Metadata: 
  File identifier: 
      R6.x807.000-0014-metadata.xml
  Language: 
      eng; USA
  Character set: 
    Character set code: 
      utf8
  Hierarchy level: 
    Scope code: 
      dataset
  Metadata author: 
    Responsible party: 
      Individual name: 
          Kathleen (Kathy) Schwehr
      Organisation name: 
          Texas A&M University at Galveston / Marine Sciences Department
      Position name: 
          Associate Research Scientist
      Contact info: 
        Contact: 
          Phone: 
            Telephone: 
              Voice: 
                  4097404452
              Facsimile: 
          Address: 
            Address: 
              Delivery point: 
                  P.O. Box 1675
              City: 
                  Galveston
              Administrative area: 
                  Texas
              Postal code: 
                  77553
              Country: 
                  USA
              Electronic mail address: 
                  schwehrk@tamug.edu
      Role: 
        Role code: 
          pointOfContact
  Date stamp: 
      2019-08-22T15:40:58+00:00
  Metadata standard name: 
      ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
  Metadata standard version: 
      ISO 19115-2:2009(E)
  Dataset URI: 
      https://data.gulfresearchinitiative.org/metadata/R6.x807.000:0014
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  Identification info: 
    Data identification: 
      Citation: 
        Citation: 
          Title: 
              Physiological responses of marine phytoplankton exposed to oil and Corexit in culture experiments
          Alternate title: 
              Algal responses to oil and dispersants
          Date: 
            Date: 
              Date: 
                  2019-07-25
              Date type: 
                Date type code: 
                  publication
          Identifier: 
            Identifier: 
              Code: 
                Anchor: xlink: https://dx.doi.org/doi:10.7266/n7-jehv-5771 title: DOI
                  doi:10.7266/n7-jehv-5771
      Abstract: 
          This work is a continuation of the Tier 1 screening process to identify species of phytoplankton that are 
          sensitive and resilient to oil and Corexit exposure. Cultures were grown of Micromonas pusilla, Tetraselmis 
          astigmatica, Ochromonas sp., and other groups of phytoplankton that are underrepresented in the literature, 
          but still important to the ecology of the Gulf of Mexico. Measurements of growth dynamics and 
          photophysiology help to ascertain how these organisms respond to oil pollution.
      Purpose: 
          This work was conducted to further understand what traits contribute to resistance to oil in marine 
          phytoplankton. The species were selected to test a variety of traits such as motility, mixotrophy, cell size 
          and the presence of a cell wall. This work can allow us to better predict how phytoplankton communities in 
          the Gulf of Mexico might respond to future oil spills, and understand why different taxonomic groups respond 
          so differently to oil exposure.
      Status: 
        Progress code: 
          completed
      Point of contact: 
        Responsible party: 
          Individual name: 
              Antonietta Quigg
          Organisation name: 
              Texas A&M University at Galveston / Marine Biology Department
          Position name: 
              Professor
          Contact info: 
            Contact: 
              Phone: 
                Telephone: 
                  Voice: 
                      4097404990
                  Facsimile: 
              Address: 
                Address: 
                  Delivery point: 
                      P.O. Box 1675
                  City: 
                      Galveston
                  Administrative area: 
                      Texas
                  Postal code: 
                      77553
                  Country: 
                      USA
                  Electronic mail address: 
                      quigga@tamug.edu
          Role: 
            Role code: 
              pointOfContact
      Point of contact: 
        Responsible party: 
          Individual name: 
              Laura Bretherton
          Organisation name: 
              Mount Allison University / Environmental Science
          Position name: 
              Postdoctoral Researcher
          Contact info: 
            Contact: 
              Phone: 
                Telephone: 
                  Voice: 
                  Facsimile: 
              Address: 
                Address: 
                  Delivery point: 
                      67 York St.
                  City: 
                      Sackville
                  Administrative area: 
                      New Brunswick
                  Postal code: 
                      E4L 1E6
                  Country: 
                      CAN
                  Electronic mail address: 
                      lmjbre@gmail.com
          Role: 
            Role code: 
              pointOfContact
      Descriptive keywords: 
        Keywords: 
          Keyword: 
              Phytoplankton
          Keyword: 
              photosynthesis
          Keyword: 
              chlorophyll
          Keyword: 
              Micromonas pusilla
          Keyword: 
              Tetraselmis astigmatica
          Keyword: 
              Ochromonas sp.
          Keyword: 
              Heterocapsa pygmaea
          Keyword: 
              Prorocentrum minimum
          Keyword: 
              Water accommodated fraction (WAF)
          Keyword: 
              Chemically enhanced water accommodated fraction (CEWAF)
          Keyword: 
              Diluted CEWAF (DCEWAF)
          Type: 
            Keyword type code: 
              theme
      Descriptive keywords: 
        Keywords: 
          Keyword: 
            inapplicable
          Type: 
            Keyword type code: 
              place
      Language: 
          eng; USA
      Topic category: 
        Topic category code: 
          oceans
      Topic category: 
        Topic category code: 
          biota
      Topic category: 
        Topic category code: 
          environment
      Extent: 
        Extent: 
          Description: 
              Dataset contains laboratory measurements of growth responses of specific phytoplankton species with 
              varying doses of oil and Corexit, no field sampling involved.
      Supplemental Information: 
          Species: denotes the algal species the data is for (Mp = Micromonas pusilla; Ta = Tetraselmis astigmatica; Os 
          = Ochromonas sp., Hp = Heterocapsa pygmaea; Pm = Prorocentrum minimum)
          Timepoint: units are in days
          Treatment: the conditions the culture was grown in (C = control, f/2 seawater media; O = WAF, Water 
          Accommodated Fraction of oil; M = CEWAF, Chemically Enhanced Water Accommodated Fraction of oil; DM = 
          DCEWAF, Diluted Chemically Enhanced Water Accommodated Fraction of oil)
          Replicate: each treatment was grown in triplicate, labelled A, B and C
          EOE: Estimated Oil Equivalents, oil concentration measured in mg/L
          Chl-a: Chlorophyll-a, measured in µg/L
          Fv/Fm: the photosystem II (PSII) quantum yield, dimensionless
          Sigma: PSII absorption cross-section, measured in Å2 (quanta)-1
          p: PSII connectivity factor, dimensionless
          Tau1: time constant of photosynthetic electron transport on the acceptor side of PSII, measured in µs
          Tau2: time constant of photosynthetic electron transport between PSII and photosystem I (PSI), measured in 
          µs|Treatments were prepared using sterile seawater enriched with f/2 nutrients. WAF treatments were prepared 
          by adding Macondo crude oil to a target concentration of 1mg L-1, and CEWAF treatments were made with a 
          stock solution containing a 20:1 ratio of oil:Corexit. One third of the CEWAF was then diluted until the 
          crude oil concentration was consistent with the WAF to make the DCEWAF treatments. Control treatments were 
          not exposed to either oil or Corexit. The experiments were then set up in 1 L glass bottles and staged in a 
          climate-controlled chamber kept at 19° C. All treatments were grown in triplicate with true biological 
          replication. Every day, samples for EOE (5 mL), chlorophyll, and photophysiology (4 mL) were retrieved from 
          every experimental bottle. To each of the EOE samples, 15 mL of dichloromethane was added, and samples were 
          transferred to a fridge until analysis using a spectrophotofluorometer. The samples for chlorophyll and 
          photophysiology were left to dark-acclimate for ~10 minutes before being transferred into a FIRe 
          (Fluorescence Induction and Relaxation) system (Satlantic, Halifax, Canada). Parameters were collected from 
          both single turnover (ST) and multiple turnover (MT) curves, using 10 acquisitions per sample to reduce 
          noise. The sample was then recovered from the FIRe to measure the relative fluorescence intensity of 
          chlorophyll-a using a Turner fluorometer (Turner Designs, San Jose, USA).||9 mL of culture extracted every 
          24 hours.||
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  Distribution info: 
    Distribution: 
      Distributor: 
        Distributor: 
          Distributor contact: 
            Responsible party: 
              Organisation name: 
                  Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC)
              Contact info: 
                Contact: 
                  Phone: 
                    Telephone: 
                      Voice: 
                          3618253604
                  Address: 
                    Address: 
                      Delivery point: 
                          6300 Ocean Drive
                      City: 
                          Corpus Christi
                      Administrative area: 
                          TX
                      Postal code: 
                          78412
                      Country: 
                          USA
                      Electronic mail address: 
                          griidc@gomri.org
                  Online Resource: 
                    Online Resource: 
                      Linkage: 
                        URL: 
                          https://data.gulfresearchinitiative.org
              Role: 
                Role code: 
                  distributor
          Distributor format: 
            Format: 
              Name: 
                  csv
              Version: 
                inapplicable
              File decompression technique: 
          Distributor transfer options: 
            Digital transfer options: 
              Transfer size: 
                  0.0242
              Online: 
                Online Resource: 
                  Linkage: 
                    URL: 
                      https://data.gulfresearchinitiative.org/data/R6.x807.000:0014
                  Protocol: 
                      https
                  Name: 
                      Data Landing Page
                  Description: 
                      GRIIDC dataset landing page
                  Function: 
                    Online function code: 
                      information
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  Metadata maintenance: 
    Maintenance information: 
      Maintenance and update frequency: 
        unknown
      Maintenance note: 
          This ISO metadata record was automatically generated from information provided to GRIIDC for dataset: 
          R6.x807.000:0014 on 2020-04-03T15:49:30-05:00
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