Abstract:

Mesocosm experiments were performed as part of the ADDOMEx GOMRI funded program with seawater collected from the Northern Gulf of Mexico (NGOM) and “seeded” with microbial populations collected from coastal Texas (in Galveston Bay). Of the treatments, negative electrospray ionization mass spectrometry coupled to Fourier transform ion cyclotron resonance mass spectrometry (ESI-FTICR-MS) spectra were collected for (A) pyridine extract of marine snow collected from control mesocosm tank (B) pyridine extract of marine snow collected from WAF mesocosm tank and (C) bulk DOM (dissolved organic matter) from control mesocosm tank. The data contained in this dataset represents the raw m/z and intensity data obtained directly from the instrument and the processed Excel files used for the analysis. This data was used to create the figures in the original manuscript.

Suggested Citation:

Obeid, Wassim; Hatcher, Pat. 2016. Raw and processed negative electrospray ionization coupled to Fourier transform ion cyclotron resonance mass spectrometry (ESI-FTICR-MS) data for mesocosm experiment samples collected from bulk DOM tank, control tank marine snow, and WAF tank marine snow.. Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N72805NC

Publications:

Quigg, A., Passow, U., Chin, W.-C., Xu, C., Doyle, S., Bretherton, L., … Santschi, P. H. (2016). The role of microbial exopolymers in determining the fate of oil and chemical dispersants in the ocean. Limnology and Oceanography Letters. doi:10.1002/lol2.10030

Purpose:

To investigate molecular level differences between marine snow extracts from a control tank and a WAF tank.

Data Parameters and Units:

for the text files: mass lists of m/z values (m= mass; z=charge of the ion) and their peak intensities (arbitrary units) for the cvs files: type indicates the chemical molecular formula; m/z is the mass to ionic charge ratio; PeakInt is peak intensity in arbitrary units; C= carbon abundance; H-1 =proton abundance; H= hydrogen abundance; N=nitrogen abundance; O=oxygen abundance; S=sulfur abundance; P=phosphorus abundance; exact mass =molucular weight

Methods:

Mesocosm methods – COASTal water with coastal microbial concentrate, COAST. Twelve 100L mesocosm tanks were filled with Gulf of Mexico seawater collected 5 miles off shore from Galveston (TX) that had been pre-treated with a charcoal filter to remove large particles and debris. Plankton (<63 µm) were collected using a net and transferred into polycarbonate bottles. This concentrated plankton mass was introduced to the tanks (2 L to each final volume 83 L) immediately prior to starting the experiments. Four treatments were prepared in triplicate. Control tanks were filled with seawater and plankton mixture. Water accommodated fraction (WAF) of oil was prepared by mixing 25 mL (5 ml ~ every 30 min for 2.5 hrs) of Macondo surrogate oil into 130 L of seawater then mixing for 12 to 24 hrs. (Knap et al. 1986; Knap et al. 2016 in preparation). The WAF was then introduced into the WAF mesocosm tanks and filled to 87 L and mixed. From these WAF tanks 6 L was removed for other experiments and analyses (2L for roller tables, 2 L dark/light, 4 L hydrocarbon analyses). In order to make chemically enhanced water accommodated fraction (CEWAF), Corexit was mixed with oil in a ratio of 1:20 and 25 mL of this mixture (5 ml every 30 min for 2.5 hrs) of surrogate oil plus Corexit was added to 130 L of seawater which was mixed for 8 to 24 hrs prior to being loaded into the mesocosm tanks. The CEWAF was then introduced into the CEWAF mesocosm tanks and filled to 96 L and mixed. From these CEWAF tanks 15 L was removed for other experiments and analyses (9 L for the DCEWAF mesocosms, 2L for roller tables, 2 L dark/light, 4 L hydrocarbon analyses). Diluted CEWAF (DCEWAF) was prepared by mixing 9 L of CEWAF with 78 L of the original seawater for a total volume of 87 L. From these DCEWAF tanks 6 L was removed for other experiments and analyses (2L for roller tables, 2 L dark/light, 4 L hydrocarbon analyses). The estimated oil equivalents (EOE) were determined using Macondo surrogate oil as the calibration standard (Wade et al. 2011) for the fluorescence analyses (Horiba Scientific Aqualog Fluorometer). The EOE mean concentration of the three mesocosms for the control, WAF, DCEWAF and CEWAF at the start of the experiments were 0 mg/L, 0.26 mg/L, 2.74 mg/L and 41.5 mg/L, respectively. The EOE mean concentration of the three mesocosms for the in the control, WAF, DCEWAF and CEWAF after 72 hours were 0 mg/L , 0.06 mg/L, 1.03. and 17.3 mg/L, respectively. Knap, A. H., T. D. Sleeter, R. E. Dodge, S. C. Wyers, H. R. Frith, and S. R. Smith. 1983. The effects of oil spills and dispersants use on corals: A review and multidisciplinary experimental approach. Oil and Petrochemical Pollution 1: 157–169. Wade, T.L., Sweet S.T., Sericano, J.L., N.L. Guinasso Jr., Diercks, A.-R., Highsmith, R.C., Asper, V.L., Joung, D., Shiller, A.M., Lohrenz, S.E. and Joye, S.B. 2011, Analyses of Water Samples from the Deepwater Horizon Oil Spill: Documentation of the Sub-Surface Plume. in Monitoring and Modeling the Deepwater Horizon Oil Spill: A Record-Breaking Enterprise, Geophysical Monograph Series, vol. 195, edited by Y. Liu et al., pp. 77–82, AGU, Washington, D. C., doi:10.1029/2011GM001103

Instruments:

Apollo II ESI ion source coupled to a Bruker Daltonics 12 Tesla Apex Qe ESI FTICR-MS

Individual Files: