The effect of oil-exposure on urea and ammonia transporters in mahi-mahi (Coryphaena hippurus)

Relationship of Effects of Cardiac Outcomes in Fish for Validation of Ecological Risk II (RECOVER II)

DOI:
10.7266/n7-q77z-bm73
 
UDI:
R6.x804.000:0032
Last Update:
Mar 20 2019 16:12 UTC
 
Dataset Author(s):
Wang, Y.
Point of Contact:
Grosell, Martin
University of Miami / Rosenstiel School of Marine and Atmospheric Science
4600 Rickenbacker Causeway
Miami, Florida  33149
USA
mgrosell@rsmas.miami.edu
Funding Source:
RFP-VI
 
Extent Description:
Dataset contains laboratory measurements of ammonia and urea concentrations, no field sampling involved.

Identified Submitted In-Review Available
3 3 3 3

Suggested Citation:

Wang, Y.. 2019. The effect of oil-exposure on urea and ammonia transporters in mahi-mahi (Coryphaena hippurus). Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/n7-q77z-bm73

Abstract:

Embryos were exposed to high-energy water accommodated fractions (HEWAFs). The ammonia and urea transporters gene expression changes and ammonia and urea excretion rates were measured.

Purpose:

Testing the effects of oil exposure on tissue ammonia and urea concentrations, ammonia and urea excretion rates, and mRNA changes of relevant transporters during the development.

Theme Keywords:

nitrogenous waste, Ammonia, urea, mahi-mahi, coryphaena hippurus, transporters, waste excretion, tissue concentrations, high-energy water accommodated fractions (HEWAFs)

File Format:

xlsx

Filename:

R6x8040000032 mahi urea and ammonia transporters.zip (2.95 MB)

Dataset Downloads:

1

The effect of oil-exposure on urea and ammonia transporters in mahi-mahi (Coryphaena hippurus)



Identification Information
Distribution Information
Metadata Maintenance Information

Metadata: 
  File identifier: 
      R6.x804.000-0032-metadata.xml
  Language: 
      eng; USA
  Character set: 
    Character set code: 
      utf8
  Hierarchy level: 
    Scope code: 
      dataset
  Metadata author: 
    Responsible party: 
      Individual name: 
          Madeline Munoz-Bustamante
      Organisation name: 
          University of Miami / Rosenstiel School of Marine and Atmospheric Science
      Position name: 
          Program Manager
      Contact info: 
        Contact: 
          Phone: 
            Telephone: 
              Voice: 
              Facsimile: 
          Address: 
            Address: 
              Delivery point: 
                  4600 Rickenbacker Cswy
              City: 
                  Miami
              Administrative area: 
                  Florida
              Postal code: 
                  33149
              Country: 
                  USA
              Electronic mail address: 
                  mbustamante@rsmas.miami.edu
      Role: 
        Role code: 
          pointOfContact
  Date stamp: 
      2021-05-04T20:04:58+00:00
  Metadata standard name: 
      ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
  Metadata standard version: 
      ISO 19115-2:2009(E)
  Dataset URI: 
      https://data.gulfresearchinitiative.org/metadata/R6.x804.000:0032
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Identification info: Data identification: Citation: Citation: Title: The effect of oil-exposure on urea and ammonia transporters in mahi-mahi (Coryphaena hippurus) Alternate title: Date: Date: Date: 2019-06-26 Date type: Date type code: publication Identifier: Identifier: Code: Anchor: xlink: https://dx.doi.org/10.7266/n7-q77z-bm73 title: DOI doi:10.7266/n7-q77z-bm73 Abstract: Embryos were exposed to high-energy water accommodated fractions (HEWAFs). The ammonia and urea transporters gene expression changes and ammonia and urea excretion rates were measured. Purpose: Testing the effects of oil exposure on tissue ammonia and urea concentrations, ammonia and urea excretion rates, and mRNA changes of relevant transporters during the development. Status: Progress code: completed Point of contact: Responsible party: Individual name: Martin Grosell Organisation name: University of Miami / Rosenstiel School of Marine and Atmospheric Science Position name: Professor Contact info: Contact: Phone: Telephone: Voice: 3054214623 Facsimile: Address: Address: Delivery point: 4600 Rickenbacker Causeway City: Miami Administrative area: Florida Postal code: 33149 Country: USA Electronic mail address: mgrosell@rsmas.miami.edu Role: Role code: pointOfContact Point of contact: Responsible party: Individual name: Yadong Wang Organisation name: University of Miami / Rosenstiel School of Marine and Atmospheric Science Position name: Graduate Student Contact info: Contact: Phone: Telephone: Voice: Facsimile: Address: Address: Delivery point: 4600 Rickenbacker Causeway City: Miami Administrative area: Florida Postal code: 33149 Country: USA Electronic mail address: jadongwong@hotmail.com Role: Role code: pointOfContact Descriptive keywords: Keywords: Keyword: nitrogenous waste Keyword: Ammonia Keyword: urea Keyword: mahi-mahi Keyword: coryphaena hippurus Keyword: transporters Keyword: waste excretion Keyword: tissue concentrations Keyword: high-energy water accommodated fractions (HEWAFs) Type: Keyword type code: theme Descriptive keywords: Keywords: Keyword: inapplicable Type: Keyword type code: place Resource constraints: title: Cite As Constraints: Use limitation: Wang, Y.. 2019. The effect of oil-exposure on urea and ammonia transporters in mahi-mahi (Coryphaena hippurus). Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/n7-q77z-bm73 Resource constraints: title: CC0 License Legal constraints: Use constraints: Restriction code: licenceUnrestricted Other constraints: This information is released under the Creative Commons license - No Rights Reserved - CC0 1.0 Universal (https://creativecommons.org/publicdomain/zero/1.0/). The person who associated a work with this deed has dedicated the work to the public domain by waiving all of his or her rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law. You can copy, modify, distribute and perform the work, even for commercial purposes, all without asking permission. Resource constraints: title: Liability and Warranty Legal constraints: Other constraints: All materials on this website are made available to GRIIDC and in turn to you "as-is." Content may only be submitted by an individual who represents and warrants that s/he has sufficient rights to be able to make the content available under a CC0 waiver. There is no warranty (expressed or implied) to these materials, their title, accuracy, non-infringement of third party rights, or fitness for any particular purpose, including the performance or results you may obtain from their use. Use these materials at your own risk. Under no circumstances shall GRIIDC be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use or the inability to use either this website or the materials available via this website. If you are dissatisfied with any website feature, content, or terms of use, your sole and exclusive remedy is to discontinue use. Aggregation Info: AggregateInformation: Aggregate Data Set Name: title: Related Publication Citation Citation: Title: Wang, Y., Pasparakis, C., Stieglitz, J. D., Benetti, D. D., & Grosell, M. (2019). The effects of Deepwater Horizon crude oil on ammonia and urea handling in mahi-mahi (Coryphaena hippurus) early life stages. Aquatic Toxicology, 216, 105294. doi:10.1016/j.aquatox.2019.105294 Date: inapplicable Aggregate Data Set Identifier: title: Related Publication DOI Identifier: Code: Anchor: xlink: https://dx.doi.org/10.1016/j.aquatox.2019.105294 title: DOI doi:10.1016/j.aquatox.2019.105294 Association Type: Association type code: crossReference Language: eng; USA Topic category: Topic category code: biota Extent: Extent: Description: Dataset contains laboratory measurements of ammonia and urea concentrations, no field sampling involved. Supplemental Information: The dataset contains 4 excel files - “Raw and Corrected Nitrogenous Waste Excretion.xlsx”, “ALS analysis.xlsx”, “qPCR data.xlsx”, and “Raw and Corrected tissue concentration.xlsx” organized under separate folders. Dataset contains the following: Tissue ammonia and urea concentrations (pmol N indiv-1) at different time points (30, 42, 48, 102 hpf); Ammonia and urea excretion rates (pmol N indiv-1 min -1) at different time points (30, 36,42, 48, 54, 72, 78, 96, 102 hpf); mRNA expression of different transporters (Rhag, Rhbg, Rhcg1, Rhcg2, Nhe2, Nhe3, UT) quantified in mahi embryos and larvae in different time points (30,36,42,48,54,78,102 hpf); and PAHs measurements of each experiment. The curve data was calculated by the software and included under each gene file. HPF means hours post fertilization. The unit for concentration is µM.
          
The dataset includes: time points, concentration (Conc., µM), standard deviation (StDev), client, project, service request, sample, lab code, sample type, date collected, date received, date extracted, date analyzed, extraction method, method, matrix, basis, units, component, dilution factor, reporting limit, detection limit, result, result notes, plate A-L, sampleS, read1, read2, avg, StDev, Conc, dilution factor, *2, volume, finale (umol individual-1), pmol N indiv-1, average, stdev, sample, ammonia concentration, urea concentration, total nitrogenous, waste concentration, total nitrogenous average, total nitrogenous stdev, total volume (ml), final umol indv-1, pmol N indiv-1, average, and stdev. The file “qPCR data.xlsx” includes a readme worksheet with headers and descriptions.
          
Note: Blank cells indicate that there is no data for those cells.|The oil from the surface (referred to as OFS) used to prepare all HEWAFs (high-energy water accommodated fraction) described herein was collected via skimming operations from surface waters by British Petroleum for testing purposes, and subsequently transferred under a chain of custody to the University of Miami (sample ID: OFS-20100719-Juniper-001 A0087M). Each HEWAF was prepared on the day of use by mixing 1g of oil per liter of filtered, UV-sterilized seawater and mixed in a Waring CB15 blender (Torrington, CT) at low speed for 30s. The mixture was immediately transferred to a glass separatory funnel and settled down for 1h. The lower 90% of the mixture was collected and used for the subsequent experiment as 100% WAF (unfiltered) that was then diluted to nominal concentrations of 2, 4 or 6% using filtered UV-sterilized seawater. Dilution solutions were mixed on a stir plate at 180 rpm for 5 mins and then aliquoted for exposures.
          
Approximately 400 embryos were raised in multiple 20L tanks and samples were collected at different time points when needed for all experiments. There are triplicates for each time points. Water quality was taken daily.
          
Gene expression changes, and ammonia and urea excretion rates were examined after the exposure of fertilized cells to HEWAF.
          
Tissue ammonia and urea concentrations were determined by ammonia assay kit (sigma AA0100) and Diacetyl Monoxime method respectively. Ammonia and urea excretion rates were determined with Micromodified Indophenol Blue and Diacetyl Monoxime methods respectively. mRNA expression raw data analysis was performed with the qPCR Miner software (Zhao and Fernald, 2005) and normalized to EF1a mRNA expression.|qPCR - Stratagene Mx3005P|Approximately 400 embryos were raised in multiple 20L tanks and samples were collected at different time points when needed for all experiments. There are triplicates for each time points. Water quality was taken daily.||Zhao, S., & Fernald, R. D. (2005). Comprehensive Algorithm for Quantitative Real-Time Polymerase Chain Reaction. Journal of Computational Biology, 12(8), 1047–1064. doi:10.1089/cmb.2005.12.1047
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Distribution info: Distribution: Distributor: Distributor: Distributor contact: Responsible party: Organisation name: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC) Contact info: Contact: Phone: Telephone: Voice: 3618253604 Address: Address: Delivery point: 6300 Ocean Drive City: Corpus Christi Administrative area: TX Postal code: 78412 Country: USA Electronic mail address: griidc@gomri.org Online Resource: Online Resource: Linkage: URL: https://data.gulfresearchinitiative.org Role: Role code: distributor Distributor format: Format: Name: xlsx Version: inapplicable File decompression technique: zip Distributor transfer options: Digital transfer options: Transfer size: 2.9536 Online: Online Resource: Linkage: URL: https://data.gulfresearchinitiative.org/data/R6.x804.000:0032 Protocol: https Name: Data Landing Page Description: GRIIDC dataset landing page Function: Online function code: information
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Metadata maintenance: Maintenance information: Maintenance and update frequency: unknown Maintenance note: This ISO metadata record was automatically generated from information provided to GRIIDC for dataset: R6.x804.000:0032 on 2021-07-29T04:53:51-05:00
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