GOM coastal waters (GOMCOAST): Production of TEP (transparent exopolymers) and CSP (Coomassie stained particles) as a function of oil

Aggregation and Degradation of Dispersants and Oil by Microbial Exopolymers (ADDOMEx)

DOI:
10.7266/N7RV0M1R
 
UDI:
R4.x263.000:0010
Last Update:
Aug 30 2017 21:11 UTC
 
Dataset Author(s):
Uta Passow
Point of Contact:
Passow, Uta
University of California Santa Barbara / Marine Science Institute
Bldg 520 Rm 4002 Fl 4L
Santa Barbara, California  93106
USA
uta.passow@lifesci.ucsb.edu
Funding Source:
RFP-IV
 
Extent Description:
Dataset contains laboratory measurements of TEP and CSP, seawater was collected 16 July 2016 close to TABS buoy R (29˚38.1000’N, 93˚38.5020’W) in the Gulf of Mexico.

Identified Submitted In-Review Available
3 3 3 3

Suggested Citation:

Uta Passow. 2017. GOM coastal waters (GOMCOAST): Production of TEP (transparent exopolymers) and CSP (Coomassie stained particles) as a function of oil. Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7RV0M1R

Abstract:

Testing the effect WAF (water accomodated fraction) on TEP and CSP. Samples were collected as a time series in the Control, WAF, and the WAF silicon tube treatment. Qualitative, but not quantitative samples were also collected from the sea surface microlayer and the sunken marine snow.

Purpose:

This mesocosm experiment investigated the response of coastal plankton community from the Gulf of Mexico to dispersed oil in comparison to conditions without oil. Specifically, the effect of oil addition for the production of transparent exopolymer particles (TEP) and CSP (coomassie stainable particles) was tested. TEP, that are rich in polysaccharides, are central for the formation of marine snow. The role of CSP, which are protein-rich is less clear.

Theme Keywords:

TEP, oil, WAF, CSP

File Format:

csv

Filename:

R4.x263.000 0010.csv (1.15 KB)

Dataset Downloads:

6

GOM coastal waters (GOMCOAST): Production of TEP (transparent exopolymers) and CSP (Coomassie stained particles) as a function of oil



Identification Information
Distribution Information
Metadata Maintenance Information

Metadata: 
  File identifier: 
      R4.x263.000-0010-metadata.xml
  Language: 
      eng; USA
  Character set: 
    Character set code: 
      utf8
  Hierarchy level: 
    Scope code: 
      dataset
  Metadata author: 
    Responsible party: 
      Individual name: 
          Kathleen (Kathy) Schwehr
      Organisation name: 
          Texas A&M University at Galveston / Marine Sciences Department
      Position name: 
          Associate Research Scientist
      Contact info: 
        Contact: 
          Phone: 
            Telephone: 
              Voice: 
                  4097404452
              Facsimile: 
          Address: 
            Address: 
              Delivery point: 
                  P.O. Box 1675
              City: 
                  Galveston
              Administrative area: 
                  Texas
              Postal code: 
                  77553
              Country: 
                  USA
              Electronic mail address: 
                  schwehrk@tamug.edu
      Role: 
        Role code: 
          pointOfContact
  Date stamp: 
      2018-08-11T13:40:28+00:00
  Metadata standard name: 
      ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
  Metadata standard version: 
      ISO 19115-2:2009(E)
  Dataset URI: 
      https://data.gulfresearchinitiative.org/metadata/R4.x263.000:0010
Return To Index

Identification info: Data identification: Citation: Citation: Title: GOM coastal waters (GOMCOAST): Production of TEP (transparent exopolymers) and CSP (Coomassie stained particles) as a function of oil Alternate title: TEP (transparent exopolymers) and CSP (Coomassie stained particles) as a function of oil Date: Date: Date: 2017-08-30 Date type: Date type code: publication Identifier: Identifier: Code: Anchor: xlink: https://dx.doi.org/10.7266/N7RV0M1R title: DOI doi:10.7266/N7RV0M1R Abstract: Testing the effect WAF (water accomodated fraction) on TEP and CSP. Samples were collected as a time series in the Control, WAF, and the WAF silicon tube treatment. Qualitative, but not quantitative samples were also collected from the sea surface microlayer and the sunken marine snow. Purpose: This mesocosm experiment investigated the response of coastal plankton community from the Gulf of Mexico to dispersed oil in comparison to conditions without oil. Specifically, the effect of oil addition for the production of transparent exopolymer particles (TEP) and CSP (coomassie stainable particles) was tested. TEP, that are rich in polysaccharides, are central for the formation of marine snow. The role of CSP, which are protein-rich is less clear. Status: Progress code: completed Point of contact: Responsible party: Individual name: Uta Passow Organisation name: University of California Santa Barbara / Marine Science Institute Position name: Researcher Contact info: Contact: Phone: Telephone: Voice: 8058932363 Facsimile: Address: Address: Delivery point: Bldg 520 Rm 4002 Fl 4L City: Santa Barbara Administrative area: California Postal code: 93106 Country: USA Electronic mail address: uta.passow@lifesci.ucsb.edu Role: Role code: pointOfContact Descriptive keywords: Keywords: Keyword: TEP Keyword: oil Keyword: WAF Keyword: CSP Type: Keyword type code: theme Descriptive keywords: Keywords: Keyword: inapplicable Type: Keyword type code: place Resource constraints: title: Cite As Constraints: Use limitation: Uta Passow. 2017. GOM coastal waters (GOMCOAST): Production of TEP (transparent exopolymers) and CSP (Coomassie stained particles) as a function of oil. Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7RV0M1R Resource constraints: title: CC0 License Legal constraints: Use constraints: Restriction code: licenceUnrestricted Other constraints: This information is released under the Creative Commons license - No Rights Reserved - CC0 1.0 Universal (https://creativecommons.org/publicdomain/zero/1.0/). The person who associated a work with this deed has dedicated the work to the public domain by waiving all of his or her rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law. You can copy, modify, distribute and perform the work, even for commercial purposes, all without asking permission. Resource constraints: title: Liability and Warranty Legal constraints: Other constraints: All materials on this website are made available to GRIIDC and in turn to you "as-is." Content may only be submitted by an individual who represents and warrants that s/he has sufficient rights to be able to make the content available under a CC0 waiver. There is no warranty (expressed or implied) to these materials, their title, accuracy, non-infringement of third party rights, or fitness for any particular purpose, including the performance or results you may obtain from their use. Use these materials at your own risk. Under no circumstances shall GRIIDC be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use or the inability to use either this website or the materials available via this website. If you are dissatisfied with any website feature, content, or terms of use, your sole and exclusive remedy is to discontinue use. Aggregation Info: AggregateInformation: Aggregate Data Set Name: title: Related Publication Citation Citation: Title: Bera, G., Doyle, S., Passow, U., Kamalanathan, M., Wade, T. L., Sylvan, J. B., … Knap, A. H. (2019). Biological response to dissolved versus dispersed oil. Marine Pollution Bulletin, 110713. doi:10.1016/j.marpolbul.2019.110713 Date: inapplicable Aggregate Data Set Identifier: title: Related Publication DOI Identifier: Code: Anchor: xlink: https://dx.doi.org/10.1016/j.marpolbul.2019.110713 title: DOI doi:10.1016/j.marpolbul.2019.110713 Association Type: Association type code: crossReference Aggregation Info: AggregateInformation: Aggregate Data Set Name: title: Related Publication Citation Citation: Title: Bretherton, L., Kamalanathan, M., Genzer, J., Hillhouse, J., Setta, S., Liang, Y., … Quigg, A. (2019). Response of natural phytoplankton communities exposed to crude oil and chemical dispersants during a mesocosm experiment. Aquatic Toxicology, 206, 43–53. doi:10.1016/j.aquatox.2018.11.004 Date: inapplicable Aggregate Data Set Identifier: title: Related Publication DOI Identifier: Code: Anchor: xlink: https://dx.doi.org/10.1016/j.aquatox.2018.11.004 title: DOI doi:10.1016/j.aquatox.2018.11.004 Association Type: Association type code: crossReference Language: eng; USA Topic category: Topic category code: oceans Topic category: Topic category code: biota Topic category: Topic category code: environment Extent: Extent: Description: Dataset contains laboratory measurements of TEP and CSP, seawater was collected 16 July 2016 close to TABS buoy R (29˚38.1000’N, 93˚38.5020’W) in the Gulf of Mexico. Supplemental Information: Date (MM/DD/YYYY) Sample time (HH:MM) Timepoint (hrs) SSW = surrounding seawater, sample does not include marine snow; Avg = average (of three replicate mesocosms); Stdev = standard deviation; TEP = Transparent Exopolymer Particles (ug GXeq/L); CSP = Coomassie Blue Particles (mg BSAeq/L); GXeq = Gum Xanthan equivalent; BSA eq = Bovine serum albumin equivalent; Control = Control treatment containing seawater and plankton community; WAF = Water accommodated fraction treatment, containing WAF made from the seawater (see methods) and plankton community; |In order to create an environment for controlled testing of TEP and CSP formation six 112 L capacity glass tanks (40x40x72 cm3) were used as mesocosms. Three replicate tanks were considered controls and three oil treatments, produced with a water accommodated fraction of oil (WAF; WAF-treatments). The WAF was produced using 130 liter baffled recirculating tanks (BRT) and Macondo Surrogate oil. Seawater was collected close to TABS buoy R (29˚38.1000’N, 93˚38.5020’W) in the Gulf of Mexico 16 July 2016. This water was greenish and turbid. The three Control tanks were filled directly with seawater from the storage tank (90 liters each). WAF was prepared by mixing 25 ml (5 ml ~ every 30 min for 2.5 hrs.) of Macondo Surrogate oil into 130 L of seawater then mixing for 12 to 24 hrs. The WAF was then introduced into the WAF mesocosm tanks (87 L). Macronutrients were added at F/8 to all mesocosms. Plankton (≥63 µm), which was collected at the same side as the seawater, was added. This concentrated plankton sample was introduced to the tanks and stirred (2 L to each mesocosm) immediately prior to starting the experiments. Banks of lights were placed behind each of the glass tanks and a 12:12 light dark cycle used (7pm to 7 am dark). Samples were drawn through a 5 cm diameter Teflon faucet, positioned 10 cm from the bottom of each tank. Samples were collected every 24 hrs. up to 72 hrs. TEP concentrations were determined in triplicate twice daily (every 12 hours) using the colorimetric method and are expressed in Gum Xanthan equivalents (GXeq.) (Passow and Alldredge, 1995). CSP was determined once daily (every 24 hrs.) using the colorimetric method (Cisternas-Novoa et al.).||micrometer to liters, days|Coefficient of variation (standard deviation / average) were calculated from the triplicate TEP analysis to identify problems. Averages and standard deviations were calculated from the three replicate mesocosms of each treatment.|Cisternas Novoa, C., C. Lee, A. Engel (2015). Transparent exopolymer particles (TEP) and Coomassie stainable particles (CSP): Differences between their origin and vertical distributions in the ocean. Marine Chemistry, 175: 56-71, doi.org/10.1016/j.marchem.2015.03.009. Passow, U., Alldredge, A.L., 1995. A dye-binding assay for the spectrophotometric measurement of transparent exopolymer particles (TEP). Limnology and Oceanography 40 (7), 1326-1335.
Return To Index

Distribution info: Distribution: Distributor: Distributor: Distributor contact: Responsible party: Organisation name: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC) Contact info: Contact: Phone: Telephone: Voice: 3618253604 Address: Address: Delivery point: 6300 Ocean Drive City: Corpus Christi Administrative area: TX Postal code: 78412 Country: USA Electronic mail address: griidc@gomri.org Online Resource: Online Resource: Linkage: URL: https://data.gulfresearchinitiative.org Role: Role code: distributor Distributor format: Format: Name: csv Version: inapplicable File decompression technique: Distributor transfer options: Digital transfer options: Transfer size: 0.0012 Online: Online Resource: Linkage: URL: https://data.gulfresearchinitiative.org/data/R4.x263.000:0010 Protocol: https Name: Data Landing Page Description: GRIIDC dataset landing page Function: Online function code: information
Return To Index

Metadata maintenance: Maintenance information: Maintenance and update frequency: unknown Maintenance note: This ISO metadata record was automatically generated from information provided to GRIIDC for dataset: R4.x263.000:0010 on 2020-09-24T09:01:32-05:00
Return To Index

Dataset Links: