Abstract:

All data were collected in a laboratory setting. The data includes images of droplets stabilized by clay platelets and by biofilms produced by the organism Alcanivorax borkumensis. The objective of the dataset is to provide information regarding natural oil dispersion, biodegradation and the production of marine snow. The data also provides information regarding the fate of oil droplets when surrounded by biofilm and the potential for oil sedimentation through oil-mineral aggregates. Detailed imaging data gives insight into bacterial sequestration by particles at the oil-water interface. In detail the following information is provided (a) three-phase contact angle measurements to understand wettability of clays at the oil-water interface (b) interfacial tension measurements as a function of clay adsorption (c) diameters of emulsion droplets (d) optimal images of emulsions droplets with biofilm (e) cryo-scanning electron microscopy of droplets colonized by bacteria (f) bacterial growth rate studies (g) oil sedimentation images (h) biodegradation quantification through gas chromatography.

Suggested Citation:

Omarova, Marzhana, John, Vijay T.. 2018. Interaction between Alcanivorax borkumensis bacterial biofilm and naturally occurring clay minerals: implications to oil sedimentation and biodegradation. Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7QC020J

Publications:

Omarova, M., Swientoniewski, L. T., Tsengam, I. K. M., Panchal, A., Yu, T., Blake, D. A., … John, V. (2018). Engineered Clays as Sustainable Oil Dispersants in the Presence of Model Hydrocarbon Degrading Bacteria: The Role of Bacterial Sequestration and Biofilm Formation. ACS Sustainable Chemistry & Engineering, 6(11), 14143–14153. doi:10.1021/acssuschemeng.8b02744

Purpose:

To provide information regarding natural oil dispersion, biodegradation, and the production of marine snow.

Data Parameters and Units:

dat.xlsx: Figure 1a: Angle (degrees), Free energy of particle detachment (deltaG/kT, unitless) for a sphere, Rod (aspect ratio of 5), Disk (aspect ratio of 5), Disk (aspect ratio of 10) Figure 1d: Carbon content (%), Contact angle (degrees), Interfacial tension (nM/m) Figure 2c: Kaolinite stabilized Anadarko crude oil emulsion droplet diameter (μm) for 1day, 1 week, and 4 weeks Figure 2d: Carbonized kaolinite stabilized Anadarko crude oil emulsion droplet diameter (μm) for 1 day, 1 week and 4 weeks Figure 5b: Relative fluorescence units (unitless) to indicate bacterial growth in samples 1-8, incubation time (hours), samples 1-4 contain oil and A. borkumensis, samples 5-8 contain carbonized kaolinite, oil, and A. borkumensis Figure 6c inset: Carbonized kaolinite stabilized Anadarko crude oil emulsion droplet diameter and A. borkumensis (μm) for 1 and 4 weeks Figure 9: Hexadecane remaining for the duration of 6 days of incubation (%) for the A. borkumensis and carbonized kaolinite plus A. borkumensis Figure S2a and Figure S2c: Kaolinite particle population (%) falling into a defined diameter range (μm) for kaolinite Figure S2b and Figure S2d: Particle population (%) falling into a defined diameter range (μm) for carbonized kaolinite Figure S3: Carbon content of modified kaolinite as estimated by thermogravimetric analysis, temperature (degrees C), weight remaining (%) Figure S4a: Kaolinite stabilized Anadarko crude oil emulsion droplet diameter and A. borkumensis (μm) for 1 day, 1week and 4 weeks Figure S5: Weight loss carbonized kaolinite plus biofilm and carbonized kaolinite as estimated by thermogravimetric analysis, temperature (degrees C), weight remaining (%) Figure S6: Gas chromatography data as responses (pA) generated by the elution time (min) for the abiotic control at the end of the six-day experiment and A. borkumensis containing sample at the end of the six-day experiment Tiff images: Figure 1b shows the three-phase contact angle measurements of natural kaolinite (angle 31 degrees) and Figure 1c shows carbonized kaolinite (0.46% carbon) and a contact angle of 89 degrees Figure 2a shows the emulsion droplet size distributions and optical images for Anadarko crude oil stabilized by kaolinite, and Figure 2b Anadarko crude oil droplets stabilized by carbonized kaolinite Figure 3 (a-d) show coverage of oil surface by particles using cryo-SEM images of kaolinite sheets on Anadarko crude oil Figure 4 (a-c) show the sheets attached face-on to the oil-water interface using cryo-SEM of a fractured Anadarko crude oil droplet Figure 5a shows the rod-shaped cells with a thick cell membrane and polyphosphate granule in a cryo-TEM of A. borkumensis Figure 6a shows the ** carbonized kaolinite stabilized emulsion droplets covered with A. borkumensis biofilm Figure 6b shows the emulsion droplets connected due to bridging by the biofilm Figure 6c shows the Anadarko crude oil droplets stabilized by carbonized kaolinite with A. borkumensis over four weeks Figures 7 (a-f) show cryo-SEM images of carbonized kaolinite droplets colonized by A. borkumensis Figure 8a-1-3 shows photos of vials showing a precipitate formed of excess particles bridged by biofilm during the carbonized kaolinite and A. borkumensis biofilm-induced precipitation Figure 8b and 8c show optical micrographs of a large dense aggregate of particles and biofilm and small droplets trapped in the biofilm-clay aggregate Figure 8d shows cryo-SEM images to demonstrate exopolymer fibers attached to particles Figure S1a shows an SEM image of dispersed kaolinite and Figure S1b carbonized kaolinite Figure S1b-inset shows a high magnification cryo-SEM of a single kaolinite particle to illustrate its plate-like shape Figure S4b shows an optical micrograph of native kaolinite and A. borkumensis biofilm stabilized Anadarko crude oil

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