Oxygen consumption and response to hypoxia in Gulf killifish (Fundulus grandis) populations resistant to dioxin-like compounds (DLCs)

Relationship of Effects of Cardiac Outcomes in Fish for Validation of Ecological Risk (RECOVER)

DOI:
10.7266/N78S4MWF
 
UDI:
R4.x258.258:0003
Last Update:
Jun 03 2016 21:04 UTC
 
Dataset Author(s):
Benjamin Dubansky
Point of Contact:
Munoz-Bustamante, Madeline
University of Miami / Rosenstiel School of Marine and Atmospheric Science
4600 Rickenbacker Cswy
Miami, Florida  33149
USA
mbustamante@rsmas.miami.edu
Funding Source:
RFP-IV
Data Collection Period:
2015-01-01 to 2016-01-01

Identified Submitted In-Review Available
3 3 3 3

Suggested Citation:

Benjamin Dubansky. 2016. Oxygen consumption and response to hypoxia in Gulf killifish (Fundulus grandis) populations resistant to dioxin-like compounds (DLCs). Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N78S4MWF

Abstract:

This dataset is comprised of data that can be used to determine resting metabolic rate and response to hypoxia in in four populations of Gulf killifish (Fundulus grandis) embryos. Two of these populations have been identified to be resistant to cardiac teratogenicity (Vince Bayou - VB and Paul bayou - PB) associated with exposure to polyaromatic hydrocarbons (PAHs) Two reference populations (Gang's bayou - GB and Smith Point - SP)were utilized for comparison. All data is in spreadsheet format. Declining oxygen, as organisms consumed oxygen, are reported for each population in millimoles oxygen in each 3 ml chamber. Mass and times of measurement are reported for calculation of metabolic rate as the rate of oxygen consumption per mass of each animal per unit time. Response to hypoxia is reported as time until larvae lost equilibrium during exposure to severe hypoxia. Response to hypoxia can be calculated as the time until loss of equilibrium per mass of the animal. Mass of larvae is reported to standardize for size differences in size.

Purpose:

This dataset was produced to determine metabolic rates of larval fish from different populations and to determine the response to acute low oxygen (hypoxia).

Theme Keywords:

hypoxia, metabolism, killifish, development, fundulus, resistance

File Format:

xml

Filename:

OxygenSaturation-TimetoLOE.xml (312.75 KB)

Dataset Downloads:

4

Oxygen consumption and response to hypoxia in Gulf killifish (Fundulus grandis) populations resistant to dioxin-like compounds (DLCs)



Identification Information
Distribution Information
Metadata Maintenance Information

Metadata: 
  File identifier: 
      R4.x258.258-0003-metadata.xml
  Language: 
      eng; USA
  Character set: 
    Character set code: 
      utf8
  Hierarchy level: 
    Scope code: 
      dataset
  Metadata author: 
    Responsible party: 
      Individual name: 
          Benjamin Dubansky
      Organisation name: 
          University of North Texas / Department of Biological Sciences
      Position name: 
      Contact info: 
        Contact: 
          Phone: 
            Telephone: 
              Voice: 
                  2259312937
              Facsimile: 
          Address: 
            Address: 
              Delivery point: 
                  1511 West Sycamore Street
                  Life Sciences Complex
              City: 
                  Denton
              Administrative area: 
                  TX
              Postal code: 
                  76203
              Country: 
                  USA
              Electronic mail address: 
                  benjamin.dubansky@unt.edu
      Role: 
        Role code: 
          pointOfContact
  Date stamp: 
      2021-05-04T20:04:56+00:00
  Metadata standard name: 
      ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
  Metadata standard version: 
      ISO 19115-2:2009(E)
  Dataset URI: 
      https://data.gulfresearchinitiative.org/metadata/R4.x258.258:0003
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Identification info: Data identification: Citation: Citation: Title: Oxygen consumption and response to hypoxia in Gulf killifish (Fundulus grandis) populations resistant to dioxin-like compounds (DLCs) Alternate title: Date: Date: Date: 2016-06-06 Date type: Date type code: publication Identifier: Identifier: Code: Anchor: xlink: https://dx.doi.org/10.7266/N78S4MWF title: DOI doi:10.7266/N78S4MWF Abstract: This dataset is comprised of data that can be used to determine resting metabolic rate and response to hypoxia in in four populations of Gulf killifish (Fundulus grandis) embryos. Two of these populations have been identified to be resistant to cardiac teratogenicity (Vince Bayou - VB and Paul bayou - PB) associated with exposure to polyaromatic hydrocarbons (PAHs) Two reference populations (Gang's bayou - GB and Smith Point - SP)were utilized for comparison. All data is in spreadsheet format. Declining oxygen, as organisms consumed oxygen, are reported for each population in millimoles oxygen in each 3 ml chamber. Mass and times of measurement are reported for calculation of metabolic rate as the rate of oxygen consumption per mass of each animal per unit time. Response to hypoxia is reported as time until larvae lost equilibrium during exposure to severe hypoxia. Response to hypoxia can be calculated as the time until loss of equilibrium per mass of the animal. Mass of larvae is reported to standardize for size differences in size. Purpose: This dataset was produced to determine metabolic rates of larval fish from different populations and to determine the response to acute low oxygen (hypoxia). Status: Progress code: completed Point of contact: Responsible party: Individual name: Madeline Munoz-Bustamante Organisation name: University of Miami / Rosenstiel School of Marine and Atmospheric Science Position name: Program Manager Contact info: Contact: Phone: Telephone: Voice: Facsimile: Address: Address: Delivery point: 4600 Rickenbacker Cswy City: Miami Administrative area: Florida Postal code: 33149 Country: USA Electronic mail address: mbustamante@rsmas.miami.edu Role: Role code: pointOfContact Point of contact: Responsible party: Individual name: Benjamin Dubansky Organisation name: University of North Texas / Department of Biological Sciences Position name: Contact info: Contact: Phone: Telephone: Voice: 2259312937 Facsimile: Address: Address: Delivery point: 1511 West Sycamore Street Life Sciences Complex City: Denton Administrative area: TX Postal code: 76203 Country: USA Electronic mail address: benjamin.dubansky@unt.edu Role: Role code: pointOfContact Descriptive keywords: Keywords: Keyword: hypoxia Keyword: metabolism Keyword: killifish Keyword: development Keyword: fundulus Keyword: resistance Type: Keyword type code: theme Descriptive keywords: Keywords: Keyword: Texas Keyword: Galveston Type: Keyword type code: place Resource constraints: title: Cite As Constraints: Use limitation: Benjamin Dubansky. 2016. Oxygen consumption and response to hypoxia in Gulf killifish (Fundulus grandis) populations resistant to dioxin-like compounds (DLCs). Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N78S4MWF Resource constraints: title: CC0 License Legal constraints: Use constraints: Restriction code: licenceUnrestricted Other constraints: This information is released under the Creative Commons license - No Rights Reserved - CC0 1.0 Universal (https://creativecommons.org/publicdomain/zero/1.0/). The person who associated a work with this deed has dedicated the work to the public domain by waiving all of his or her rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law. You can copy, modify, distribute and perform the work, even for commercial purposes, all without asking permission. Resource constraints: title: Liability and Warranty Legal constraints: Other constraints: All materials on this website are made available to GRIIDC and in turn to you "as-is." Content may only be submitted by an individual who represents and warrants that s/he has sufficient rights to be able to make the content available under a CC0 waiver. There is no warranty (expressed or implied) to these materials, their title, accuracy, non-infringement of third party rights, or fitness for any particular purpose, including the performance or results you may obtain from their use. Use these materials at your own risk. Under no circumstances shall GRIIDC be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use or the inability to use either this website or the materials available via this website. If you are dissatisfied with any website feature, content, or terms of use, your sole and exclusive remedy is to discontinue use. Aggregation Info: AggregateInformation: Aggregate Data Set Name: title: Related Publication Citation Citation: Title: Oziolor, E. M., Dubansky, B., Burggren, W. W., & Matson, C. W. (2016). Cross-resistance in Gulf killifish (Fundulus grandis) populations resistant to dioxin-like compounds. Aquatic Toxicology, 175, 222–231. doi:10.1016/j.aquatox.2016.03.019 Date: inapplicable Aggregate Data Set Identifier: title: Related Publication DOI Identifier: Code: Anchor: xlink: https://dx.doi.org/10.1016/j.aquatox.2016.03.019 title: DOI doi:10.1016/j.aquatox.2016.03.019 Association Type: Association type code: crossReference Language: eng; USA Topic category: Topic category code: biota Topic category: Topic category code: environment Extent: Extent: Geographic element: Geographic bounding box: West bound longitude: -95.22048 East bound longitude: -94.78559 South bound latitude: 29.25843 North bound latitude: 29.72823 Geographic element: BoundingPolygon: Polygon: gml:MultiPoint: gml:pointMember: Point: gml:pos: 29.54368 -94.78559 gml:pointMember: Point: gml:pos: 29.25843 -94.9125 gml:pointMember: Point: gml:pos: 29.71944 -95.22048 gml:pointMember: Point: gml:pos: 29.72823 -95.11403 Temporal element: Temporal extent: Extent: Time period: Description: ground condition Begin date: 2015-01-01 End date: 2016-01-01 Supplemental Information: Plate ID; Well; Population (Pop, site abbreviation); Oxygen content (millimoles); Mass (WM (wet mass), DM (dry mass), grams or milligrams); Time (seconds and minutes); Loss of equilibrium (LOE) Sampling site (abbreviation) latitude (decimal degrees) longitude (decimal degrees) Smith Point (SP) 29.543683 -94.785589 Gangs Bayou (GB) 29.258428 -94.9125 Vince Bayou (VB) 29.719444 -95.220481 Patrick Bayou (PB) 29.728233 -95.114031 |Oxygen was monitored using a Sensor Dish Reader to detect oxygen saturation in 24 well oxygen sensor plates (Precision Sensing GmbH). Briefly, fish were placed in each well, the wells were sealed, then oxygen content in the wells was recorded over time, as the fish consumed oxygen. Loss of equilibrium experiments were conducted in 1 ml glass vials stoppered with a ball of wax. Briefly, fish were put in the glass vials and sealed. After time, the fish began to lose equilibrium and lost the ability to right itself. As the fish began to turn upside down or sideways, the vial was inverted three times, with 5 seconds between each inversion. If the fish was unable to right itself after three inversions, the time was noted. |From Precision Sensing Gmbh - Germany SDR Sensor Dish Reader OxoDish - OD24 - 24-well plate for measuring oxygen on the SDR SDR v38 Software - Software for data acquisition |||
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Distribution info: Distribution: Distributor: Distributor: Distributor contact: Responsible party: Organisation name: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC) Contact info: Contact: Phone: Telephone: Voice: 3618253604 Address: Address: Delivery point: 6300 Ocean Drive City: Corpus Christi Administrative area: TX Postal code: 78412 Country: USA Electronic mail address: griidc@gomri.org Online Resource: Online Resource: Linkage: URL: https://data.gulfresearchinitiative.org Role: Role code: distributor Distributor format: Format: Name: xml Version: inapplicable File decompression technique: Distributor transfer options: Digital transfer options: Transfer size: 0.3127 Online: Online Resource: Linkage: URL: https://data.gulfresearchinitiative.org/data/R4.x258.258:0003 Protocol: https Name: Data Landing Page Description: GRIIDC dataset landing page Function: Online function code: information
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Metadata maintenance: Maintenance information: Maintenance and update frequency: unknown Maintenance note: This ISO metadata record was automatically generated from information provided to GRIIDC for dataset: R4.x258.258:0003 on 2021-10-20T11:46:21-05:00
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