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Mesozooplankton Size-Fractionated Dry Weight, cruise PTS02, Spring 2016

Consortium for Oil Spill Exposure Pathways in Coastal River-Dominated Ecosystems (CONCORDE)

DOI:
10.7266/N75H7DB7
 
UDI:
R4.x260.000:0027
Last Update:
Jul 12 2017 20:39 UTC
 
Dataset Author(s):
Briseño-Avena, Christian, Alison L. Deary, Robert K. Cowen, Frank J. Hernandez, and Jeffrey W. Krause
Point of Contact:
Briseno-Avena, Christian
Oregon State University / Hatfield Marine Science Center
2030 SE Marine Science Dr
Newport, Oregon  97365
USA
brisenoc@oregonstate.edu
Funding Source:
RFP-IV
Data Collection Period:
2016-03-30 to 2016-04-09

Identified Submitted Review Available
3 3 3 3

Abstract:

Mesozooplankton samples were taken during the spring of 2016 between March 30 and April 9 aboard R/V Point Sur using a mininess (BIONESS) tow system. Samples were taken from three nets, corresponding to three depth intervals (e.g. bottom, midwater, surface). Nets had 202 micrometer mesh size, but in some cases samples were taken from nets with 0.333 micrometer mesh. Samples were transferred into a bucket immediately after retrieval and carbonated water was added to the sample to stop or avoid further 'net feeding' or regurgitation from individual zooplankton. Typically, each net sample was split in two aliquots using a Folsom Splitter aboard the ship. The 1/2 aliquot separated from the original sample was then split further in two subsamples: one was used to measure mesozooplankton dry weight and the other was processed for gut fluorescence (data reported in GRIIDC UDI: R4.x260.000:0035). When samples were too thick, the initial sample was split into smaller aliquots (clearly indicated in the dataset). The corresponding aliquot was then size fractionated into five classes using sieves with meshes measuring: 0.212, 0.5, 1, 2, and 4.75 mm. Filtered seawater was used during sample split and size fractionation. Next, each size fraction was filtered onto pre-weighted nitex circles (2.5 mm diameter) with a 0.202 mesh size. Nitex circles were individually put in labeled plastic petri dishes and stored stacked inside sealed plastic bags to avoid moisture from getting in touch with the nitex before using them. After fractionation, each sample was poured onto a nitex circle using a filtering system aboard the ship, rinsed with DI water, transferred to its pre-labeled petri dish and frozen immediately after filtering. At the lab, the samples were dried up in an oven at 60 degrees Celsius over a period of 48 hours and weighted immediately after taking each sample out of the oven. The difference between the weight of the filter minus the weight after drying the sample constituted the mesozooplankton dry weight. These data will be used in combination with other mesozooplankton net data to study zooplankton processes in the river-dominated northern Gulf of Mexico.

Purpose:

The dataset was developed to provide mesozooplankton size-fractionated information that will be used, among others, to normalize mesozooplankton grazing rates measured through the gut fluorescence.

Theme Keywords:

Mesozooplankton, Dry Weight, Biomass, Size Fractionated, Mississippi Bight, Continental Shelf, Mobile River Delta

File Format:

csv

Dataset Downloads:

1

Mesozooplankton Size-Fractionated Dry Weight, cruise PTS02, Spring 2016



Identification Information
Distribution Information
Metadata Maintenance Information

Metadata: 
  File identifier: 
      R4.x260.000-0027-metadata.xml
  Language: 
      eng; USA
  Character set: 
    Character set code: 
      utf8
  Hierarchy level: 
    Scope code: 
      dataset
  Metadata author: 
    Responsible party: 
      Individual name: 
          Robert (Bob) Cowen
      Organisation name: 
          Oregon State University / Hatfield Marine Science Center
      Position name: 
          Director
      Contact info: 
        Contact: 
          Phone: 
            Telephone: 
              Voice: 
                  5418670211
              Facsimile: 
          Address: 
            Address: 
              Delivery point: 
                  2030 SE Marine Science Dr
              City: 
                  Newport
              Administrative area: 
                  Oregon
              Postal code: 
                  97365
              Country: 
                  USA
              Electronic mail address: 
                  robert.cowen@oregonstate.edu
      Role: 
        Role code: 
          pointOfContact
  Date stamp: 
      2018-08-11T13:36:37+00:00
  Metadata standard name: 
      ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
  Metadata standard version: 
      ISO 19115-2:2009(E)
  Dataset URI: 
      https://data.gulfresearchinitiative.org/metadata/R4.x260.000:0027
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Identification info: Data identification: Citation: Citation: Title: Mesozooplankton Size-Fractionated Dry Weight, cruise PTS02, Spring 2016 Alternate title: ISIIS Raw Physical Data Date: Date: Date: 2016-10-19 Date type: Date type code: creation Abstract: Mesozooplankton samples were taken during the spring of 2016 between March 30 and April 9 aboard R/V Point Sur using a mininess (BIONESS) tow system. Samples were taken from three nets, corresponding to three depth intervals (e.g. bottom, midwater, surface). Nets had 202 micrometer mesh size, but in some cases samples were taken from nets with 0.333 micrometer mesh. Samples were transferred into a bucket immediately after retrieval and carbonated water was added to the sample to stop or avoid further 'net feeding' or regurgitation from individual zooplankton. Typically, each net sample was split in two aliquots using a Folsom Splitter aboard the ship. The 1/2 aliquot separated from the original sample was then split further in two subsamples: one was used to measure mesozooplankton dry weight and the other was processed for gut fluorescence (data reported in GRIIDC UDI: R4.x260.000:0035). When samples were too thick, the initial sample was split into smaller aliquots (clearly indicated in the dataset). The corresponding aliquot was then size fractionated into five classes using sieves with meshes measuring: 0.212, 0.5, 1, 2, and 4.75 mm. Filtered seawater was used during sample split and size fractionation. Next, each size fraction was filtered onto pre-weighted nitex circles (2.5 mm diameter) with a 0.202 mesh size. Nitex circles were individually put in labeled plastic petri dishes and stored stacked inside sealed plastic bags to avoid moisture from getting in touch with the nitex before using them. After fractionation, each sample was poured onto a nitex circle using a filtering system aboard the ship, rinsed with DI water, transferred to its pre-labeled petri dish and frozen immediately after filtering. At the lab, the samples were dried up in an oven at 60 degrees Celsius over a period of 48 hours and weighted immediately after taking each sample out of the oven. The difference between the weight of the filter minus the weight after drying the sample constituted the mesozooplankton dry weight. These data will be used in combination with other mesozooplankton net data to study zooplankton processes in the river-dominated northern Gulf of Mexico. Purpose: The dataset was developed to provide mesozooplankton size-fractionated information that will be used, among others, to normalize mesozooplankton grazing rates measured through the gut fluorescence. Status: Progress code: completed Point of contact: Responsible party: Individual name: Christian Briseno-Avena Organisation name: Oregon State University / Hatfield Marine Science Center Position name: Postdoctoral Researcher Contact info: Contact: Phone: Telephone: Voice: 5418670314 Facsimile: Address: Address: Delivery point: 2030 SE Marine Science Dr City: Newport Administrative area: Oregon Postal code: 97365 Country: USA Electronic mail address: brisenoc@oregonstate.edu Role: Role code: pointOfContact Point of contact: Responsible party: Individual name: Robert (Bob) Cowen Organisation name: Oregon State University / Hatfield Marine Science Center Position name: Director Contact info: Contact: Phone: Telephone: Voice: 5418670211 Facsimile: Address: Address: Delivery point: 2030 SE Marine Science Dr City: Newport Administrative area: Oregon Postal code: 97365 Country: USA Electronic mail address: robert.cowen@oregonstate.edu Role: Role code: pointOfContact Descriptive keywords: Keywords: Keyword: Mesozooplankton Keyword: Dry Weight Keyword: Biomass Keyword: Size Fractionated Keyword: Mississippi Bight Keyword: Continental Shelf Keyword: Mobile River Delta Type: Keyword type code: theme Descriptive keywords: Keywords: Keyword: Gulf of Mexico Type: Keyword type code: place Language: eng; USA Topic category: Topic category code: biota Topic category: Topic category code: oceans Extent: Extent: Geographic element: Geographic bounding box: West bound longitude: -88.612 East bound longitude: -87.0097 South bound latitude: 29.6252 North bound latitude: 30.1978 Geographic element: BoundingPolygon: Polygon: gml:MultiPoint: gml:pointMember: Point: gml:pos: 30.1938 -87.5306 gml:pointMember: Point: gml:pos: 30.1376 -88.1228 gml:pointMember: Point: gml:pos: 29.6812 -88.1246 gml:pointMember: Point: gml:pos: 30.0913 -88.6082 gml:pointMember: Point: gml:pos: 29.6252 -88.612 gml:pointMember: Point: gml:pos: 30.1205 -88.1253 gml:pointMember: Point: gml:pos: 29.6623 -88.1262 gml:pointMember: Point: gml:pos: 30.1921 -87.5294 gml:pointMember: Point: gml:pos: 29.7157 -87.5284 gml:pointMember: Point: gml:pos: 30.1042 -88.092 gml:pointMember: Point: gml:pos: 29.6733 -88.1264 gml:pointMember: Point: gml:pos: 30.1194 -88.5318 gml:pointMember: Point: gml:pos: 29.6834 -88.6055 gml:pointMember: Point: gml:pos: 29.7808 -88.0899 gml:pointMember: Point: gml:pos: 29.6942 -88.0964 gml:pointMember: Point: gml:pos: 29.6921 -88.095 gml:pointMember: Point: gml:pos: 30.1039 -88.0044 gml:pointMember: Point: gml:pos: 30.1274 -88.1084 gml:pointMember: Point: gml:pos: 30.1978 -88.2067 gml:pointMember: Point: gml:pos: 30.1969 -88.159 gml:pointMember: Point: gml:pos: 30.1661 -87.0097 Temporal element: Temporal extent: Extent: Time period: Description: ground condition Begin date: 2016-03-30 End date: 2016-04-09 Supplemental Information: CRUISE_ID = research cruise unique identifier. YEAR = Year sample was collected. Month = Month sample was collected. Day = Day sample was collected. Time_UTC_HH = hour sample was collected, in UTC. Time_UTC_MM = minutes when sample was collected, in UTC. Latitude = Latitude (decimal). Longitude = Longitude (decimal). Negative sign indicate latitude west. Sampling_Type = Name assigned to the transect or location in which the station took place, usually indicating the type of study. Tow_ID = Unique Net Tow indentifier. MN indicates it was mininess net. Net_ID = mininess number from which the sample was taken. Petri_Dish_Number = label on the petri dish for each pre-weighted nitex circle. Mesh_Size_um = mesh size of the net used during tow. Gear_Type = The gear used for the plankton tow. Size_Fraction_mm = size of the sieve mesh, the values correspond to, in millimeters. Pre_Bake_Weight_g = weight of the nitex circle alone prior to utilization (i.e. blank weight). Post_Bake_Weight_g = weight of the nitex circle and mesozooplankton sample combined. Balance_Offset_g = two different balances were used in some cases. To test the difference in readings from both balances, ten pre-weighted (empty) circles were measured again in the second balance. We found a difference of 0.001 g difference in the reported weights between balances. Post_Bake_Offset_g = The same empty nitex circles were measured before and after baking. We found there was a weight loss by the nitex circle alone of 0.002 g. Adjusted_Dry_Weight_g = This is the weight of the mesozooplankton sample (post-bake minus pre-bake weights, taking offsets into consideration). Net_Split = Initial Sample split from net sample. Final_Aliquot = fraction of the original sample used for this experiment. Begin_Depth_m = depth in meters where the net was opened and started sampling. End_Depth_m = depth in meters where the net was closed and stopped sampling. Depth_Range_m = depth interval sampled by the net between opening and closing interval. Swept_Volume_m3 = water volume (meters cubed) filtered (i.e. sampled) by the net. Dry_Weight_mg_PER_m3 = volumetric mesozooplankton dry weight (milligrams of mesozooplankton dry mass per meter cube). Aerial_Dry_Weight_mg_PER_m2 = aerial mesozooplankton dry weight (milligrams of mesozooplankton dry mass per meter square). Carbon_Mass_ugC_PER_L = volumetric Mesozooplankton Dry Weight converted into volumetric Mesozooplankton Carbon Mass (micrograms of Carbon per liter). Notes = Notes pertaining to collection or laboratory processing.|An attempt was made to "ground truth" CONCORDE mesozooplankton dry weight measurements by comparing them to existing data from the Northern Gulf of Mexico. Since no dry weight measurements were found in publicly available datasets, dry weight data was converted to Carbon Mass (micrograms of Carbon per L) by following Moriarty and O-Brien (2013), and using the equations from Wiebe et al. (1975), and Wiebe (1988). Carbon Mass was then directly compared other measurements (model estimates) available for the region, which includes data from all seasons. For the direct comparison, data from all size classes coming from each sample were pooled together since no size-fractionated Carbon Mass was found.||||These data can be used in conjunction with mesozooplankton gut fluorescence data to get grazing rates estimates.
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Distribution info: Distribution: Distributor: Distributor: Distributor contact: Responsible party: Organisation name: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC) Contact info: Contact: Phone: Telephone: Voice: 3618253604 Address: Address: Delivery point: 6300 Ocean Drive City: Corpus Christi Administrative area: TX Postal code: 78412 Country: USA Electronic mail address: griidc@gomri.org Online Resource: Online Resource: Linkage: URL: https://data.gulfresearchinitiative.org Role: Role code: distributor Distributor format: Format: Name: csv Version: inapplicable File decompression technique: Distributor transfer options: Digital transfer options: Transfer size: 0.0449 Online: Online Resource: Linkage: URL: http://data.gulfresearchinitiative.org/data/R4.x260.000:0027 Protocol: https
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Metadata maintenance: Maintenance information: Maintenance and update frequency: unknown Maintenance note: This ISO metadata record was automatically generated from information provided to GRIIDC for dataset: R4.x260.000:0027 on 2018-12-18T11:40:09+00:00
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