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Mesozooplankton Size-Fractionated Dry Weight, cruise PTS01, Fall 2015Consortium for Oil Spill Exposure Pathways in Coastal River-Dominated Ecosystems (CONCORDE)
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Abstract:
Mesozooplankton samples were taken during the fall of 2015 between October 27 and November 5 aboard R/V Point Sur using a mininess (BIONESS) tow system. Samples were taken from three nets, corresponding to three depth intervals (e.g. bottom, midwater, surface). Nets had 202 micrometer mesh size, but in some cases samples were taken from nets with 0.333 micrometer mesh. Samples were transferred into a bucket immediately after retrieval and carbonated water was added to the sample to stop or avoid further 'net feeding' or regurgitation from individual zooplankton. Typically, each net sample was split in two aliquots using a Folsom Splitter aboard the ship. The 1/2 aliquot separated from the original sample was then split further in two subsamples: one was used to measure mesozooplankton dry weight and the other was processed for gut fluorescence (data reported in GRIIDC UDI: R4.x260.000:0034). When samples were too thick, the initial sample was split into smaller aliquots (clearly indicated in the dataset). The corresponding aliquot was then size fractionated into five classes using sieves with meshes measuring: 0.212, 0.5, 1, 2, and 4.75 mm. Filtered seawater was used during sample split and size fractionation. Next, each size fraction was filtered onto pre-weighted nitex circles (2.5 mm diameter) with a 0.202 mesh size. Nitex circles were individually put in labeled plastic petri dishes and stored stacked inside sealed plastic bags to avoid moisture from getting in touch with the nitex before using them. After fractionation, each sample was poured onto a nitex circle using a filtering system aboard the ship, rinsed with DI water, transferred to its pre-labeled petri dish and frozen immediately after filtering. At the lab, the samples were dried up in an oven at 60 degrees Celsius over a period of 48 hours and weighted immediately after taking each sample out of the oven. The difference between the weight of the filter minus the weight after drying the sample constituted the mesozooplankton dry weight. These data will be used in combination with other mesozooplankton net data to study zooplankton processes in the river-dominated northern Gulf of Mexico.
Purpose:
The dataset was developed to provide mesozooplankton size-fractionated information that will be used, among others, to normalize mesozooplankton grazing rates measured through the gut fluorescence.
Theme Keywords:
Mesozooplankton, Dry Weight, Biomass, Size Fractionated, Mississippi Bight, Continental Shelf, Mobile River Delta
File Format:
csv
Dataset Downloads:
1
Mesozooplankton Size-Fractionated Dry Weight, cruise PTS01, Fall 2015
Identification Information
Distribution Information
Metadata Maintenance Information
Metadata:
File identifier:
R4.x260.000-0026-metadata.xml
Language:
eng; USA
Character set:
Character set code:
utf8
Hierarchy level:
Scope code:
dataset
Metadata author:
Responsible party:
Individual name:
Robert (Bob) Cowen
Organisation name:
Oregon State University / Hatfield Marine Science Center
Position name:
Director
Contact info:
Contact:
Phone:
Telephone:
Voice:
5418670211
Facsimile:
Address:
Address:
Delivery point:
2030 SE Marine Science Dr
City:
Newport
Administrative area:
Oregon
Postal code:
97365
Country:
USA
Electronic mail address:
robert.cowen@oregonstate.edu
Role:
Role code:
pointOfContact
Date stamp:
2018-08-11T13:36:37+00:00
Metadata standard name:
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
Metadata standard version:
ISO 19115-2:2009(E)
Dataset URI:
https://data.gulfresearchinitiative.org/metadata/R4.x260.000:0026
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Identification info:
Data identification:
Citation:
Citation:
Title:
Mesozooplankton Size-Fractionated Dry Weight, cruise PTS01, Fall 2015
Alternate title:
ISIIS Raw Physical Data
Date:
Date:
Date:
2016-10-18
Date type:
Date type code:
creation
Abstract:
Mesozooplankton samples were taken during the fall of 2015 between October 27 and November 5 aboard R/V Point
Sur using a mininess (BIONESS) tow system. Samples were taken from three nets, corresponding to three depth
intervals (e.g. bottom, midwater, surface). Nets had 202 micrometer mesh size, but in some cases samples
were taken from nets with 0.333 micrometer mesh. Samples were transferred into a bucket immediately after
retrieval and carbonated water was added to the sample to stop or avoid further 'net feeding' or
regurgitation from individual zooplankton. Typically, each net sample was split in two aliquots using a
Folsom Splitter aboard the ship. The 1/2 aliquot separated from the original sample was then split further
in two subsamples: one was used to measure mesozooplankton dry weight and the other was processed for gut
fluorescence (data reported in GRIIDC UDI: R4.x260.000:0034). When samples were too thick, the initial
sample was split into smaller aliquots (clearly indicated in the dataset). The corresponding aliquot was
then size fractionated into five classes using sieves with meshes measuring: 0.212, 0.5, 1, 2, and 4.75 mm.
Filtered seawater was used during sample split and size fractionation. Next, each size fraction was filtered
onto pre-weighted nitex circles (2.5 mm diameter) with a 0.202 mesh size. Nitex circles were individually
put in labeled plastic petri dishes and stored stacked inside sealed plastic bags to avoid moisture from
getting in touch with the nitex before using them. After fractionation, each sample was poured onto a nitex
circle using a filtering system aboard the ship, rinsed with DI water, transferred to its pre-labeled petri
dish and frozen immediately after filtering. At the lab, the samples were dried up in an oven at 60 degrees
Celsius over a period of 48 hours and weighted immediately after taking each sample out of the oven. The
difference between the weight of the filter minus the weight after drying the sample constituted the
mesozooplankton dry weight. These data will be used in combination with other mesozooplankton net data to
study zooplankton processes in the river-dominated northern Gulf of Mexico.
Purpose:
The dataset was developed to provide mesozooplankton size-fractionated information that will be used, among
others, to normalize mesozooplankton grazing rates measured through the gut fluorescence.
Status:
Progress code:
completed
Point of contact:
Responsible party:
Individual name:
Christian Briseno-Avena
Organisation name:
Oregon State University / Hatfield Marine Science Center
Position name:
Postdoctoral Researcher
Contact info:
Contact:
Phone:
Telephone:
Voice:
5418670314
Facsimile:
Address:
Address:
Delivery point:
2030 SE Marine Science Dr
City:
Newport
Administrative area:
Oregon
Postal code:
97365
Country:
USA
Electronic mail address:
brisenoc@oregonstate.edu
Role:
Role code:
pointOfContact
Point of contact:
Responsible party:
Individual name:
Robert (Bob) Cowen
Organisation name:
Oregon State University / Hatfield Marine Science Center
Position name:
Director
Contact info:
Contact:
Phone:
Telephone:
Voice:
5418670211
Facsimile:
Address:
Address:
Delivery point:
2030 SE Marine Science Dr
City:
Newport
Administrative area:
Oregon
Postal code:
97365
Country:
USA
Electronic mail address:
robert.cowen@oregonstate.edu
Role:
Role code:
pointOfContact
Descriptive keywords:
Keywords:
Keyword:
Mesozooplankton
Keyword:
Dry Weight
Keyword:
Biomass
Keyword:
Size Fractionated
Keyword:
Mississippi Bight
Keyword:
Continental Shelf
Keyword:
Mobile River Delta
Type:
Keyword type code:
theme
Descriptive keywords:
Keywords:
Keyword:
Gulf of Mexico
Type:
Keyword type code:
place
Language:
eng; USA
Topic category:
Topic category code:
biota
Topic category:
Topic category code:
oceans
Extent:
Extent:
Geographic element:
Geographic bounding box:
West bound longitude:
-88.9712
East bound longitude:
-87.5238
South bound latitude:
29.2581
North bound latitude:
30.1926
Geographic element:
BoundingPolygon:
Polygon:
gml:MultiPoint:
gml:pointMember:
Point:
gml:pos:
30.1287 -88.1237
gml:pointMember:
Point:
gml:pos:
30.1276 -88.1247
gml:pointMember:
Point:
gml:pos:
29.5686 -88.1348
gml:pointMember:
Point:
gml:pos:
30.1926 -87.5295
gml:pointMember:
Point:
gml:pos:
29.664 -87.5238
gml:pointMember:
Point:
gml:pos:
30.1283 -88.1248
gml:pointMember:
Point:
gml:pos:
29.5988 -88.1463
gml:pointMember:
Point:
gml:pos:
30.0915 -88.6133
gml:pointMember:
Point:
gml:pos:
29.5905 -88.597
gml:pointMember:
Point:
gml:pos:
29.2581 -88.803
gml:pointMember:
Point:
gml:pos:
29.344 -88.6425
gml:pointMember:
Point:
gml:pos:
29.3244 -88.9712
gml:pointMember:
Point:
gml:pos:
29.2832 -88.706
Temporal element:
Temporal extent:
Extent:
Time period:
Description:
ground condition
Begin date:
2015-10-29
End date:
2015-11-05
Supplemental Information:
CRUISE_ID = research cruise unique identifier. YEAR = Year sample was collected. Month = Month sample was
collected. Day = Day sample was collected. Time_UTC_HH = hour sample was collected, in UTC. Time_UTC_MM =
minutes when sample was collected, in UTC. Latitude = Latitude (decimal). Longitude = Longitude (decimal).
Negative sign indicate latitude west. Sampling_Type = Name assigned to the transect or location in which the
station took place, usually indicating the type of study. Tow_ID = Unique Net Tow indentifier. MN indicates
it was mininess net. Net_ID = mininess number from which the sample was taken. Petri_Dish_Number = label on
the petri dish for each pre-weighted nitex circle. Mesh_Size_um = mesh size of the net used during tow.
Gear_Type = The gear used for the plankton tow. Size_Fraction_mm = size of the sieve mesh, the values
correspond to, in millimeters. Pre_Bake_Weight_g = weight of the nitex circle alone prior to utilization
(i.e. blank weight). Post_Bake_Weight_g = weight of the nitex circle and mesozooplankton sample combined.
Balance_Offset_g = two different balances were used in some cases. To test the difference in readings from
both balances, ten pre-weighted (empty) circles were measured again in the second balance. We found a
difference of 0.001 g difference in the reported weights between balances. Post_Bake_Offset_g = The same
empty nitex circles were measured before and after baking. We found there was a weight loss by the nitex
circle alone of 0.002 g. Adjusted_Dry_Weight_g = This is the weight of the mesozooplankton sample (post-bake
minus pre-bake weights, taking offsets into consideration). Net_Split = Initial Sample split from net
sample. Final_Aliquot = fraction of the original sample used for this experiment. Begin_Depth_m = depth in
meters where the net was opened and started sampling. End_Depth_m = depth in meters where the net was closed
and stopped sampling. Depth_Range_m = depth interval sampled by the net between opening and closing
interval. Swept_Volume_m3 = water volume (meters cubed) filtered (i.e. sampled) by the net.
Dry_Weight_mg_PER_m3 = volumetric mesozooplankton dry weight (milligrams of mesozooplankton dry mass per
meter cube). Aerial_Dry_Weight_mg_PER_m2 = aerial mesozooplankton dry weight (milligrams of mesozooplankton
dry mass per meter square). Carbon_Mass_ugC_PER_L = volumetric Mesozooplankton Dry Weight converted into
volumetric Mesozooplankton Carbon Mass (micrograms of Carbon per liter). Notes = Notes pertaining to
collection or laboratory processing.|An attempt was made to "ground truth" CONCORDE mesozooplankton dry
weight measurements by comparing them to existing data from the Northern Gulf of Mexico. Since no dry weight
measurements were found in publicly available datasets, dry weight data was converted to Carbon Mass
(micrograms of Carbon per L) by following Moriarty and O-Brien (2013), and using the equations from Wiebe et
al. (1975), and Wiebe (1988). Carbon Mass was then directly compared other measurements (model estimates)
available for the region, which includes data from all seasons. For the direct comparison, data from all
size classes coming from each sample were pooled together since no size-fractionated Carbon Mass was
found.||||These data can be used in conjunction with mesozooplankton gut fluorescence data to get grazing
rates estimates.
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Distribution info:
Distribution:
Distributor:
Distributor:
Distributor contact:
Responsible party:
Organisation name:
Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC)
Contact info:
Contact:
Phone:
Telephone:
Voice:
3618253604
Address:
Address:
Delivery point:
6300 Ocean Drive
City:
Corpus Christi
Administrative area:
TX
Postal code:
78412
Country:
USA
Electronic mail address:
griidc@gomri.org
Online Resource:
Online Resource:
Linkage:
URL:
https://data.gulfresearchinitiative.org
Role:
Role code:
distributor
Distributor format:
Format:
Name:
csv
Version:
inapplicable
File decompression technique:
Distributor transfer options:
Digital transfer options:
Transfer size:
0.0244
Online:
Online Resource:
Linkage:
URL:
http://data.gulfresearchinitiative.org/data/R4.x260.000:0026
Protocol:
https
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Metadata maintenance:
Maintenance information:
Maintenance and update frequency:
unknown
Maintenance note:
This ISO metadata record was automatically generated from information provided to GRIIDC for dataset:
R4.x260.000:0026 on 2018-12-18T10:28:44+00:00
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