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Associations of metal exposure in otoliths with lesion formation in offshore, northeastern Gulf of Mexico fishes collected after the Deepwater Horizon oil spill (2011-2013)

Center for the Integrated Modeling and Analysis of Gulf Ecosystems II (C-IMAGE II)

DOI:
10.7266/N7XS5SFZ
 
UDI:
R4.x267.181:0008
Last Update:
Jun 23 2016 21:16 UTC
 
Dataset Author(s):
Peebles, Ernst
Point of Contact:
Peebles, Ernst B.
University of South Florida / College of Marine Science
140 7th Ave South
St. Petersburg, Florida  33701
USA
epeebles@marine.usf.edu
Funding Source:
RFP-IV
Data Collection Period:
2011-06-01 to 2013-08-31

Identified Submitted Review Available
3 3 3 3

Abstract:

This dataset contains annual metal concentrations for the years 2009-2011 within the otoliths of six, offshore Gulf of Mexico fishes: Red Grouper, Epinephelus morio; Red Porgy, Pagrus pagrus; Red Snapper, Lutjanus campechanus; Southern Hake, Urophycis floridana; Tilefish, Lopholatilus chamaeleonticeps; and Yellowedge Grouper, Hyporthodus flavolimbatus. Fish were collected from the northeastern Gulf of Mexico, from approximately the Louisiana Shelf to the West Florida Shelf. For each sample, a suite of 9 isotopes was measured within fish otoliths: 24Mg, 51V, 53Cr, 57Fe, 59Co, 60Ni, 63Cu, 64Zn, and 208Pb. Annual metal concentrations were determined by averaging metal concentrations corresponding to the annual increment widths in fish otoliths from approximately 2009-2011, although the exact time periods for each species are based on the timing of otolith annulus deposition. Lifetime metal concentrations are given in the dataset as the mean of entire otolith profiles (i.e. from the core to the edge of an otolith) for an individual. The dataset additionally contains information on individual fish collection location, sex, age, species, collection year, and lesion status.

Purpose:

This dataset was constructed to (1) examine patterns of short- and long-term oil-associated metal exposure within the otoliths of several offshore fish species in varying conditions, as indicated by the presence of external skin lesions, and (2) determine if there was a change in otolith oil-associated metal concentrations concurrent with the DWH oil spill. These objectives were addressed by analyzing the lifetime otolith chemistries of six offshore fish species collected in the GoM following the DWH oil spill.

Theme Keywords:

Gulf of Mexico, Metal, Otolith, Lesion, Deepwater Horizon, Red Snapper, Red Grouper, Tilefish, Yellowedge Grouper, Red Porgy, Southern Hake

File Format:

xlsx

Dataset Downloads:

1

Associations of metal exposure in otoliths with lesion formation in offshore, northeastern Gulf of Mexico fishes collected after the Deepwater Horizon oil spill (2011-2013)



Identification Information
Distribution Information
Metadata Maintenance Information

Metadata: 
  File identifier: 
      R4.x267.181-0008-metadata.xml
  Language: 
      eng; USA
  Character set: 
    Character set code: 
      utf8
  Hierarchy level: 
    Scope code: 
      dataset
  Metadata author: 
    Responsible party: 
      Individual name: 
          Richard McKenzie
      Organisation name: 
          University of South Florida / USF Library System
      Position name: 
          GIS Analyst
      Contact info: 
        Contact: 
          Phone: 
            Telephone: 
              Voice: 
                  8139742852
              Facsimile: 
          Address: 
            Address: 
              Delivery point: 
                  4202 E. Fowler Avenue LIB122
              City: 
                  Tampa
              Administrative area: 
                  Florida
              Postal code: 
                  33620
              Country: 
                  USA
              Electronic mail address: 
                  rwmcken2@usf.edu
      Role: 
        Role code: 
          pointOfContact
  Date stamp: 
      2018-08-11T13:37:21+00:00
  Metadata standard name: 
      ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
  Metadata standard version: 
      ISO 19115-2:2009(E)
  Dataset URI: 
      https://data.gulfresearchinitiative.org/metadata/R4.x267.181:0008
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Identification info: Data identification: Citation: Citation: Title: Associations of metal exposure in otoliths with lesion formation in offshore, northeastern Gulf of Mexico fishes collected after the Deepwater Horizon oil spill (2011-2013) Alternate title: Date: Date: Date: 2016-04-11 Date type: Date type code: publication Abstract: This dataset contains annual metal concentrations for the years 2009-2011 within the otoliths of six, offshore Gulf of Mexico fishes: Red Grouper, Epinephelus morio; Red Porgy, Pagrus pagrus; Red Snapper, Lutjanus campechanus; Southern Hake, Urophycis floridana; Tilefish, Lopholatilus chamaeleonticeps; and Yellowedge Grouper, Hyporthodus flavolimbatus. Fish were collected from the northeastern Gulf of Mexico, from approximately the Louisiana Shelf to the West Florida Shelf. For each sample, a suite of 9 isotopes was measured within fish otoliths: 24Mg, 51V, 53Cr, 57Fe, 59Co, 60Ni, 63Cu, 64Zn, and 208Pb. Annual metal concentrations were determined by averaging metal concentrations corresponding to the annual increment widths in fish otoliths from approximately 2009-2011, although the exact time periods for each species are based on the timing of otolith annulus deposition. Lifetime metal concentrations are given in the dataset as the mean of entire otolith profiles (i.e. from the core to the edge of an otolith) for an individual. The dataset additionally contains information on individual fish collection location, sex, age, species, collection year, and lesion status. Purpose: This dataset was constructed to (1) examine patterns of short- and long-term oil-associated metal exposure within the otoliths of several offshore fish species in varying conditions, as indicated by the presence of external skin lesions, and (2) determine if there was a change in otolith oil-associated metal concentrations concurrent with the DWH oil spill. These objectives were addressed by analyzing the lifetime otolith chemistries of six offshore fish species collected in the GoM following the DWH oil spill. Status: Progress code: completed Point of contact: Responsible party: Individual name: Ernst B. Peebles Organisation name: University of South Florida / College of Marine Science Position name: Associate Professor Contact info: Contact: Phone: Telephone: Voice: 7275533983 Facsimile: Address: Address: Delivery point: 140 7th Ave South City: St. Petersburg Administrative area: Florida Postal code: 33701 Country: USA Electronic mail address: epeebles@marine.usf.edu Role: Role code: principalInvestigator Descriptive keywords: Keywords: Keyword: Gulf of Mexico Keyword: Metal Keyword: Otolith Keyword: Lesion Keyword: Deepwater Horizon Keyword: Red Snapper Keyword: Red Grouper Keyword: Tilefish Keyword: Yellowedge Grouper Keyword: Red Porgy Keyword: Southern Hake Type: Keyword type code: theme Descriptive keywords: Keywords: Keyword: Louisiana Shelf Keyword: DeSoto Canyon Keyword: West Florida Shelf Keyword: Offshore Keyword: Gulf of Mexico Type: Keyword type code: place Language: eng; USA Topic category: Topic category code: biota Topic category: Topic category code: environment Topic category: Topic category code: oceans Extent: Extent: Geographic element: Geographic bounding box: West bound longitude: -91.917 East bound longitude: -82.28397 South bound latitude: 25.99528 North bound latitude: 30.07507 Geographic element: BoundingPolygon: Polygon: gml:MultiPoint: gml:pointMember: Point: gml:pos: 25.99528 -82.83685 gml:pointMember: Point: gml:pos: 26.12435 -82.28397 gml:pointMember: Point: gml:pos: 27.27417 -84.5165 gml:pointMember: Point: gml:pos: 27.883 -90.6 gml:pointMember: Point: gml:pos: 27.9 -91.917 gml:pointMember: Point: gml:pos: 27.96587 -84.5518 gml:pointMember: Point: gml:pos: 28 -91.917 gml:pointMember: Point: gml:pos: 28.0729 -84.4396 gml:pointMember: Point: gml:pos: 28.08678 -84.41707 gml:pointMember: Point: gml:pos: 28.08895 -84.43235 gml:pointMember: Point: gml:pos: 28.12957 -90.71408 gml:pointMember: Point: gml:pos: 28.183 -91.917 gml:pointMember: Point: gml:pos: 28.2178 -90.5845 gml:pointMember: Point: gml:pos: 28.21962 -90.64148 gml:pointMember: Point: gml:pos: 28.42705 -90.59645 gml:pointMember: Point: gml:pos: 28.58832 -89.47605 gml:pointMember: Point: gml:pos: 28.65 -91.917 gml:pointMember: Point: gml:pos: 28.7 -90 gml:pointMember: Point: gml:pos: 28.8241 -89.5074 gml:pointMember: Point: gml:pos: 28.8259 -84.9092 gml:pointMember: Point: gml:pos: 28.84793 -89.55908 gml:pointMember: Point: gml:pos: 28.8512 -89.4845 gml:pointMember: Point: gml:pos: 28.90665 -89.51313 gml:pointMember: Point: gml:pos: 28.98 -88.717 gml:pointMember: Point: gml:pos: 29.02263 -85.62068 gml:pointMember: Point: gml:pos: 29.0373 -88.7373 gml:pointMember: Point: gml:pos: 29.04628 -85.78658 gml:pointMember: Point: gml:pos: 29.08945 -89.67862 gml:pointMember: Point: gml:pos: 29.1093 -88.876 gml:pointMember: Point: gml:pos: 29.133 -88.4 gml:pointMember: Point: gml:pos: 29.15542 -88.85173 gml:pointMember: Point: gml:pos: 29.18783 -88.8863 gml:pointMember: Point: gml:pos: 29.1904 -88.3738 gml:pointMember: Point: gml:pos: 29.20202 -88.6015 gml:pointMember: Point: gml:pos: 29.20462 -85.55067 gml:pointMember: Point: gml:pos: 29.23108 -88.46688 gml:pointMember: Point: gml:pos: 29.2355 -88.5522 gml:pointMember: Point: gml:pos: 29.25433 -87.98228 gml:pointMember: Point: gml:pos: 29.285 -88.0184 gml:pointMember: Point: gml:pos: 29.28807 -87.97627 gml:pointMember: Point: gml:pos: 29.30062 -88.00915 gml:pointMember: Point: gml:pos: 29.30908 -87.99203 gml:pointMember: Point: gml:pos: 29.3319 -87.69873 gml:pointMember: Point: gml:pos: 29.4007 -87.48435 gml:pointMember: Point: gml:pos: 29.4017 -87.5017 gml:pointMember: Point: gml:pos: 29.4396 -89.69175 gml:pointMember: Point: gml:pos: 29.4525 -88.6525 gml:pointMember: Point: gml:pos: 29.46395 -87.42888 gml:pointMember: Point: gml:pos: 29.48643 -86.67123 gml:pointMember: Point: gml:pos: 29.4941 -86.6688 gml:pointMember: Point: gml:pos: 29.5263 -87.393 gml:pointMember: Point: gml:pos: 29.54817 -87.34473 gml:pointMember: Point: gml:pos: 29.63375 -88.09093 gml:pointMember: Point: gml:pos: 29.6419 -86.6586 gml:pointMember: Point: gml:pos: 29.66605 -86.72095 gml:pointMember: Point: gml:pos: 29.719 -86.619 gml:pointMember: Point: gml:pos: 29.74143 -87.18693 gml:pointMember: Point: gml:pos: 29.74837 -87.26218 gml:pointMember: Point: gml:pos: 29.7552 -87.1885 gml:pointMember: Point: gml:pos: 29.75747 -86.67655 gml:pointMember: Point: gml:pos: 29.7755 -87.2089 gml:pointMember: Point: gml:pos: 29.79072 -86.50825 gml:pointMember: Point: gml:pos: 29.80088 -87.16825 gml:pointMember: Point: gml:pos: 29.8086 -87.2252 gml:pointMember: Point: gml:pos: 29.84845 -87.2553 gml:pointMember: Point: gml:pos: 29.88348 -87.22172 gml:pointMember: Point: gml:pos: 30.07507 -86.29888 Temporal element: Temporal extent: Extent: Time period: Description: ground condition Begin date: 2011-06-01 End date: 2013-08-31 Supplemental Information: Species name: E. morio = Red Grouper, Epinephelus morio; P. pagrus = Red Porgy, Pagrus pagrus; L. campechanus = Red Snapper, Lutjanus campechanus; U. floridana = Southern Hake, Urophycis floridana; L. chamaeleonticeps = Tilefish, Lopholatilus chamaeleonticeps; and H. flavolimbatus = Yellowedge Grouper, Hyporthodus flavolimbatus. Metal concentrations: Concentrations of all of the following metals in fish otoliths in parts per million (ppm): 24Mg, 51V, 53Cr, 57Fe, 59Co, 60Ni, 63Cu, 64Zn, and 208Pb. Fish ID: Specific identification code for each individual. Collection Year: Year during which the individual fish was collected. Transect: Location of fish collection from a specific transect in the Gulf of Mexico. Depth: Depth of fish collection (m) from the Gulf of Mexico. Location: Location of fish collection from the Gulf of Mexico where 1 = Louisiana Shelf, 2 = DeSoto Canyon, 3 = West Florida Shelf. Sex: Sex of the individual fish where M = male, F = female, and UI = unidentified gender. Lesioned: Lesion status of the individual fish where Y = lesioned and N = not lesioned. Age: Age of the individual fish as determined through an examination of the otolith. Analyte Year(s): Year(s) for which averaged metal concentrations are given, either 2009, 2010, 2011, or ‘lifetime’, which represents the entire lifetime of the individual. Lat and Long: Latitude and longitude (Dec D) of fish collections from the Gulf of Mexico.|Longline surveys were conducted during the summer months (June – August) of the three years that followed the DWH oil spill (2011 – 2013) to evaluate the occurrence of diseased fish in the GoM. Fish were collected at stations along transects in the region of the DWH oil spill and off the West Florida Shelf (Fig. 1). Each station was fished for 2 h using baited demersal long lines in an 8 km string at a depth of approximately 30-390 m. Collection location and year, transect number, and lesion status (external breaks in the integument or severe skin irritation unrelated to mechanical damage) were recorded for the target fish species. Both sagittal otoliths were extracted from a subsample of the target species at the time of capture and stored for later processing. Otoliths were ablated in a sealed chamber using a PhotonMachines Analyte.193 excimer UV laser ablation (LA) system. An Agilent 7500CX Quadrupole Inductively Coupled Plasma-Mass Spectrometer (ICP-MS) was used to analyze the ablated otolith material. For each sample, a suite of 9 isotopes was measured: 24Mg, 51V, 53Cr, 57Fe, 59Co, 60Ni, 63Cu, 64Zn, and 208Pb. 43Ca was also measured for use as an internal standard. These isotopes were selected for analysis because they are less likely to have inter-element isobaric interferences during analysis and they have been measured in the Macondo crude oil from the DWH oil spill. The LA-ICP-MS instrumentation was tuned prior to data collection by ablating a National Institute of Standards (NIST) 612 glass wafer. Background element concentrations were measured prior to otolith ablation by analyzing a gas blank for 60 seconds. Samples were externally calibrated by ablating a NIST 612 glass wafer two times for 60 seconds before and after each sample transect run. Ablation of otoliths occurred along a transect with a diameter of 64.1μm extending from the primordium to the edge of the otolith along the sulcal groove at a rate of between 1 and 20μm/s. In most cases, 1-2 pre-ablation transects preceded the transect that was used for data analysis. After each otolith was analyzed, the ICP-MS was allowed to run for 60 seconds to ensure all sample material was flushed from the system. After analysis with the LA-ICP-MS, each otolith was imaged using PAX-it imaging software and aged by two independent readers. Laser ablation transect length and annual increment widths were measured using ImageJ software. The limits of detection were calculated using the Fathom toolbox for Matlab as three times the standard deviation of a sample that contains zero analyte. Limits of detection were adjusted for each sample based on ablation yield estimates. Readings below detection limits were adjusted to zero. Mean metal concentrations (ppm) were calculated from counts-per-second data using the Fathom toolbox algorithms. Annual metal concentrations were determined by averaging metal concentrations corresponding to the annual increment widths from approximately 2009-2011, although the exact time periods for each species are based on the timing of otolith annulus deposition. Lifetime metal concentrations were represented as the mean of entire otolith profiles (i.e. from the core to the edge of an otolith) for an individual. |PhotonMachines Analyte.193 excimer UV laser ablation (LA) system, Agilent 7500CX Quadrupole Inductively Coupled Plasma-Mass Spectrometer (ICP-MS), and Class 100 metal-free laminar flow bench||The limits of detection were calculated using the Fathom toolbox for Matlab as three times the standard deviation of a sample that contains zero analyte. Limits of detection were adjusted for each sample based on ablation yield estimates. Readings below detection limits were adjusted to zero.|
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Distribution info: Distribution: Distributor: Distributor: Distributor contact: Responsible party: Organisation name: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC) Contact info: Contact: Phone: Telephone: Voice: 3618253604 Address: Address: Delivery point: 6300 Ocean Drive City: Corpus Christi Administrative area: TX Postal code: 78412 Country: USA Electronic mail address: griidc@gomri.org Online Resource: Online Resource: Linkage: URL: https://data.gulfresearchinitiative.org Role: Role code: distributor Distributor format: Format: Name: xlsx Version: inapplicable File decompression technique: Distributor transfer options: Digital transfer options: Transfer size: 0.1858 Online: Online Resource: Linkage: URL: https://data.gulfresearchinitiative.org/data/R4.x267.181:0008 Protocol: https
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Metadata maintenance: Maintenance information: Maintenance and update frequency: unknown Maintenance note: This ISO metadata record was automatically generated from information provided to GRIIDC for dataset: R4.x267.181:0008 on 2018-08-18T10:42:07+00:00
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