Electro-olfactogram bicolor damselfish (Stegastes partitus)

Relationship of Effects of Cardiac Outcomes in Fish for Validation of Ecological Risk II (RECOVER II)

DOI:
10.7266/n7-48jn-7a47
 
UDI:
R6.x804.000:0023
Last Update:
May 28 2019 17:48 UTC
 
Dataset Author(s):
Schlenker, L.
Point of Contact:
Grosell, Martin
University of Miami / Rosenstiel School of Marine and Atmospheric Science
4600 Rickenbacker Causeway
Miami, Florida  33149
USA
mgrosell@rsmas.miami.edu
Funding Source:
RFP-VI
 
Extent Description:
Dataset contains laboratory measurements of olfactory responses, no field sampling involved.

Identified Submitted In-Review Available
3 3 3 3

Suggested Citation:

Schlenker, L.. 2019. Electro-olfactogram bicolor damselfish (Stegastes partitus). Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/n7-48jn-7a47

Abstract:

This dataset is derived from an experiment to determine olfactory acuity of control and oil exposed young adult bicolor damselfish (Stegastes partitus) using an electro-olfactogram (EOG) technique to measures the voltage change across the olfactory epithelium. EOG experiments were performed to directly quantify the ability of control (n=14) and oil-exposed (n=11) bicolor damselfish to detect conspecific alarm cue (CAC) as an olfactory cue at the olfactory epithelium (OE). Additional olfactory cues were also tested.

Purpose:

This dataset is derived from an experiment to determine olfactory acuity of control and oil exposed young adult bicolor damselfish (Stegastes partitus) using an electro-olfactogram (EOG) technique to measures the voltage change across the olfactory epithelium.

Theme Keywords:

olfaction, electro-olfactogram, EOG, bicolor damselfish, sublethal effects, Stegastes partitus

File Format:

csv, txt

Filename:

R6x8040000023 EOG Damselfish.zip (720.83 MB)

Dataset Downloads:

1

Electro-olfactogram bicolor damselfish (Stegastes partitus)



Identification Information
Distribution Information
Metadata Maintenance Information

Metadata: 
  File identifier: 
      R6.x804.000-0023-metadata.xml
  Language: 
      eng; USA
  Character set: 
    Character set code: 
      utf8
  Hierarchy level: 
    Scope code: 
      dataset
  Metadata author: 
    Responsible party: 
      Individual name: 
          Madeline Munoz-Bustamante
      Organisation name: 
          University of Miami / Rosenstiel School of Marine and Atmospheric Science
      Position name: 
          Program Manager
      Contact info: 
        Contact: 
          Phone: 
            Telephone: 
              Voice: 
              Facsimile: 
          Address: 
            Address: 
              Delivery point: 
                  4600 Rickenbacker Cswy
              City: 
                  Miami
              Administrative area: 
                  Florida
              Postal code: 
                  33149
              Country: 
                  USA
              Electronic mail address: 
                  mbustamante@rsmas.miami.edu
      Role: 
        Role code: 
          pointOfContact
  Date stamp: 
      2021-05-04T20:04:58+00:00
  Metadata standard name: 
      ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
  Metadata standard version: 
      ISO 19115-2:2009(E)
  Dataset URI: 
      https://data.gulfresearchinitiative.org/metadata/R6.x804.000:0023
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Identification info: Data identification: Citation: Citation: Title: Electro-olfactogram bicolor damselfish (Stegastes partitus) Alternate title: Date: Date: Date: 2019-07-01 Date type: Date type code: publication Identifier: Identifier: Code: Anchor: xlink: https://dx.doi.org/10.7266/n7-48jn-7a47 title: DOI doi:10.7266/n7-48jn-7a47 Abstract: This dataset is derived from an experiment to determine olfactory acuity of control and oil exposed young adult bicolor damselfish (Stegastes partitus) using an electro-olfactogram (EOG) technique to measures the voltage change across the olfactory epithelium. EOG experiments were performed to directly quantify the ability of control (n=14) and oil-exposed (n=11) bicolor damselfish to detect conspecific alarm cue (CAC) as an olfactory cue at the olfactory epithelium (OE). Additional olfactory cues were also tested. Purpose: This dataset is derived from an experiment to determine olfactory acuity of control and oil exposed young adult bicolor damselfish (Stegastes partitus) using an electro-olfactogram (EOG) technique to measures the voltage change across the olfactory epithelium. Status: Progress code: completed Point of contact: Responsible party: Individual name: Martin Grosell Organisation name: University of Miami / Rosenstiel School of Marine and Atmospheric Science Position name: Professor Contact info: Contact: Phone: Telephone: Voice: 3054214623 Facsimile: Address: Address: Delivery point: 4600 Rickenbacker Causeway City: Miami Administrative area: Florida Postal code: 33149 Country: USA Electronic mail address: mgrosell@rsmas.miami.edu Role: Role code: pointOfContact Point of contact: Responsible party: Individual name: Lela Schlenker Organisation name: University of Miami / Rosenstiel School of Marine and Atmospheric Science Position name: Graduate Student Contact info: Contact: Phone: Telephone: Voice: Facsimile: Address: Address: Delivery point: 4600 Rickenbacker Causeway City: Miami Administrative area: Florida Postal code: 33149 Country: USA Electronic mail address: lschlenker@miami.edu Role: Role code: pointOfContact Descriptive keywords: Keywords: Keyword: olfaction Keyword: electro-olfactogram Keyword: EOG Keyword: bicolor damselfish Keyword: sublethal effects Keyword: Stegastes partitus Type: Keyword type code: theme Descriptive keywords: Keywords: Keyword: inapplicable Type: Keyword type code: place Resource constraints: title: Cite As Constraints: Use limitation: Schlenker, L.. 2019. Electro-olfactogram bicolor damselfish (Stegastes partitus). Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/n7-48jn-7a47 Resource constraints: title: CC0 License Legal constraints: Use constraints: Restriction code: licenceUnrestricted Other constraints: This information is released under the Creative Commons license - No Rights Reserved - CC0 1.0 Universal (https://creativecommons.org/publicdomain/zero/1.0/). The person who associated a work with this deed has dedicated the work to the public domain by waiving all of his or her rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law. You can copy, modify, distribute and perform the work, even for commercial purposes, all without asking permission. Resource constraints: title: Liability and Warranty Legal constraints: Other constraints: All materials on this website are made available to GRIIDC and in turn to you "as-is." Content may only be submitted by an individual who represents and warrants that s/he has sufficient rights to be able to make the content available under a CC0 waiver. There is no warranty (expressed or implied) to these materials, their title, accuracy, non-infringement of third party rights, or fitness for any particular purpose, including the performance or results you may obtain from their use. Use these materials at your own risk. Under no circumstances shall GRIIDC be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use or the inability to use either this website or the materials available via this website. If you are dissatisfied with any website feature, content, or terms of use, your sole and exclusive remedy is to discontinue use. Aggregation Info: AggregateInformation: Aggregate Data Set Name: title: Related Publication Citation Citation: Title: Schlenker, L. S., Welch, M. J., Meredith, T. L., Mager, E. M., Lari, E., Babcock, E. A., … Grosell, M. (2019). Damsels in Distress: Oil Exposure Modifies Behavior and Olfaction in Bicolor Damselfish (Stegastes partitus). Environmental Science & Technology, 53(18), 10993–11001. doi:10.1021/acs.est.9b03915 Date: inapplicable Aggregate Data Set Identifier: title: Related Publication DOI Identifier: Code: Anchor: xlink: https://dx.doi.org/10.1021/acs.est.9b03915 title: DOI doi:10.1021/acs.est.9b03915 Association Type: Association type code: crossReference Language: eng; USA Topic category: Topic category code: biota Extent: Extent: Description: Dataset contains laboratory measurements of olfactory responses, no field sampling involved. Supplemental Information:
          Initial and final sum 50 PAH data from 6% 24-hr HEWAF exposures (Damsel EOG PAH DATA.csv); bicolor damselfish lengths, masses, treatment group, and fish ID number (Bicolor Damselfish Data.csv); voltage change across the olfactory epithelium of control and oil exposed bicolor damselfish while various olfactory cues were delivered (Labchart .txt files; e.g., file damsel 8_21_18.txt is EOG recordings from several bicolor damselfish on August 21, 2018). Dataset also includes water chemistry information such as Tank ID, Date, Time, Monitoring Period, Water Temp (Degrees C), pH (S.U.), Dissolved Oxygen (mg/L), Salinity (ppt).|Damsel EOG PAH DATA.csv: PAH Data represents the sum PAH concentration (micrograms per liter) of 50 listed PAHs as part of a static 24-hr treatment. Initial and final samples were collected for each 24-hr incubation. All oil treatments were a 6% HEWAF. Sum % refers to the percent of a particular ring class (e.g. 2-ring, 3-ring, 4-ring, and >4-ring). LABCHART TEXT FILES: Labchart text files need to be imported into Labchart (AD Instruments) to be used. File names include the date of recording (e.g. file damsel 8_21_18.txt is EOG recordings from several bicolor damselfish on August 21, 2018) which can be matched to fish information (Bicolor Damselfish Data.csv) and PAH exposure information (Damsel EOG PAH DATA.csv). Two-channels were used for recording, EOG and Filtered EOG. Filtered EOG had a 60hz filter and was used for all measurements and analysis. Electro-olfactogram (EOG) experiments were performed to directly quantify the ability of control (n=14) and oil-exposed (n=11) bicolor damselfish to detect conspecific alarm cue (CAC) as an olfactory cue at the olfactory epithelium (OE). Additional olfactory cues were also tested. These experiments were done blinded, so the researcher did not know whether the fish were oil exposed or control at the time of experiment. All EOG experiments were done in clean seawater. Not all fish included in the Labchart files provided robust data. Information on whether data was included in analysis and notes on rational if not used are included in the Bicolor Damselfish Data.csv file. Bicolor damselfish were anaesthetized with 0.2 g/L MS-222 (tricaine methanesulfonate, Western Chemical, Inc., Ferndale, WA), buffered with NaHCO3 (Sigma Aldrich, St. Louis, MO), and transferred to the submerged experimental chamber where they were ventilated with aerated culture water containing 0.1 g/L MS-222. To access the olfactory epithelium the septum that separates the anterior and posterior nares was removed and a recording electrode, a non-polarizable Ag-AgCl electrode (Warner Instruments, Hamden, CT), fitted with a 1.5 mm glass capillary tube (Warner Instruments) pulled to a fine tip and filled with 3 M potassium chloride (Sigma Aldrich) was placed on the longest lamellae of the anterior dorsal section of the OE. An identical reference electrode was placed directly posterior to the eye.     All recordings were performed blind such that the researcher did not know whether the fish was a control or oil exposed individual. The output from the recording and reference electrode was differentially amplified 1000x (DP-311, Warner Instruments), filtered (0.1Hz-0.1kHz, 50/60Hz; DP-311, Warner Instruments), digitized and filtered (60 Hz, Power Lab, AD Instruments, Dunedin, NZ), and recorded (Chart Software v. 8.1.3, AD Instruments, Colorado Springs, CO). All cues were delivered in a randomized order with each cue delivered three times. Each cue delivery was followed by the administration of a blank cue. Cue abbreviations are as follows: food=brine shrimp rinse, food.1=10% dilution of brine shrimp rinse, CAC=conspecific alarm cue, ala-1=10-1 M l-alanine, ala-2=10-2 M l-alanine, ala-3=10-3 M l-alanine, tca-3=10-3 M taurocholic acid, blank=control seawater. Brine shrimp rinse was made by soaking 0.5g of frozen brine shrimp in 200 mL of damselfish culture water for 30 minutes and filtering it through a 100 m mesh. CAC was produced by euthanizing a donor bicolor damselfish with a quick blow to the head, making six superficial cuts on the lateral sides of the fish, and rinsing the cuts with 15 mL of treatment water which was added to 20 mL of treatment water. CAC was made immediately following the establishment of successful recording for each individual and one donor fish was used for each experimental fish. Bicolor Damselfish Data.csv: Bicolor Damselfish Data.csv data includes lengths and masses of experimental and donor (sacrificed for CAC) bicolor damselfish. This dataset also includes which fish were included in analysis and treatment (oil or control). If a particular fish was excluded from analysis the reason is listed. Fish ID corresponds to comments in Labchart files. If NA is listed for a fish length or mass it was not collected by the researcher.
          
Slick oil collected from surface skimming operations during the DWH spill (sample ID: OFS-20100719-Juniper-001 A00884) was used to prepare a high-energy water accommodated fraction (HEWAF) on the day that 24-hour incubations began by mixing 1 g of oil per liter of damselfish culture water at low speed for 30 s in a Waring CB 15 blender (Torrington, CT). The blended oil and seawater mixture was immediately transferred to a glass separatory funnel, allowed to settle for 1 hour, and the lower 90% was drained and retained as the 100% water-accommodated fraction (WAF).
          
Samples for PAH analysis were collected in 250 mL amber bottles directly from tanks used for static incubations at the beginning and end of 24-hour incubations and were shipped overnight on ice to ALS Environmental (Kelso, WA) for gas chromatography/mass spectrometry—selective ion monitoring (GC/MS—SIM; based on EPA method 8270D).|A non-polarizable Ag-AgCl electrode (Warner Instruments, Hamden, CT), fitted with a 1.5 mm glass capillary tube (Warner Instruments) pulled to a fine tip and filled with 3 M potassium chloride (Sigma Aldrich) was placed on the longest lamellae of the anterior dorsal section of the OE. The output from the recording and reference electrode was differentially amplified 1000x (DP-311, Warner Instruments), filtered (0.1Hz-0.1kHz, 50/60Hz; DP-311, Warner Instruments), digitized and filtered (60 Hz, Power Lab, AD Instruments, Dunedin, NZ), and recorded (Chart Software v. 8.1.3, AD Instruments, Colorado Springs, CO).|All experiments were conducted at 27 degrees C as were 24-hour incubations. Water quality taken daily. 24-hour oil exposures were sampled immediately before and after 24-hr exposure periods. All Bicolor Damselfish were wild caught.||
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Distribution info: Distribution: Distributor: Distributor: Distributor contact: Responsible party: Organisation name: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC) Contact info: Contact: Phone: Telephone: Voice: 3618253604 Address: Address: Delivery point: 6300 Ocean Drive City: Corpus Christi Administrative area: TX Postal code: 78412 Country: USA Electronic mail address: griidc@gomri.org Online Resource: Online Resource: Linkage: URL: https://data.gulfresearchinitiative.org Role: Role code: distributor Distributor format: Format: Name: csv, txt Version: inapplicable File decompression technique: zip Distributor transfer options: Digital transfer options: Transfer size: 720.8294 Online: Online Resource: Linkage: URL: https://data.gulfresearchinitiative.org/data/R6.x804.000:0023 Protocol: https Name: Data Landing Page Description: GRIIDC dataset landing page Function: Online function code: information
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Metadata maintenance: Maintenance information: Maintenance and update frequency: unknown Maintenance note: This ISO metadata record was automatically generated from information provided to GRIIDC for dataset: R6.x804.000:0023 on 2021-07-29T04:09:56-05:00
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