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Oxygen consumption and response to hypoxia in Gulf killifish (Fundulus grandis) populations resistant to dioxin-like compounds (DLCs)

Relationship of Effects of Cardiac Outcomes in Fish for Validation of Ecological Risk (RECOVER)

DOI:
10.7266/N78S4MWF
 
UDI:
R4.x258.258:0003
Last Update:
Jun 03 2016 21:04 UTC
 
Dataset Author(s):
Benjamin Dubansky
Point of Contact:
Dubansky, Benjamin
University of North Texas / Department of Biological Sciences
1511 West Sycamore Street Life Sciences Complex
Denton, TX  76203
USA
benjamin.dubansky@unt.edu
Funding Source:
RFP-IV
Data Collection Period:
2015-01-01 to 2016-01-01

Identified Submitted Metadata Available
3 3 3 3

Abstract:

This dataset is comprised of data that can be used to determine resting metabolic rate and response to hypoxia in in four populations of Gulf killifish (Fundulus grandis) embryos. Two of these populations have been identified to be resistant to cardiac teratogenicity (Vince Bayou - VB and Paul bayou - PB) associated with exposure to polyaromatic hydrocarbons (PAHs) Two reference populations (Gang's bayou - GB and Smith Point - SP)were utilized for comparison. All data is in spreadsheet format. Declining oxygen, as organisms consumed oxygen, are reported for each population in millimoles oxygen in each 3 ml chamber. Mass and times of measurement are reported for calculation of metabolic rate as the rate of oxygen consumption per mass of each animal per unit time. Response to hypoxia is reported as time until larvae lost equilibrium during exposure to severe hypoxia. Response to hypoxia can be calculated as the time until loss of equilibrium per mass of the animal. Mass of larvae is reported to standardize for size differences in size.

Purpose:

This dataset was produced to determine metabolic rates of larval fish from different populations and to determine the response to acute low oxygen (hypoxia).

Theme Keywords:

hypoxia, metabolism, killifish, development, fundulus, resistance

File Format:

xml

Oxygen consumption and response to hypoxia in Gulf killifish (Fundulus grandis) populations resistant to dioxin-like compounds (DLCs)



Identification Information
Distribution Information
Metadata Maintenance Information

Metadata: 
  File identifier: 
      R4.x258.258-0003-metadata.xml
  Language: 
      eng; USA
  Character set: 
    Character set code: 
      utf8
  Hierarchy level: 
    Scope code: 
      dataset
  Metadata author: 
    Responsible party: 
      Individual name: 
          Benjamin Dubansky
      Organisation name: 
          University of North Texas
      Position name: 
          Research Scientist
      Contact info: 
        Contact: 
          Phone: 
            Telephone: 
              Voice: 
                  225-931-2937
              Facsimile: 
          Address: 
            Address: 
              Delivery point: 
                  1511 West Sycamore Street
                  Life Sciences Complex
              City: 
                  Denton
              Administrative area: 
                  TX
              Postal code: 
                  76203
              Country: 
                  USA
              Electronic mail address: 
                  benjamin.dubansky@unt.edu
      Role: 
        Role code: 
          pointOfContact
  Metadata author: 
    Responsible party: 
      Individual name: 
          Madeline Munoz-Bustamante
      Organisation name: 
          University of Miami
      Position name: 
          Data Manager
      Contact info: 
        Contact: 
          Phone: 
            Telephone: 
              Voice: 
              Facsimile: 
          Address: 
            Address: 
              Delivery point: 
                  4600 Rickenbacker Causeway
              City: 
                  Miami
              Administrative area: 
                  FL
              Postal code: 
                  33149
              Country: 
                  USA
              Electronic mail address: 
                  mbustamante@rsmas.miami.edu
      Role: 
        Role code: 
          pointOfContact
  Date stamp: 
      2016-06-06T08:55:55-05:00
  Metadata standard name: 
      ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
  Metadata standard version: 
      ISO 19115-2:2009(E)
  Dataset URI: 
      https://data.gulfresearchinitiative.org/metadata/R4.x258.258:0003
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Identification info: Data identification: Citation: Citation: Title: Oxygen consumption and response to hypoxia in Gulf killifish (Fundulus grandis) populations resistant to dioxin-like compounds (DLCs) Alternate title: Date: Date: Date: 2016-06-03 Date type: Date type code: publication Abstract: This dataset is comprised of data that can be used to determine resting metabolic rate and response to hypoxia in in four populations of Gulf killifish (Fundulus grandis) embryos. Two of these populations have been identified to be resistant to cardiac teratogenicity (Vince Bayou - VB and Paul bayou - PB) associated with exposure to polyaromatic hydrocarbons (PAHs) Two reference populations (Gang's bayou - GB and Smith Point - SP)were utilized for comparison. All data is in spreadsheet format. Declining oxygen, as organisms consumed oxygen, are reported for each population in millimoles oxygen in each 3 ml chamber. Mass and times of measurement are reported for calculation of metabolic rate as the rate of oxygen consumption per mass of each animal per unit time. Response to hypoxia is reported as time until larvae lost equilibrium during exposure to severe hypoxia. Response to hypoxia can be calculated as the time until loss of equilibrium per mass of the animal. Mass of larvae is reported to standardize for size differences in size. Purpose: This dataset was produced to determine metabolic rates of larval fish from different populations and to determine the response to acute low oxygen (hypoxia). Status: Progress code: completed Point of contact: Responsible party: Individual name: Benjamin Dubansky Organisation name: University of North Texas Position name: Research Scientist Contact info: Contact: Phone: Telephone: Voice: 225-931-2937 Facsimile: Address: Address: Delivery point: 1511 West Sycamore Street Life Sciences Complex City: Denton Administrative area: TX Postal code: 76203 Country: USA Electronic mail address: benjamin.dubansky@unt.edu Role: Role code: pointOfContact Point of contact: Responsible party: Individual name: Madeline Munoz-Bustamante Organisation name: University of Miami Position name: Data Manager Contact info: Contact: Phone: Telephone: Voice: Facsimile: Address: Address: Delivery point: 4600 Rickenbacker Causeway City: Miami Administrative area: FL Postal code: 33149 Country: USA Electronic mail address: mbustamante@rsmas.miami.edu Role: Role code: pointOfContact Descriptive keywords: Keywords: Keyword: hypoxia Keyword: metabolism Keyword: killifish Keyword: development Keyword: fundulus Keyword: resistance Type: Keyword type code: theme Descriptive keywords: Keywords: Keyword: Texas Keyword: Galveston Type: Keyword type code: place Language: eng; USA Topic category: Topic category code: biota Topic category: Topic category code: environment Extent: Extent: Geographic element: Geographic bounding box: West bound longitude: -95.22048 East bound longitude: -94.78559 South bound latitude: 29.25843 North bound latitude: 29.72823 Geographic element: BoundingPolygon: Polygon: gml:MultiPoint: gml:pointMember: Point: gml:pos: 29.54368 -94.78559 gml:pointMember: Point: gml:pos: 29.25843 -94.9125 gml:pointMember: Point: gml:pos: 29.71944 -95.22048 gml:pointMember: Point: gml:pos: 29.72823 -95.11403 Temporal element: Temporal extent: Extent: Time period: Description: ground condition Begin date: 2015-01-01 End date: 2016-01-01 Supplemental Information: Plate ID; Well; Population (Pop, site abbreviation); Oxygen content (millimoles); Mass (WM (wet mass), DM (dry mass), grams or milligrams); Time (seconds and minutes); Loss of equilibrium (LOE) Sampling site (abbreviation) latitude (decimal degrees) longitude (decimal degrees) Smith Point (SP) 29.543683 -94.785589 Gangs Bayou (GB) 29.258428 -94.9125 Vince Bayou (VB) 29.719444 -95.220481 Patrick Bayou (PB) 29.728233 -95.114031 |Oxygen was monitored using a Sensor Dish Reader to detect oxygen saturation in 24 well oxygen sensor plates (Precision Sensing GmbH). Briefly, fish were placed in each well, the wells were sealed, then oxygen content in the wells was recorded over time, as the fish consumed oxygen. Loss of equilibrium experiments were conducted in 1 ml glass vials stoppered with a ball of wax. Briefly, fish were put in the glass vials and sealed. After time, the fish began to lose equilibrium and lost the ability to right itself. As the fish began to turn upside down or sideways, the vial was inverted three times, with 5 seconds between each inversion. If the fish was unable to right itself after three inversions, the time was noted. |From Precision Sensing Gmbh - Germany SDR Sensor Dish Reader OxoDish - OD24 - 24-well plate for measuring oxygen on the SDR SDR v38 Software - Software for data acquisition |||
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Distribution info: Distribution: Distributor: Distributor: Distributor contact: Responsible party: Organisation name: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC) Position name: Contact info: Contact: Phone: Telephone: Voice: +1-361-825-3604 Facsimile: +1-361-825-2050 Address: Address: Delivery point: 6300 Ocean Drive Unit 5869 City: Corpus Christi Administrative area: Texas Postal code: 78412-5869 Country: USA Electronic mail address: griidc@gomri.org Online Resource: Online Resource: Linkage: URL: http://data.gulfresearchinitiative.org Protocol: Role: Role code: distributor Distributor format: Format: Name: xml Version: inapplicable File decompression technique: Distributor transfer options: Digital transfer options: Transfer size: 0.4 Online: Online Resource: Linkage: URL: https://data.gulfresearchinitiative.org/data/R4.x258.258:0003 Protocol:
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Metadata maintenance: Maintenance information: Maintenance and update frequency: unknown Maintenance note: This ISO metadata record was automatically generated by the GRIIDC Metadata Generator from DIF and Registry information provided to GRIIDC for dataset: R4.x258.258:0003 on 2016-06-03T18:10:32+00:00 Maintenance note: This ISO metadata record was created using the 'Check and Save to File' (with form validation) function of the GRIIDC ISO 19115-2 Metadata Editor on 2016-06-03T18:33:45+00:00
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