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Physiological responses of marine phytoplankton exposed to oil and Corexit in culture experimentsAggregation and Degradation of Dispersants and Oil by Microbial Exopolymers 2 (ADDOMEx-2)
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Suggested Citation:
Laura Bretherton, Andrew Irwin, Zoe Finkel, Antonietta Quigg. 2019. Physiological responses of marine phytoplankton exposed to oil and Corexit in culture experiments. Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/n7-jehv-5771
Abstract:
This work is a continuation of the Tier 1 screening process to identify species of phytoplankton that are sensitive and resilient to oil and Corexit exposure. Cultures were grown of Micromonas pusilla, Tetraselmis astigmatica, Ochromonas sp., and other groups of phytoplankton that are underrepresented in the literature, but still important to the ecology of the Gulf of Mexico. Measurements of growth dynamics and photophysiology help to ascertain how these organisms respond to oil pollution.
Purpose:
This work was conducted to further understand what traits contribute to resistance to oil in marine phytoplankton. The species were selected to test a variety of traits such as motility, mixotrophy, cell size and the presence of a cell wall. This work can allow us to better predict how phytoplankton communities in the Gulf of Mexico might respond to future oil spills, and understand why different taxonomic groups respond so differently to oil exposure.
Theme Keywords:
Phytoplankton, photosynthesis, chlorophyll, Micromonas pusilla, Tetraselmis astigmatica, Ochromonas sp., Heterocapsa pygmaea, Prorocentrum minimum, Water accommodated fraction (WAF), Chemically enhanced water accommodated fraction (CEWAF), Diluted CEWAF (DCEWAF)
File Format:
csv
Filename:
Screening Part 2 GRIIDC BRETHERTON.csv (24.24 KB)
Dataset Downloads:
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Physiological responses of marine phytoplankton exposed to oil and Corexit in culture experiments
Identification Information
Distribution Information
Metadata Maintenance Information
Metadata:
File identifier:
R6.x807.000-0014-metadata.xml
Language:
eng; USA
Character set:
Character set code:
utf8
Hierarchy level:
Scope code:
dataset
Metadata author:
Responsible party:
Individual name:
Kathleen (Kathy) Schwehr
Organisation name:
Texas A&M University at Galveston / Marine Sciences Department
Position name:
Associate Research Scientist
Contact info:
Contact:
Phone:
Telephone:
Voice:
4097404452
Facsimile:
Address:
Address:
Delivery point:
P.O. Box 1675
City:
Galveston
Administrative area:
Texas
Postal code:
77553
Country:
USA
Electronic mail address:
schwehrk@tamug.edu
Role:
Role code:
pointOfContact
Date stamp:
2019-08-22T15:40:58+00:00
Metadata standard name:
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
Metadata standard version:
ISO 19115-2:2009(E)
Dataset URI:
https://data.gulfresearchinitiative.org/metadata/R6.x807.000:0014
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Identification info:
Data identification:
Citation:
Citation:
Title:
Physiological responses of marine phytoplankton exposed to oil and Corexit in culture experiments
Alternate title:
Algal responses to oil and dispersants
Date:
Date:
Date:
2019-08-22
Date type:
Date type code:
publication
Identifier:
Identifier:
Code:
Anchor: xlink: https://dx.doi.org/10.7266/n7-jehv-5771 title: DOI
doi:10.7266/n7-jehv-5771
Abstract:
This work is a continuation of the Tier 1 screening process to identify species of phytoplankton that are
sensitive and resilient to oil and Corexit exposure. Cultures were grown of Micromonas pusilla, Tetraselmis
astigmatica, Ochromonas sp., and other groups of phytoplankton that are underrepresented in the literature,
but still important to the ecology of the Gulf of Mexico. Measurements of growth dynamics and
photophysiology help to ascertain how these organisms respond to oil pollution.
Purpose:
This work was conducted to further understand what traits contribute to resistance to oil in marine
phytoplankton. The species were selected to test a variety of traits such as motility, mixotrophy, cell size
and the presence of a cell wall. This work can allow us to better predict how phytoplankton communities in
the Gulf of Mexico might respond to future oil spills, and understand why different taxonomic groups respond
so differently to oil exposure.
Status:
Progress code:
completed
Point of contact:
Responsible party:
Individual name:
Antonietta Quigg
Organisation name:
Texas A&M University at Galveston / Marine Biology Department
Position name:
Professor
Contact info:
Contact:
Phone:
Telephone:
Voice:
4097404990
Facsimile:
Address:
Address:
Delivery point:
P.O. Box 1675
City:
Galveston
Administrative area:
Texas
Postal code:
77553
Country:
USA
Electronic mail address:
quigga@tamug.edu
Role:
Role code:
pointOfContact
Point of contact:
Responsible party:
Individual name:
Laura Bretherton
Organisation name:
Mount Allison University / Environmental Science
Position name:
Postdoctoral Researcher
Contact info:
Contact:
Phone:
Telephone:
Voice:
Facsimile:
Address:
Address:
Delivery point:
67 York St.
City:
Sackville
Administrative area:
New Brunswick
Postal code:
E4L 1E6
Country:
CAN
Electronic mail address:
lmjbre@gmail.com
Role:
Role code:
pointOfContact
Descriptive keywords:
Keywords:
Keyword:
Phytoplankton
Keyword:
photosynthesis
Keyword:
chlorophyll
Keyword:
Micromonas pusilla
Keyword:
Tetraselmis astigmatica
Keyword:
Ochromonas sp.
Keyword:
Heterocapsa pygmaea
Keyword:
Prorocentrum minimum
Keyword:
Water accommodated fraction (WAF)
Keyword:
Chemically enhanced water accommodated fraction (CEWAF)
Keyword:
Diluted CEWAF (DCEWAF)
Type:
Keyword type code:
theme
Descriptive keywords:
Keywords:
Keyword:
inapplicable
Type:
Keyword type code:
place
Resource constraints: title: Cite As
Constraints:
Use limitation:
Laura Bretherton, Andrew Irwin, Zoe Finkel, Antonietta Quigg. 2019. Physiological responses of marine
phytoplankton exposed to oil and Corexit in culture experiments. Distributed by: Gulf of Mexico Research
Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M
University–Corpus Christi. doi:10.7266/n7-jehv-5771
Resource constraints: title: CC0 License
Legal constraints:
Use constraints:
Restriction code:
licenceUnrestricted
Other constraints:
This information is released under the Creative Commons license - No Rights Reserved - CC0 1.0 Universal
(https://creativecommons.org/publicdomain/zero/1.0/). The person who associated a work with this deed
has dedicated the work to the public domain by waiving all of his or her rights to the work worldwide
under copyright law, including all related and neighboring rights, to the extent allowed by law. You can
copy, modify, distribute and perform the work, even for commercial purposes, all without asking
permission.
Resource constraints: title: Liability and Warranty
Legal constraints:
Other constraints:
All materials on this website are made available to GRIIDC and in turn to you "as-is." Content may only be
submitted by an individual who represents and warrants that s/he has sufficient rights to be able to
make the content available under a CC0 waiver. There is no warranty (expressed or implied) to these
materials, their title, accuracy, non-infringement of third party rights, or fitness for any particular
purpose, including the performance or results you may obtain from their use. Use these materials at your
own risk. Under no circumstances shall GRIIDC be liable for any direct, incidental, special,
consequential, indirect, or punitive damages that result from the use or the inability to use either
this website or the materials available via this website. If you are dissatisfied with any website
feature, content, or terms of use, your sole and exclusive remedy is to discontinue use.
Aggregation Info:
AggregateInformation:
Aggregate Data Set Name: title: Related Publication Citation
Citation:
Title:
Bretherton, L., Hillhouse, J., Kamalanathan, M., Finkel, Z. V., Irwin, A. J., & Quigg, A. (2020).
Trait-dependent variability of the response of marine phytoplankton to oil and dispersant exposure.
Marine Pollution Bulletin, 153, 110906. doi:10.1016/j.marpolbul.2020.110906
Date:
inapplicable
Aggregate Data Set Identifier: title: Related Publication DOI
Identifier:
Code:
Anchor: xlink: https://dx.doi.org/10.1016/j.marpolbul.2020.110906 title: DOI
doi:10.1016/j.marpolbul.2020.110906
Association Type:
Association type code:
crossReference
Language:
eng; USA
Topic category:
Topic category code:
oceans
Topic category:
Topic category code:
biota
Topic category:
Topic category code:
environment
Extent:
Extent:
Description:
Dataset contains laboratory measurements of growth responses of specific phytoplankton species with
varying doses of oil and Corexit, no field sampling involved.
Supplemental Information:
Species: denotes the algal species the data is for (Mp = Micromonas pusilla; Ta = Tetraselmis astigmatica; Os
= Ochromonas sp., Hp = Heterocapsa pygmaea; Pm = Prorocentrum minimum)
Timepoint: units are in days
Treatment: the conditions the culture was grown in (C = control, f/2 seawater media; O = WAF, Water
Accommodated Fraction of oil; M = CEWAF, Chemically Enhanced Water Accommodated Fraction of oil; DM =
DCEWAF, Diluted Chemically Enhanced Water Accommodated Fraction of oil)
Replicate: each treatment was grown in triplicate, labelled A, B and C
EOE: Estimated Oil Equivalents, oil concentration measured in mg/L
Chl-a: Chlorophyll-a, measured in µg/L
Fv/Fm: the photosystem II (PSII) quantum yield, dimensionless
Sigma: PSII absorption cross-section, measured in Å2 (quanta)-1
p: PSII connectivity factor, dimensionless
Tau1: time constant of photosynthetic electron transport on the acceptor side of PSII, measured in µs
Tau2: time constant of photosynthetic electron transport between PSII and photosystem I (PSI), measured in
µs|Treatments were prepared using sterile seawater enriched with f/2 nutrients. WAF treatments were prepared
by adding Macondo crude oil to a target concentration of 1mg L-1, and CEWAF treatments were made with a
stock solution containing a 20:1 ratio of oil:Corexit. One third of the CEWAF was then diluted until the
crude oil concentration was consistent with the WAF to make the DCEWAF treatments. Control treatments were
not exposed to either oil or Corexit. The experiments were then set up in 1 L glass bottles and staged in a
climate-controlled chamber kept at 19° C. All treatments were grown in triplicate with true biological
replication. Every day, samples for EOE (5 mL), chlorophyll, and photophysiology (4 mL) were retrieved from
every experimental bottle. To each of the EOE samples, 15 mL of dichloromethane was added, and samples were
transferred to a fridge until analysis using a spectrophotofluorometer. The samples for chlorophyll and
photophysiology were left to dark-acclimate for ~10 minutes before being transferred into a FIRe
(Fluorescence Induction and Relaxation) system (Satlantic, Halifax, Canada). Parameters were collected from
both single turnover (ST) and multiple turnover (MT) curves, using 10 acquisitions per sample to reduce
noise. The sample was then recovered from the FIRe to measure the relative fluorescence intensity of
chlorophyll-a using a Turner fluorometer (Turner Designs, San Jose, USA).||9 mL of culture extracted every
24 hours.||
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Distribution info:
Distribution:
Distributor:
Distributor:
Distributor contact:
Responsible party:
Organisation name:
Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC)
Contact info:
Contact:
Phone:
Telephone:
Voice:
3618253604
Address:
Address:
Delivery point:
6300 Ocean Drive
City:
Corpus Christi
Administrative area:
TX
Postal code:
78412
Country:
USA
Electronic mail address:
griidc@gomri.org
Online Resource:
Online Resource:
Linkage:
URL:
https://data.gulfresearchinitiative.org
Role:
Role code:
distributor
Distributor format:
Format:
Name:
csv
Version:
inapplicable
File decompression technique:
Distributor transfer options:
Digital transfer options:
Transfer size:
0.0242
Online:
Online Resource:
Linkage:
URL:
https://data.gulfresearchinitiative.org/data/R6.x807.000:0014
Protocol:
https
Name:
Data Landing Page
Description:
GRIIDC dataset landing page
Function:
Online function code:
information
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Metadata maintenance:
Maintenance information:
Maintenance and update frequency:
unknown
Maintenance note:
This ISO metadata record was automatically generated from information provided to GRIIDC for dataset:
R6.x807.000:0014 on 2020-09-27T03:16:29-05:00
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