Oil Composition and nutrients for the COAST mesocosm study, COASTal water with added coastal microbial concentrate

Aggregation and Degradation of Dispersants and Oil by Microbial Exopolymers (ADDOMEx)

DOI:
10.7266/N7PK0D64
 
UDI:
R4.x263.000:0019
Last Update:
Apr 28 2017 19:53 UTC
 
Dataset Author(s):
Terry L. Wade, Anthony H. Knap, Dawei Shi, Gerardo Gold-Bouchot, Maya E. Morales-McDevitt, Stephen T. Sweet, Peter H. Santschi and Antonietta Quigg
Point of Contact:
Wade, Terry
Texas A&M University / Geochemical and Environmental Research Group
833 Graham Road
College Station, Texas  77845
USA
terry@gerg.tamu.edu
Funding Source:
RFP-IV
 
Extent Description:
Dataset contains laboratory measurements of oil composition and concentration, seawater was collected 10/17/15 from 8 kilometers off shore south of Galveston.

Identified Submitted Review Available
3 3 3 3

Suggested Citation:

Terry L. Wade, Anthony H. Knap, Dawei Shi, Gerardo Gold-Bouchot, Maya E. Morales-McDevitt, Stephen T. Sweet, Peter H. Santschi and Antonietta Quigg (2017) Oil Composition and nutrients for the COAST mesocosm study, COASTal water with added coastal microbial concentrate. Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University-Corpus Christi. doi:10.7266/N7PK0D64

Abstract:

Mesocosm experiments were performed with seawater collected from the NGOM and enriched with coastal plankton collected from Galveston Bay. The four treatments in triplicate were analyzed for oil composition were 1) Control, 2) Water accommodated oil fraction (WAF), 3) Chemically enhanced (Corexit 9500) water accommodated fraction (CEWAF), and 4) 10-fold diluted CEWAF (DCWAF). The oil composition data presented here are EOE (estimated oil equivalence using fluorescence), PAH (polycyclic aromatic hydrocarbons), and Total PAH. Additionally, nutrients for these treatments are also included. Solid-state 13C NMR spectroscopic analysis data are available in GRIIDC dataset UDI: R4.x263.000:0022.

Purpose:

To determine the concentration of oil constituents and dispersant and their degradation with time in four different mesocosm treatments from coastal waters. Also, to track the change in nutrients during the same time course.

Theme Keywords:

oil composition, oil concentration, EOE, PAH, polycyclic aromatic hydrocarbons, nutrients, mesocosm

File Format:

csv

Filename:

COAST EOE PAH TotalPAH NUTS.zip (5.17 KB)

Dataset Downloads:

10

Oil Composition and nutrients for the COAST mesocosm study, COASTal water with added coastal microbial concentrate



Identification Information
Distribution Information
Metadata Maintenance Information

Metadata: 
  File identifier: 
      R4.x263.000-0019-metadata.xml
  Language: 
      eng; USA
  Character set: 
    Character set code: 
      utf8
  Hierarchy level: 
    Scope code: 
      dataset
  Metadata author: 
    Responsible party: 
      Individual name: 
          Terry Wade
      Organisation name: 
          Texas A&M University / Geochemical and Environmental Research Group
      Position name: 
          Deputy Director
      Contact info: 
        Contact: 
          Phone: 
            Telephone: 
              Voice: 
                  9795712279
              Facsimile: 
          Address: 
            Address: 
              Delivery point: 
                  833 Graham Road
              City: 
                  College Station
              Administrative area: 
                  Texas
              Postal code: 
                  77845
              Country: 
                  USA
              Electronic mail address: 
                  terry@gerg.tamu.edu
      Role: 
        Role code: 
          pointOfContact
  Date stamp: 
      2018-08-11T13:35:50+00:00
  Metadata standard name: 
      ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
  Metadata standard version: 
      ISO 19115-2:2009(E)
  Dataset URI: 
      https://data.gulfresearchinitiative.org/metadata/R4.x263.000:0019
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Identification info: Data identification: Citation: Citation: Title: Oil Composition and nutrients for the COAST mesocosm study, COASTal water with added coastal microbial concentrate Alternate title: Date: Date: Date: 2017-01-27 Date type: Date type code: publication Abstract: Mesocosm experiments were performed with seawater collected from the NGOM and enriched with coastal plankton collected from Galveston Bay. The four treatments in triplicate were analyzed for oil composition were 1) Control, 2) Water accommodated oil fraction (WAF), 3) Chemically enhanced (Corexit 9500) water accommodated fraction (CEWAF), and 4) 10-fold diluted CEWAF (DCWAF). The oil composition data presented here are EOE (estimated oil equivalence using fluorescence), PAH (polycyclic aromatic hydrocarbons), and Total PAH. Additionally, nutrients for these treatments are also included. Solid-state 13C NMR spectroscopic analysis data are available in GRIIDC dataset UDI: R4.x263.000:0022. Purpose: To determine the concentration of oil constituents and dispersant and their degradation with time in four different mesocosm treatments from coastal waters. Also, to track the change in nutrients during the same time course. Status: Progress code: completed Point of contact: Responsible party: Individual name: Terry Wade Organisation name: Texas A&M University / Geochemical and Environmental Research Group Position name: Deputy Director Contact info: Contact: Phone: Telephone: Voice: 9795712279 Facsimile: Address: Address: Delivery point: 833 Graham Road City: College Station Administrative area: Texas Postal code: 77845 Country: USA Electronic mail address: terry@gerg.tamu.edu Role: Role code: pointOfContact Descriptive keywords: Keywords: Keyword: oil composition Keyword: oil concentration Keyword: EOE Keyword: PAH Keyword: polycyclic aromatic hydrocarbons Keyword: nutrients Keyword: mesocosm Type: Keyword type code: theme Descriptive keywords: Keywords: Keyword: inapplicable Type: Keyword type code: place Language: eng; USA Topic category: Topic category code: oceans Topic category: Topic category code: environment Extent: Extent: Description: Dataset contains laboratory measurements of oil composition and concentration, seawater was collected 10/17/15 from 8 kilometers off shore south of Galveston. Supplemental Information: EOE (Estimated Oil Equivalence, mg/L); PAH (Polycyclic Aromatic Hydrocarbons, ng/L); Total PAH (ng/L); WAF (Water Accommodated Fraction); CEWAF (Chemically Enhanced Water Accommodated Fraction); DCEWAF (Diluted Chemically Enhanced Water Accommodated Fraction); nutrient data are given in 2 conc. or concentration formats: micromoles/L (umol/L) and mg/L with respect to the listed standard. NO3- is nitrate, N is nitrogen, HPO4= is hydrogen phosphate, P is Phosphorus, HSIO3- is hydrogen silicate, SiO3 refers to silicate, NH4+ is ammonium, NO2- is nitrite, Time (hr), Volume (L) |The seawater was collected from the same location as that used in TeCOAST Mesocsom, but was collected on October 17 2015, from 8 kilometers off shore south of Galveston (TX) in the Gulf of Mexico. The salinity was 31. The seawater was processed through a charcoal filter to remove large particles and debris. Four treatments were prepared in triplicate. Control tanks were filled with seawater and a plankton mixture. Water accommodated fraction (WAF) of oil was prepared by mixing 25 mL (5 ml ~ every 30 min for 2.5 hrs) of Macondo surrogate oil into 130 L of seawater then mixing for 12 to 24 hrs (Knap et al. 1986; Knap et al. 2016 in preparation). The WAF was then introduced into the WAF mesocosm tanks and filled to 87 L and mixed. From these WAF tanks 6 L was removed for other experiments and analyses (2L for roller tables, 2 L dark/light, 4 L hydrocarbon analyses). In order to make chemically enhanced water accommodated fraction (CEWAF), Corexit was mixed with oil in a ratio of 1:20 and 25 mL of this mixture (5 ml every 30 min for 2.5 hrs) of surrogate oil plus Corexit was added to 130 L of seawater which was mixed for 8 to 24 hrs prior to being transferred to the mesocosm tanks. The CEWAF was then introduced into the CEWAF mesocosm tanks and filled to 96 L and mixed. From these CEWAF tanks 15 L was removed for other experiments and analyses (9 L for the DCEWAF mesocosms, 2L for roller tables, 2 L dark/light, 4 L hydrocarbon analyses). Diluted CEWAF (DCEWAF) was prepared by mixing 9 L of CEWAF with 78 L of the original seawater for a total volume of 87 L. From these DCEWAF tanks 6 L was removed for other experiments and analyses (2L for roller tables, 2 L dark/light, 4 L hydrocarbon analyses). Plankton (≥63 µm) were collected using a net and transferred into polycarbonate bottles. This concentrated plankton mass was introduced to the tanks and stirred (2 L to each final volume 83 L) immediately prior to starting the experiments. The EOE mean concentration of the three mesocosms for the control, WAF, DCEWAF and CEWAF at the start of the experiments were 0 mg/L, 0.26 mg/L, 2.74 mg/L and 41.5 mg/L, respectively. The EOE mean concentration of the three for the control, WAF, DCEWAF and CEWAF mesocosms after 72 hours were 0 mg/L, 0.06 mg/L, 1.03, and 17.3 mg/L, respectively. Estimated Oil Equivalence (EOE) The estimated oil equivalents (EOE) were determined by fluorescence (Wade et al. 2011) using Macondo surrogate oil as a standard to produce calibration curves at 5 to 7 concentrations. Water samples (5 to 20 ml) were extracted with 5 ml of dichloromethane. An aliquot of the extract was placed in a cuvette for fluorescence analyses (Horiba Scientific Aqualog Fluorometer). The EOE were determined from the calibration curve (Wade et al. 2011). Samples with florescence responses that exceeded the calibration curve were diluted so that their florescence was within the calibration range. Samples were taken at the beginning and end of the experiment and at intervals in between and at the same time point as measurements of other parameters during the experiment. PAH (polycyclic aromatic hydrocarbons) and Total PAH extraction of the sample with dichloromethane. The samples are then purified using silica/alumina columns. analyzed. The analysis is performed with gas chromatography to separate the individual aromatic compounds and with a mass selective detector in the selected ion mode for identification and quantification. The parent PAH (e.g. naphthalene) and its alkylated homolog’s (e.g. C-1, C-2, C-3 and C-4) are also determined. These analyses can be used to distiquish between petroleum and combustion sources or a mixture of these sources. The individual PAH compounds and their alkylated homologs are reported.|EOE fluorescence analyses was carried out using a Horiba Scientific Aqualog Fluorometer. Total PAH were determined by gas chromatography (GC) with a flame ionization detector (GC/FID) and PAHs by GC with a mass selective detector [Short et al., 1996].|Time is in number of hours as indicated.||Short, J. W., T. J. Jackson, M. L. Larsen, and T. L. Wade (1996), Analytical methods used for the analysis of hydrocarbons in crude oil tissues, sediments, and seawater collected for the natural resources damage assessment of the Exxon Valdez oil spill, in Proceedings of the Exxon Valdez Oil Spill Symposium, edited by S. D. Rice et al., pp. 140–148, Am. Fish. Soc., Bethesda, Md. Wade, T. L., S. T. Sweet, J. N. Walpert, J. L. Sericano, J. J. Singer, and N. L. Guinasso Jr. (2011), Evaluation of possible inputs of oil from the Deepwater Horizon spill to the Loop Current and associated eddies in the Gulf of Mexico, in Monitoring and Modeling the Deepwater Horizon Oil Spill: A Record-Breaking Enterprise, Geophys. Monogr. Ser., doi:10.1029/2011GM 001095.
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Distribution info: Distribution: Distributor: Distributor: Distributor contact: Responsible party: Organisation name: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC) Contact info: Contact: Phone: Telephone: Voice: 3618253604 Address: Address: Delivery point: 6300 Ocean Drive City: Corpus Christi Administrative area: TX Postal code: 78412 Country: USA Electronic mail address: griidc@gomri.org Online Resource: Online Resource: Linkage: URL: https://data.gulfresearchinitiative.org Role: Role code: distributor Distributor format: Format: Name: csv Version: inapplicable File decompression technique: zip Distributor transfer options: Digital transfer options: Transfer size: 0.0052 Online: Online Resource: Linkage: URL: https://data.gulfresearchinitiative.org/data/R4.x263.000:0019 Protocol: https Name: Data Landing Page Description: GRIIDC dataset landing page Function: Online function code: information
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Metadata maintenance: Maintenance information: Maintenance and update frequency: unknown Maintenance note: This ISO metadata record was automatically generated from information provided to GRIIDC for dataset: R4.x263.000:0019 on 2019-08-20T18:07:52-05:00
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