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Mesozooplankton size-fractionated gut fluorescence data collected during R/V Point Sur cruise PTS03 from 2016-07-24 to 2016-07-30Consortium for Oil Spill Exposure Pathways in Coastal River-Dominated Ecosystems (CONCORDE)
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Suggested Citation:
Christian Briseño-Avena; Frank J. Hernandez; Robert K. Cowen; Jeffrey W. Krause (2018) Mesozooplankton size-fractionated gut fluorescence data collected during R/V Point Sur cruise PTS03 from 2016-07-24 to 2016-07-30. Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University-Corpus Christi. doi:10.7266/N7T1522T
Abstract:
This dataset contains measurements of mesozooplankton gut phaeopigments and associated metadata collected from 13 sampling locations during R/V Point Sur cruise PTS03 from 2016-07-24 to 2016-07-30 in the Mississippi Bight, Gulf of Mexico. Mesozooplankton samples were taken during the spring of 2015 using a mininess (BIONESS) tow system. Samples were taken from three nets, corresponding to three depth intervals (e.g., bottom, midwater, surface). Nets had 202-micrometer mesh size, but in some cases, samples were taken from nets with 0.333-micrometer mesh due to system issues. Samples were transferred into a bucket immediately after retrieval and carbonated water was added to the sample to stop or avoid further 'net feeding' or regurgitation from individual zooplankton. Typically, each net sample was split into two aliquots using a Folsom Splitter aboard the ship. The 1/2 aliquot separated from the original sample was then split further into two subsamples: one was used to measure mesozooplankton gut fluorescence and the other was processed for dry weight (reported separately). When samples were too thick, the initial sample was split into smaller aliquots (clearly indicated in the dataset). The corresponding aliquot was then size fractionated into five size bins using sieves with meshes measuring: 0.212, 0.5, 1, 2, and 4.75 mm. Filtered seawater was used during sample split and size fractionation. After fractionation, each sample was poured onto a GF filter (2.5 mm diameter) using a filtering system aboard the ship, rinsed with DI water, wrapped in aluminum foil and quick-frozen immediately after filtering by depositing the sample into liquid nitrogen. Before filtering, each sample was kept in the dark in an ice bath. Samples were processed following Strickland and Parsons (1968) spectrophotometric determination of phaeopigments protocol. These data will be used in combination with other mesozooplankton net data to study zooplankton processes in the river-dominated northern Gulf of Mexico. Data is reported in units of micrograms of phaeopigments per liter. Related mesozooplankton size-fractionated dry weight data and BIONESS profile data are available under GRIIDC UDIs R4.x260.000:0028 and R4.x260.000:0003 respectively. The gut fluorescence data come from the same net analyzed for dry weight, and both gut fluorescence and dry weight data come from the net tows, BIONESS profile datasets, which contains all the detailed information about the station, net start/end coordinates, and sampling information.
Purpose:
These data will be used in combination with other mesozooplankton net data to study zooplankton grazing rates in the river-dominated northern Gulf of Mexico.
Theme Keywords:
Gut fluorescence, Phaeopigments, Mesozooplankton, plankton net, BIONESS, mininess, sample depth, temperature, salinity
File Format:
xlsx, csv
Filename:
mesozooplankton_gut_fluorescence_pts03.zip (20.25 KB)
Dataset Downloads:
3
Mesozooplankton size-fractionated gut fluorescence data collected during R/V Point Sur cruise PTS03 from 2016-07-24 to 2016-07-30
Identification Information
Distribution Information
Metadata Maintenance Information
Metadata:
File identifier:
R4.x260.000-0036-metadata.xml
Language:
eng; USA
Character set:
Character set code:
utf8
Hierarchy level:
Scope code:
dataset
Metadata author:
Responsible party:
Individual name:
Christian Briseno-Avena
Organisation name:
Oregon State University / Hatfield Marine Science Center
Position name:
Postdoctoral Researcher
Contact info:
Contact:
Phone:
Telephone:
Voice:
5418670314
Facsimile:
Address:
Address:
Delivery point:
2030 SE Marine Science Dr
City:
Newport
Administrative area:
Oregon
Postal code:
97365
Country:
USA
Electronic mail address:
brisenoc@oregonstate.edu
Role:
Role code:
pointOfContact
Date stamp:
2018-09-27T21:48:56+00:00
Metadata standard name:
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
Metadata standard version:
ISO 19115-2:2009(E)
Dataset URI:
https://data.gulfresearchinitiative.org/metadata/R4.x260.000:0036
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Identification info:
Data identification:
Citation:
Citation:
Title:
Mesozooplankton size-fractionated gut fluorescence data collected during R/V Point Sur cruise PTS03 from
2016-07-24 to 2016-07-30
Alternate title:
Date:
Date:
Date:
2018-09-04
Date type:
Date type code:
publication
Abstract:
This dataset contains measurements of mesozooplankton gut phaeopigments and associated metadata collected from
13 sampling locations during R/V Point Sur cruise PTS03 from 2016-07-24 to 2016-07-30 in the Mississippi
Bight, Gulf of Mexico. Mesozooplankton samples were taken during the spring of 2015 using a mininess
(BIONESS) tow system. Samples were taken from three nets, corresponding to three depth intervals (e.g.,
bottom, midwater, surface). Nets had 202-micrometer mesh size, but in some cases, samples were taken from
nets with 0.333-micrometer mesh due to system issues. Samples were transferred into a bucket immediately
after retrieval and carbonated water was added to the sample to stop or avoid further 'net feeding' or
regurgitation from individual zooplankton. Typically, each net sample was split into two aliquots using a
Folsom Splitter aboard the ship. The 1/2 aliquot separated from the original sample was then split further
into two subsamples: one was used to measure mesozooplankton gut fluorescence and the other was processed
for dry weight (reported separately). When samples were too thick, the initial sample was split into smaller
aliquots (clearly indicated in the dataset). The corresponding aliquot was then size fractionated into five
size bins using sieves with meshes measuring: 0.212, 0.5, 1, 2, and 4.75 mm. Filtered seawater was used
during sample split and size fractionation. After fractionation, each sample was poured onto a GF filter
(2.5 mm diameter) using a filtering system aboard the ship, rinsed with DI water, wrapped in aluminum foil
and quick-frozen immediately after filtering by depositing the sample into liquid nitrogen. Before
filtering, each sample was kept in the dark in an ice bath. Samples were processed following Strickland and
Parsons (1968) spectrophotometric determination of phaeopigments protocol. These data will be used in
combination with other mesozooplankton net data to study zooplankton processes in the river-dominated
northern Gulf of Mexico. Data is reported in units of micrograms of phaeopigments per liter. Related
mesozooplankton size-fractionated dry weight data and BIONESS profile data are available under GRIIDC UDIs
R4.x260.000:0028 and R4.x260.000:0003 respectively. The gut fluorescence data come from the same net
analyzed for dry weight, and both gut fluorescence and dry weight data come from the net tows, BIONESS
profile datasets, which contains all the detailed information about the station, net start/end coordinates,
and sampling information.
Purpose:
These data will be used in combination with other mesozooplankton net data to study zooplankton grazing rates
in the river-dominated northern Gulf of Mexico.
Status:
Progress code:
completed
Point of contact:
Responsible party:
Individual name:
Christian Briseno-Avena
Organisation name:
Oregon State University / Hatfield Marine Science Center
Position name:
Postdoctoral Researcher
Contact info:
Contact:
Phone:
Telephone:
Voice:
5418670314
Facsimile:
Address:
Address:
Delivery point:
2030 SE Marine Science Dr
City:
Newport
Administrative area:
Oregon
Postal code:
97365
Country:
USA
Electronic mail address:
brisenoc@oregonstate.edu
Role:
Role code:
pointOfContact
Point of contact:
Responsible party:
Individual name:
Frank Hernandez
Organisation name:
University of Southern Mississippi / Department of Coastal Sciences
Position name:
Assistant Professor
Contact info:
Contact:
Phone:
Telephone:
Voice:
2288188800
Facsimile:
Address:
Address:
Delivery point:
Gulf Coast Research Laboratory
703 East Beach Drive
City:
Ocean Springs
Administrative area:
Mississippi
Postal code:
39564
Country:
USA
Electronic mail address:
frank.hernandez@usm.edu
Role:
Role code:
principalInvestigator
Point of contact:
Responsible party:
Individual name:
Robert (Bob) Cowen
Organisation name:
Oregon State University / Hatfield Marine Science Center
Position name:
Director
Contact info:
Contact:
Phone:
Telephone:
Voice:
5418670211
Facsimile:
Address:
Address:
Delivery point:
2030 SE Marine Science Dr
City:
Newport
Administrative area:
Oregon
Postal code:
97365
Country:
USA
Electronic mail address:
robert.cowen@oregonstate.edu
Role:
Role code:
principalInvestigator
Descriptive keywords:
Keywords:
Keyword:
Gut fluorescence
Keyword:
Phaeopigments
Keyword:
Mesozooplankton
Keyword:
plankton net
Keyword:
BIONESS
Keyword:
mininess
Keyword:
sample depth
Keyword:
temperature
Keyword:
salinity
Type:
Keyword type code:
theme
Descriptive keywords:
Keywords:
Keyword:
Mississippi Bight
Keyword:
northern Gulf of Mexico
Type:
Keyword type code:
place
Language:
eng; USA
Topic category:
Topic category code:
oceans
Topic category:
Topic category code:
biota
Extent:
Extent:
Geographic element:
Geographic bounding box:
West bound longitude:
-88.61
East bound longitude:
-87.516
South bound latitude:
29.592
North bound latitude:
30.184
Geographic element:
BoundingPolygon:
Polygon:
gml:MultiPoint:
gml:pointMember:
Point:
gml:pos:
29.592 -88.609
gml:pointMember:
Point:
gml:pos:
29.688 -88.125
gml:pointMember:
Point:
gml:pos:
29.762 -87.516
gml:pointMember:
Point:
gml:pos:
29.818 -88.61
gml:pointMember:
Point:
gml:pos:
29.823 -88.606
gml:pointMember:
Point:
gml:pos:
29.824 -88.482
gml:pointMember:
Point:
gml:pos:
29.826 -88.606
gml:pointMember:
Point:
gml:pos:
29.829 -88.61
gml:pointMember:
Point:
gml:pos:
29.84 -88.479
gml:pointMember:
Point:
gml:pos:
29.858 -88.61
gml:pointMember:
Point:
gml:pos:
30.113 -88.596
gml:pointMember:
Point:
gml:pos:
30.124 -88.129
gml:pointMember:
Point:
gml:pos:
30.184 -87.532
Temporal element:
Temporal extent:
Extent:
Time period:
Description:
ground condition
Begin date:
2016-07-24
End date:
2016-07-30
Supplemental Information:
Cruise id, station (Corridor where net was towed. MCORR= middle corridor, ECORR= eastern corridor, WCORR=
western corridor, ARRAY= mixing mooring array), Date_UTC (dd/mmm/yy, UTC), Time_UTC (time when net was
deployed, HH:MM, UTC), Latitude_Decimal (location of net deployment, decimal degrees North),
Longitude_Decimal (location of net deployment, decimal degrees West), Tow_Number (unique number ID of the
net tow deployment), Net_Number (number of the BIONESS net where the sample was obtained), Net_Mesh_um (mesh
size, micrometers), Depth_Open_m (depth where the net started sampling, m), Depth_Close_m (depth where the
net stopped sampling, m), Swept_Volume_m3 (amount of water sampled by the net, m^3),
Mean_Temperature_Celsius (mean temperature measured by the environmental sensor system during the interval
the net was collecting samples, degrees C), Mean_Salinity_PSU (mean salinity measured by the environmental
sensor system during the interval the net was collecting samples, PSU), GF_Number (unique number of the
aliquot obtained from the net), Sieve_Size_mm (size of the sieve's mesh in millimeters where the subsample
was obtained for this analysis), Net_Split_Factor (fraction of the original sample taken from the net),
Filter_Section_Factor (fraction of the GF filter used for pigment extraction and laboratory analysis),
Gut_Phaeopigments_ug_per_L (micrograms of mesozooplankton gut phaeopigments per liter), Notes (notes of
dominant organisms found in filter; notes of non-grazing organisms removed prior to pigment extraction)|GF
filters were transferred from liquid nitrogen to -80 freezers and kept there until laboratory analyses. Gut
pigments were measured using the Strickland and Parsons (1968) spectrophotometric determination of
phaeopigments protocol. GF filters were processed in the following manner: for subsamples coming from sieve
sizes 4.75 & 2 mm the whole filter was used for pigment extraction; for subsamples from sieve size 1 mm, 1/4
of the filter was processed; and 1/8 of the filter was used for filters with samples from sieve sizes 0.5
and 0.212 mm. Pigment extraction was done in 90% acetone as per standard pigment analyses protocols and
using the acidification method (HCl). All laboratory analyses were conducted in dark conditions as much as
possible to avoid pigment photodegradation. Pigment measurements were carried out on an SpectraMax M2
(Molecular Devices) spectrophotometer using a cuvette of 1 cm path length.
The nets were fished for about 6 minutes each. Volume swept and depth bin intervals were targeted to three
depth bins: deep, mid-water column, and near the surface. For these data, samples from 3 out of the 9 nets
used on the BIONESS dataset were used. The start and end coordinates and time for each of these 3 nets are
very close to each other that end towing information are not provided in this dataset. However, the full
station metadata, net data, and detailed environmental data for each net can be found in the BIONESS dataset
under GRIIDC UDI R4.x260.000:0003.||||Briseño-Avena, Christian, Alison L. Deary, Robert K. Cowen, Frank J.
Hernandez, and Jeffrey W. Krause. 2018. Mesozooplankton Size-Fractionated Dry Weight, cruise PTS03, July
2016 . Consortium for Oil Spill Exposure Pathways in Coastal River-Dominated Ecosystems (CONCORDE). DOI:
10.7266/N7QJ7FN0
Frank Hernandez, Carla Culpepper. 2018. BIONESS profile and plankton sample collection data, cruise PTS03,
northern Gulf of Mexico, July 2016 . Consortium for Oil Spill Exposure Pathways in Coastal River-Dominated
Ecosystems (CONCORDE). DOI: 10.7266/N74T6GQD
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Distribution info:
Distribution:
Distributor:
Distributor:
Distributor contact:
Responsible party:
Organisation name:
Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC)
Contact info:
Contact:
Phone:
Telephone:
Voice:
3618253604
Address:
Address:
Delivery point:
6300 Ocean Drive
City:
Corpus Christi
Administrative area:
TX
Postal code:
78412
Country:
USA
Electronic mail address:
griidc@gomri.org
Online Resource:
Online Resource:
Linkage:
URL:
https://data.gulfresearchinitiative.org
Role:
Role code:
distributor
Distributor format:
Format:
Name:
xlsx, csv
Version:
inapplicable
File decompression technique:
zip
Distributor transfer options:
Digital transfer options:
Transfer size:
0.0203
Online:
Online Resource:
Linkage:
URL:
https://data.gulfresearchinitiative.org/data/R4.x260.000:0036
Protocol:
https
Name:
Data Landing Page
Description:
GRIIDC dataset landing page
Function:
Online function code:
information
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Metadata maintenance:
Maintenance information:
Maintenance and update frequency:
unknown
Maintenance note:
This ISO metadata record was automatically generated from information provided to GRIIDC for dataset:
R4.x260.000:0036 on 2020-01-21T19:30:07-06:00
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