data

Metadata: 
  File identifier: 
      R4.x263.000-0013-metadata.xml
  Language: 
      eng; USA
  Character set: 
    Character set code: 
      utf8
  Hierarchy level: 
    Scope code: 
      dataset
  Metadata author: 
    Responsible party: 
      Individual name: 
          Uta Passow
      Organisation name: 
          University of California Santa Barbara / Marine Science Institute
      Position name: 
          Researcher
      Contact info: 
        Contact: 
          Phone: 
            Telephone: 
              Voice: 
                  8058932363
              Facsimile: 
          Address: 
            Address: 
              Delivery point: 
                  Bldg 520 Rm 4002 Fl 4L
              City: 
                  Santa Barbara
              Administrative area: 
                  California
              Postal code: 
                  93106
              Country: 
                  USA
              Electronic mail address: 
                  uta.passow@lifesci.ucsb.edu
      Role: 
        Role code: 
          pointOfContact
  Date stamp: 
      2018-08-11T13:39:11+00:00
  Metadata standard name: 
      ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
  Metadata standard version: 
      ISO 19115-2:2009(E)
  Dataset URI: 
      https://data.gulfresearchinitiative.org/metadata/R4.x263.000:0013
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  Identification info: 
    Data identification: 
      Citation: 
        Citation: 
          Title: 
              Impact of Corexit on aggregation of Skeletonema grethae in oil contaminated natural seawater, high oil 
              concentrations: Rolling table experiment to test the aggregation of diatoms, TEP(transparent 
              exopolymers), POC (particulate organic carbon) as a function of the presence of Corexit and investigate 
              the change in marine snow over time
          Alternate title: 
              Impact of Corexit on aggregation of Skeletonema grethae in oil contaminated natural seawater, high oil 
              concentrations
          Date: 
            Date: 
              Date: 
                  2017-03-29
              Date type: 
                Date type code: 
                  revision
      Abstract: 
          Testing the direct effect of Corexit when added to oil contaminated natural seawater on aggregation of 
          diatoms. Once aggregates had formed samples were collected separately from the surrounding seawater and the 
          marine snow fraction.
      Purpose: 
          This is an experiment testing the impact of Corexit on aggregation of Skeletonema grethae in naturally 
          dispersed oil (NWAF) after the Refugio Oil spill off California May 2015. The NWAF was collected 5/21/2015 
          less than 2 days after the spill. Roller tank experiments mimic in situ conditions of the ocean in that 
          continuous sinking of particles is possible (infinity water column). Once aggregates > 1mm (operationally 
          defined) have formed, they are harvested separately from the surrounding seawater, which contains 
          non-aggregates cells or small aggregates. Both fractions are analyzed separately, so that the fraction of 
          material incorporated within aggregates may be calculated. In this experiment aggregation of the diatom 
          Skeletonema grethae and natural particles was monitored in two treatments: NWAF+Skel treatment contained the 
          diatom culture and natural seawater that had been contaminated with oil. The NWAF+Skel+Cor treatment 
          additionally contained Corexit (50 µL per liter sample). Samples were analysed for cell abundance, 
          particulate organic carbon (POC) and nitrogen, and transparent exopolymer particles (TEP).
      Status: 
        Progress code: 
          completed
      Point of contact: 
        Responsible party: 
          Individual name: 
              Uta Passow
          Organisation name: 
              University of California Santa Barbara / Marine Science Institute
          Position name: 
              Researcher
          Contact info: 
            Contact: 
              Phone: 
                Telephone: 
                  Voice: 
                      8058932363
                  Facsimile: 
              Address: 
                Address: 
                  Delivery point: 
                      Bldg 520 Rm 4002 Fl 4L
                  City: 
                      Santa Barbara
                  Administrative area: 
                      California
                  Postal code: 
                      93106
                  Country: 
                      USA
                  Electronic mail address: 
                      uta.passow@lifesci.ucsb.edu
          Role: 
            Role code: 
              pointOfContact
      Descriptive keywords: 
        Keywords: 
          Keyword: 
              marine snow
          Keyword: 
              marine oil snow
          Keyword: 
              Corexit
          Keyword: 
              oil
          Keyword: 
              TEP
          Keyword: 
              Skeletonema grethae
          Type: 
            Keyword type code: 
              theme
      Descriptive keywords: 
        Keywords: 
          Keyword: 
            inapplicable
          Type: 
            Keyword type code: 
              place
      Language: 
          eng; USA
      Topic category: 
        Topic category code: 
          oceans
      Topic category: 
        Topic category code: 
          biota
      Topic category: 
        Topic category code: 
          environment
      Extent: 
        Extent: 
          Description: 
              Dataset contains laboratory measurements of interactions of oil and Corexit on diatom species, no field 
              sampling involved.
      Supplemental Information: 
          Date (MM/DD/YYYY), Day, Tank, Treatment, Fraction, Replicate particulate organic carbon = POC (ug/tank) C:N – 
          weight ratio of POC to PON transparent exopolymer particles = TEP Gum Xanthan equivalent = GXeq aggregates > 
          1 mm = agg. cells/tank Average = avg Standard deviation (of 6 replicate counts) = std NWAF+Skel treatment 
          contained the diatom culture and natural seawater that had been contaminated with oil NWAF+Skel+Cor-50 = 
          treatment with natural accommodated oil fraction plus Skeletonema plus 50µL Corexit per liter 
          sample|Replicate acrylic roller tanks of 1.16 L volume were filled bubble free. Experiments were incubated 
          in the dark, at 15˚C at an rpm of 1.4. Experiments were conducted with oil-contaminated seawater (NWAF) 
          collected by bucket from the surface near Santa Barbara, CA (34˚27 N, 120˚03 W) 1.5 days after the Refugio 
          pipeline spill. Skeletonema grethae (CCMP 775) was added to replicate treatments prepared for each 
          experiment: The oil-contaminated seawater with S. grethae (in early stationary phase) was compared to the 
          respective treatments where 50 µL Corexit per liter was added. The oil-contaminated seawater was siphoned 
          into roller tanks from below the bucket surface. Aggregate appearance and numbers were monitored during the 
          experiment. When sufficient aggregates had formed, all treatments were harvested. Aggregates > 1mm (visually 
          discernable) were manually collected and analyzed. After all aggregates > 1 mm were removed the remainder of 
          the material, termed surrounding seawater (SSW), was subsampled. Both fractions were analyzed for POC, cell 
          abundance, TEP (transparent exopolymer particles). Duplicate filters (GF/F) prepared for POC analysis were 
          measured in a CEC44OHA elemental analyzer (Control equipment). Diatom cells were counted (Olympus CX41) 
          using a hemocytometer; at least 6 subsamples and 200 cells each were counted per sample. Counts were at 
          times only conducted on 1 of the two replicate treatments. TEP concentrations were determined in triplicate 
          using the colorimetric method and are expressed in Gum Xanthan equivalents (GXeq.). Corexit binds to the dye 
          Alcian Blue generating artificially high “TEP” values in the presence of Corexit, thus TEP concentrations 
          can’t be determined in the presence of Corexit. All biomass results (cell abundance, POC, TEP concentration) 
          are normalized per tank to allow budgets and make aggregate and SSW fractions directly comparable. 
          ||micrometer to decimeter, days||
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  Distribution info: 
    Distribution: 
      Distributor: 
        Distributor: 
          Distributor contact: 
            Responsible party: 
              Organisation name: 
                  Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC)
              Contact info: 
                Contact: 
                  Phone: 
                    Telephone: 
                      Voice: 
                          3618253604
                  Address: 
                    Address: 
                      Delivery point: 
                          6300 Ocean Drive
                      City: 
                          Corpus Christi
                      Administrative area: 
                          TX
                      Postal code: 
                          78412
                      Country: 
                          USA
                      Electronic mail address: 
                          griidc@gomri.org
                  Online Resource: 
                    Online Resource: 
                      Linkage: 
                        URL: 
                          https://data.gulfresearchinitiative.org
              Role: 
                Role code: 
                  distributor
          Distributor format: 
            Format: 
              Name: 
                  csv
              Version: 
                inapplicable
              File decompression technique: 
          Distributor transfer options: 
            Digital transfer options: 
              Transfer size: 
                  0.002
              Online: 
                Online Resource: 
                  Linkage: 
                    URL: 
                      https://data.gulfresearchinitiative.org/data/R4.x263.000:0013
                  Protocol: 
                      https
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  Metadata maintenance: 
    Maintenance information: 
      Maintenance and update frequency: 
        unknown
      Maintenance note: 
          This ISO metadata record was automatically generated from information provided to GRIIDC for dataset: 
          R4.x263.000:0013 on 2019-02-17T13:44:55+00:00
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