Oil Composition and nutrients for the GOMOO mesocosm using Gulf Of Mexico Open Ocean water

Aggregation and Degradation of Dispersants and Oil by Microbial Exopolymers (ADDOMEx)

DOI:
10.7266/N78P5XZD
 
UDI:
R4.x263.000:0038
Last Update:
Nov 01 2017 17:47 UTC
 
Dataset Author(s):
Terry L. Wade, Anthony H. Knap, Dawei Shi, Gerardo Gold-Bouchot, Maya E. Morales-McDevitt, Stephen T. Sweet, Peter H. Santschi and Antonietta Quigg
Point of Contact:
Wade, Terry
Texas A&M University / Geochemical and Environmental Research Group
833 Graham Road
College Station, Texas  77845
USA
terry@gerg.tamu.edu
Funding Source:
RFP-IV
 
Extent Description:
Dataset contains laboratory measurements of oil composition and concentration, seawater was collected from the Flower Gardens National Marine Sanctuary Area (27° 53.4180'N; 94° 2.2020'W).

Identified Submitted Review Available
3 3 3 3

Suggested Citation:

Terry L. Wade, Anthony H. Knap, Dawei Shi, Gerardo Gold-Bouchot, Maya E. Morales-McDevitt, Stephen T. Sweet, Peter H. Santschi and Antonietta Quigg (2017) Oil Composition and nutrients for the GOMOO mesocosm using Gulf Of Mexico Open Ocean water. Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University-Corpus Christi. doi:10.7266/N78P5XZD

Abstract:

Mesocosm experiments were performed with Gulf of Mexico seawater collected from the Flower Gardens National Marine Sanctuary Area (27° 53.4180'N; 94° 2.2020'W) which was amended with nutrients. Four treatments in triplicate were analyzed for oil composition: 1) Control, 2) Water accommodated oil fraction (WAF), 3) Chemically enhanced (Corexit 9500) water accommodated fraction (CEWAF), and 4) 10-fold diluted CEWAF (DCWAF). The oil composition data presented here are EOE (estimated oil equivalence using fluorescence), PAH (polycyclic aromatic hydrocarbons), and Total PAH. Additionally, nutrients for these treatments are also included.

Purpose:

To determine the concentration of oil constituents and dispersant and their degradation with time in four different mesocosm treatments from coastal waters. Also, to track the change in nutrients during the same time course.

Theme Keywords:

oil composition, oil concentration, PAH, TPAH, n-alkanes, nutrients, polycyclic aromatic hydrocarbons, mesocosm, EOE, estimated oil equivalence

File Format:

csv

Filename:

M3.7z (16.4 KB)

Dataset Downloads:

3

Oil Composition and nutrients for the GOMOO mesocosm using Gulf Of Mexico Open Ocean water



Identification Information
Distribution Information
Metadata Maintenance Information

Metadata: 
  File identifier: 
      R4.x263.000-0038-metadata.xml
  Language: 
      eng; USA
  Character set: 
    Character set code: 
      utf8
  Hierarchy level: 
    Scope code: 
      dataset
  Metadata author: 
    Responsible party: 
      Individual name: 
          Kathleen (Kathy) Schwehr
      Organisation name: 
          Texas A&M University at Galveston / Marine Sciences Department
      Position name: 
          Associate Research Scientist
      Contact info: 
        Contact: 
          Phone: 
            Telephone: 
              Voice: 
                  4097404452
              Facsimile: 
          Address: 
            Address: 
              Delivery point: 
                  P.O. Box 1675
              City: 
                  Galveston
              Administrative area: 
                  Texas
              Postal code: 
                  77553
              Country: 
                  USA
              Electronic mail address: 
                  schwehrk@tamug.edu
      Role: 
        Role code: 
          pointOfContact
  Date stamp: 
      2018-12-07T21:44:24+00:00
  Metadata standard name: 
      ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
  Metadata standard version: 
      ISO 19115-2:2009(E)
  Dataset URI: 
      https://data.gulfresearchinitiative.org/metadata/R4.x263.000:0038
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Identification info: Data identification: Citation: Citation: Title: Oil Composition and nutrients for the GOMOO mesocosm using Gulf Of Mexico Open Ocean water Alternate title: Oil Composition and nutrients for the GOMOO mesocosm using Open Ocean water Date: Date: Date: 2017-10-25 Date type: Date type code: creation Abstract: Mesocosm experiments were performed with Gulf of Mexico seawater collected from the Flower Gardens National Marine Sanctuary Area (27° 53.4180'N; 94° 2.2020'W) which was amended with nutrients. Four treatments in triplicate were analyzed for oil composition: 1) Control, 2) Water accommodated oil fraction (WAF), 3) Chemically enhanced (Corexit 9500) water accommodated fraction (CEWAF), and 4) 10-fold diluted CEWAF (DCWAF). The oil composition data presented here are EOE (estimated oil equivalence using fluorescence), PAH (polycyclic aromatic hydrocarbons), and Total PAH. Additionally, nutrients for these treatments are also included. Purpose: To determine the concentration of oil constituents and dispersant and their degradation with time in four different mesocosm treatments from coastal waters. Also, to track the change in nutrients during the same time course. Status: Progress code: completed Point of contact: Responsible party: Individual name: Terry Wade Organisation name: Texas A&M University / Geochemical and Environmental Research Group Position name: Deputy Director Contact info: Contact: Phone: Telephone: Voice: 9795712279 Facsimile: Address: Address: Delivery point: 833 Graham Road City: College Station Administrative area: Texas Postal code: 77845 Country: USA Electronic mail address: terry@gerg.tamu.edu Role: Role code: pointOfContact Descriptive keywords: Keywords: Keyword: oil composition Keyword: oil concentration Keyword: PAH Keyword: TPAH Keyword: n-alkanes Keyword: nutrients Keyword: polycyclic aromatic hydrocarbons Keyword: mesocosm Keyword: EOE Keyword: estimated oil equivalence Type: Keyword type code: theme Descriptive keywords: Keywords: Keyword: inapplicable Type: Keyword type code: place Language: eng; USA Topic category: Topic category code: oceans Topic category: Topic category code: biota Topic category: Topic category code: environment Extent: Extent: Description: Dataset contains laboratory measurements of oil composition and concentration, seawater was collected from the Flower Gardens National Marine Sanctuary Area (27° 53.4180'N; 94° 2.2020'W). Supplemental Information: EOE (Estimated Oil Equivalence, mg/L); PAH (Polycyclic Aromatic Hydrocarbons, ng/L); Total PAH (ng/L); WAF (Water Accommodated Fraction); CEWAF (Chemically Enhanced Water Accommodated Fraction); DCEWAF (Diluted Chemically Enhanced Water Accommodated Fraction); nutrient data are given in 2 conc. or concentration formats: micromoles/L (umol/L) and mg/L with respect to the listed standard. NO3- is nitrate, N is nitrogen, HPO4= is hydrogen phosphate, P is Phosphorus, HSIO3- is hydrogen silicate, SiO3 refers to silicate, NH4+ is ammonium, NO2- is nitrite, Urea, Nitrate+Nitrite, Time (hr) intervals were at 0, 24, 48, 72, 96; Volume (L); LB=light bottle; DB=dark bottle; -999 = below detection limit, Individual alkanes (ng/L), Total alkanes (ng/L), Volume (L), Extraction date (MM/DD/YYYY), Analysis date (MM/DD/YYYY HH:MM:SS), Total resolved (ug/L), Total TPH (ug/L), Total UCM (ug/L) Treatments: O = WAF DM = diluted CWAF M = CWAF |Twelve 100L mesocosm tanks were filled with Gulf of Mexico seawater collected from the Flower Gardens National Marine Sanctuary Area (27° 53.4180'N; 94° 2.2020'W) which is located ~120 miles off the coast of Galveston (TX). Four treatments were prepared in triplicate. Control tanks were filled with seawater. Water accommodated fraction (WAF) of oil was prepared by mixing 25 mL (5 ml ~ every 30 min for 2.5 hrs) of Macondo surrogate oil into 130 L of seawater. Mixing ended 24 hrs after the initial oil addition (Knap et al. 1986; Wade et al. 2017 in preparation). The WAF was then introduced into the WAF mesocosm tanks and filled to 87 L and mixed. From these WAF tanks 6 L was removed for other experiments and analyses (2 L light /dark bottles, 4 L hydrocarbon analyses). In order to make chemically enhanced water accommodated fraction (CEWAF), Corexit was mixed with oil in a ratio of 1:20 and 25 mL of this mixture (5 ml every 30 min for 2.5 hrs) of surrogate oil plus Corexit was added to 130 L of seawater. Mixing ended 24 hrs after the initial oil addition. The CEWAF was then introduced into the CEWAF mesocosm tanks and filled to 96 L and mixed. From these CEWAF tanks 13 L was removed for other experiments and analyses (7 L for the DCEWAF mesocosms, 2 L light/dark, 4 L hydrocarbon analyses). Diluted CEWAF (DCEWAF) was prepared by mixing 9 L of CEWAF with 78 L of the original seawater for a total volume of 87 L. From these DCEWAF tanks 6 L was removed for other experiments and analyses (2 L light/dark, 4 L hydrocarbon analyses). To the water in the 12 mesocosms, nutrients were added (final concentration f/20) and the tanks stirred. Banks of lights were placed behind each of the glass mesocosm tanks and a 12:12 light/dark cycle employed. Sampling commenced and defined as time zero. The estimated oil equivalents (EOE) were determined using Macondo surrogate oil as the calibration standard (Wade et al. 2011) for the fluorescence analyses (Horiba Scientific Aqualog Fluorometer). The EOE mean concentration of the three mesocosms for the control, WAF, DCEWAF and CEWAF at the start of the experiments were 0 mg/L, 0.26 mg/L, 2.74 mg/L and 41.5 mg/L, respectively. The EOE mean concentration of the three mesocosms for the in the control, WAF, DCEWAF and CEWAF after 72 hours were 0 mg/L , 0.06 mg/L, 1.03. and 17.3 mg/L, respectively. Estimated Oil Equivalence (EOE) The estimated oil equivalents (EOE) were determined by fluorescence (Wade et al. 2011) using Macondo surrogate oil as a standard to produce calibration curves at 5 to 7 concentrations. Water samples (5 to 20 ml) were extracted with 5 ml of dichloromethane. An aliquot of the extract was placed in a cuvette for fluorescence analyses (Horiba Scientific Aqualog Fluorometer). The EOE were determined from the calibration curve (Wade et al. 2011). Samples with florescence responses that exceeded the calibration curve were diluted so that their florescence was within the calibration range. Samples were taken at the beginning and end of the experiment and at intervals in between and at the same time point as measurements of other parameters during the experiment. PAH (polycyclic aromatic hydrocarbons) and Total PAH extraction of the sample with dichloromethane. The samples are then purified using silica/alumina columns. analyzed. The analysis is performed with gas chromatography to separate the individual aromatic compounds and with a mass selective detector in the selected ion mode for identification and quantification. The parent PAH (e.g. naphthalene) and its alkylated homolog’s (e.g. C-1, C-2, C-3 and C-4) are also determined. These analyses can be used to distinguish between petroleum and combustion sources or a mixture of these sources. The individual PAH compounds and their alkylated homologs are reported.|EOE fluorescence analyses was carried out using a Horiba Scientific Aqualog Fluorometer. Total PAH were determined by gas chromatography (GC) with a flame ionization detector (GC/FID) and PAHs by GC with a mass selective detector [Short et al., 1996].|Time is in number of hours as indicated.||Short, J. W., T. J. Jackson, M. L. Larsen, and T. L. Wade (1996), Analytical methods used for the analysis of hydrocarbons in crude oil tissues, sediments, and seawater collected for the natural resources damage assessment of the Exxon Valdez oil spill, in Proceedings of the Exxon Valdez Oil Spill Symposium, edited by S. D. Rice et al., pp. 140–148, Am. Fish. Soc., Bethesda, Md. Wade, T. L., S. T. Sweet, J. N. Walpert, J. L. Sericano, J. J. Singer, and N. L. Guinasso Jr. (2011), Evaluation of possible inputs of oil from the Deepwater Horizon spill to the Loop Current and associated eddies in the Gulf of Mexico, in Monitoring and Modeling the Deepwater Horizon Oil Spill: A Record-Breaking Enterprise, Geophys. Monogr. Ser., doi:10.1029/2011GM 001095. Knap, A. H., T. D. Sleeter, R. E. Dodge, S. C. Wyers, H. R. Frith, and S. R. Smith. 1983. The effects of oil spills and dispersants use on corals: A review and multidisciplinary experimental approach. Oil and Petrochemical Pollution 1: 157–169.
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Distribution info: Distribution: Distributor: Distributor: Distributor contact: Responsible party: Organisation name: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC) Contact info: Contact: Phone: Telephone: Voice: 3618253604 Address: Address: Delivery point: 6300 Ocean Drive City: Corpus Christi Administrative area: TX Postal code: 78412 Country: USA Electronic mail address: griidc@gomri.org Online Resource: Online Resource: Linkage: URL: https://data.gulfresearchinitiative.org Role: Role code: distributor Distributor format: Format: Name: csv Version: inapplicable File decompression technique: 7z Distributor transfer options: Digital transfer options: Transfer size: 0.0164 Online: Online Resource: Linkage: URL: https://data.gulfresearchinitiative.org/data/R4.x263.000:0038 Protocol: https Name: Data Landing Page Description: GRIIDC dataset landing page Function: Online function code: information
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Metadata maintenance: Maintenance information: Maintenance and update frequency: unknown Maintenance note: This ISO metadata record was automatically generated from information provided to GRIIDC for dataset: R4.x263.000:0038 on 2019-07-22T21:38:05-05:00
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