Physiological responses of marine phytoplankton exposed to oil and Corexit in culture experiments

Aggregation and Degradation of Dispersants and Oil by Microbial Exopolymers 2 (ADDOMEx-2)

DOI:
10.7266/n7-jehv-5771
 
UDI:
R6.x807.000:0014
Last Update:
Jul 25 2019 20:11 UTC
 
Dataset Author(s):
Laura Bretherton, Andrew Irwin, Zoe Finkel, Antonietta Quigg
Point of Contact:
Quigg, Antonietta
Texas A&M University at Galveston / Marine Biology Department
P.O. Box 1675
Galveston, Texas  77553
USA
quigga@tamug.edu
Funding Source:
RFP-VI
 
Extent Description:
Dataset contains laboratory measurements of growth responses of specific phytoplankton species with varying doses of oil and Corexit, no field sampling involved.

Identified Submitted In-Review Available
3 3 3 3

Suggested Citation:

Laura Bretherton, Andrew Irwin, Zoe Finkel, Antonietta Quigg. 2019. Physiological responses of marine phytoplankton exposed to oil and Corexit in culture experiments. Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/n7-jehv-5771

Abstract:

This work is a continuation of the Tier 1 screening process to identify species of phytoplankton that are sensitive and resilient to oil and Corexit exposure. Cultures were grown of Micromonas pusilla, Tetraselmis astigmatica, Ochromonas sp., and other groups of phytoplankton that are underrepresented in the literature, but still important to the ecology of the Gulf of Mexico. Measurements of growth dynamics and photophysiology help to ascertain how these organisms respond to oil pollution.

Purpose:

This work was conducted to further understand what traits contribute to resistance to oil in marine phytoplankton. The species were selected to test a variety of traits such as motility, mixotrophy, cell size and the presence of a cell wall. This work can allow us to better predict how phytoplankton communities in the Gulf of Mexico might respond to future oil spills, and understand why different taxonomic groups respond so differently to oil exposure.

Theme Keywords:

Phytoplankton, photosynthesis, chlorophyll, Micromonas pusilla, Tetraselmis astigmatica, Ochromonas sp., Heterocapsa pygmaea, Prorocentrum minimum, Water accommodated fraction (WAF), Chemically enhanced water accommodated fraction (CEWAF), Diluted CEWAF (DCEWAF)

File Format:

csv

Filename:

Screening Part 2 GRIIDC BRETHERTON.csv (24.24 KB)

Dataset Downloads:

0

Physiological responses of marine phytoplankton exposed to oil and Corexit in culture experiments



Identification Information
Distribution Information
Metadata Maintenance Information

Metadata: 
  File identifier: 
      R6.x807.000-0014-metadata.xml
  Language: 
      eng; USA
  Character set: 
    Character set code: 
      utf8
  Hierarchy level: 
    Scope code: 
      dataset
  Metadata author: 
    Responsible party: 
      Individual name: 
          Kathleen (Kathy) Schwehr
      Organisation name: 
          Texas A&M University at Galveston / Marine Sciences Department
      Position name: 
          Associate Research Scientist
      Contact info: 
        Contact: 
          Phone: 
            Telephone: 
              Voice: 
                  4097404452
              Facsimile: 
          Address: 
            Address: 
              Delivery point: 
                  P.O. Box 1675
              City: 
                  Galveston
              Administrative area: 
                  Texas
              Postal code: 
                  77553
              Country: 
                  USA
              Electronic mail address: 
                  schwehrk@tamug.edu
      Role: 
        Role code: 
          pointOfContact
  Date stamp: 
      2019-08-22T15:40:58+00:00
  Metadata standard name: 
      ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
  Metadata standard version: 
      ISO 19115-2:2009(E)
  Dataset URI: 
      https://data.gulfresearchinitiative.org/metadata/R6.x807.000:0014
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Identification info: Data identification: Citation: Citation: Title: Physiological responses of marine phytoplankton exposed to oil and Corexit in culture experiments Alternate title: Algal responses to oil and dispersants Date: Date: Date: 2019-08-22 Date type: Date type code: publication Identifier: Identifier: Code: Anchor: xlink: https://dx.doi.org/10.7266/n7-jehv-5771 title: DOI doi:10.7266/n7-jehv-5771 Abstract: This work is a continuation of the Tier 1 screening process to identify species of phytoplankton that are sensitive and resilient to oil and Corexit exposure. Cultures were grown of Micromonas pusilla, Tetraselmis astigmatica, Ochromonas sp., and other groups of phytoplankton that are underrepresented in the literature, but still important to the ecology of the Gulf of Mexico. Measurements of growth dynamics and photophysiology help to ascertain how these organisms respond to oil pollution. Purpose: This work was conducted to further understand what traits contribute to resistance to oil in marine phytoplankton. The species were selected to test a variety of traits such as motility, mixotrophy, cell size and the presence of a cell wall. This work can allow us to better predict how phytoplankton communities in the Gulf of Mexico might respond to future oil spills, and understand why different taxonomic groups respond so differently to oil exposure. Status: Progress code: completed Point of contact: Responsible party: Individual name: Antonietta Quigg Organisation name: Texas A&M University at Galveston / Marine Biology Department Position name: Professor Contact info: Contact: Phone: Telephone: Voice: 4097404990 Facsimile: Address: Address: Delivery point: P.O. Box 1675 City: Galveston Administrative area: Texas Postal code: 77553 Country: USA Electronic mail address: quigga@tamug.edu Role: Role code: pointOfContact Point of contact: Responsible party: Individual name: Laura Bretherton Organisation name: Mount Allison University / Environmental Science Position name: Postdoctoral Researcher Contact info: Contact: Phone: Telephone: Voice: Facsimile: Address: Address: Delivery point: 67 York St. City: Sackville Administrative area: New Brunswick Postal code: E4L 1E6 Country: CAN Electronic mail address: lmjbre@gmail.com Role: Role code: pointOfContact Descriptive keywords: Keywords: Keyword: Phytoplankton Keyword: photosynthesis Keyword: chlorophyll Keyword: Micromonas pusilla Keyword: Tetraselmis astigmatica Keyword: Ochromonas sp. Keyword: Heterocapsa pygmaea Keyword: Prorocentrum minimum Keyword: Water accommodated fraction (WAF) Keyword: Chemically enhanced water accommodated fraction (CEWAF) Keyword: Diluted CEWAF (DCEWAF) Type: Keyword type code: theme Descriptive keywords: Keywords: Keyword: inapplicable Type: Keyword type code: place Resource constraints: title: Cite As Constraints: Use limitation: Laura Bretherton, Andrew Irwin, Zoe Finkel, Antonietta Quigg. 2019. Physiological responses of marine phytoplankton exposed to oil and Corexit in culture experiments. Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/n7-jehv-5771 Resource constraints: title: CC0 License Legal constraints: Use constraints: Restriction code: licenceUnrestricted Other constraints: This information is released under the Creative Commons license - No Rights Reserved - CC0 1.0 Universal (https://creativecommons.org/publicdomain/zero/1.0/). The person who associated a work with this deed has dedicated the work to the public domain by waiving all of his or her rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law. You can copy, modify, distribute and perform the work, even for commercial purposes, all without asking permission. Resource constraints: title: Liability and Warranty Legal constraints: Other constraints: All materials on this website are made available to GRIIDC and in turn to you "as-is." Content may only be submitted by an individual who represents and warrants that s/he has sufficient rights to be able to make the content available under a CC0 waiver. There is no warranty (expressed or implied) to these materials, their title, accuracy, non-infringement of third party rights, or fitness for any particular purpose, including the performance or results you may obtain from their use. Use these materials at your own risk. Under no circumstances shall GRIIDC be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use or the inability to use either this website or the materials available via this website. If you are dissatisfied with any website feature, content, or terms of use, your sole and exclusive remedy is to discontinue use. Aggregation Info: AggregateInformation: Aggregate Data Set Name: title: Related Publication Citation Citation: Title: Bretherton, L., Hillhouse, J., Kamalanathan, M., Finkel, Z. V., Irwin, A. J., & Quigg, A. (2020). Trait-dependent variability of the response of marine phytoplankton to oil and dispersant exposure. Marine Pollution Bulletin, 153, 110906. doi:10.1016/j.marpolbul.2020.110906 Date: inapplicable Aggregate Data Set Identifier: title: Related Publication DOI Identifier: Code: Anchor: xlink: https://dx.doi.org/10.1016/j.marpolbul.2020.110906 title: DOI doi:10.1016/j.marpolbul.2020.110906 Association Type: Association type code: crossReference Language: eng; USA Topic category: Topic category code: oceans Topic category: Topic category code: biota Topic category: Topic category code: environment Extent: Extent: Description: Dataset contains laboratory measurements of growth responses of specific phytoplankton species with varying doses of oil and Corexit, no field sampling involved. Supplemental Information: Species: denotes the algal species the data is for (Mp = Micromonas pusilla; Ta = Tetraselmis astigmatica; Os = Ochromonas sp., Hp = Heterocapsa pygmaea; Pm = Prorocentrum minimum) Timepoint: units are in days Treatment: the conditions the culture was grown in (C = control, f/2 seawater media; O = WAF, Water Accommodated Fraction of oil; M = CEWAF, Chemically Enhanced Water Accommodated Fraction of oil; DM = DCEWAF, Diluted Chemically Enhanced Water Accommodated Fraction of oil) Replicate: each treatment was grown in triplicate, labelled A, B and C EOE: Estimated Oil Equivalents, oil concentration measured in mg/L Chl-a: Chlorophyll-a, measured in µg/L Fv/Fm: the photosystem II (PSII) quantum yield, dimensionless Sigma: PSII absorption cross-section, measured in Å2 (quanta)-1 p: PSII connectivity factor, dimensionless Tau1: time constant of photosynthetic electron transport on the acceptor side of PSII, measured in µs Tau2: time constant of photosynthetic electron transport between PSII and photosystem I (PSI), measured in µs|Treatments were prepared using sterile seawater enriched with f/2 nutrients. WAF treatments were prepared by adding Macondo crude oil to a target concentration of 1mg L-1, and CEWAF treatments were made with a stock solution containing a 20:1 ratio of oil:Corexit. One third of the CEWAF was then diluted until the crude oil concentration was consistent with the WAF to make the DCEWAF treatments. Control treatments were not exposed to either oil or Corexit. The experiments were then set up in 1 L glass bottles and staged in a climate-controlled chamber kept at 19° C. All treatments were grown in triplicate with true biological replication. Every day, samples for EOE (5 mL), chlorophyll, and photophysiology (4 mL) were retrieved from every experimental bottle. To each of the EOE samples, 15 mL of dichloromethane was added, and samples were transferred to a fridge until analysis using a spectrophotofluorometer. The samples for chlorophyll and photophysiology were left to dark-acclimate for ~10 minutes before being transferred into a FIRe (Fluorescence Induction and Relaxation) system (Satlantic, Halifax, Canada). Parameters were collected from both single turnover (ST) and multiple turnover (MT) curves, using 10 acquisitions per sample to reduce noise. The sample was then recovered from the FIRe to measure the relative fluorescence intensity of chlorophyll-a using a Turner fluorometer (Turner Designs, San Jose, USA).||9 mL of culture extracted every 24 hours.||
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Distribution info: Distribution: Distributor: Distributor: Distributor contact: Responsible party: Organisation name: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC) Contact info: Contact: Phone: Telephone: Voice: 3618253604 Address: Address: Delivery point: 6300 Ocean Drive City: Corpus Christi Administrative area: TX Postal code: 78412 Country: USA Electronic mail address: griidc@gomri.org Online Resource: Online Resource: Linkage: URL: https://data.gulfresearchinitiative.org Role: Role code: distributor Distributor format: Format: Name: csv Version: inapplicable File decompression technique: Distributor transfer options: Digital transfer options: Transfer size: 0.0242 Online: Online Resource: Linkage: URL: https://data.gulfresearchinitiative.org/data/R6.x807.000:0014 Protocol: https Name: Data Landing Page Description: GRIIDC dataset landing page Function: Online function code: information
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Metadata maintenance: Maintenance information: Maintenance and update frequency: unknown Maintenance note: This ISO metadata record was automatically generated from information provided to GRIIDC for dataset: R6.x807.000:0014 on 2020-07-02T14:34:38-05:00
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