Roller Tank Experiment for the component-specific Investigation of Oil Incorporation into Marine Oil Snow with a Phytoplankton Culture (Nodularia) (t = 24 hours)

Aggregation and Degradation of Dispersants and Oil by Microbial Exopolymers (ADDOMEx)

DOI:
10.7266/N7TX3CTM
 
UDI:
R4.x263.000:0035
Last Update:
Oct 26 2017 21:16 UTC
 
Dataset Author(s):
Uta Passow, Marisa Wirth, D. Schulz-Bull
Point of Contact:
Passow, Uta
University of California Santa Barbara / Marine Science Institute
Bldg 520 Rm 4002 Fl 4L
Santa Barbara, California  93106
USA
uta.passow@lifesci.ucsb.edu
Funding Source:
RFP-IV
 
Extent Description:
Dataset contains laboratory measurements of oil incorporation into marine snow, no field sampling involved.

Identified Submitted In-Review Available
3 3 3 3

Suggested Citation:

Uta Passow, Marisa Wirth, D. Schulz-Bull. 2017. Roller Tank Experiment for the component-specific Investigation of Oil Incorporation into Marine Oil Snow with a Phytoplankton Culture (Nodularia) (t = 24 hours). Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7TX3CTM

Abstract:

This roller tank experiment investigated the partitioning of different oil compounds (n-alkanes and polyaromatic hydrocarbons) between sinking marine oil snow, the unaggregated particles in the surrounding seawater and the dissolved water phase. Moreover, the effect of the dispersant Corexit on the occurring processes was investigated. For this experiment, a cyanobacterium culture (Nodularia) was used to form marine oil snow (MOS). The aim was to gain new insights into the oil incorporation mechanisms into marine snow.

Purpose:

Roller tank experiment to investigate the partitioning of oil compounds into marine snow made from a cyanobacteria.

Theme Keywords:

marine snow, aggregates, phytoplankton, cyanobacteria, oil partitioning, Corexit, Deepwater Horizon, PAH, n-alkanes, POC, DOC

File Format:

csv

Filename:

Exp2.csv (12.87 KB)

Dataset Downloads:

2

Roller Tank Experiment for the component-specific Investigation of Oil Incorporation into Marine Oil Snow with a Phytoplankton Culture (Nodularia) (t = 24 hours)



Identification Information
Distribution Information
Metadata Maintenance Information

Metadata: 
  File identifier: 
      R4.x263.000-0035-metadata.xml
  Language: 
      eng; USA
  Character set: 
    Character set code: 
      utf8
  Hierarchy level: 
    Scope code: 
      dataset
  Metadata author: 
    Responsible party: 
      Individual name: 
          Kathleen (Kathy) Schwehr
      Organisation name: 
          Texas A&M University at Galveston / Marine Sciences Department
      Position name: 
          Associate Research Scientist
      Contact info: 
        Contact: 
          Phone: 
            Telephone: 
              Voice: 
                  4097404452
              Facsimile: 
          Address: 
            Address: 
              Delivery point: 
                  P.O. Box 1675
              City: 
                  Galveston
              Administrative area: 
                  Texas
              Postal code: 
                  77553
              Country: 
                  USA
              Electronic mail address: 
                  schwehrk@tamug.edu
      Role: 
        Role code: 
          pointOfContact
  Date stamp: 
      2018-12-13T19:34:56+00:00
  Metadata standard name: 
      ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
  Metadata standard version: 
      ISO 19115-2:2009(E)
  Dataset URI: 
      https://data.gulfresearchinitiative.org/metadata/R4.x263.000:0035
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Identification info: Data identification: Citation: Citation: Title: Roller Tank Experiment for the component-specific Investigation of Oil Incorporation into Marine Oil Snow with a Phytoplankton Culture (Nodularia) (t = 24 hours) Alternate title: Component-specific Investigation of Oil Incorporation into Marine Oil Snow with a Phytoplankton Culture (t = 24 hours) Date: Date: Date: 2017-11-13 Date type: Date type code: publication Identifier: Identifier: Code: Anchor: xlink: https://dx.doi.org/10.7266/N7TX3CTM title: DOI doi:10.7266/N7TX3CTM Abstract: This roller tank experiment investigated the partitioning of different oil compounds (n-alkanes and polyaromatic hydrocarbons) between sinking marine oil snow, the unaggregated particles in the surrounding seawater and the dissolved water phase. Moreover, the effect of the dispersant Corexit on the occurring processes was investigated. For this experiment, a cyanobacterium culture (Nodularia) was used to form marine oil snow (MOS). The aim was to gain new insights into the oil incorporation mechanisms into marine snow. Purpose: Roller tank experiment to investigate the partitioning of oil compounds into marine snow made from a cyanobacteria. Status: Progress code: completed Point of contact: Responsible party: Individual name: Uta Passow Organisation name: University of California Santa Barbara / Marine Science Institute Position name: Researcher Contact info: Contact: Phone: Telephone: Voice: 8058932363 Facsimile: Address: Address: Delivery point: Bldg 520 Rm 4002 Fl 4L City: Santa Barbara Administrative area: California Postal code: 93106 Country: USA Electronic mail address: uta.passow@lifesci.ucsb.edu Role: Role code: pointOfContact Descriptive keywords: Keywords: Keyword: marine snow Keyword: aggregates Keyword: phytoplankton Keyword: cyanobacteria Keyword: oil partitioning Keyword: Corexit Keyword: Deepwater Horizon Keyword: PAH Keyword: n-alkanes Keyword: POC Keyword: DOC Type: Keyword type code: theme Descriptive keywords: Keywords: Keyword: inapplicable Type: Keyword type code: place Resource constraints: title: Cite As Constraints: Use limitation: Uta Passow, Marisa Wirth, D. Schulz-Bull. 2017. Roller Tank Experiment for the component-specific Investigation of Oil Incorporation into Marine Oil Snow with a Phytoplankton Culture (Nodularia) (t = 24 hours). Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7TX3CTM Resource constraints: title: CC0 License Legal constraints: Use constraints: Restriction code: licenceUnrestricted Other constraints: This information is released under the Creative Commons license - No Rights Reserved - CC0 1.0 Universal (https://creativecommons.org/publicdomain/zero/1.0/). The person who associated a work with this deed has dedicated the work to the public domain by waiving all of his or her rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law. You can copy, modify, distribute and perform the work, even for commercial purposes, all without asking permission. Resource constraints: title: Liability and Warranty Legal constraints: Other constraints: All materials on this website are made available to GRIIDC and in turn to you "as-is." Content may only be submitted by an individual who represents and warrants that s/he has sufficient rights to be able to make the content available under a CC0 waiver. There is no warranty (expressed or implied) to these materials, their title, accuracy, non-infringement of third party rights, or fitness for any particular purpose, including the performance or results you may obtain from their use. Use these materials at your own risk. Under no circumstances shall GRIIDC be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use or the inability to use either this website or the materials available via this website. If you are dissatisfied with any website feature, content, or terms of use, your sole and exclusive remedy is to discontinue use. Aggregation Info: AggregateInformation: Aggregate Data Set Name: title: Related Publication Citation Citation: Title: Wirth, M. A., Passow, U., Jeschek, J., Hand, I., & Schulz-Bull, D. E. (2018). Partitioning of oil compounds into marine oil snow: Insights into prevailing mechanisms and dispersant effects. Marine Chemistry, 206, 62–73. doi:10.1016/j.marchem.2018.09.007 Date: inapplicable Aggregate Data Set Identifier: title: Related Publication DOI Identifier: Code: Anchor: xlink: https://dx.doi.org/10.1016/j.marchem.2018.09.007 title: DOI doi:10.1016/j.marchem.2018.09.007 Association Type: Association type code: crossReference Language: eng; USA Topic category: Topic category code: oceans Topic category: Topic category code: biota Topic category: Topic category code: environment Extent: Extent: Description: Dataset contains laboratory measurements of oil incorporation into marine snow, no field sampling involved. Supplemental Information: a. Tank number b. Treatment= different treatments and controls were prepared (MilliQ Control = control tank containing only MilliQ water, Plankton Control = control tank containing only the plankton culture, OP = Oil and Plankton treatment, OPC = Oil, Plankton and Corexit treatment) c. Replicate = usually each fraction was sampled once for each tank; however, the PM fractions of the OP and OPC treatments were sampled three times each due to high heterogeneity d. Sample Type = in each treatment three different fractions were sampled: MOS = marine oil snow (aggregated, sinking particles > 1mm), PM = particulate matter < 1mm (unaggregated particles and oil drops), DP = dissolved water phase (everything that passes through a 0.7 µm filter) e. Sample volume (L) f. Entire fraction volume (L) g. DOC = dissolved organic carbon (mg/L) h. POC = particulate organic carbon (mg/L) i. Cell chain length = the total length of all Nodularia strands combined was measured for each sample (mm/mL) j. Naph = Naphthalene (ng/mL) k. Acy = Acenaphthylene (ng/mL) l. Ace = Acenaphthene (ng/mL) m. Fl = Fluorene (ng/mL) n. Phen = Phenanthrene (ng/mL) o. Ant = Anthracene (ng/mL) p. Fluo = Fluoranthene (ng/mL) q. Pyr = Pyrene (ng/mL) r. BaA = Benzo(a)anthracene (ng/mL) s. Chr = Chrysene (ng/mL) t. BbF = Benzo(b)fluoranthene (ng/mL) u. BkF = Benzo(k)fluoranthene (ng/mL) v. BaP = Benzo(a)pyrene (ng/mL) w. Ind = Indeno(1,2,3-c,d)pyrene (ng/mL) x. DBA = Dibenzo(a,h)anthracene (ng/mL) y. BP = Benzo(g,h,i)perylene (ng/mL) z-bd. Cx = n-alkane with x C-atoms and 2x+2 H-atoms |Eight tanks (MilliQ control, Plankton control and three replicates for OP and OPC treatments) were filled bubble free with MilliQ water (MilliQ control) or plankton culture (Plankton control and OP and OPC treatments; salinity 7.2 PSU) and sealed. The MilliQ and Plankton control remained unchanged, but oil or oil and Corexit were slowly injected into the center of the already rotating tanks of the OP and OPC treatments. For the OP treatments, 50 µl of oil (Marlin Platform, Dorado source oil) and 20 µl of MilliQ water were injected. For the OPC treatments, 50 µl of oil, 3 µl of Corexit (Clean Seas) and 20 µl of MilliQ water were used. All tanks were incubated in the dark on the roller tables for 24 hours (until MOS had formed). The temperature was 24.3 °C. For sampling, tanks were carefully removed from the roller tables. All formed MOS > 1mm was collected manually with a cut-off pipette. Afterwards, the tanks were sealed again and the contents mixed. Subsamples from this surrounding seawater fraction were subsequently taken. Both the marine snow slurry and the surrounding seawater were filtered onto 0.7 µm borosilicate glass filters (Whatman, UK). Thereby, three different sample categories were obtained. The filtrate from the surrounding seawater was termed the dissolved phase (DP). The respective filter cake was termed particulate matter < 1 mm (PM). It contained unaggregated marine particles as well as oil drops, since they do not pass through the filter. The filter cake from the marine snow slurry was termed marine oil snow (MOS), the respective filtrate was discarded as it contained predominately DP. The pore water fraction of aggregates is too small to sample in this fraction. Subsamples of the MOS slurry and the surrounding seawater were fixed with Lugols solution and used for cell counting. For n-alkane and PAH analysis, DP samples were mixed with 225 µl of deuterated internal PAH standard solution (LGC Standards, UK). The samples were extracted twice with 10 mL dichloromethane (Prochem, Germany) and once with 10 mL hexane (Prochem, Germany) for 10 minutes each. The extracts were combined, dried over sodium sulfate (Merck, Germany) and concentrated to about 1 mL trough rotary evaporation. Subsequently, the solutions were purified over an aluminum oxide (Merck, Germany) and silica gel (Merck, Germany) column. The column was conditioned with 10 mL toluene (Prochem, Germany) and 10 mL heptane (Prochem, Germany). Analytes were eluted with 15 mL heptane and 30 mL of a heptane toluene mixture (2:1). The purified extracts were again concentrated to 1 mL. Filters (MOS and PM) were extracted with 15 mL dichloromethane and 15 mL hexane in an ultrasonic bath for 2 hours. The solvents were mixed with the same standard solutions. Afterwards, the samples were treated as described for the DP samples. The samples were measured for PAHs using a Trace DSQ GC-MS system (Thermo Scientific, Germany) equipped with an electron impact ionization source and single quadrupole detector that operates in the SIM mode. The compounds were separated with a 60 m DB-5MS column (Agilent, U.S.). The following PAHs were targeted: Naphthalene (Naph), Acenaphtylene (Acy), Acenaphthene (Ace), Fluorene (Fl) Phenanthrene (Phen), Anthracene (Ant), Fluoranthene (Fluo), Pyrene (Pyr), Benz(a)anthracene (BaA), Chrysene (Chr), Benz(b)fluoranthene (BbF), Benz(k)fluoranthene (BkF), Benz(a)pyrene (BaP), Indeno(1,2,3-c,d)pyren (Ind), Dibenzo(a,h)anthracene (DBA) and Benz(g,h,i)perylene (BP). A self-prepared mixture of internal (deuterated) and external PAH standard (LGC Standards, UK) was measured alongside the samples and used for quantification. The samples were measured for alkanes using a Trace GC Ultra system (Thermo Scientific, Germany) equipped with an FID detector. The analytes were separated with a 30 m DB-5MS column (Agilent, U.S.). The n-alkanes from n-Decane (C10H22) to n-Tetracontane (C40H82) were targeted. An external n-alkane standard (Ultra Scientific, U.S.) was measured alongside the samples and used for quantification. The DP samples were measured for dissolved organic carbon (DOC) and the MOS and PM samples for particulate organic carbon (POC). DOC measurements were carried out with a TOC-LCPH/TOC-VCPH TOC-Analyzer (Shimadzu, Germany). Samples were acidified with 80 µL of 2 N HCl to previously purge inorganic carbon. POC measurements were conducted with a varioMICRO cube element analyzer (Elementar Analysensysteme, Germany). Filters were dried for 30 min, acidified with 100 µL 2N HCl and incubated for 30 min. They were subsequently dried, folded and packed in tin containers for the subsequent measurement. Nodularia cells were enumerated in a 3 mL counting chamber for inverted microscopes at 100-fold magnification. The surrounding seawater samples (PM) were used at their full concentration; the MOS slurry samples were diluted 1:30 in Lugols solution. The total length of the Nodularia cell strands in different width size classes was determined in each sample. Sample losses: OP Tank 3 spilled during the experiment. Therefore, no data of this Tank is available.||All samples taken at the end of the experiment (after 24 hours) Tank volume: 1.6 L DP sample volume for oil compounds: 0.3 L DP sample volume for DOC: 0.02 L PM sample volume for oil compounds: 0.15 – 0.5 L filtered PM sample volume for POC: 0.15 – 0.5 L filtered MOS sample volume for oil compounds: 0.06 – 0.18 L of slurry filtered MOS sample volume for POC: 0.015 – 0.04 L of slurry filtered||
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Distribution info: Distribution: Distributor: Distributor: Distributor contact: Responsible party: Organisation name: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC) Contact info: Contact: Phone: Telephone: Voice: 3618253604 Address: Address: Delivery point: 6300 Ocean Drive City: Corpus Christi Administrative area: TX Postal code: 78412 Country: USA Electronic mail address: griidc@gomri.org Online Resource: Online Resource: Linkage: URL: https://data.gulfresearchinitiative.org Role: Role code: distributor Distributor format: Format: Name: csv Version: inapplicable File decompression technique: Distributor transfer options: Digital transfer options: Transfer size: 0.0129 Online: Online Resource: Linkage: URL: https://data.gulfresearchinitiative.org/data/R4.x263.000:0035 Protocol: https Name: Data Landing Page Description: GRIIDC dataset landing page Function: Online function code: information
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Metadata maintenance: Maintenance information: Maintenance and update frequency: unknown Maintenance note: This ISO metadata record was automatically generated from information provided to GRIIDC for dataset: R4.x263.000:0035 on 2020-09-24T10:37:40-05:00
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