Particle counts in different size fractions taken during mesocosms GOMOO, GOM open ocean, and GOMCOAST, GOM coastal waters

Aggregation and Degradation of Dispersants and Oil by Microbial Exopolymers (ADDOMEx)

DOI:
10.7266/N7CF9NK6
 
UDI:
R4.x263.000:0025
Last Update:
Jan 24 2018 22:13 UTC
 
Dataset Author(s):
Laura Bretherton
Point of Contact:
Bretherton, Laura
Mount Allison University / Environmental Science
67 York St.
Sackville, New Brunswick  E4L 1E6
CAN
lmjbre@gmail.com
Funding Source:
RFP-IV
 
Extent Description:
Dataset contains laboratory measurements of particle counts from material originating from the field.

Identified Submitted In-Review Available
3 3 3 3

Suggested Citation:

Laura Bretherton. 2018. Particle counts in different size fractions taken during mesocosms GOMOO, GOM open ocean, and GOMCOAST, GOM coastal waters. Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7CF9NK6

Abstract:

Aggregation is a phenomenon that has been observed following oil spills. The size and number of aggregates can change with specific microbial communities, or the presence of chemical dispersants such as Corexit. In order to assess the impact of oil exposure on the formation of small aggregates (<100um), two mesocosm experiments were conducted during July 2016; GOMOO (Gulf of Mexico Open Ocean) and GOMCOAST (Gulf of Mexico Coastal waters). Oil and dispersant exposure were facilitated using the water accommodated fraction (WAF) of oil, a chemically enhanced WAF (CEWAF, mixture of oil and Corexit) and a dilute CEWAF (DCEWAF). 15mL aliquots were taken every 24h from each mesocosm for immediate analysis on a Z2 dual-threshold Coulter counter (Beckman Coulter). The Coulter counter was outfitted with a 100µm aperture, and particles were counted in three ranges; 10-20µm, 20-50µm and >50µm. The data presented will be particle counts (particles/mL) over time within each size fraction. Estimated oil equivalents are reported in GRIIDC datasets R4.x263.000:0038 and R4.x263.000:0039.

Purpose:

To characterize the dynamics of small sized particles in order to understand whether or not aggregation processes are occurring.

Theme Keywords:

aggregation, particles, oil spill

File Format:

csv

Filename:

Total particle counts GOMCOAST GOMOO.csv (10.53 KB)

Dataset Downloads:

3

Particle counts in different size fractions taken during mesocosms GOMOO, GOM open ocean, and GOMCOAST, GOM coastal waters



Identification Information
Distribution Information
Metadata Maintenance Information

Metadata: 
  File identifier: 
      R4.x263.000-0025-metadata.xml
  Language: 
      eng; USA
  Character set: 
    Character set code: 
      utf8
  Hierarchy level: 
    Scope code: 
      dataset
  Metadata author: 
    Responsible party: 
      Individual name: 
          Laura Bretherton
      Organisation name: 
          Mount Allison University / Environmental Science
      Position name: 
          Postdoctoral Researcher
      Contact info: 
        Contact: 
          Phone: 
            Telephone: 
              Voice: 
              Facsimile: 
          Address: 
            Address: 
              Delivery point: 
                  67 York St.
              City: 
                  Sackville
              Administrative area: 
                  New Brunswick
              Postal code: 
                  E4L 1E6
              Country: 
                  CAN
              Electronic mail address: 
                  lmjbre@gmail.com
      Role: 
        Role code: 
          pointOfContact
  Date stamp: 
      2018-08-11T13:44:32+00:00
  Metadata standard name: 
      ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
  Metadata standard version: 
      ISO 19115-2:2009(E)
  Dataset URI: 
      https://data.gulfresearchinitiative.org/metadata/R4.x263.000:0025
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Identification info: Data identification: Citation: Citation: Title: Particle counts in different size fractions taken during mesocosms GOMOO, GOM open ocean, and GOMCOAST, GOM coastal waters Alternate title: Date: Date: Date: 2018-03-23 Date type: Date type code: publication Identifier: Identifier: Code: Anchor: xlink: https://dx.doi.org/10.7266/N7CF9NK6 title: DOI doi:10.7266/N7CF9NK6 Abstract: Aggregation is a phenomenon that has been observed following oil spills. The size and number of aggregates can change with specific microbial communities, or the presence of chemical dispersants such as Corexit. In order to assess the impact of oil exposure on the formation of small aggregates (<100um), two mesocosm experiments were conducted during July 2016; GOMOO (Gulf of Mexico Open Ocean) and GOMCOAST (Gulf of Mexico Coastal waters). Oil and dispersant exposure were facilitated using the water accommodated fraction (WAF) of oil, a chemically enhanced WAF (CEWAF, mixture of oil and Corexit) and a dilute CEWAF (DCEWAF). 15mL aliquots were taken every 24h from each mesocosm for immediate analysis on a Z2 dual-threshold Coulter counter (Beckman Coulter). The Coulter counter was outfitted with a 100µm aperture, and particles were counted in three ranges; 10-20µm, 20-50µm and >50µm. The data presented will be particle counts (particles/mL) over time within each size fraction. Estimated oil equivalents are reported in GRIIDC datasets R4.x263.000:0038 and R4.x263.000:0039. Purpose: To characterize the dynamics of small sized particles in order to understand whether or not aggregation processes are occurring. Status: Progress code: completed Point of contact: Responsible party: Individual name: Laura Bretherton Organisation name: Mount Allison University / Environmental Science Position name: Postdoctoral Researcher Contact info: Contact: Phone: Telephone: Voice: Facsimile: Address: Address: Delivery point: 67 York St. City: Sackville Administrative area: New Brunswick Postal code: E4L 1E6 Country: CAN Electronic mail address: lmjbre@gmail.com Role: Role code: pointOfContact Point of contact: Responsible party: Individual name: Antonietta Quigg Organisation name: Texas A&M University at Galveston / Marine Biology Department Position name: Professor Contact info: Contact: Phone: Telephone: Voice: 4097404990 Facsimile: Address: Address: Delivery point: P.O. Box 1675 City: Galveston Administrative area: Texas Postal code: 77553 Country: USA Electronic mail address: quigga@tamug.edu Role: Role code: pointOfContact Descriptive keywords: Keywords: Keyword: aggregation Keyword: particles Keyword: oil spill Type: Keyword type code: theme Descriptive keywords: Keywords: Keyword: Gulf of Mexico Keyword: Galveston Keyword: Texas Type: Keyword type code: place Resource constraints: title: Cite As Constraints: Use limitation: Laura Bretherton. 2018. Particle counts in different size fractions taken during mesocosms GOMOO, GOM open ocean, and GOMCOAST, GOM coastal waters. Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7CF9NK6 Resource constraints: title: CC0 License Legal constraints: Use constraints: Restriction code: licenceUnrestricted Other constraints: This information is released under the Creative Commons license - No Rights Reserved - CC0 1.0 Universal (https://creativecommons.org/publicdomain/zero/1.0/). The person who associated a work with this deed has dedicated the work to the public domain by waiving all of his or her rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law. You can copy, modify, distribute and perform the work, even for commercial purposes, all without asking permission. Resource constraints: title: Liability and Warranty Legal constraints: Other constraints: All materials on this website are made available to GRIIDC and in turn to you "as-is." Content may only be submitted by an individual who represents and warrants that s/he has sufficient rights to be able to make the content available under a CC0 waiver. There is no warranty (expressed or implied) to these materials, their title, accuracy, non-infringement of third party rights, or fitness for any particular purpose, including the performance or results you may obtain from their use. Use these materials at your own risk. Under no circumstances shall GRIIDC be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use or the inability to use either this website or the materials available via this website. If you are dissatisfied with any website feature, content, or terms of use, your sole and exclusive remedy is to discontinue use. Aggregation Info: AggregateInformation: Aggregate Data Set Name: title: Related Publication Citation Citation: Title: Bretherton, L., Kamalanathan, M., Genzer, J., Hillhouse, J., Setta, S., Liang, Y., … Quigg, A. (2019). Response of natural phytoplankton communities exposed to crude oil and chemical dispersants during a mesocosm experiment. Aquatic Toxicology, 206, 43–53. doi:10.1016/j.aquatox.2018.11.004 Date: inapplicable Aggregate Data Set Identifier: title: Related Publication DOI Identifier: Code: Anchor: xlink: https://dx.doi.org/10.1016/j.aquatox.2018.11.004 title: DOI doi:10.1016/j.aquatox.2018.11.004 Association Type: Association type code: crossReference Language: eng; USA Topic category: Topic category code: oceans Topic category: Topic category code: environment Extent: Extent: Description: Dataset contains laboratory measurements of particle counts from material originating from the field. Supplemental Information: Mesocosm, GOMCOAST (Gulf of Mexico COASTal water) or GOMOO (Gulf of Mexico Open Ocean water); Time (hours); Treatment, C (Control), O (WAF), M (CEWAF), DM (DCEWAF); Size Fraction (µm); Particle Count (particles/mL)|GOMOO Mesocosm methods: Twelve 100L mesocosm tanks were filled with Gulf of Mexico seawater collected from the Flower Gardens National Marine Sanctuary Area (27° 53.4180'N; 94° 2.2020'W) which is located ~120 miles off the coast of Galveston (TX). Four treatments were prepared in triplicate. Control tanks were filled with seawater. Water accommodated fraction (WAF) of oil was prepared by mixing 25 mL (5 ml ~ every 30 min for 2.5 hrs) of Macondo surrogate oil into 130 L of seawater. Mixing ended 24 hrs after the initial oil addition (Knap et al. 1986; Wade et al. 2017 in preparation). The WAF was then introduced into the WAF mesocosm tanks and filled to 87 L and mixed. From these WAF tanks 6 L was removed for other experiments and analyses (2 L light /dark bottles, 4 L hydrocarbon analyses). In order to make chemically enhanced water accommodated fraction (CEWAF), Corexit was mixed with oil in a ratio of 1:20 and 25 mL of this mixture (5 ml every 30 min for 2.5 hrs) of surrogate oil plus Corexit was added to 130 L of seawater. Mixing ended 24 hrs after the initial oil addition. The CEWAF was then introduced into the CEWAF mesocosm tanks and filled to 96 L and mixed. From these CEWAF tanks 13 L was removed for other experiments and analyses (7 L for the DCEWAF mesocosms, 2 L light/dark, 4 L hydrocarbon analyses). Diluted CEWAF (DCEWAF) was prepared by mixing 9 L of CEWAF with 78 L of the original seawater for a total volume of 87 L. From these DCEWAF tanks 6 L was removed for other experiments and analyses (2 L light/dark, 4 L hydrocarbon analyses). To the water in the 12 mesocosms, nutrients were added (final concentration f/20) and the tanks stirred. Banks of lights were placed behind each of the glass mesocosm tanks and a 12:12 light/dark cycle employed. Sampling commenced and defined as time zero. The estimated oil equivalents (EOE) were determined using Macondo surrogate oil as the calibration standard (Wade et al. 2011) for the fluorescence analyses (Horiba Scientific Aqualog Fluorometer). The EOE mean concentration of the three mesocosms for the control, WAF, DCEWAF and CEWAF at the start of the experiments were 0 mg/L, 0.26 mg/L, 2.74 mg/L and 41.5 mg/L, respectively. The EOE mean concentration of the three mesocosms for the in the control, WAF, DCEWAF and CEWAF after 72 hours were 0 mg/L , 0.06 mg/L, 1.03. and 17.3 mg/L, respectively. GOMCOAST Mesocosm methods: Twelve 100L mesocosm tanks were filled with Gulf of Mexico seawater collected from the Texas coastline, near TABS buoy R (29° 38.1000'N, 93° 38.5020'W) which is located ~100 miles away from Galveston (TX). Four treatments were prepared in triplicate. Control tanks were filled with seawater. Water accommodated fraction (WAF) of oil was prepared by mixing 25 mL (5 ml ~ every 30 min for 2.5 hrs) of Macondo surrogate oil into 130 L of seawater. Mixing ended 24 hrs. after the initial oil addition (Knap et al. 1986; Wade et al. 2017in preparation). The WAF was then introduced into the WAF mesocosm tanks and filled to 87 L and mixed. From these WAF tanks 6 L was removed for other experiments and analyses (2 L dark/light, 4 L hydrocarbon analyses). In order to make chemically enhanced water accommodated fraction (CEWAF), Corexit was mixed with oil in a ratio of 1:20 and 25 mL of this mixture (5 ml every 30 min for 2.5 hrs) of surrogate oil plus Corexit was added to 130 L of seawater. Mixing ended 24 hrs after the initial oil addition. The CEWAF was then introduced into the CEWAF mesocosm tanks and filled to 96 L and mixed. From these CEWAF tanks 13 L was removed for other experiments and analyses (7 L for the DCEWAF mesocosms, 2 L dark/light, 4 L hydrocarbon analyses). Diluted CEWAF (DCEWAF) was prepared by mixing 9 L of CEWAF with 78 L of the original seawater for a total volume of 87 L. From these DCEWAF tanks 6 L was removed for other experiments and analyses (2 L dark/light, 4 L hydrocarbon analyses). To the water in the 12 mesocosms, nutrients were added (final concentration f/20) and the tanks stirred. Banks of lights were placed behind each of the glass mesocosm tanks and a 12:12 light/dark cycle employed. Sampling commenced and defined as time zero. The estimated oil equivalents (EOE) were determined using Macondo surrogate oil as the calibration standard (Wade et al. 2011) for the fluorescence analyses (Horiba Scientific Aqualog Fluorometer). The EOE mean concentration of the three mesocosms for the control, WAF, DCEWAF and CEWAF at the start of the experiments were 0 mg/L, 0.26 mg/L, 2.74 mg/L and 41.5 mg/L, respectively. The EOE mean concentration of the three mesocosms for the in the control, WAF, DCEWAF and CEWAF after 72 hours were 0 mg/L , 0.06 mg/L, 1.03. and 17.3 mg/L, respectively. A 20 mL sample was taken from each mesocosm tank every 24 h, and immediately analyzed using a Coulter counter (Beckman-Coulter, Z2) fitted with a 100 µm aperture. Particle concentrations were measured in three size fractions; 5-10 µm, 10-20 µm and 20-50 µm. The particle coincidence at the aperture was monitored to ensure it remained below 5%. Particle coincidence is an instance of more than one particle entering the aperture simultaneously, which can underestimate particle counts. Samples where particle concentrations were high enough to cause particle coincidence of >5% were diluted with filtered seawater (0.2 µm, nylon), and only ever happened in CEWAF tanks. Wade, T. L., S. T. Sweet, J. N. Walpert, J. L. Sericano, J. J. Singer, and N. L. Guinasso Jr. (2011), Evaluation of possible inputs of oil from the Deepwater Horizon spill to the Loop Current and associated eddies in the Gulf of Mexico, in Monitoring and Modeling the Deepwater Horizon Oil Spill: A Record-Breaking Enterprise, Geophys. Monogr. Ser., doi:10.1029/2011GM 001095. Knap, A. H., Burns, K.A., Dawson, R., Ehrhardt, M., and Palmork, K.H. (1986), Dissolved/dispersed Hydrocarbons, Tarballs and the Surface Microlayer: Experiences from an IOC/UNEP Workshop in Bermuda. Mar. Pol. Bull., 17(7): 313-319.|Z2 dual threshold coulter counter (Beckman-Coulter)|A 20 mL sample was taken from each mesocosm tank every 24 h||
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Distribution info: Distribution: Distributor: Distributor: Distributor contact: Responsible party: Organisation name: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC) Contact info: Contact: Phone: Telephone: Voice: 3618253604 Address: Address: Delivery point: 6300 Ocean Drive City: Corpus Christi Administrative area: TX Postal code: 78412 Country: USA Electronic mail address: griidc@gomri.org Online Resource: Online Resource: Linkage: URL: https://data.gulfresearchinitiative.org Role: Role code: distributor Distributor format: Format: Name: csv Version: inapplicable File decompression technique: Distributor transfer options: Digital transfer options: Transfer size: 0.0105 Online: Online Resource: Linkage: URL: https://data.gulfresearchinitiative.org/data/R4.x263.000:0025 Protocol: https Name: Data Landing Page Description: GRIIDC dataset landing page Function: Online function code: information
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Metadata maintenance: Maintenance information: Maintenance and update frequency: unknown Maintenance note: This ISO metadata record was automatically generated from information provided to GRIIDC for dataset: R4.x263.000:0025 on 2020-09-24T10:58:59-05:00
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