Fast repetition rate fluorescence responses of natural phytoplankton communities incubated for 3 days in a mesocosm experiment exposed to crude oil and chemical dispersants, July 2016

Aggregation and Degradation of Dispersants and Oil by Microbial Exopolymers (ADDOMEx)

DOI:
10.7266/N7NP22G2
 
UDI:
R4.x263.189:0009
Last Update:
May 04 2017 20:36 UTC
 
Dataset Author(s):
Laura Bretherton, Manoj Kamalanathan, Jennifer Genzer, Jessica Hillhouse, Antonietta Quigg
Point of Contact:
Schwehr, Kathleen (Kathy)
Texas A&M University at Galveston / Marine Sciences Department
P.O. Box 1675
Galveston, Texas  77553
USA
schwehrk@tamug.edu
Funding Source:
RFP-IV
 
Extent Description:
This dataset contains laboratory fluorescence experiments on phytoplankton collected from the Northern Gulf of Mexico (29.63611 N, -93.64728 W).

Identified Submitted In-Review Available
3 3 3 3

Suggested Citation:

Laura Bretherton, Manoj Kamalanathan, Jennifer Genzer, Jessica Hillhouse, Antonietta Quigg. 2017. Fast repetition rate fluorescence responses of natural phytoplankton communities incubated for 3 days in a mesocosm experiment exposed to crude oil and chemical dispersants, July 2016. Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7NP22G2

Abstract:

Fast repetition rate (FRR) fluorescence offers rapid measurement of photosynthesis in vivo, and is an effective tool for assessing stress responses in phytoplankton exposed to crude oil. Using natural phytoplankton communities collected from the Gulf of Mexico exposed to oil and the chemical dispersant Corexit, we measured fluorescence signals every 12 hours over 3 days using a Fluorescence Induction and Relaxation (FIRe) system.

Purpose:

The purpose of the coastal mesocosm experiment was to assess the response of natural microbial communities to crude oil and dispersant exposure. In order to monitor phytoplankton growth, chlorophyll fluorescence was measured using a fluorescence induction and relaxation (FIRe) system. This method allows for a multi-parameter assessment of phytoplankton photophysiology that can detect sub-lethal effects of oil and dispersants, and allow for better detection of oil and dispersant stress.

Theme Keywords:

phytoplankton, community structure, fluorescence, chlorophyll, crude oil, dispersants, marine ecology, marine microbiology

File Format:

csv

Filename:

ADDOMEX Task 3 Fast repetition rate fluorescence July 2016.csv (8.36 KB)

Dataset Downloads:

3

Fast repetition rate fluorescence responses of natural phytoplankton communities incubated for 3 days in a mesocosm experiment exposed to crude oil and chemical dispersants, July 2016



Identification Information
Distribution Information
Metadata Maintenance Information

Metadata: 
  File identifier: 
      R4.x263.189-0009-metadata.xml
  Language: 
      eng; USA
  Character set: 
    Character set code: 
      utf8
  Hierarchy level: 
    Scope code: 
      dataset
  Metadata author: 
    Responsible party: 
      Individual name: 
          Antonietta Quigg
      Organisation name: 
          Texas A&M University at Galveston / Marine Biology Department
      Position name: 
          Professor
      Contact info: 
        Contact: 
          Phone: 
            Telephone: 
              Voice: 
                  4097404990
              Facsimile: 
          Address: 
            Address: 
              Delivery point: 
                  P.O. Box 1675
              City: 
                  Galveston
              Administrative area: 
                  Texas
              Postal code: 
                  77553
              Country: 
                  USA
              Electronic mail address: 
                  quigga@tamug.edu
      Role: 
        Role code: 
          pointOfContact
  Date stamp: 
      2018-08-11T13:35:57+00:00
  Metadata standard name: 
      ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
  Metadata standard version: 
      ISO 19115-2:2009(E)
  Dataset URI: 
      https://data.gulfresearchinitiative.org/metadata/R4.x263.189:0009
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Identification info: Data identification: Citation: Citation: Title: Fast repetition rate fluorescence responses of natural phytoplankton communities incubated for 3 days in a mesocosm experiment exposed to crude oil and chemical dispersants, July 2016 Alternate title: ADDOMEx Task 3 Fast repetition rate fluorescence Date: Date: Date: 2017-05-04 Date type: Date type code: publication Identifier: Identifier: Code: Anchor: xlink: https://dx.doi.org/10.7266/N7NP22G2 title: DOI doi:10.7266/N7NP22G2 Abstract: Fast repetition rate (FRR) fluorescence offers rapid measurement of photosynthesis in vivo, and is an effective tool for assessing stress responses in phytoplankton exposed to crude oil. Using natural phytoplankton communities collected from the Gulf of Mexico exposed to oil and the chemical dispersant Corexit, we measured fluorescence signals every 12 hours over 3 days using a Fluorescence Induction and Relaxation (FIRe) system. Purpose: The purpose of the coastal mesocosm experiment was to assess the response of natural microbial communities to crude oil and dispersant exposure. In order to monitor phytoplankton growth, chlorophyll fluorescence was measured using a fluorescence induction and relaxation (FIRe) system. This method allows for a multi-parameter assessment of phytoplankton photophysiology that can detect sub-lethal effects of oil and dispersants, and allow for better detection of oil and dispersant stress. Status: Progress code: completed Point of contact: Responsible party: Individual name: Kathleen (Kathy) Schwehr Organisation name: Texas A&M University at Galveston / Marine Sciences Department Position name: Associate Research Scientist Contact info: Contact: Phone: Telephone: Voice: 4097404452 Facsimile: Address: Address: Delivery point: P.O. Box 1675 City: Galveston Administrative area: Texas Postal code: 77553 Country: USA Electronic mail address: schwehrk@tamug.edu Role: Role code: pointOfContact Point of contact: Responsible party: Individual name: Antonietta Quigg Organisation name: Texas A&M University at Galveston / Marine Biology Department Position name: Professor Contact info: Contact: Phone: Telephone: Voice: 4097404990 Facsimile: Address: Address: Delivery point: P.O. Box 1675 City: Galveston Administrative area: Texas Postal code: 77553 Country: USA Electronic mail address: quigga@tamug.edu Role: Role code: principalInvestigator Descriptive keywords: Keywords: Keyword: phytoplankton Keyword: community structure Keyword: fluorescence Keyword: chlorophyll Keyword: crude oil Keyword: dispersants Keyword: marine ecology Keyword: marine microbiology Type: Keyword type code: theme Descriptive keywords: Keywords: Keyword: inapplicable Type: Keyword type code: place Resource constraints: title: Cite As Constraints: Use limitation: Laura Bretherton, Manoj Kamalanathan, Jennifer Genzer, Jessica Hillhouse, Antonietta Quigg. 2017. Fast repetition rate fluorescence responses of natural phytoplankton communities incubated for 3 days in a mesocosm experiment exposed to crude oil and chemical dispersants, July 2016. Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7NP22G2 Resource constraints: title: CC0 License Legal constraints: Use constraints: Restriction code: licenceUnrestricted Other constraints: This information is released under the Creative Commons license - No Rights Reserved - CC0 1.0 Universal (https://creativecommons.org/publicdomain/zero/1.0/). The person who associated a work with this deed has dedicated the work to the public domain by waiving all of his or her rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law. You can copy, modify, distribute and perform the work, even for commercial purposes, all without asking permission. Resource constraints: title: Liability and Warranty Legal constraints: Other constraints: All materials on this website are made available to GRIIDC and in turn to you "as-is." Content may only be submitted by an individual who represents and warrants that s/he has sufficient rights to be able to make the content available under a CC0 waiver. There is no warranty (expressed or implied) to these materials, their title, accuracy, non-infringement of third party rights, or fitness for any particular purpose, including the performance or results you may obtain from their use. Use these materials at your own risk. Under no circumstances shall GRIIDC be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use or the inability to use either this website or the materials available via this website. If you are dissatisfied with any website feature, content, or terms of use, your sole and exclusive remedy is to discontinue use. Aggregation Info: AggregateInformation: Aggregate Data Set Name: title: Related Publication Citation Citation: Title: Bretherton, L., Kamalanathan, M., Genzer, J., Hillhouse, J., Setta, S., Liang, Y., … Quigg, A. (2019). Response of natural phytoplankton communities exposed to crude oil and chemical dispersants during a mesocosm experiment. Aquatic Toxicology, 206, 43–53. doi:10.1016/j.aquatox.2018.11.004 Date: inapplicable Aggregate Data Set Identifier: title: Related Publication DOI Identifier: Code: Anchor: xlink: https://dx.doi.org/10.1016/j.aquatox.2018.11.004 title: DOI doi:10.1016/j.aquatox.2018.11.004 Association Type: Association type code: crossReference Language: eng; USA Topic category: Topic category code: biota Topic category: Topic category code: environment Topic category: Topic category code: oceans Extent: Extent: Description: This dataset contains laboratory fluorescence experiments on phytoplankton collected from the Northern Gulf of Mexico (29.63611 N, -93.64728 W). Supplemental Information: Date (Month/Day/Year), Time Point (hours 0, 12, 24, 36, 48, 60, 72), Treatment (Control, WAF=water accommodated fraction, CEWAF=chemically enhanced water accommodated fraction, DCEWAF=diluted chemically enhanced water accommodated fraction), Replicate (A, B, C, FSW=filtered seawater, WAF, CEWAF, DCEWAF), Fo (baseline fluorescence, dimensionless), Fm (maximum fluorescence, dimensionless), Fv/Fm (photosynthetic quantum yield, dimensionless), sigma (functional absorption cross-section of photosystem II, Å^2/quanta), p (connectivity factor, dimensionless), tau1 (electron turnover time on acceptor side of photosystem II, µs), tau2 (electron turnover time between photosystem II and photosystem I, µs), chl-a (chlorophyll concentration by fluorescence, dimensionless)|Natural seawater was collected from a coastal buoy in the Gulf of Mexico and brought back to Texas A&M University at Galveston for manipulation. Treatments were prepared in specialized glass tanks called WAF makers using the natural seawater. WAF treatments were prepared by adding Macondo crude oil to a target concentration of 1mg L-1, and CEWAF treatments were made with a stock solution containing a 20:1 ratio of oil:Corexit. One third of the CEWAF was then diluted until the crude oil concentration was consistent with the WAF to make the DCEWAF treatments. Control treatments were not exposed to either oil or Corexit. The experiments were then set up in 100L glass tanks staged in a room fitted with fluorescent bulbs. All treatments were grown in triplicate with true biological replication. A 4mL sample was retrieved from each tank every 12h over the course of 3 days. The samples were left to dark-acclimate for ~10 minutes before being transferred into the Fluorescence Induction and Relaxation (FIRe) system (Satlantic, Halifax, Canada). Parameters were collected from both single turnover (ST) and multiple turnover (MT) curves, using 60 acquisitions per sample to reduce noise. The sample was then recovered from the FIRe to measure the relative fluorescence intensity of chlorophyll-a using a Turner fluorometer (Turner Designs, San Jose, USA). |All data except chlorophyll concentration were collected using a Fluorescence Induction and Relaxation (FIRe) System (Satlantic, Halifax, Canada). Chlorophyll was measured with a 10AU Field Fluorometer (Turner Designs, San Jose, USA).|4mL every 12h for 72h.|N/D = below detectable limits|
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Distribution info: Distribution: Distributor: Distributor: Distributor contact: Responsible party: Organisation name: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC) Contact info: Contact: Phone: Telephone: Voice: 3618253604 Address: Address: Delivery point: 6300 Ocean Drive City: Corpus Christi Administrative area: TX Postal code: 78412 Country: USA Electronic mail address: griidc@gomri.org Online Resource: Online Resource: Linkage: URL: https://data.gulfresearchinitiative.org Role: Role code: distributor Distributor format: Format: Name: csv Version: inapplicable File decompression technique: Distributor transfer options: Digital transfer options: Transfer size: 0.0084 Online: Online Resource: Linkage: URL: https://data.gulfresearchinitiative.org/data/R4.x263.189:0009 Protocol: https Name: Data Landing Page Description: GRIIDC dataset landing page Function: Online function code: information
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Metadata maintenance: Maintenance information: Maintenance and update frequency: unknown Maintenance note: This ISO metadata record was automatically generated from information provided to GRIIDC for dataset: R4.x263.189:0009 on 2020-09-24T08:56:18-05:00
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