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Impact of Corexit on aggregation of old Chaetoceros: Rolling table experiment to test the aggregation of diatoms, TEP(transparent exopolymers), POC(particulate organic carbon) as a function of oil and oil+Corexit

Aggregation and Degradation of Dispersants and Oil by Microbial Exopolymers (ADDOMEx)

DOI:
10.7266/N74T6GDC
 
UDI:
R4.x263.000:0012
Last Update:
Aug 02 2017 19:14 UTC
 
Dataset Author(s):
Uta Passow
Point of Contact:
Passow, Uta
University of California Santa Barbara / Marine Science Institute
Bldg 520 Rm 4002 Fl 4L
Santa Barbara, California  93106
USA
uta.passow@lifesci.ucsb.edu
Funding Source:
RFP-IV
 
Extent Description:
Dataset contains laboratory measurements interactions of oil and Corexit with diatom species, no field sampling involved

Identified Submitted Metadata Available
3 3 3 3

Abstract:

Testing the direct effect of oil or oil+Corexit on aggregation of diatoms. Once aggregates had formed samples were collected separately from the surrounding seawater and the marine snow fraction. In contrast to the earlier experiment cells were older and initial TEP concentration higher.

Purpose:

This is one experiment of two investigating the effects of oil and oil+Corexit on the formation of diatoms aggregates. Roller tank experiments mimic in situ conditions of the ocean in that continuous sinking of particles is possible (infinity water column). Once aggregates > 1mm (operationally defined) have formed, they are harvested separately from the surrounding seawater, which contains non-aggregates cells or small aggregates. Both fractions are analyzed separately, so that the fraction of material incorporated within aggregates may be calculated. In this experiment Chaetoceros in early stationary phase was used, and three treatments analyzed: the control that contained only the culture, the OIL treatment that contained 1 mL oil per L culture and the Oil+Corexit treatment, which additionally contained Corexit. Samples were analysed for cell abundance, particulate organic carbon (POC), PO13C and transparent exopolymer particles (TEP).

Theme Keywords:

Chaetoceros, marine snow, marine oil snow, Corexit, oil, TEP, 13C

File Format:

csv

Dataset Downloads:

1

Impact of Corexit on aggregation of old Chaetoceros: Rolling table experiment to test the aggregation of diatoms, TEP(transparent exopolymers), POC(particulate organic carbon) as a function of oil and oil+Corexit



Identification Information
Distribution Information
Metadata Maintenance Information

Metadata: 
  File identifier: 
      R4.x263.000-0012-metadata.xml
  Language: 
      eng; USA
  Character set: 
    Character set code: 
      utf8
  Hierarchy level: 
    Scope code: 
      dataset
  Metadata author: 
    Responsible party: 
      Individual name: 
          Uta Passow
      Organisation name: 
          University of California Santa Barbara / Marine Science Institute
      Position name: 
          Researcher
      Contact info: 
        Contact: 
          Phone: 
            Telephone: 
              Voice: 
                  8058932363
              Facsimile: 
          Address: 
            Address: 
              Delivery point: 
                  Bldg 520 Rm 4002 Fl 4L
              City: 
                  Santa Barbara
              Administrative area: 
                  California
              Postal code: 
                  93106
              Country: 
                  USA
              Electronic mail address: 
                  uta.passow@lifesci.ucsb.edu
      Role: 
        Role code: 
          pointOfContact
  Date stamp: 
      2017-05-09T21:07:31+00:00
  Metadata standard name: 
      ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
  Metadata standard version: 
      ISO 19115-2:2009(E)
  Dataset URI: 
      https://data.gulfresearchinitiative.org/metadata/R4.x263.000:0012
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Identification info: Data identification: Citation: Citation: Title: Impact of Corexit on aggregation of old Chaetoceros: Rolling table experiment to test the aggregation of diatoms, TEP(transparent exopolymers), POC(particulate organic carbon) as a function of oil and oil+Corexit Alternate title: Impact of Corexit on aggregation of old Chaetoceros Date: Date: Date: 2017-03-29 Date type: Date type code: revision Abstract: Testing the direct effect of oil or oil+Corexit on aggregation of diatoms. Once aggregates had formed samples were collected separately from the surrounding seawater and the marine snow fraction. In contrast to the earlier experiment cells were older and initial TEP concentration higher. Purpose: This is one experiment of two investigating the effects of oil and oil+Corexit on the formation of diatoms aggregates. Roller tank experiments mimic in situ conditions of the ocean in that continuous sinking of particles is possible (infinity water column). Once aggregates > 1mm (operationally defined) have formed, they are harvested separately from the surrounding seawater, which contains non-aggregates cells or small aggregates. Both fractions are analyzed separately, so that the fraction of material incorporated within aggregates may be calculated. In this experiment Chaetoceros in early stationary phase was used, and three treatments analyzed: the control that contained only the culture, the OIL treatment that contained 1 mL oil per L culture and the Oil+Corexit treatment, which additionally contained Corexit. Samples were analysed for cell abundance, particulate organic carbon (POC), PO13C and transparent exopolymer particles (TEP). Status: Progress code: completed Point of contact: Responsible party: Individual name: Uta Passow Organisation name: University of California Santa Barbara / Marine Science Institute Position name: Researcher Contact info: Contact: Phone: Telephone: Voice: 8058932363 Facsimile: Address: Address: Delivery point: Bldg 520 Rm 4002 Fl 4L City: Santa Barbara Administrative area: California Postal code: 93106 Country: USA Electronic mail address: uta.passow@lifesci.ucsb.edu Role: Role code: pointOfContact Descriptive keywords: Keywords: Keyword: Chaetoceros Keyword: marine snow Keyword: marine oil snow Keyword: Corexit Keyword: oil Keyword: TEP Keyword: 13C Type: Keyword type code: theme Descriptive keywords: Keywords: Keyword: inapplicable Type: Keyword type code: place Language: eng; USA Topic category: Topic category code: oceans Topic category: Topic category code: biota Topic category: Topic category code: environment Extent: Extent: Description: Dataset contains laboratory measurements interactions of oil and Corexit with diatom species, no field sampling involved Supplemental Information: tank number replicate cells/tank PO13C particulate organic carbon = POC (ug/tank) particulate organic nitrogen = PON transparent exopolymer particles = TEP µg Gum Xanthan equivalent/tank or µg Xeq/tank aggregates > 1 mm = agg. Surrounding seawater = SSW The ratio between POC and PON per weight = C:N Control treatment = Contr Treatment with oil (1 ml oil per Liter) = OIL Treatment with oil and Corexit (30µL per mL oil) = oil+Cor-30 Replicates = repl Average = avg Standard deviation = std The fraction of organic carbon due to cells = Cell-C The fraction of organic carbon due to oil or oil + corexit = Oil+cor-C| Replicate acrylic roller tanks of 1.16 L volume were filled bubble free. Experiments were incubated in the dark, at 20˚C at an rpm of 2.4 (1 & 2). Experiments were conducted with Macondo surrogate oil and Chaetoceros sp., (isolated Nov. 2014 at 38.70°N, 123.67°W) a common bloom forming diatom. Three types of treatments, each in duplicate, were compared: a control (diatom culture), an oil addition (OIL) and an oil plus Corexit addition (Oil+Cor). To prepare the OIL treatments, one mL of Macondo oil (Marlin Platform, Dorado source oil) was slowly added into the center of the turning tank, using a syringe inserted through the silicon stopper. Corexit 9500A (Clean Seas), 30 µL, was added for the oil plus Corexit treatments (DOR: 1: 33 or 1: 10). Upon addition of source oil, oil droplets rose to the top, and especially in the absence of Corexit, a slick layer formed, which was not available for aggregation and was not sampled. Treatments of experiment Control: 1.16L Chaetoceros OIL (Chemically undispersed oil): 1.16L Chaetoceros + 1 mL source oil
          Oil+Cor-30 (Corexit-dispersed oil):	1.16L Chaetoceros + 1 mL source oil + 30 µL Corexit
          
Aggregate appearance and numbers were monitored during all experiments. When sufficient aggregates had formed, all treatments were harvested. Aggregates > 1mm (visually discernable) were manually collected and analyzed. After all aggregates > 1 mm were removed the remainder of the material, termed surrounding seawater (SSW), was subsampled. Both fractions were analyzed for POC, cell abundance, TEP (transparent exopolymer particles), and PO13C. Dispersed oil concentrations and partitioning between the aggregate and SSW phase was determined using POC and PO13C data. PO13C of the diatoms, the oil and the Corexit were separately determined (quadruplicates) and a two endmember mass balance calculation used to estimate the relative contributions of (i) cells and (ii) oil or oil+corexit to the particulate organic carbon (POC). Duplicate filters (GF/F) prepared for POC analysis were measured in a CEC44OHA elemental analyzer (Control equipment). The PO13C signature was determined on replicate filters using a Finnigan Delta Plus Advantage. Diatom cells were counted (Olympus CX41) using a hemocytometer; at least 6 subsamples and 200 cells each were counted per sample. Counts were at times only conducted on 1 of the two replicate treatments. TEP concentrations were determined in triplicate using the colorimetric method and are expressed in Gum Xanthan equivalents (GXeq.). Corexit binds to the dye Alcian Blue generating artificially high “TEP” values in the presence of Corexit, thus TEP concentrations can’t be determined in the presence of Corexit. All biomass results (cell abundance, POC, TEP concentration) are normalized per tank to allow budgets and make aggregate and SSW fractions directly comparable.||micrometer to decimeter, days||
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Distribution info: Distribution: Distributor: Distributor: Distributor contact: Responsible party: Organisation name: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC) Contact info: Contact: Phone: Telephone: Voice: +1-361-825-3604 Facsimile: +1-361-825-2050 Address: Address: Delivery point: 6300 Ocean Drive Unit 5869 City: Corpus Christi Administrative area: Texas Postal code: 78412-5869 Country: USA Electronic mail address: griidc@gomri.org Online Resource: Online Resource: Linkage: URL: https://data.gulfresearchinitiative.org Role: Role code: distributor Distributor format: Format: Name: csv Version: inapplicable File decompression technique: Distributor transfer options: Digital transfer options: Transfer size: 0.0021 Online: Online Resource: Linkage: URL: https://data.gulfresearchinitiative.org/data/R4.x263.000:0012 Protocol: https
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Metadata maintenance: Maintenance information: Maintenance and update frequency: unknown Maintenance note: This ISO metadata record was automatically generated from information provided to GRIIDC for dataset: R4.x263.000:0012 on 2017-03-29T19:15:40+00:00
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