R4x263-188-0002.csv (858 B)

Dataset Downloads:

4

EPS mechanisms in aggregation and dispersion of oil: roller tank experiment using Skeletonema, POC-PON-EOE-TEP-CSP



Identification Information
Distribution Information
Metadata Maintenance Information

Metadata: 
  File identifier: 
      R4.x263.188-0002-metadata.xml
  Language: 
      eng; USA
  Character set: 
    Character set code: 
      utf8
  Hierarchy level: 
    Scope code: 
      dataset
  Metadata author: 
    Responsible party: 
      Individual name: 
          Kathleen (Kathy) Schwehr
      Organisation name: 
          Texas A&M University at Galveston / Marine Sciences Department
      Position name: 
          Associate Research Scientist
      Contact info: 
        Contact: 
          Phone: 
            Telephone: 
              Voice: 
                  4097404452
              Facsimile: 
          Address: 
            Address: 
              Delivery point: 
                  P.O. Box 1675
              City: 
                  Galveston
              Administrative area: 
                  Texas
              Postal code: 
                  77553
              Country: 
                  USA
              Electronic mail address: 
                  schwehrk@tamug.edu
      Role: 
        Role code: 
          pointOfContact
  Date stamp: 
      2019-02-12T23:05:42+00:00
  Metadata standard name: 
      ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
  Metadata standard version: 
      ISO 19115-2:2009(E)
  Dataset URI: 
      https://data.gulfresearchinitiative.org/metadata/R4.x263.188:0002
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Identification info: Data identification: Citation: Citation: Title: EPS mechanisms in aggregation and dispersion of oil: roller tank experiment using Skeletonema, POC-PON-EOE-TEP-CSP Alternate title: Date: Date: Date: 2018-03-23 Date type: Date type code: publication Identifier: Identifier: Code: Anchor: xlink: https://dx.doi.org/10.7266/N7ZW1JBJ title: DOI doi:10.7266/N7ZW1JBJ Abstract: This is an experiment of a series investigating the mechanisms driving the incorporation of oil into diatom aggregates using a water accommodated fraction (WAF) of oil. The diatom Skeletonema CCMP strain #775 was used to assess the effect of oil on marine snow formation and the incorporation of oil in marine snow. A control and a WAF was prepared from Macondo oil (Deepwater Horizon oil spill in the Gulf of Mexico, April 2010). Roller tank experiments mimic in situ conditions of the ocean in that continuous sinking of particles is possible (infinity water column). Once aggregates > 1mm (operationally defined) formed, they were harvested separately from the surrounding seawater, which contains non-aggregates cells or small aggregates. Both fractions were analyzed separately, so that the fraction of material incorporated within aggregates may be calculated. Samples were analyzed for particulate organic carbon (POC) and nitrogen (PON), the isotopes Carbon-13 and Nitrogen-15, estimated oil equivalent (EOE), transparent exopolymer particles (TEP), and coomassie-stained particles (CSP). Purpose: Small preliminary test experiment with 2 treatments (Control and WAF) using Skeletonema CCMP 775 to assess the effect of oil on marine snow formation and the incorporation of oil in marine snow. Marine snow and surrounding seawater (SSW) sampled separately. Status: Progress code: completed Point of contact: Responsible party: Individual name: Uta Passow Organisation name: University of California Santa Barbara / Marine Science Institute Position name: Researcher Contact info: Contact: Phone: Telephone: Voice: 8058932363 Facsimile: Address: Address: Delivery point: Bldg 520 Rm 4002 Fl 4L City: Santa Barbara Administrative area: California Postal code: 93106 Country: USA Electronic mail address: uta.passow@lifesci.ucsb.edu Role: Role code: pointOfContact Descriptive keywords: Keywords: Keyword: marine snow Keyword: marine oil snow Keyword: oil Keyword: particulate organic carbon Keyword: POC Keyword: particulate organic nitrogen Keyword: PON Keyword: Carbon-13 Keyword: Nitrogen-15 Keyword: EOE Keyword: transparent exopolymer particles Keyword: TEP Keyword: water accommodated fraction Keyword: WAF Keyword: coomssie stained particles Type: Keyword type code: theme Descriptive keywords: Keywords: Keyword: inapplicable Type: Keyword type code: place Resource constraints: title: Cite As Constraints: Use limitation: Uta Passow. 2018. EPS mechanisms in aggregation and dispersion of oil: roller tank experiment using Skeletonema, POC-PON-EOE-TEP-CSP. Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7ZW1JBJ Resource constraints: title: CC0 License Legal constraints: Use constraints: Restriction code: licenceUnrestricted Other constraints: This information is released under the Creative Commons license - No Rights Reserved - CC0 1.0 Universal (https://creativecommons.org/publicdomain/zero/1.0/). The person who associated a work with this deed has dedicated the work to the public domain by waiving all of his or her rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law. You can copy, modify, distribute and perform the work, even for commercial purposes, all without asking permission. Resource constraints: title: Liability and Warranty Legal constraints: Other constraints: All materials on this website are made available to GRIIDC and in turn to you "as-is." Content may only be submitted by an individual who represents and warrants that s/he has sufficient rights to be able to make the content available under a CC0 waiver. There is no warranty (expressed or implied) to these materials, their title, accuracy, non-infringement of third party rights, or fitness for any particular purpose, including the performance or results you may obtain from their use. Use these materials at your own risk. Under no circumstances shall GRIIDC be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use or the inability to use either this website or the materials available via this website. If you are dissatisfied with any website feature, content, or terms of use, your sole and exclusive remedy is to discontinue use. Aggregation Info: AggregateInformation: Aggregate Data Set Name: title: Related Publication Citation Citation: Title: Passow, U., Sweet, J., Francis, S., Xu, C., Dissanayake, A., Lin, Y., … Quigg, A. (2019). Incorporation of oil into diatom aggregates. Marine Ecology Progress Series, 612, 65–86. doi:10.3354/meps12881 Date: inapplicable Aggregate Data Set Identifier: title: Related Publication DOI Identifier: Code: Anchor: xlink: https://dx.doi.org/10.3354/meps12881 title: DOI doi:10.3354/meps12881 Association Type: Association type code: crossReference Language: eng; USA Topic category: Topic category code: oceans Topic category: Topic category code: biota Topic category: Topic category code: environment Extent: Extent: Description: Dataset contains laboratory measurements of WAF aggregation, no field sampling involved. Supplemental Information: Experiment: exp. Tank number = tank # Sampling date (MM/DD/YYYY) Sampling day = number of experiment days Treatment (Control or Macondo) Replicate #: number of replicates Type of sample (slurry or SSW) Slurry = the fraction of aggregates > 1 mm with some surrounding seawater SSW = surrounding seawater fraction Tank volume/slurry volume (mL): Total tank volume (1160 mL)/ Slurry Volume Avg POC (ug/tank): Average Particulate Organic Carbon Std POC (ug/tank): Standard deviation Particulate Organic Carbon Avg PON (ug/tank): Average Particulate Organic Nitrogen Std PON (ug/tank): Standard deviation Particulate Organic Nitrogen Avg d13CNorm: Average delta13C (unitless). Relative value compared to a standard expressed as per mille:(δ13C = (Rsample/Rstandard -1) X 1000, where R = 13C/12C) Stdev d13CNorm: Standard deviation delta13C (unitless) Avg d15NNorm: Average delta15N (unitless) Stdev d15NNorm: Standard deviation delta15N (unitless) Avg EOE corrected (ug/tank): Average Estimate Oil Equivalent Std EOE corrected (ug/tank): Standard deviation Estimate Oil Equivalent Avg TEP (ugGXeq/Tank): Average transparent exopolymer particles Std TEP (ugGXeq/Tank): Average transparent exopolymer particles Gum Xanthan equivalent = GXeq Avg CSP (BSAeq/tank): Average Coomssie Blue stainable particles Std CSP (BSAeq/tank): Standard deviation Coomssie Blue stainable particles BSAeq=Bovine serum albumin equivalent ND = no data|Replicate acrylic roller tanks were filled bubble free. Experiments were incubated in the dark, at 22˚C at an rpm of 1.4 Aggregate appearance and numbers were monitored during the experiments. When sufficient aggregates had formed, all treatments were harvested. Aggregates > 1mm (visually discernable) were manually collected and analyzed. After all aggregates > 1 mm were removed the remainder of the material, termed surrounding seawater (SSW), was subsampled. Both fractions were analyzed for POC/PON, cell abundance, TEP, CSP. Relative oil concentration was estimated from fluorescence using a Trilogy Turner Fluorometer with the crude oil module 7200-63, which measures at excitation wavelength of 365 nm and emission wavelength of 410-600nm. Duplicate filters (GF/F) prepared for POC/PON analysis were measured in a CEC44OHA elemental analyzer (Control equipment). TEP concentrations were determined in triplicate using the colorimetric methods and are expressed in Gum Xanthan equivalents (GXeq. All biomass results (POC, TEP concentration) are normalized per tank to allow budgets and make aggregate and SSW fractions directly comparable. Made WAF directly before use. Made 3L filtered seawater (0.2 µm filter), and pasteurized it for at least 2 hours at 65 degrees C. Added into 4+ L glass bottle with bottom spigot and glass stopper. Left 20-25% headspace. Added a sterile stir bar to the bottle and add 3 mL (3000 µL) oil. Immediately cap and shake. Placed on a stir table at room temperature for about 24 hours at a fairly high stirring speed (not enough to generate a vortex, but enough to keep things homogenized). Kept dark during mixing (aluminum foil or brown glass). Let the bottles stand for a couple hours. Then, harvested discarding the upper oily layer.||micrometer to decimeter, days||
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Distribution info: Distribution: Distributor: Distributor: Distributor contact: Responsible party: Organisation name: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC) Contact info: Contact: Phone: Telephone: Voice: 3618253604 Address: Address: Delivery point: 6300 Ocean Drive City: Corpus Christi Administrative area: TX Postal code: 78412 Country: USA Electronic mail address: griidc@gomri.org Online Resource: Online Resource: Linkage: URL: https://data.gulfresearchinitiative.org Role: Role code: distributor Distributor format: Format: Name: csv Version: inapplicable File decompression technique: Distributor transfer options: Digital transfer options: Transfer size: 0.0009 Online: Online Resource: Linkage: URL: https://data.gulfresearchinitiative.org/data/R4.x263.188:0002 Protocol: https Name: Data Landing Page Description: GRIIDC dataset landing page Function: Online function code: information
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Metadata maintenance: Maintenance information: Maintenance and update frequency: unknown Maintenance note: This ISO metadata record was automatically generated from information provided to GRIIDC for dataset: R4.x263.188:0002 on 2020-09-24T10:40:46-05:00
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