Mesozooplankton size-fractionated gut fluorescence data collected during R/V Point Sur cruise PTS02 from 2016-03-30 to 2016-04-09

Consortium for Oil Spill Exposure Pathways in Coastal River-Dominated Ecosystems (CONCORDE)

DOI:
10.7266/N7XS5SSF
 
UDI:
R4.x260.000:0035
Last Update:
Sep 11 2018 19:11 UTC
 
Dataset Author(s):
Christian Briseño-Avena; Frank J. Hernandez; Robert K. Cowen; Jeffrey W. Krause
Point of Contact:
Briseno-Avena, Christian
Oregon State University / Hatfield Marine Science Center
2030 SE Marine Science Dr
Newport, Oregon  97365
USA
brisenoc@oregonstate.edu
Funding Source:
RFP-IV
Data Collection Period:
2016-03-30 to 2016-04-09

Identified Submitted In-Review Available
3 3 3 3

Suggested Citation:

Christian Briseño-Avena; Frank J. Hernandez; Robert K. Cowen; Jeffrey W. Krause (2018) Mesozooplankton size-fractionated gut fluorescence data collected during R/V Point Sur cruise PTS02 from 2016-03-30 to 2016-04-09. Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University-Corpus Christi. doi:10.7266/N7XS5SSF

Abstract:

This dataset contains measurements of mesozooplankton gut phaeopigments and associated metadata collected from 20 sampling locations during R/V Point Sur cruise PTS02 from 2016-03-30 to 2016-04-09 in the Mississippi Bight, Gulf of Mexico. Mesozooplankton samples were taken during the spring of 2015 using a mininess (BIONESS) tow system. Samples were taken from three nets, corresponding to three depth intervals (e.g., bottom, midwater, surface). Nets had 202-micrometer mesh size, but in some cases, samples were taken from nets with 0.333-micrometer mesh due to system issues. Samples were transferred into a bucket immediately after retrieval and carbonated water was added to the sample to stop or avoid further 'net feeding' or regurgitation from individual zooplankton. Typically, each net sample was split into two aliquots using a Folsom Splitter aboard the ship. The 1/2 aliquot separated from the original sample was then split further into two subsamples: one was used to measure mesozooplankton gut fluorescence and the other was processed for dry weight (reported separately). When samples were too thick, the initial sample was split into smaller aliquots (clearly indicated in the dataset). The corresponding aliquot was then size fractionated into five size bins using sieves with meshes measuring: 0.212, 0.5, 1, 2, and 4.75 mm. Filtered seawater was used during sample split and size fractionation. After fractionation, each sample was poured onto a GF filter (2.5 mm diameter) using a filtering system aboard the ship, rinsed with DI water, wrapped in aluminum foil and quick-frozen immediately after filtering by depositing the sample into liquid nitrogen. Before filtering, each sample was kept in the dark in an ice bath. Samples were processed following Strickland and Parsons (1968) spectrophotometric determination of phaeopigments protocol. These data will be used in combination with other mesozooplankton net data to study zooplankton processes in the river-dominated northern Gulf of Mexico. Data is reported in units of micrograms of phaeopigments per liter. Related mesozooplankton size-fractionated dry weight data and BIONESS profile data are available under GRIIDC UDIs R4.x260.000:0027 and R4.x260.000:0002 respectively. The gut fluorescence data come from the same net analyzed for dry weight, and both gut fluorescence and dry weight data come from the net tows, BIONESS profile datasets, which contains all the detailed information about the station, net start/end coordinates, and sampling information.

Purpose:

These data will be used in combination with other mesozooplankton net data to study zooplankton grazing rates in the river-dominated northern Gulf of Mexico.

Theme Keywords:

Gut fluorescence, Phaeopigments, Mesozooplankton, plankton net, BIONESS, mininess, sample depth, temperature, salinity

File Format:

xlsx, csv

Filename:

mesozooplankton_gut_fluorescence_pts02.zip (22.45 KB)

Dataset Downloads:

3

Mesozooplankton size-fractionated gut fluorescence data collected during R/V Point Sur cruise PTS02 from 2016-03-30 to 2016-04-09



Identification Information
Distribution Information
Metadata Maintenance Information

Metadata: 
  File identifier: 
      R4.x260.000-0035-metadata.xml
  Language: 
      eng; USA
  Character set: 
    Character set code: 
      utf8
  Hierarchy level: 
    Scope code: 
      dataset
  Metadata author: 
    Responsible party: 
      Individual name: 
          Christian Briseno-Avena
      Organisation name: 
          Oregon State University / Hatfield Marine Science Center
      Position name: 
          Postdoctoral Researcher
      Contact info: 
        Contact: 
          Phone: 
            Telephone: 
              Voice: 
                  5418670314
              Facsimile: 
          Address: 
            Address: 
              Delivery point: 
                  2030 SE Marine Science Dr
              City: 
                  Newport
              Administrative area: 
                  Oregon
              Postal code: 
                  97365
              Country: 
                  USA
              Electronic mail address: 
                  brisenoc@oregonstate.edu
      Role: 
        Role code: 
          pointOfContact
  Date stamp: 
      2018-09-27T20:05:58+00:00
  Metadata standard name: 
      ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
  Metadata standard version: 
      ISO 19115-2:2009(E)
  Dataset URI: 
      https://data.gulfresearchinitiative.org/metadata/R4.x260.000:0035
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Identification info: Data identification: Citation: Citation: Title: Mesozooplankton size-fractionated gut fluorescence data collected during R/V Point Sur cruise PTS02 from 2016-03-30 to 2016-04-09 Alternate title: Date: Date: Date: 2018-09-04 Date type: Date type code: publication Abstract: This dataset contains measurements of mesozooplankton gut phaeopigments and associated metadata collected from 20 sampling locations during R/V Point Sur cruise PTS02 from 2016-03-30 to 2016-04-09 in the Mississippi Bight, Gulf of Mexico. Mesozooplankton samples were taken during the spring of 2015 using a mininess (BIONESS) tow system. Samples were taken from three nets, corresponding to three depth intervals (e.g., bottom, midwater, surface). Nets had 202-micrometer mesh size, but in some cases, samples were taken from nets with 0.333-micrometer mesh due to system issues. Samples were transferred into a bucket immediately after retrieval and carbonated water was added to the sample to stop or avoid further 'net feeding' or regurgitation from individual zooplankton. Typically, each net sample was split into two aliquots using a Folsom Splitter aboard the ship. The 1/2 aliquot separated from the original sample was then split further into two subsamples: one was used to measure mesozooplankton gut fluorescence and the other was processed for dry weight (reported separately). When samples were too thick, the initial sample was split into smaller aliquots (clearly indicated in the dataset). The corresponding aliquot was then size fractionated into five size bins using sieves with meshes measuring: 0.212, 0.5, 1, 2, and 4.75 mm. Filtered seawater was used during sample split and size fractionation. After fractionation, each sample was poured onto a GF filter (2.5 mm diameter) using a filtering system aboard the ship, rinsed with DI water, wrapped in aluminum foil and quick-frozen immediately after filtering by depositing the sample into liquid nitrogen. Before filtering, each sample was kept in the dark in an ice bath. Samples were processed following Strickland and Parsons (1968) spectrophotometric determination of phaeopigments protocol. These data will be used in combination with other mesozooplankton net data to study zooplankton processes in the river-dominated northern Gulf of Mexico. Data is reported in units of micrograms of phaeopigments per liter. Related mesozooplankton size-fractionated dry weight data and BIONESS profile data are available under GRIIDC UDIs R4.x260.000:0027 and R4.x260.000:0002 respectively. The gut fluorescence data come from the same net analyzed for dry weight, and both gut fluorescence and dry weight data come from the net tows, BIONESS profile datasets, which contains all the detailed information about the station, net start/end coordinates, and sampling information. Purpose: These data will be used in combination with other mesozooplankton net data to study zooplankton grazing rates in the river-dominated northern Gulf of Mexico. Status: Progress code: completed Point of contact: Responsible party: Individual name: Christian Briseno-Avena Organisation name: Oregon State University / Hatfield Marine Science Center Position name: Postdoctoral Researcher Contact info: Contact: Phone: Telephone: Voice: 5418670314 Facsimile: Address: Address: Delivery point: 2030 SE Marine Science Dr City: Newport Administrative area: Oregon Postal code: 97365 Country: USA Electronic mail address: brisenoc@oregonstate.edu Role: Role code: pointOfContact Point of contact: Responsible party: Individual name: Robert (Bob) Cowen Organisation name: Oregon State University / Hatfield Marine Science Center Position name: Director Contact info: Contact: Phone: Telephone: Voice: 5418670211 Facsimile: Address: Address: Delivery point: 2030 SE Marine Science Dr City: Newport Administrative area: Oregon Postal code: 97365 Country: USA Electronic mail address: robert.cowen@oregonstate.edu Role: Role code: principalInvestigator Point of contact: Responsible party: Individual name: Frank Hernandez Organisation name: University of Southern Mississippi / Department of Coastal Sciences Position name: Assistant Professor Contact info: Contact: Phone: Telephone: Voice: 2288188800 Facsimile: Address: Address: Delivery point: Gulf Coast Research Laboratory 703 East Beach Drive City: Ocean Springs Administrative area: Mississippi Postal code: 39564 Country: USA Electronic mail address: frank.hernandez@usm.edu Role: Role code: principalInvestigator Descriptive keywords: Keywords: Keyword: Gut fluorescence Keyword: Phaeopigments Keyword: Mesozooplankton Keyword: plankton net Keyword: BIONESS Keyword: mininess Keyword: sample depth Keyword: temperature Keyword: salinity Type: Keyword type code: theme Descriptive keywords: Keywords: Keyword: Mississippi Bight Keyword: northern Gulf of Mexico Type: Keyword type code: place Language: eng; USA Topic category: Topic category code: oceans Topic category: Topic category code: biota Extent: Extent: Geographic element: Geographic bounding box: West bound longitude: -88.612 East bound longitude: -87.528 South bound latitude: 29.625 North bound latitude: 30.198 Geographic element: BoundingPolygon: Polygon: gml:MultiPoint: gml:pointMember: Point: gml:pos: 29.625 -88.612 gml:pointMember: Point: gml:pos: 29.662 -88.126 gml:pointMember: Point: gml:pos: 29.673 -88.126 gml:pointMember: Point: gml:pos: 29.681 -88.125 gml:pointMember: Point: gml:pos: 29.683 -88.605 gml:pointMember: Point: gml:pos: 29.692 -88.095 gml:pointMember: Point: gml:pos: 29.694 -88.069 gml:pointMember: Point: gml:pos: 29.716 -87.528 gml:pointMember: Point: gml:pos: 29.781 -88.09 gml:pointMember: Point: gml:pos: 30.093 -88.608 gml:pointMember: Point: gml:pos: 30.104 -88.004 gml:pointMember: Point: gml:pos: 30.109 -88.092 gml:pointMember: Point: gml:pos: 30.119 -88.549 gml:pointMember: Point: gml:pos: 30.121 -88.125 gml:pointMember: Point: gml:pos: 30.127 -88.108 gml:pointMember: Point: gml:pos: 30.166 -88.01 gml:pointMember: Point: gml:pos: 30.192 -87.529 gml:pointMember: Point: gml:pos: 30.194 -87.531 gml:pointMember: Point: gml:pos: 30.197 -88.159 gml:pointMember: Point: gml:pos: 30.198 -88.207 Temporal element: Temporal extent: Extent: Time period: Description: ground condition Begin date: 2016-03-30 End date: 2016-04-09 Supplemental Information: Cruise id, station (Corridor where net was towed. MCORR= middle corridor, ECORR= eastern corridor, WCORR= western corridor, ARRAY= mixing mooring array), Date_UTC (dd/mmm/yy, UTC), Time_UTC (time when net was deployed, HH:MM, UTC), Latitude_Decimal (location of net deployment, decimal degrees North), Longitude_Decimal (location of net deployment, decimal degrees West), Tow_Number (unique number ID of the net tow deployment), Net_Number (number of the BIONESS net where the sample was obtained), Net_Mesh_um (mesh size, micrometers), Depth_Open_m (depth where the net started sampling, m), Depth_Close_m (depth where the net stopped sampling, m), Swept_Volume_m3 (amount of water sampled by the net, m^3), Mean_Temperature_Celsius (mean temperature measured by the environmental sensor system during the interval the net was collecting samples, degrees C), Mean_Salinity_PSU (mean salinity measured by the environmental sensor system during the interval the net was collecting samples, PSU), GF_Number (unique number of the aliquot obtained from the net), Sieve_Size_mm (size of the sieve's mesh in millimeters where the subsample was obtained for this analysis), Net_Split_Factor (fraction of the original sample taken from the net), Filter_Section_Factor (fraction of the GF filter used for pigment extraction and laboratory analysis), Gut_Phaeopigments_ug_per_L (micrograms of mesozooplankton gut phaeopigments per liter), Notes (notes of dominant organisms found in filter; notes of non-grazing organisms removed prior to pigment extraction).|GF filters were transferred from liquid nitrogen to -80 freezers and kept there until laboratory analyses. Gut pigments were measured using the Strickland and Parsons (1968) spectrophotometric determination of phaeopigments protocol. GF filters were processed in the following manner: for subsamples coming from sieve sizes 4.75 & 2 mm the whole filter was used for pigment extraction; for subsamples from sieve size 1 mm, 1/4 of the filter was processed; and 1/8 of the filter was used for filters with samples from sieve sizes 0.5 and 0.212 mm. Pigment extraction was done in 90% acetone as per standard pigment analyses protocols and using the acidification method (HCl). All laboratory analyses were conducted in dark conditions as much as possible to avoid pigment photodegradation. Pigment measurements were carried out on an SpectraMax M2 (Molecular Devices) spectrophotometer using a cuvette of 1 cm path length.
          
The nets were fished for about 6 minutes each. Volume swept and depth bin intervals were targeted to three depth bins: deep, mid-water column, and near the surface. For these data, samples from 3 out of the 9 nets used on the BIONESS dataset were used. The start and end coordinates and time for each of these 3 nets are very close to each other that end towing information are not provided in this dataset. However, the full station metadata, net data, and detailed environmental data for each net can be found in the BIONESS dataset under GRIIDC UDI R4.x260.000:0002.||||Briseño-Avena, Christian, Alison L. Deary, Robert K. Cowen, Frank J. Hernandez, and Jeffrey W. Krause. 2018. Mesozooplankton Size-Fractionated Dry Weight, cruise PTS02, Spring 2016 . Consortium for Oil Spill Exposure Pathways in Coastal River-Dominated Ecosystems (CONCORDE). DOI: 10.7266/N75H7DB7
          
Hernandez, Frank, Culpepper, Carla, Deary, Alison. 2018. BIONESS profile and plankton sample collection data, cruise PTS02, northern Gulf of Mexico, March–April 2016 . Consortium for Oil Spill Exposure Pathways in Coastal River-Dominated Ecosystems (CONCORDE). DOI: 10.7266/N7X928PX
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Distribution info: Distribution: Distributor: Distributor: Distributor contact: Responsible party: Organisation name: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC) Contact info: Contact: Phone: Telephone: Voice: 3618253604 Address: Address: Delivery point: 6300 Ocean Drive City: Corpus Christi Administrative area: TX Postal code: 78412 Country: USA Electronic mail address: griidc@gomri.org Online Resource: Online Resource: Linkage: URL: https://data.gulfresearchinitiative.org Role: Role code: distributor Distributor format: Format: Name: xlsx, csv Version: inapplicable File decompression technique: zip Distributor transfer options: Digital transfer options: Transfer size: 0.0224 Online: Online Resource: Linkage: URL: https://data.gulfresearchinitiative.org/data/R4.x260.000:0035 Protocol: https Name: Data Landing Page Description: GRIIDC dataset landing page Function: Online function code: information
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Metadata maintenance: Maintenance information: Maintenance and update frequency: unknown Maintenance note: This ISO metadata record was automatically generated from information provided to GRIIDC for dataset: R4.x260.000:0035 on 2019-10-18T23:23:41-05:00
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