Abstract:

This project investigated the effect of surfactant chemistry and concentration on the initial adhesion of Marinobacter hydrocarbonoclasticus at the interface of dodecane droplets suspended in water, investigating both the time-dependent adhesion of bacteria at the dodecane/water interface and the dependence of adhesion on droplet size and surfactant concentration. The dataset contains z-stack images (.tiff) for three different drop sizes at different time intervals, and z-stack images (.tiff) for two different drop sizes at different surfactant concentrations. The data analyzed consists of: (a) normalized drop diameter as a function of inner to outer fluid flow rates in microfluidic device; (b) number of cells as a function of time; (c) time constant of adhesion as a function of drop diameter; (d) orientation distribution of cells at dodecane/water interface for three drop sizes; (e) cell areal density as a function of surfactant concentration; (f) interfacial tension as a function of surfactant concentration; (g) cell areal density as a function of interfacial tension; and (h) zeta potential of emulsion droplets. This dataset is associated with GRIIDC dataset R5.x285.000:0008 which includes images (.bmp) for dodecane-in-water emulsions prepared using co-flow microfluidic device and pendant drop movies (.avi files) at different surfactant concentrations for the dodecane/water system. This dataset supports the publication: Dewangan, N. K., & Conrad, J. C. (2018). Adhesion of Marinobacter hydrocarbonoclasticus to Surfactant-Decorated Dodecane Droplets. Langmuir, 34(46), 14012–14021. doi:10.1021/acs.langmuir.8b02071

Suggested Citation:

Jacinta C. Conrad, Narendra K. Dewangan. 2018. Dataset for: Adhesion of Marinobacter hydrocarbonoclasticus to Surfactant-Decorated Dodecane Droplets. Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/n7-dhkx-yh57

Publications:

Dewangan, N. K., & Conrad, J. C. (2018). Adhesion of Marinobacter hydrocarbonoclasticus to Surfactant-Decorated Dodecane Droplets. Langmuir, 34(46), 14012–14021. doi:10.1021/acs.langmuir.8b02071

Purpose:

The project aimed to understand how dispersants affect the adhesion of bacteria at oil/water interfaces. We examined the effect of various surfactant chemistry and concentration on adhesion by measuring number of cells adhering at oil/water interfaces using single cell tracking algorithms. We have also investigated the effect of drop size in bacterial adhesion.

Data Parameters and Units:

Concentration (ppm), interfacial tension (mN/m), surface energy (mN/m), flow rate (ul/min), time (min), drop diameter (um), surface excess (mole/ m^2), surface area per molecule (A^2), salt concentration (M), Cell areal density (um^-2), cell volume density (ul^-1), zeta potential (mV); DOSS = dioctyl sodium succinate, CTAB = cetyltrimethylammonium bromide, DCHSS = dicyclohexyl sodium sulfosuccinate, DBSS = dibutyl sodium sulfosuccinate, CMC = critical micelle concentration. Folder Adhesion_vs_conc_DOSS_50um_SSW contains the raw data used to generate figure 7 in the paper Dewangan and Conrad (2018). File Number_of_cells_vs_surfactant_concentration.xlsx uses data included in the folder “Adhesion_vs_conc_DOSS_50um_SSW” to generate figure 9 in the paper Dewangan and Conrad (2018). File Surface_excess.xlsx uses data that is present in the folder “Processed_data_xls” which is available under GRIIDC UDI R5.x285.000:0008. This data is presented as table 1 in the paper Dewangan and Conrad (2018). File Zeta_potential_of_emulsion_droplets.xlsx contains the data used in table 2 in the paper Dewangan and Conrad (2018).

Instruments:

Confocal Microscopy

Provenance and Historical References:

Dewangan, N. K., & Conrad, J. C. (2018). Adhesion of Marinobacter hydrocarbonoclasticus to Surfactant-Decorated Dodecane Droplets. Langmuir, 34(46), 14012–14021. doi:10.1021/acs.langmuir.8b02071

Individual Files: