Full View
Normal View
|
Impact of Corexit on aggregation of Skeletonema grethae in oil contaminated natural seawater, high oil concentrations: Rolling table experiment to test the aggregation of diatoms, TEP(transparent exopolymers), POC (particulate organic carbon) as a function of the presence of Corexit and investigate the change in marine snow over timeAggregation and Degradation of Dispersants and Oil by Microbial Exopolymers (ADDOMEx)
|
Suggested Citation:
Uta Passow (2017) Impact of Corexit on aggregation of Skeletonema grethae in oil contaminated natural seawater, high oil concentrations: Rolling table experiment to test the aggregation of diatoms, TEP(transparent exopolymers), POC (particulate organic carbon) as a function of the presence of Corexit and investigate the change in marine snow over time. Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University-Corpus Christi. doi:10.7266/N7125QQD
Abstract:
Testing the direct effect of Corexit when added to oil contaminated natural seawater on aggregation of diatoms. Once aggregates had formed samples were collected separately from the surrounding seawater and the marine snow fraction.
Purpose:
This is an experiment testing the impact of Corexit on aggregation of Skeletonema grethae in naturally dispersed oil (NWAF) after the Refugio Oil spill off California May 2015. The NWAF was collected 5/21/2015 less than 2 days after the spill. Roller tank experiments mimic in situ conditions of the ocean in that continuous sinking of particles is possible (infinity water column). Once aggregates > 1mm (operationally defined) have formed, they are harvested separately from the surrounding seawater, which contains non-aggregates cells or small aggregates. Both fractions are analyzed separately, so that the fraction of material incorporated within aggregates may be calculated. In this experiment aggregation of the diatom Skeletonema grethae and natural particles was monitored in two treatments: NWAF+Skel treatment contained the diatom culture and natural seawater that had been contaminated with oil. The NWAF+Skel+Cor treatment additionally contained Corexit (50 µL per liter sample). Samples were analysed for cell abundance, particulate organic carbon (POC) and nitrogen, and transparent exopolymer particles (TEP).
Theme Keywords:
marine snow, marine oil snow, Corexit, oil, TEP, Skeletonema grethae
File Format:
csv
Filename:
DIF_Rx263_000_0013-2.csv (2.02 KB)
Dataset Downloads:
0
Impact of Corexit on aggregation of Skeletonema grethae in oil contaminated natural seawater, high oil concentrations: Rolling table experiment to test the aggregation of diatoms, TEP(transparent exopolymers), POC (particulate organic carbon) as a function of the presence of Corexit and investigate the change in marine snow over time
Identification Information
Distribution Information
Metadata Maintenance Information
Metadata: File identifier: R4.x263.000-0013-metadata.xml Language: eng; USA Character set: Character set code: utf8 Hierarchy level: Scope code: dataset Metadata author: Responsible party: Individual name: Uta Passow Organisation name: University of California Santa Barbara / Marine Science Institute Position name: Researcher Contact info: Contact: Phone: Telephone: Voice: 8058932363 Facsimile: Address: Address: Delivery point: Bldg 520 Rm 4002 Fl 4L City: Santa Barbara Administrative area: California Postal code: 93106 Country: USA Electronic mail address: uta.passow@lifesci.ucsb.edu Role: Role code: pointOfContact Date stamp: 2018-08-11T13:39:11+00:00 Metadata standard name: ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data Metadata standard version: ISO 19115-2:2009(E) Dataset URI: https://data.gulfresearchinitiative.org/metadata/R4.x263.000:0013Return To Index
Identification info: Data identification: Citation: Citation: Title: Impact of Corexit on aggregation of Skeletonema grethae in oil contaminated natural seawater, high oil concentrations: Rolling table experiment to test the aggregation of diatoms, TEP(transparent exopolymers), POC (particulate organic carbon) as a function of the presence of Corexit and investigate the change in marine snow over time Alternate title: Impact of Corexit on aggregation of Skeletonema grethae in oil contaminated natural seawater, high oil concentrations Date: Date: Date: 2017-03-29 Date type: Date type code: revision Abstract: Testing the direct effect of Corexit when added to oil contaminated natural seawater on aggregation of diatoms. Once aggregates had formed samples were collected separately from the surrounding seawater and the marine snow fraction. Purpose: This is an experiment testing the impact of Corexit on aggregation of Skeletonema grethae in naturally dispersed oil (NWAF) after the Refugio Oil spill off California May 2015. The NWAF was collected 5/21/2015 less than 2 days after the spill. Roller tank experiments mimic in situ conditions of the ocean in that continuous sinking of particles is possible (infinity water column). Once aggregates > 1mm (operationally defined) have formed, they are harvested separately from the surrounding seawater, which contains non-aggregates cells or small aggregates. Both fractions are analyzed separately, so that the fraction of material incorporated within aggregates may be calculated. In this experiment aggregation of the diatom Skeletonema grethae and natural particles was monitored in two treatments: NWAF+Skel treatment contained the diatom culture and natural seawater that had been contaminated with oil. The NWAF+Skel+Cor treatment additionally contained Corexit (50 µL per liter sample). Samples were analysed for cell abundance, particulate organic carbon (POC) and nitrogen, and transparent exopolymer particles (TEP). Status: Progress code: completed Point of contact: Responsible party: Individual name: Uta Passow Organisation name: University of California Santa Barbara / Marine Science Institute Position name: Researcher Contact info: Contact: Phone: Telephone: Voice: 8058932363 Facsimile: Address: Address: Delivery point: Bldg 520 Rm 4002 Fl 4L City: Santa Barbara Administrative area: California Postal code: 93106 Country: USA Electronic mail address: uta.passow@lifesci.ucsb.edu Role: Role code: pointOfContact Descriptive keywords: Keywords: Keyword: marine snow Keyword: marine oil snow Keyword: Corexit Keyword: oil Keyword: TEP Keyword: Skeletonema grethae Type: Keyword type code: theme Descriptive keywords: Keywords: Keyword: inapplicable Type: Keyword type code: place Language: eng; USA Topic category: Topic category code: oceans Topic category: Topic category code: biota Topic category: Topic category code: environment Extent: Extent: Description: Dataset contains laboratory measurements of interactions of oil and Corexit on diatom species, no field sampling involved. Supplemental Information: Date (MM/DD/YYYY), Day, Tank, Treatment, Fraction, Replicate particulate organic carbon = POC (ug/tank) C:N – weight ratio of POC to PON transparent exopolymer particles = TEP Gum Xanthan equivalent = GXeq aggregates > 1 mm = agg. cells/tank Average = avg Standard deviation (of 6 replicate counts) = std NWAF+Skel treatment contained the diatom culture and natural seawater that had been contaminated with oil NWAF+Skel+Cor-50 = treatment with natural accommodated oil fraction plus Skeletonema plus 50µL Corexit per liter sample|Replicate acrylic roller tanks of 1.16 L volume were filled bubble free. Experiments were incubated in the dark, at 15˚C at an rpm of 1.4. Experiments were conducted with oil-contaminated seawater (NWAF) collected by bucket from the surface near Santa Barbara, CA (34˚27 N, 120˚03 W) 1.5 days after the Refugio pipeline spill. Skeletonema grethae (CCMP 775) was added to replicate treatments prepared for each experiment: The oil-contaminated seawater with S. grethae (in early stationary phase) was compared to the respective treatments where 50 µL Corexit per liter was added. The oil-contaminated seawater was siphoned into roller tanks from below the bucket surface. Aggregate appearance and numbers were monitored during the experiment. When sufficient aggregates had formed, all treatments were harvested. Aggregates > 1mm (visually discernable) were manually collected and analyzed. After all aggregates > 1 mm were removed the remainder of the material, termed surrounding seawater (SSW), was subsampled. Both fractions were analyzed for POC, cell abundance, TEP (transparent exopolymer particles). Duplicate filters (GF/F) prepared for POC analysis were measured in a CEC44OHA elemental analyzer (Control equipment). Diatom cells were counted (Olympus CX41) using a hemocytometer; at least 6 subsamples and 200 cells each were counted per sample. Counts were at times only conducted on 1 of the two replicate treatments. TEP concentrations were determined in triplicate using the colorimetric method and are expressed in Gum Xanthan equivalents (GXeq.). Corexit binds to the dye Alcian Blue generating artificially high “TEP” values in the presence of Corexit, thus TEP concentrations can’t be determined in the presence of Corexit. All biomass results (cell abundance, POC, TEP concentration) are normalized per tank to allow budgets and make aggregate and SSW fractions directly comparable. ||micrometer to decimeter, days||Return To Index
Distribution info: Distribution: Distributor: Distributor: Distributor contact: Responsible party: Organisation name: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC) Contact info: Contact: Phone: Telephone: Voice: 3618253604 Address: Address: Delivery point: 6300 Ocean Drive City: Corpus Christi Administrative area: TX Postal code: 78412 Country: USA Electronic mail address: griidc@gomri.org Online Resource: Online Resource: Linkage: URL: https://data.gulfresearchinitiative.org Role: Role code: distributor Distributor format: Format: Name: csv Version: inapplicable File decompression technique: Distributor transfer options: Digital transfer options: Transfer size: 0.002 Online: Online Resource: Linkage: URL: https://data.gulfresearchinitiative.org/data/R4.x263.000:0013 Protocol: httpsReturn To Index
Metadata maintenance: Maintenance information: Maintenance and update frequency: unknown Maintenance note: This ISO metadata record was automatically generated from information provided to GRIIDC for dataset: R4.x263.000:0013 on 2019-02-17T13:44:55+00:00Return To Index