Abstract:

This dataset contains hepatic polycyclic aromatic hydrocarbons (PAHs) in Gulf and Southern Hake collected aboard multiple R/V Weatherbird II cruises in the Gulf of Mexico from 2012-08-21 to 2015-09-24. PAH was extracted from liver samples using a modified QuEChERS (Bond Elut, Agilent Technologies, Santa Clara, CA, USA) method optimized specifically for these study species. Extracts were analyzed using Agilent’s 7890B gas chromatograph (GC) coupled to a 7010 tandem mass spectrometer (MS/MS) operating in multiple reactions mode (MRM). The dataset contains the location, date, biometrics, and the total concentration of polycyclic aromatic hydrocarbon (PAHs) in livers. The cruise documentation was provided for the R/V Weatherbird II cruises WB1304 (2012-08-13 to 2012-08-25), WB1403 (2013-08-15 to 2013-08-30), WB1502 (2014-08-05 to 2014-08-20), WB1602 (2015-08-15 to 2015-08-30), and WB1603 (2015-09-10 to 2015-10-02), led by chief scientists Dr. Steven Murawski and Dr. David Hollander.

Suggested Citation:

Erin Pulster, Steven Murawski, Rachel Struch. 2019. Hepatic polycyclic aromatic hydrocarbons (PAHs) in Gulf and Southern Hake collected aboard multiple R/V Weatherbird II cruises in the Gulf of Mexico from 2012-08-21 to 2015-09-24. Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/n7-tv8b-7h23

Publications:

Struch, R. E., Pulster, E. L., Schreier, A. M., & Murawski, S. A. (2019). Hepatobiliary Analyses Suggest Chronic PAH Exposure in Hakes ( Urophycis spp.) Following the Deepwater Horizon Oil Spill. Environmental Toxicology and Chemistry. doi:10.1002/etc.4596

Purpose:

Data were collected to characterize the impacts of the Deepwater Horizon oil spill on benthic species.

Data Parameters and Units:

The worksheet "Biometrics": Cruise (C-IMAGE I, C-IMAGE II); vESSEL (R/V Weatherbird II); Principal Investigator; Month ,Day, Year, (MM DD YYYY); Latitude (decimal degrees), Longitude (decimal degrees), Species, Transect/Station, Fish #, Disease, Sex, SL (Standard length, cm), TL (Total length, cm), TTL Wt. (Total weight, kg), Liver Wt. (kg), GI Wt. (Gastrointestinal tract weight, kg), Gonad Wt. (kg), Subarea, Country, Gonad SI (Gonadal Somatic Index), HSI (Hepatosomatic Index), GI -SI (Gastrointestinal Somatic Index), Fulton's K (Fulton's Condition Factor), Comments. The worksheet "Hepatic PAHs": Year, Field ID, Species, Transect-Station, Fish#, MDL, Naphthalene (ng/g wet wt), C1 Naphthalene (ng/g wet wt), C2 Naphthalene (ng/g wet wt), C3 Naphthalene (ng/g wet wt), C4 Naphthalene (ng/g wet wt), Acenaphthylene (ng/g wet wt), Acenaphthene (ng/g wet wt), Fluorene (ng/g wet wt), C1 Fluorene (ng/g wet wt), C2 Fluorene (ng/g wet wt), C3 Fluorene (ng/g wet wt), C4 Fluorene (ng/g wet wt), Dibenzothiophene (ng/g wet wt), C1 Dibenzothiophene (ng/g wet wt) C2 Dibenzothiophene (ng/g wet wt), C3 Dibenzothiophene (ng/g wet wt), C4 Dibenzothiophene (ng/g wet wt), Phenanthrene (ng/g wet wt), Anthracene (ng/g wet wt), C1 Phenanthrene/Anthracene (ng/g wet wt), C2 Phenanthrene/Anthracene (ng/g wet wt), C3 Phenanthrene/Anthracene (ng/g wet wt), C4 Phenanthrene/Anthracene (ng/g wet wt), C5 Phenanthrene/Anthracene (ng/g wet wt), Fluoranthene (ng/g wet wt),Pyrene (ng/g wet wt), C1 Fluoranthene/Pyrene (ng/g wet wt), C2 Fluoranthene/Pyrene (ng/g wet wt), C3 Fluoranthene/Pyrene (ng/g wet wt), C4 Fluoranthene/Pyrene (ng/g wet wt), C5 Fluoranthene/Pyrene (ng/g wet wt), Benzo[A]anthracene (ng/g wet wt), Chrysene (ng/g wet wt), C1 Benzo[A]anthracene/Chrysene (ng/g wet wt), C2 Benzo[A]anthracene/Chrysene (ng/g wet wt), C3 Benzo[A]anthracene/Chrysene (ng/g wet wt), C4 Benzo[A]anthracene/Chrysene (ng/g wet wt), C5 Benzo[A]anthracene/Chrysene (ng/g wet wt), Benzo[b]fluoranthene (ng/g wet wt), Benzo[k]fluoranthene (ng/g wet wt), Benzo[e]pyrene (ng/g wet wt) Benzo[a]pyrene (ng/g wet wt), Perylene (ng/g wet wt), Indeno[1,2,3,-cd]pyrene (ng/g wet wt), Dibenzo[a,h]anthracene (ng/g wet wt) Benzo[g,h,i]perylene (ng/g wet wt), SumLivPAH (ng/g wet wt). The worksheet "Definitions" contains the definitions of some of the parameters in the other worksheets, and are as follows: Nap Eq. (ng/g bile): Naphthalene equivalents in ng per g of bile Phn Eq. (ng/g bile): Phenanthrene equivalents in ng per g of bile BaP Eq. (ng/g bile): Benzo[a]pyrene equivalents in ng per g of bile Liver tPAHs (ng/g w.w.): Liver concentrations of total PAHs in ng per g of tissue wet weight SL (cm): Standard length in centimeters TL (cm) : Total length in centimeters TTL Wt. (kg): Total weight in kilograms Liver Wt. (kg): Liver weight in kilograms GI Wt. (kg): Gastrointestinal tract weight in kilograms Gonad Wt. (kg): Gonad weight in kilograms Gonad SI: Gonadal Somatic Index H.S.I.: HepatoSomatic Index GI –SI: Gastrointestinal Somatic Index Fulton's K: Fulton's Condition Factor (K) MDL: Method Detection Limit (1 ng/g) Subareas/regions: North Central Region of the Gulf of Mexico (NC), West Florida Shelf (WFS), Southwest Region of the Gulf of Mexico (SW).

Methods:

PAHs were extracted from Liver samples using a modified QuEChERS (Bond Elut, Agilent Technologies, Santa Clara, CA, USA) method optimized specifically for these study species. Extracts were analyzed using Agilent’s 7890B gas chromatograph (GC) coupled to a 7010 tandem mass spectrometer (MS/MS) operating in multiple reactions mode (MRM).

Error Analysis:

A performance-based QA/QC program was implemented to ensure acceptable levels of analytical accuracy and precision following the EPA’s Method 8270D and the National Oceanic and Atmospheric Administration’s quality assurance and quality control plan for DWH Natural Resource Damage Assessment. The program included the analysis of solvent blanks, procedural blanks, sample replicates, matrix spikes, blank spikes, and matrix-matched standards.

Individual Files: