Abstract:

This dataset contains results from laboratory incubation of sediment resuspensions and the effects of organic matter transport and microbial metabolism in bottom waters collected from 7 sites during R/V Endeavor cruise EN527 from 2013-06-20 to 2013-06-30. Sediment cores were taken with a multi corer and experimentally resuspended in an onboard erosion chamber shortly after sampling. Following erosion experiments, the water overlying the sediment cores was filled into acrylic tanks and incubated on a roller table to simulate mildly turbulent conditions in bottom waters. The dataset contains measurements of potential enzyme activities, bacterial cell counts, and concentrations of particulate organic carbon and nitrogen.

Suggested Citation:

Kai Ziervogel. 2020. Particulate organic carbon, microbial activities and abundance from sediment incubation aboard R/V Endeavor cruise EN527 from 2013-06-20 to 2013-06-30. Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/n7-ny0s-fq42

Purpose:

Investigate the potential role of sediment resuspension on microbial activities in deep waters.

Data Parameters and Units:

The file "EN527_erosion.xlsx": Site (Station ID where sediment core was taken), core # (The number of core from one sampling event), event # (The number of the sampling event from the cruise; EN527), MUF (Methylumbelliferyl - the fluorescence tag of the substrates used to measure BG and Buty; MUF solution is used to calibrate the fluorescence signal, see 'calibration' tab), MUF factor (The slope of the regression line based on the concentration and fluorescence reading of MUF; see 'calibration' tab), MCA (Methylcoumarin - the fluorescence tag of the substrate used to measure Leu; MCA solution was used to calibrate the fluorescence signal, see 'calibration' ta), MCA factor (The slope of the regression line based on the concentration and fluorescence reading of MCA; see 'calibration' tab), SW control (sterilized bottom seawater used to control for abiotic degradation of the fluorescent labeled substrate), Slope (The slope of a regression line based on the time and fluorescence readings (FL), Rsq (The square of the Pearson product-moment correlation coefficient), corrected slope (The slope corrected with the slope from SW control incubations; note that a negative value means 'no measurable activity', nM h-1 (Unit of hydrolysis in nanomol per liter (nM) per hour (h-1)), average (The sum of replicate hydrolysis rates divided by the total number of values supplied), StDev (The standard deviation of replicate hydrolysis rates). The file "EN527_FCcounts.xlsx": Event #, Sample ID, FC count, total particles, volume run (uL), bacteria/ml, particles/ml, bacteria/particles. Please note that - Core = Sediment core used for erosion experiment; T = time point of sampling during the experiment; SSW = surrounding seawater, i.e., the water from the roller tank w/o aggregates; SL = aggregates in tank water after SSW was removed; SW control = sterilized seawater; FC = Flow cytometer. The file "EN527_POC_DW.xlsx": Date filtered (MM/DD/YYYY), Sample, mL Filtered (milliliter of water filtered through glass fibre filter (0.7-um pore size)), Initial mass (gram), Final mass (gram), DW (dry weight in gram), C (Carbon, Micrograms/filter), N (Nitrogen, Micrograms/filter), C/N ratio (Carbon and Nitrogen ratio), C (Carbon, Micrograms/L), N (Nitrogen, Micrograms/L), C/N (Carbon ratio. Please note that - Core = Sediment core used for erosion experiment; T = time point of sampling during the experiment; SSW = surrounding seawater, i.e., the water from the roller tank w/o aggregates; Slurry = aggregates in tank water after SSW was removed. The file "EN527_roller.xlsx": Site (Station ID where sediment core was taken), core # (The number of core from one sampling event), event # (The number of the sampling event from the cruise; EN527), MUF (Methylumbelliferyl - the fluorescence tag of the substrates used to measure BG and Buty; MUF solution is used to calibrate the fluorescence signal, see 'calibration' tab), MUF factor (The slope of the regression line based on the concentration and fluorescence reading of MUF; see 'calibration' tab), MCA (Methylcoumarin - the fluorescence tag of the substrate used to measure Leu; MCA solution was used to calibrate the fluorescence signal, see 'calibration' ta), MCA factor (The slope of the regression line based on the concentration and fluorescence reading of MCA; see 'calibration' tab), SW control (sterilized bottom seawater used to control for abiotic degradation of the fluorescent labeled substrate), Slope (The slope of a regression line based on the time and fluorescence readings (FL), Rsq (The square of the Pearson product-moment correlation coefficient), corrected slope (The slope corrected with the slope from SW control incubation; note that a negative value means 'no measurable activity', nM h-1 (Unit of hydrolysis in nanomol per liter (nM) per hour (h-1)), average (The sum of replicate hydrolysis rates divided by the total number of values supplied), StDev (The standard deviation of replicate hydrolysis rates). The file "EN527_sites.xlsx": SiteID, event#, Lat (Latitude, degree North), Long (Longitude, degree West), Water depth (m), Sampling date (YYYYMMDD). The dataset also includes the cruise documentation "GRIIDC_Cruise_Data_Documentation_EN527" for the R/V Endeavor cruise EN527, led by chief scientist Dr. Joseph Montoya.

Methods:

Potential enzyme activities were measured in experimental water (erosion chamber, roller tanks) by means of fluorescent labeled substrate proxies. Bacterial cells in experimental water were enumerated with a flow cytometer. Particulate organic carbon and nitrogen were analyzed on GF/F filtered particulate matter using an elemental analyzer.

Individual Files: