Phytoplankton community response to crude oil and dispersants in four different mesocosm treatments after 72h

Aggregation and Degradation of Dispersants and Oil by Microbial Exopolymers (ADDOMEx)

DOI:
10.7266/N7028PMG
 
UDI:
R4.x263.000:0015
Last Update:
May 04 2017 20:42 UTC
 
Dataset Author(s):
Laura Bretherton, Manoj Kamalanathan, Jennifer Genzer, Jessica Hillhouse, Antonietta Quigg
Point of Contact:
Bretherton, Laura
Mount Allison University / Environmental Science
67 York St.
Sackville, New Brunswick  E4L 1E6
CAN
lmjbre@gmail.com
Funding Source:
RFP-IV
 
Extent Description:
Dataset contains laboratory observations of phytoplankton communities under a light microscope, natural seawater was collected from a coastal buoy in the Gulf of Mexico.

Identified Submitted In-Review Available
3 3 3 3

Suggested Citation:

Laura Bretherton, Manoj Kamalanathan, Jennifer Genzer, Jessica Hillhouse, Antonietta Quigg. 2017. Phytoplankton community response to crude oil and dispersants in four different mesocosm treatments after 72h. Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7028PMG

Abstract:

A mesocosm experiment was carried out using natural seawater from the Gulf of Mexico to look at how phytoplankton community composition changed in response to oil and dispersant exposure. The phytoplankton genera present in each tank was recorded after 72h of exposure using a light microscope.

Purpose:

Phytoplankton community structure can be profoundly altered in response to changes in environmental variables. This data was collected to assess how oil spills and chemical dispersants affect phytoplankton community ecology in the Gulf of Mexico.

Theme Keywords:

phytoplankton, community ecology, crude oil, dispersants, marine ecology, marine microbiology

File Format:

csv

Filename:

Phytoplankton Species Diversity for Mesocosm 4.csv (643 B)

Dataset Downloads:

2

Phytoplankton community response to crude oil and dispersants in four different mesocosm treatments after 72h



Identification Information
Distribution Information
Metadata Maintenance Information

Metadata: 
  File identifier: 
      R4.x263.000-0015-metadata.xml
  Language: 
      eng; USA
  Character set: 
    Character set code: 
      utf8
  Hierarchy level: 
    Scope code: 
      dataset
  Metadata author: 
    Responsible party: 
      Individual name: 
          Antonietta Quigg
      Organisation name: 
          Texas A&M University at Galveston / Marine Biology Department
      Position name: 
          Professor
      Contact info: 
        Contact: 
          Phone: 
            Telephone: 
              Voice: 
                  4097404990
              Facsimile: 
          Address: 
            Address: 
              Delivery point: 
                  P.O. Box 1675
              City: 
                  Galveston
              Administrative area: 
                  Texas
              Postal code: 
                  77553
              Country: 
                  USA
              Electronic mail address: 
                  quigga@tamug.edu
      Role: 
        Role code: 
          pointOfContact
  Date stamp: 
      2018-08-11T13:48:42+00:00
  Metadata standard name: 
      ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
  Metadata standard version: 
      ISO 19115-2:2009(E)
  Dataset URI: 
      https://data.gulfresearchinitiative.org/metadata/R4.x263.000:0015
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Identification info: Data identification: Citation: Citation: Title: Phytoplankton community response to crude oil and dispersants in four different mesocosm treatments after 72h Alternate title: Effects of oil and dispersant on phytoplankton diversity Date: Date: Date: 2017-05-04 Date type: Date type code: publication Identifier: Identifier: Code: Anchor: xlink: https://dx.doi.org/10.7266/N7028PMG title: DOI doi:10.7266/N7028PMG Abstract: A mesocosm experiment was carried out using natural seawater from the Gulf of Mexico to look at how phytoplankton community composition changed in response to oil and dispersant exposure. The phytoplankton genera present in each tank was recorded after 72h of exposure using a light microscope. Purpose: Phytoplankton community structure can be profoundly altered in response to changes in environmental variables. This data was collected to assess how oil spills and chemical dispersants affect phytoplankton community ecology in the Gulf of Mexico. Status: Progress code: completed Point of contact: Responsible party: Individual name: Laura Bretherton Organisation name: Mount Allison University / Environmental Science Position name: Postdoctoral Researcher Contact info: Contact: Phone: Telephone: Voice: Facsimile: Address: Address: Delivery point: 67 York St. City: Sackville Administrative area: New Brunswick Postal code: E4L 1E6 Country: CAN Electronic mail address: lmjbre@gmail.com Role: Role code: pointOfContact Point of contact: Responsible party: Individual name: Antonietta Quigg Organisation name: Texas A&M University at Galveston / Marine Biology Department Position name: Professor Contact info: Contact: Phone: Telephone: Voice: 4097404990 Facsimile: Address: Address: Delivery point: P.O. Box 1675 City: Galveston Administrative area: Texas Postal code: 77553 Country: USA Electronic mail address: quigga@tamug.edu Role: Role code: pointOfContact Descriptive keywords: Keywords: Keyword: phytoplankton Keyword: community ecology Keyword: crude oil Keyword: dispersants Keyword: marine ecology Keyword: marine microbiology Type: Keyword type code: theme Descriptive keywords: Keywords: Keyword: inapplicable Type: Keyword type code: place Resource constraints: title: Cite As Constraints: Use limitation: Laura Bretherton, Manoj Kamalanathan, Jennifer Genzer, Jessica Hillhouse, Antonietta Quigg. 2017. Phytoplankton community response to crude oil and dispersants in four different mesocosm treatments after 72h. Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7028PMG Resource constraints: title: CC0 License Legal constraints: Use constraints: Restriction code: licenceUnrestricted Other constraints: This information is released under the Creative Commons license - No Rights Reserved - CC0 1.0 Universal (https://creativecommons.org/publicdomain/zero/1.0/). The person who associated a work with this deed has dedicated the work to the public domain by waiving all of his or her rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law. You can copy, modify, distribute and perform the work, even for commercial purposes, all without asking permission. Resource constraints: title: Liability and Warranty Legal constraints: Other constraints: All materials on this website are made available to GRIIDC and in turn to you "as-is." Content may only be submitted by an individual who represents and warrants that s/he has sufficient rights to be able to make the content available under a CC0 waiver. There is no warranty (expressed or implied) to these materials, their title, accuracy, non-infringement of third party rights, or fitness for any particular purpose, including the performance or results you may obtain from their use. Use these materials at your own risk. Under no circumstances shall GRIIDC be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use or the inability to use either this website or the materials available via this website. If you are dissatisfied with any website feature, content, or terms of use, your sole and exclusive remedy is to discontinue use. Aggregation Info: AggregateInformation: Aggregate Data Set Name: title: Related Publication Citation Citation: Title: Bretherton, L., Kamalanathan, M., Genzer, J., Hillhouse, J., Setta, S., Liang, Y., … Quigg, A. (2019). Response of natural phytoplankton communities exposed to crude oil and chemical dispersants during a mesocosm experiment. Aquatic Toxicology, 206, 43–53. doi:10.1016/j.aquatox.2018.11.004 Date: inapplicable Aggregate Data Set Identifier: title: Related Publication DOI Identifier: Code: Anchor: xlink: https://dx.doi.org/10.1016/j.aquatox.2018.11.004 title: DOI doi:10.1016/j.aquatox.2018.11.004 Association Type: Association type code: crossReference Language: eng; USA Topic category: Topic category code: oceans Topic category: Topic category code: biota Topic category: Topic category code: environment Extent: Extent: Description: Dataset contains laboratory observations of phytoplankton communities under a light microscope, natural seawater was collected from a coastal buoy in the Gulf of Mexico. Supplemental Information: Treatment (CONTROL; WAF=Water Accommodated Fraction of oil; DCEWAF=Diluted Chemically Enhanced Water Accommodated Fraction of oil; CEWAF=Chemically Enhanced Water Accommodated Fraction of oil); Replicate (A, B, C; represent different replicate tanks); Chaetoceros (phytoplankton genus, 1=present, 0=absent); Coscinodiscus (phytoplankton genus, 1=present, 0=absent); Cylindrotheca (phytoplankton genus, 1=present, 0=absent); Lioloma (phytoplankton genus, 1=present, 0=absent); Navicula (phytoplankton genus, 1=present, 0=absent); Nitzschia (phytoplankton genus, 1=present, 0=absent); Pseudo-nitzschia (phytoplankton genus, 1=present, 0=absent); Skeletonema (phytoplankton genus, 1=present, 0=absent); Stephanopyxis (phytoplankton genus, 1=present, 0=absent); Synedra (phytoplankton genus, 1=present, 0=absent); Thalassionema (phytoplankton genus, 1=present, 0=absent); Thalassiosira (phytoplankton genus, 1=present, 0=absent)|Natural seawater was collected from a coastal buoy in the Gulf of Mexico and brought back to Texas A&M University at Galveston for manipulation. Treatments were prepared in specialized glass tanks called WAF makers using the natural seawater. WAF treatments were prepared by adding Macondo crude oil to a target concentration of 1mg L-1, and CEWAF treatments were made with a stock solution containing a 20:1 ratio of oil:Corexit. One third of the CEWAF was then diluted until the crude oil concentration was consistent with the WAF to make the DCEWAF treatments. Control treatments were not exposed to either oil or Corexit. The experiments were then set up in 100L glass tanks staged in a room fitted with fluorescent bulbs. All treatments were grown in triplicate with true biological replication. A 100mL sample was taken from each mesocosm tank after 72h and settled for 24h in an Utermohl chamber fitted with a 100mL cylinder. The plate was then removed to look at the sample under an inverted light microscope. All phytoplankton observed on the plate were identified to genus level using Tomas (1997). Representative photographs were taken using the attached camera and processed using Leica Application Suite (LAS) EZ v1.6.0. To calibrate the measurements recorded by LAS EZ software, a photograph of a micrometer was taken with the microscope’s camera and a known distance of 200µm was measured to determine the number of pixels (px) within that set distance. The resulting ratio (1px = 0.32µm) was used to calibrate measurements taken with the microscope and camera. Photographs can be found in GRIIDC dataset UDI: R4.x263.000:0017.|Utermohl chamber - Combined Plate Chamber, Hydro-Bios, Germany Inverted light microscope - Motic AE31, British Columbia, Canada|One 100mL sample per replicate tank at the end of the experiment (72h).||
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Distribution info: Distribution: Distributor: Distributor: Distributor contact: Responsible party: Organisation name: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC) Contact info: Contact: Phone: Telephone: Voice: 3618253604 Address: Address: Delivery point: 6300 Ocean Drive City: Corpus Christi Administrative area: TX Postal code: 78412 Country: USA Electronic mail address: griidc@gomri.org Online Resource: Online Resource: Linkage: URL: https://data.gulfresearchinitiative.org Role: Role code: distributor Distributor format: Format: Name: csv Version: inapplicable File decompression technique: Distributor transfer options: Digital transfer options: Transfer size: 0.0006 Online: Online Resource: Linkage: URL: https://data.gulfresearchinitiative.org/data/R4.x263.000:0015 Protocol: https Name: Data Landing Page Description: GRIIDC dataset landing page Function: Online function code: information
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Metadata maintenance: Maintenance information: Maintenance and update frequency: unknown Maintenance note: This ISO metadata record was automatically generated from information provided to GRIIDC for dataset: R4.x263.000:0015 on 2020-09-24T10:55:05-05:00
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