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Species specific metabolic capacity of naphthalene and phenanthrene in three species of marine teleosts exposed to Deepwater Horizon Crude oil

Center for the Integrated Modeling and Analysis of Gulf Ecosystems II (C-IMAGE II)

DOI:
10.7266/N7057CZW
 
UDI:
R4.x267.000:0004
Last Update:
Aug 03 2017 16:19 UTC
 
Dataset Author(s):
Pulster, Erin
Point of Contact:
Murawski, Steven A
University of South Florida / College of Marine Science
140 7th Ave South
St. Petersburg, Florida  33701
USA
smurawski@usf.edu
Funding Source:
RFP-IV
 
Extent Description:
This dataset contains monohydroxylated polycyclic aromatic hydrocarbons (PAHs) metabolite measurements of fish performed in a controlled environment, no field sampling involved.

Identified Submitted Review Available
3 3 3 3

Abstract:

Select monohydroxylated polycyclic aromatic hydrocarbons (PAHs) metabolites were measured in three marine teleosts, red drum (Sciaenops ocellatus), Florida pompano (Trachinotus carolinus) and southern flounder (Paralichthys lethostigma) to evaluate species specific metabolic differences. Fish were exposed to DWH crude oil by intraperitoneal (i.p.) injections at time 0 and 48 hours with bile sampling events at 24 and 72 hours post injection. Biliary levels of monohydroxylated naphthalene and phenanthrene metabolites were confirmed and quantified using gas chromatography triple quadrapole mass spectrometry.

Purpose:

To evaluate species specific differences in PAH metabolism.

Theme Keywords:

Monohydroxylated PAHs, metabolites, naphthalene, phenanthrene, Deepwater Horizon, Sciaenops ocellatus, Trachinotus carolinus, Paralichthys lethostigma, Florida pompano, Southern flounder, Red drum

File Format:

csv

Dataset Downloads:

4

Species specific metabolic capacity of naphthalene and phenanthrene in three species of marine teleosts exposed to Deepwater Horizon Crude oil



Identification Information
Distribution Information
Metadata Maintenance Information

Metadata: 
  File identifier: 
      R4.x267.000-0004-metadata.xml
  Language: 
      eng; USA
  Character set: 
    Character set code: 
      utf8
  Hierarchy level: 
    Scope code: 
      dataset
  Metadata author: 
    Responsible party: 
      Individual name: 
          Richard McKenzie
      Organisation name: 
          University of South Florida / USF Library System
      Position name: 
          GIS Analyst
      Contact info: 
        Contact: 
          Phone: 
            Telephone: 
              Voice: 
                  8139742852
              Facsimile: 
          Address: 
            Address: 
              Delivery point: 
                  4202 E. Fowler Avenue LIB122
              City: 
                  Tampa
              Administrative area: 
                  Florida
              Postal code: 
                  33620
              Country: 
                  USA
              Electronic mail address: 
                  rwmcken2@usf.edu
      Role: 
        Role code: 
          pointOfContact
  Date stamp: 
      2017-03-01T22:04:05+00:00
  Metadata standard name: 
      ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
  Metadata standard version: 
      ISO 19115-2:2009(E)
  Dataset URI: 
      https://data.gulfresearchinitiative.org/metadata/R4.x267.000:0004
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Identification info: Data identification: Citation: Citation: Title: Species specific metabolic capacity of naphthalene and phenanthrene in three species of marine teleosts exposed to Deepwater Horizon Crude oil Alternate title: Monohydroxylated metabolites in three species of marine teleosts Date: Date: Date: 2016-11-23 Date type: Date type code: publication Abstract: Select monohydroxylated polycyclic aromatic hydrocarbons (PAHs) metabolites were measured in three marine teleosts, red drum (Sciaenops ocellatus), Florida pompano (Trachinotus carolinus) and southern flounder (Paralichthys lethostigma) to evaluate species specific metabolic differences. Fish were exposed to DWH crude oil by intraperitoneal (i.p.) injections at time 0 and 48 hours with bile sampling events at 24 and 72 hours post injection. Biliary levels of monohydroxylated naphthalene and phenanthrene metabolites were confirmed and quantified using gas chromatography triple quadrapole mass spectrometry. Purpose: To evaluate species specific differences in PAH metabolism. Status: Progress code: completed Point of contact: Responsible party: Individual name: Steven A Murawski Organisation name: University of South Florida / College of Marine Science Position name: Professor Contact info: Contact: Phone: Telephone: Voice: 7275533367 Facsimile: Address: Address: Delivery point: 140 7th Ave South City: St. Petersburg Administrative area: Florida Postal code: 33701 Country: USA Electronic mail address: smurawski@usf.edu Role: Role code: pointOfContact Point of contact: Responsible party: Individual name: Erin Pulster Organisation name: University of South Florida / College of Marine Science Position name: Graduate Student Contact info: Contact: Phone: Telephone: Voice: 9413884441390 Facsimile: Address: Address: Delivery point: 140 7th Ave South City: St. Petersburg Administrative area: Florida Postal code: 33701 Country: USA Electronic mail address: epulster@mail.usf.edu Role: Role code: pointOfContact Descriptive keywords: Keywords: Keyword: Monohydroxylated PAHs Keyword: metabolites Keyword: naphthalene Keyword: phenanthrene Keyword: Deepwater Horizon Keyword: Sciaenops ocellatus Keyword: Trachinotus carolinus Keyword: Paralichthys lethostigma Keyword: Florida pompano Keyword: Southern flounder Keyword: Red drum Type: Keyword type code: theme Descriptive keywords: Keywords: Keyword: inapplicable Type: Keyword type code: place Language: eng; USA Topic category: Topic category code: biota Extent: Extent: Description: This dataset contains monohydroxylated polycyclic aromatic hydrocarbons (PAHs) metabolite measurements of fish performed in a controlled environment, no field sampling involved. Supplemental Information: This dataset contains two files. The csv file titled “GRIIDC IP dataset 12Dec16_Pulster” contains metabolite measurements described in the abstract. The csv file titled “Biometrics_treatemt_injection_info” contains treatment times and injection volumes. The following is a brief summary describing the treatment process:
                Fish were injected at 2 ml of crude oil per kg of fish with a 2:1 (crude oil: corn oil) carrier matrix.
                There were two non-injected fish from each species sacrificed at time=0 to establish normal baseline values.
                There were four fish from each species injected with the crude/corn oil mixture and four fish from each species injected with corn oil only.
                T=0, Control (No Injections)- Sacrifice 2 fish/species, Control (Corn Oil Only) and Crude/Corn Oil- Inject 4 fish/species
                T=24 Control (Corn Oil Only) and Crude/Corn Oil- Sacrifice 2 fish/species
                T=48 Control (Corn Oil Only) and Crude/Corn Oil- Reinject 2 fish/species
                T=72 Control (Corn Oil Only) and Crude/Corn Oil- Sacrifice remaining 2 fish/species
          
The following is a list of all data parameters and units of measurement for “GRIIDC IP dataset 12Dec16_Pulster.csv”: MAP ID – Unique identifier (text); C-Image ID – Unique identifier (text); Species – Sample species (Florida Pompano, Red Drum, Southern Flounder); Treatment – treatment type (Control, Corn Oil, 24 Hour Exposure, 72 Hour Exposure, Red Drum Control, Southern Flounder Control); Weight – sample weight (kg); Length – sample length (mm); Sex – sample gender (male/female); Time – Exposure duration (hours); Monohydroxylated Metabolites (1-Naphthol, 2-Naphthol, 1-Phenanthrol, 2-Phenanthrol, 3-Phenanthrol, 4-Phenanthrol, 9-Phenanthrol) – post-injection monohydroxylated polycyclic aromatic hydrocarbons (PAHs) metabolites measurements (ng/mL);
          
The following is a list of all data parameters and units of measurement for “Biometrics_treatemt_injection_info.csv”: Species – Unique identifier (text); Fish ID – Unique identifier (text) includes species (RF=Red drum, FP=Florida pompano, or SF=Southern flounder), fish number, and if applicable code (a=no bile/insufficient volume to analyze, b=dead at 72 hrs, c=dead at 24 hrs); Weight – sample weight (kg); Length – sample length (mm); Sex – sample gender (male/female); Exposure Type (Control, Corn Oil, Crude and Corn Oil); Injection Time (T) and Treatment [T=0hr Crude Oil (mL), T=0hr Corn Oil (mL), T=48hr Crude Oil (mL), T=48hr Corn Oil (mL)];||Agilent 6890 gas chromatograph coupled to a 7010a triple quadrapole MS/MS|Exposure injections occurred at time 0 and at 48 hours, sampling events occurred at times 24 and 72 hours post injections.||
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Distribution info: Distribution: Distributor: Distributor: Distributor contact: Responsible party: Organisation name: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC) Contact info: Contact: Phone: Telephone: Voice: +1-361-825-3604 Facsimile: +1-361-825-2050 Address: Address: Delivery point: 6300 Ocean Drive Unit 5869 City: Corpus Christi Administrative area: Texas Postal code: 78412-5869 Country: USA Electronic mail address: griidc@gomri.org Online Resource: Online Resource: Linkage: URL: https://data.gulfresearchinitiative.org Role: Role code: distributor Distributor format: Format: Name: csv Version: inapplicable File decompression technique: zip Distributor transfer options: Digital transfer options: Transfer size: 0.0018 Online: Online Resource: Linkage: URL: https://data.gulfresearchinitiative.org/data/R4.x267.000:0004 Protocol: https
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Metadata maintenance: Maintenance information: Maintenance and update frequency: unknown Maintenance note: This ISO metadata record was automatically generated from information provided to GRIIDC for dataset: R4.x267.000:0004 on 2017-03-01T22:04:05+00:00
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