Percent relative abundance of the eukaryotic microbial community in the GOMCOAST (Gulf of Mexico, Coastal Water) mesocosm from 8 micron polycarbonate filters
Identification InformationDistribution InformationMetadata Maintenance Information
Metadata:
File identifier:
R4.x263.000-0042-metadata.xml
Language:
eng; USA
Character set:
Character set code:
utf8
Hierarchy level:
Scope code:
dataset
Metadata author:
Responsible party:
Individual name:
Kathleen (Kathy) Schwehr
Organisation name:
Texas A&M University at Galveston / Marine Sciences Department
Position name:
Associate Research Scientist
Contact info:
Contact:
Phone:
Telephone:
Voice:
4097404452
Facsimile:
Address:
Address:
Delivery point:
P.O. Box 1675
City:
Galveston
Administrative area:
Texas
Postal code:
77553
Country:
USA
Electronic mail address:
schwehrk@tamug.edu
Role:
Role code:
pointOfContact
Date stamp:
2018-08-27T21:03:38+00:00
Metadata standard name:
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
Metadata standard version:
ISO 19115-2:2009(E)
Dataset URI:
https://data.gulfresearchinitiative.org/metadata/R4.x263.000:0042
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Identification info:
Data identification:
Citation:
Citation:
Title:
Percent relative abundance of the eukaryotic microbial community in the GOMCOAST (Gulf of Mexico, Coastal
Water) mesocosm from 8 micron polycarbonate filters
Alternate title:
Relative abundance of eukaryotic microbes as part of the GOMCOAST mesocosm experiment
Date:
Date:
Date:
2018-08-27
Date type:
Date type code:
publication
Identifier:
Identifier:
Code:
Anchor: xlink: https://dx.doi.org/10.7266/N7JD4V8Z title: DOI
doi:10.7266/N7JD4V8Z
Abstract:
This dataset provides an estimate of the relative abundance of active microbial eukaryotes within and across 3
treatments (oil, oil and Corexit, and a dilute oil and Corexit treatment) in the GOMCOAST mesocosm
experiment based on 18S RNA sequences from RNAseq data. The data reflects the relative presence, activity
and detectability of eukaryotic species captured on an 8 micron polycarbonate filter.
Purpose:
To determine how oil and oil and dispersant mixtures influence eukaryotic microbial community composition.
Status:
Progress code:
completed
Point of contact:
Responsible party:
Individual name:
Zoe Finkel
Organisation name:
Mount Allison University / Department of Geography and Environment
Position name:
Professor
Contact info:
Contact:
Phone:
Telephone:
Voice:
5063642615
Facsimile:
Address:
Address:
Delivery point:
144 Main St.
City:
Sackville
Administrative area:
New Brunswick
Postal code:
E4L 1A8
Country:
CAN
Electronic mail address:
zfinkel@mta.ca
Role:
Role code:
pointOfContact
Descriptive keywords:
Keywords:
Keyword:
Eukaryotic community composition analysis
Keyword:
18S
Keyword:
RNAseq
Keyword:
eukaryotes
Keyword:
percent relative abundance
Keyword:
mesocosm
Type:
Keyword type code:
theme
Descriptive keywords:
Keywords:
Keyword:
inapplicable
Type:
Keyword type code:
place
Resource constraints: title: Cite As
Constraints:
Use limitation:
Zoe V. Finkel, Andrew J. Irwin, Yue Liang, Chris M. Brown, Michaël Bradet-Legris, Laura Bretherton,
Antonietta Quigg. 2018. Percent relative abundance of the eukaryotic microbial community in the GOMCOAST
(Gulf of Mexico, Coastal Water) mesocosm from 8 micron polycarbonate filters. Distributed by: Gulf of
Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas
A&M University–Corpus Christi. doi:10.7266/N7JD4V8Z
Resource constraints: title: CC0 License
Legal constraints:
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licenceUnrestricted
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This information is released under the Creative Commons license - No Rights Reserved - CC0 1.0 Universal
(https://creativecommons.org/publicdomain/zero/1.0/). The person who associated a work with this deed
has dedicated the work to the public domain by waiving all of his or her rights to the work worldwide
under copyright law, including all related and neighboring rights, to the extent allowed by law. You can
copy, modify, distribute and perform the work, even for commercial purposes, all without asking
permission.
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Aggregation Info:
AggregateInformation:
Aggregate Data Set Name: title: Related Publication Citation
Citation:
Title:
Bretherton, L., Kamalanathan, M., Genzer, J., Hillhouse, J., Setta, S., Liang, Y., … Quigg, A. (2019).
Response of natural phytoplankton communities exposed to crude oil and chemical dispersants during a
mesocosm experiment. Aquatic Toxicology, 206, 43–53. doi:10.1016/j.aquatox.2018.11.004
Date:
inapplicable
Aggregate Data Set Identifier: title: Related Publication DOI
Identifier:
Code:
Anchor: xlink: https://dx.doi.org/10.1016/j.aquatox.2018.11.004 title: DOI
doi:10.1016/j.aquatox.2018.11.004
Association Type:
Association type code:
crossReference
Language:
eng; USA
Topic category:
Topic category code:
oceans
Topic category:
Topic category code:
biota
Topic category:
Topic category code:
environment
Extent:
Extent:
Description:
Dataset contains laboratory measurements of percent relative abundance of eukaryotes, no field sampling
involved.
Supplemental Information:
Raw data_GOMCOAST-taxonomic-composition-data-analysis.csv
KPC: Kingdom, Phylum, or Class, derived from columns 2-4 and chosen to produce a useful decomposition of total
diversity in a small number of categories
Kingdom: The Kingdom of the taxon, from World Registry of Marine Species (WoRMS, marinespecies.org)
Phylum: The phylum of the taxon from WoRMS
Class: The class of the taxon from WoRMS
Genus: The genus ot the taxon from WoRMS
target_id: The ID from the silva SSU non-redundant database version 123
qval.WAF: The multiple-comparisons corrected p-value for the hypothesis that relative abundance is the same in
control and WAF treatments
b.WAF: The log2 fold change in relative abundance between WAF and control (> 0 means higher relative abundance
in WAF)
sig.WAF: A flag of 0 or 1 to indicate if there is a change due to the treatment (1= change); used to produce
the summary table
qval.dCEWAF: The multiple-comparisons corrected p-value for the hypothesis that relative abundance is the same
in control and dilute CEWAF treatments
b.dCEWAF: The log2 fold change in relative abundance between dilute CEWAF and control (> 0 means higher
relative abundance in WAF)
sig.dCEWAF: A flag of 0 or 1 to indicate if there is a change due to the treatment (1= change); used to
produce the summary table
qval.CEWAF: The multiple-comparisons corrected p-value for the hypothesis that relative abundance is the same
in control and CEWAF treatments
b.CEWAF: The log2 fold change in relative abundance between CEWAF and control (> 0 means higher relative
abundance in WAF)
sig.CEWAF: A flag of 0 or 1 to indicate if there is a change due to the treatment (1= change); used to produce
the summary table
control: The estimated relative abundance (as %) of this taxon from kallisto in the control
WAF: The estimated relative abundance of this taxon from kallisto in the WAF treatment
DCEWAF: The estimated relative abundance of this taxon from kallisto in the dilute CEWAF treatment
CEWAF: The estimated relative abundance of this taxon from kallisto in the CEWAF treatment
Summary_GOMCOAST-taxonomic-composition-data-analysis.csv
Number with significant differential abundance relative to control: Category, n, WAF, dCEWAF, CEWAF
Proportion of total abundance (%): Control, WAF, DCEWAF, CEWAF
Kingdom-Phylum-Class: The taxonomic affiliation derived from column 1 of the raw data table
Count of Genus: The number of taxa (rows) aggregated from the raw data table to produce this summary
Sum of sig.WAF: The number of these taxa with significant differential abundance in WAF relative to control
Sum of sig.dCEWAF: The number of these taxa with significant differential abundance in dilute CEWAF relative
to control
Sum of sig.CEWAF: The number of these taxa with significant differential abundance in CEWAF relative to control
Sum of control: The proportion (as a %) of total abundance attributed to this taxon in the control
Sum of WAF: The proportion (as a %) of total abundance attributed to this taxon in the WAF treatment
Sum of DCEWAF: The proportion (as a %) of total abundance attributed to this taxon in the dilute CEWAF treatment
Sum of CEWAF: The proportion (as a %) of total abundance attributed to this taxon in the CEWAF
treatment|Twelve 100L mesocosm tanks were filled with Gulf of Mexico seawater collected from the Texas
coastline, near TABS buoy R (29° 38.1000'N, 93° 38.5020'W) which is located ~100 miles away from Galveston
(TX). Four treatments were prepared in triplicate. Control tanks were filled with seawater. Water
accommodated fraction (WAF) of oil was prepared by mixing 25 mL (5 ml ~ every 30 min for 2.5 hrs) of Macondo
surrogate oil into 130 L of seawater. Mixing ended 24 hrs. after the initial oil addition (Knap et al. 1986;
Wade et al. 2017in preparation). The WAF was then introduced into the WAF mesocosm tanks and filled to 87 L
and mixed. From these WAF tanks 6 L was removed for other experiments and analyses (2 L dark/light, 4 L
hydrocarbon analyses). In order to make chemically enhanced water accommodated fraction (CEWAF), Corexit was
mixed with oil in a ratio of 1:20 and 25 mL of this mixture (5 ml every 30 min for 2.5 hrs) of surrogate oil
plus Corexit was added to 130 L of seawater. Mixing ended 24 hrs after the initial oil addition. The CEWAF
was then introduced into the CEWAF mesocosm tanks and filled to 96 L and mixed. From these CEWAF tanks 13 L
was removed for other experiments and analyses (7 L for the DCEWAF mesocosms, 2 L dark/light, 4 L
hydrocarbon analyses). Diluted CEWAF (DCEWAF) was prepared by mixing 9 L of CEWAF with 78 L of the original
seawater for a total volume of 87 L. From these DCEWAF tanks 6 L was removed for other experiments and
analyses (2 L dark/light, 4 L hydrocarbon analyses). To the water in the 12 mesocosms, nutrients were added
(final concentration f/20) and the tanks stirred. Banks of lights were placed behind each of the glass
mesocosm tanks and a 12:12 light/dark cycle employed. Sampling commenced and defined as time zero. The
estimated oil equivalents (EOE) were determined using Macondo surrogate oil as the calibration standard
(Wade et al. 2011) for the fluorescence analyses (Horiba Scientific Aqualog Fluorometer). The EOE mean
concentration of the three mesocosms for the control, WAF, DCEWAF and CEWAF at the start of the experiments
were 0 mg/L, 0.26 mg/L, 2.74 mg/L and 41.5 mg/L, respectively. The EOE mean concentration of the three
mesocosms for the in the control, WAF, DCEWAF and CEWAF after 72 hours were 0 mg/L , 0.06 mg/L, 1.03. and
17.3 mg/L, respectively.
The estimate of the relative abundance of active microbial eukaryotes within and across treatments is based on
18S RNA sequences from RNAseq data and reflects the relative presence and activity and detectability of each
species. RNA was harvested from each of the 12 mesocosm tanks (3 replicate tanks for each treatment:
control, WAF, CEWAF, DECWAF) 72 hours after the initiation of the experiment. Several hundred mL (250 to
4000 mL) were rapidly and gently filtered onto two 47mm, 8 m polycarbonate filters. We limited the total
filtration time to 20 minutes. It took more time to filter water from the CEWAF tanks, and therefore less
volume was filtered from this treatment. The filters and denaturation solution (Ambion Simply RNA) was added
to Y-matrix bead beater tubes (MoBio). The samples were lysed using a SuperFastprep2 bead beater (30 seconds
at the maximum setting) and immediately stored in a -80°C freezer. RNA was extracted by exposing samples
immediately after thawing to 2 additional 30 second rounds in the SuperFastprep2 bead beater. The Ambion
Total RNA kit (ThermoFisher AM1910) was used to extract RNA followed by DNA removal with the Ambion Turbo
DNAfree kit (ThermoFisher AM1907) as per manufacturer instructions.
RNA was sequenced as 125 base pair paired-end reads using Illumina HiSeq 250 RNAseq by Genome Quebec. PolyA
selection was used to remove the majority of the rRNA using the NEBNext Poly(A) mRNA magnetic isolation
module kit from New England Biolabs. Approximately 2-10% of the original rRNA sequences pass through this
step are sequenced (Abernathy and Overturf 2016). Trimmomatic was used to remove Illumina adapters and low
quality bases were identified using Phred scores (Bolger, Lohse et al. 2014). Kraken and the Silva SSU rRNA
Ref NR 99 database (release 123) were used to filter reads matching 18S sequences (Quast, Pruesse et al.
2013, Wood and Salzberg 2014). Kallisto and Sleuth were used to match, count and perform differential
expression and relative abundance analysis for reads against the Silva 18S database (Bray, Pimentel et al.
2016, Pimentel, Bray et al. 2017). Three replicate mesocosms were sequenced for the control and WAF
treatment, but following quality control only two replicates were available for the DCEWAF treatment and one
replicate for the CEWAF treatment.
Each 18S sequence in the Silva database is identified with an accession code and a hierarchical taxonomic
identification. All non-Eukaryota, Metazoa and Embryophyta sequences were removed from the analyses. The
taxonomic identification in Silva is hierarchical but the identifications do not all have the same number of
levels in the hierarchy so the phylum, order, or class, for example, are not readily extracted. Where
possible we use the taxonomic hierarchy used by WoRMS in 2016/17. The genus and species name for each taxon
in the Silva database (the last entry in the hierarchy) was used to search the World Registry of Marine
Species (WoRMS, marinespecies.org). A variety of secondary sources were used to identify the taxonomic
hierarchy for taxa without a match in WoRMS including the Global Names Index (GNI), the Pan-European Species
Infrastructure (PESI), the Paleobiology database (Paleo), and the taxonomic hierarchy used by
Wikipedia/Wikispecies. An automatic search for all taxa was performed using lifewatch.be on 2016 December 31
using WoRMS, GNI, PESI, and Paleo.||One sampling point was taken and analysed as described in the methods
above.|There is no error quantified for the individual point estimates of relative abundance across
treatments. The average relative abundance was estimated from replicate samples when available as described
in the methods.|The original raw sequence data from the Illumina sequencer have been deposited at NCBI’s
Short read archive (SRA) under BioProject PRJNA338185. The data are archived as BioSample SAMN08118040 and
are accessible at http://www.ncbi.nlm.nih.gov/biosample/8118040
Jason Abernathy
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Distribution info:
Distribution:
Distributor:
Distributor:
Distributor contact:
Responsible party:
Organisation name:
Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC)
Contact info:
Contact:
Phone:
Telephone:
Voice:
3618253604
Address:
Address:
Delivery point:
6300 Ocean Drive
City:
Corpus Christi
Administrative area:
TX
Postal code:
78412
Country:
USA
Electronic mail address:
griidc@gomri.org
Online Resource:
Online Resource:
Linkage:
URL:
https://data.gulfresearchinitiative.org
Role:
Role code:
distributor
Distributor format:
Format:
Name:
csv, txt
Version:
inapplicable
File decompression technique:
7z
Distributor transfer options:
Digital transfer options:
Transfer size:
0.4218
Online:
Online Resource:
Linkage:
URL:
https://data.gulfresearchinitiative.org/data/R4.x263.000:0042
Protocol:
https
Name:
Data Landing Page
Description:
GRIIDC dataset landing page
Function:
Online function code:
information
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Metadata maintenance:
Maintenance information:
Maintenance and update frequency:
unknown
Maintenance note:
This ISO metadata record was automatically generated from information provided to GRIIDC for dataset:
R4.x263.000:0042 on 2021-03-02T02:08:52-06:00
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<gco:CharacterString>Raw data_GOMCOAST-taxonomic-composition-data-analysis.csv
KPC: Kingdom, Phylum, or Class, derived from columns 2-4 and chosen to produce a useful decomposition of total diversity in a small number of categories
Kingdom: The Kingdom of the taxon, from World Registry of Marine Species (WoRMS, marinespecies.org)
Phylum: The phylum of the taxon from WoRMS
Class: The class of the taxon from WoRMS
Genus: The genus ot the taxon from WoRMS
target_id: The ID from the silva SSU non-redundant database version 123
qval.WAF: The multiple-comparisons corrected p-value for the hypothesis that relative abundance is the same in control and WAF treatments
b.WAF: The log2 fold change in relative abundance between WAF and control (> 0 means higher relative abundance in WAF)
sig.WAF: A flag of 0 or 1 to indicate if there is a change due to the treatment (1= change); used to produce the summary table
qval.dCEWAF: The multiple-comparisons corrected p-value for the hypothesis that relative abundance is the same in control and dilute CEWAF treatments
b.dCEWAF: The log2 fold change in relative abundance between dilute CEWAF and control (> 0 means higher relative abundance in WAF)
sig.dCEWAF: A flag of 0 or 1 to indicate if there is a change due to the treatment (1= change); used to produce the summary table
qval.CEWAF: The multiple-comparisons corrected p-value for the hypothesis that relative abundance is the same in control and CEWAF treatments
b.CEWAF: The log2 fold change in relative abundance between CEWAF and control (> 0 means higher relative abundance in WAF)
sig.CEWAF: A flag of 0 or 1 to indicate if there is a change due to the treatment (1= change); used to produce the summary table
control: The estimated relative abundance (as %) of this taxon from kallisto in the control
WAF: The estimated relative abundance of this taxon from kallisto in the WAF treatment
DCEWAF: The estimated relative abundance of this taxon from kallisto in the dilute CEWAF treatment
CEWAF: The estimated relative abundance of this taxon from kallisto in the CEWAF treatment
Summary_GOMCOAST-taxonomic-composition-data-analysis.csv
Number with significant differential abundance relative to control: Category, n, WAF, dCEWAF, CEWAF
Proportion of total abundance (%): Control, WAF, DCEWAF, CEWAF
Kingdom-Phylum-Class: The taxonomic affiliation derived from column 1 of the raw data table
Count of Genus: The number of taxa (rows) aggregated from the raw data table to produce this summary
Sum of sig.WAF: The number of these taxa with significant differential abundance in WAF relative to control
Sum of sig.dCEWAF: The number of these taxa with significant differential abundance in dilute CEWAF relative to control
Sum of sig.CEWAF: The number of these taxa with significant differential abundance in CEWAF relative to control
Sum of control: The proportion (as a %) of total abundance attributed to this taxon in the control
Sum of WAF: The proportion (as a %) of total abundance attributed to this taxon in the WAF treatment
Sum of DCEWAF: The proportion (as a %) of total abundance attributed to this taxon in the dilute CEWAF treatment
Sum of CEWAF: The proportion (as a %) of total abundance attributed to this taxon in the CEWAF treatment|Twelve 100L mesocosm tanks were filled with Gulf of Mexico seawater collected from the Texas coastline, near TABS buoy R (29° 38.1000'N, 93° 38.5020'W) which is located ~100 miles away from Galveston (TX). Four treatments were prepared in triplicate. Control tanks were filled with seawater. Water accommodated fraction (WAF) of oil was prepared by mixing 25 mL (5 ml ~ every 30 min for 2.5 hrs) of Macondo surrogate oil into 130 L of seawater. Mixing ended 24 hrs. after the initial oil addition (Knap et al. 1986; Wade et al. 2017in preparation). The WAF was then introduced into the WAF mesocosm tanks and filled to 87 L and mixed. From these WAF tanks 6 L was removed for other experiments and analyses (2 L dark/light, 4 L hydrocarbon analyses). In order to make chemically enhanced water accommodated fraction (CEWAF), Corexit was mixed with oil in a ratio of 1:20 and 25 mL of this mixture (5 ml every 30 min for 2.5 hrs) of surrogate oil plus Corexit was added to 130 L of seawater. Mixing ended 24 hrs after the initial oil addition. The CEWAF was then introduced into the CEWAF mesocosm tanks and filled to 96 L and mixed. From these CEWAF tanks 13 L was removed for other experiments and analyses (7 L for the DCEWAF mesocosms, 2 L dark/light, 4 L hydrocarbon analyses). Diluted CEWAF (DCEWAF) was prepared by mixing 9 L of CEWAF with 78 L of the original seawater for a total volume of 87 L. From these DCEWAF tanks 6 L was removed for other experiments and analyses (2 L dark/light, 4 L hydrocarbon analyses). To the water in the 12 mesocosms, nutrients were added (final concentration f/20) and the tanks stirred. Banks of lights were placed behind each of the glass mesocosm tanks and a 12:12 light/dark cycle employed. Sampling commenced and defined as time zero. The estimated oil equivalents (EOE) were determined using Macondo surrogate oil as the calibration standard (Wade et al. 2011) for the fluorescence analyses (Horiba Scientific Aqualog Fluorometer). The EOE mean concentration of the three mesocosms for the control, WAF, DCEWAF and CEWAF at the start of the experiments were 0 mg/L, 0.26 mg/L, 2.74 mg/L and 41.5 mg/L, respectively. The EOE mean concentration of the three mesocosms for the in the control, WAF, DCEWAF and CEWAF after 72 hours were 0 mg/L , 0.06 mg/L, 1.03. and 17.3 mg/L, respectively.
The estimate of the relative abundance of active microbial eukaryotes within and across treatments is based on 18S RNA sequences from RNAseq data and reflects the relative presence and activity and detectability of each species. RNA was harvested from each of the 12 mesocosm tanks (3 replicate tanks for each treatment: control, WAF, CEWAF, DECWAF) 72 hours after the initiation of the experiment. Several hundred mL (250 to 4000 mL) were rapidly and gently filtered onto two 47mm, 8 m polycarbonate filters. We limited the total filtration time to 20 minutes. It took more time to filter water from the CEWAF tanks, and therefore less volume was filtered from this treatment. The filters and denaturation solution (Ambion Simply RNA) was added to Y-matrix bead beater tubes (MoBio). The samples were lysed using a SuperFastprep2 bead beater (30 seconds at the maximum setting) and immediately stored in a -80°C freezer. RNA was extracted by exposing samples immediately after thawing to 2 additional 30 second rounds in the SuperFastprep2 bead beater. The Ambion Total RNA kit (ThermoFisher AM1910) was used to extract RNA followed by DNA removal with the Ambion Turbo DNAfree kit (ThermoFisher AM1907) as per manufacturer instructions.
RNA was sequenced as 125 base pair paired-end reads using Illumina HiSeq 250 RNAseq by Genome Quebec. PolyA selection was used to remove the majority of the rRNA using the NEBNext Poly(A) mRNA magnetic isolation module kit from New England Biolabs. Approximately 2-10% of the original rRNA sequences pass through this step are sequenced (Abernathy and Overturf 2016). Trimmomatic was used to remove Illumina adapters and low quality bases were identified using Phred scores (Bolger, Lohse et al. 2014). Kraken and the Silva SSU rRNA Ref NR 99 database (release 123) were used to filter reads matching 18S sequences (Quast, Pruesse et al. 2013, Wood and Salzberg 2014). Kallisto and Sleuth were used to match, count and perform differential expression and relative abundance analysis for reads against the Silva 18S database (Bray, Pimentel et al. 2016, Pimentel, Bray et al. 2017). Three replicate mesocosms were sequenced for the control and WAF treatment, but following quality control only two replicates were available for the DCEWAF treatment and one replicate for the CEWAF treatment.
Each 18S sequence in the Silva database is identified with an accession code and a hierarchical taxonomic identification. All non-Eukaryota, Metazoa and Embryophyta sequences were removed from the analyses. The taxonomic identification in Silva is hierarchical but the identifications do not all have the same number of levels in the hierarchy so the phylum, order, or class, for example, are not readily extracted. Where possible we use the taxonomic hierarchy used by WoRMS in 2016/17. The genus and species name for each taxon in the Silva database (the last entry in the hierarchy) was used to search the World Registry of Marine Species (WoRMS, marinespecies.org). A variety of secondary sources were used to identify the taxonomic hierarchy for taxa without a match in WoRMS including the Global Names Index (GNI), the Pan-European Species Infrastructure (PESI), the Paleobiology database (Paleo), and the taxonomic hierarchy used by Wikipedia/Wikispecies. An automatic search for all taxa was performed using lifewatch.be on 2016 December 31 using WoRMS, GNI, PESI, and Paleo.||One sampling point was taken and analysed as described in the methods above.|There is no error quantified for the individual point estimates of relative abundance across treatments. The average relative abundance was estimated from replicate samples when available as described in the methods.|The original raw sequence data from the Illumina sequencer have been deposited at NCBI’s Short read archive (SRA) under BioProject PRJNA338185. The data are archived as BioSample SAMN08118040 and are accessible at http://www.ncbi.nlm.nih.gov/biosample/8118040
Jason Abernathy Trimmomatic: a flexible trimmer for Illumina sequence data
⋮
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