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Dataset for: Cross-resistance to pesticides and pro-oxidants in Gulf killifish (Fundulus grandis) populations resistant to dioxin-like compounds (DLCs)

Relationship of Effects of Cardiac Outcomes in Fish for Validation of Ecological Risk (RECOVER)

DOI:
10.7266/N7513W6W
 
UDI:
R4.x258.258:0002
Last Update:
Jun 03 2016 19:06 UTC
 
Dataset Author(s):
Elias Oziolor, Benjamin Dubansky, Warren Burggren and Cole Matson
Point of Contact:
Dubansky, Benjamin
University of North Texas / Department of Biological Sciences
1511 West Sycamore Street Life Sciences Complex
Denton, TX  76203
USA
benjamin.dubansky@unt.edu
Funding Source:
RFP-IV
Data Collection Period:
2014-01-01 to 2016-03-01

Identified Submitted Metadata Available
3 3 3 3

Abstract:

This dataset is comprised of toxicity and biochemical and morphological assays that describe properties of resistance to dioxin-like compounds (DLCs) in four populations of Gulf killifish (Fundulus grandis) embryos. Two of these populations (Vince Bayou - VB and Paul's Bayou - PB) have been identified to be resistant to cardiac teratogenicity associated with exposure to polyaromatic hydrocarbons (PAHs.) Two reference populations were utilized for comparison (Gang's Bayou - GB and Smith Bayou -SP). Embyros were assessed for cross-resistance to pesticides (pyrethroid (permethrin), and carbamate (carbaryl)) and two model pro-oxidants (tert-butyl hydroquinone (tBHQ) and tert-butyl hydroperoxide (tBOOH)). Endpoints are mortality, degree of cardiac deformity, and the ethoxyresorufin-O-deethylase (EROD) assay to establish downregulation of cytochrome P450 1A as an indication of aryl-hydrocarbon receptor recalcitrance in resistant embryos. Morphological assays score the degree of heart deformities in embryos exposed to PCB-126.

Purpose:

The purpose was to determine if these populations are cross-resistant to both PAHs and oxidative stress, since there is much oxidative potential in the resistant populations (VB and PB.)

Theme Keywords:

pro-oxidant, oxidative stress, resistance, killifish, fundulus, EROD, toxicity, adaptation

File Format:

xml

Dataset for: Cross-resistance to pesticides and pro-oxidants in Gulf killifish (Fundulus grandis) populations resistant to dioxin-like compounds (DLCs)



Identification Information
Distribution Information
Metadata Maintenance Information

Metadata: 
  File identifier: 
      R4.x258.258-0002-metadata.xml
  Language: 
      eng; USA
  Character set: 
    Character set code: 
      utf8
  Hierarchy level: 
    Scope code: 
      dataset
  Metadata author: 
    Responsible party: 
      Individual name: 
          Benjamin Dubansky
      Organisation name: 
          University of North Texas
      Position name: 
          Research Scientist
      Contact info: 
        Contact: 
          Phone: 
            Telephone: 
              Voice: 
                  225-931-2937
              Facsimile: 
          Address: 
            Address: 
              Delivery point: 
                  1511 West Sycamore Street
                  Life Sciences Complex
              City: 
                  Denton
              Administrative area: 
                  TX
              Postal code: 
                  76203
              Country: 
                  USA
              Electronic mail address: 
                  benjamin.dubansky@unt.edu
      Role: 
        Role code: 
          pointOfContact
  Metadata author: 
    Responsible party: 
      Individual name: 
          Madeline Munoz-Bustamante
      Organisation name: 
          University of Miami
      Position name: 
          Data Manager
      Contact info: 
        Contact: 
          Phone: 
            Telephone: 
              Voice: 
              Facsimile: 
          Address: 
            Address: 
              Delivery point: 
                  4600 Rickenbacker Causeway
              City: 
                  Miami
              Administrative area: 
                  FL
              Postal code: 
                  33149
              Country: 
                  USA
              Electronic mail address: 
                  mbustamante@rsmas.miami.edu
      Role: 
        Role code: 
          pointOfContact
  Date stamp: 
      2016-06-03T16:20:40-05:00
  Metadata standard name: 
      ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
  Metadata standard version: 
      ISO 19115-2:2009(E)
  Dataset URI: 
      https://data.gulfresearchinitiative.org/metadata/R4.x258.258:0002
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Identification info: Data identification: Citation: Citation: Title: Dataset for: Cross-resistance to pesticides and pro-oxidants in Gulf killifish (Fundulus grandis) populations resistant to dioxin-like compounds (DLCs) Alternate title: Date: Date: Date: 2016-06-03 Date type: Date type code: publication Abstract: This dataset is comprised of toxicity and biochemical and morphological assays that describe properties of resistance to dioxin-like compounds (DLCs) in four populations of Gulf killifish (Fundulus grandis) embryos. Two of these populations (Vince Bayou - VB and Paul's Bayou - PB) have been identified to be resistant to cardiac teratogenicity associated with exposure to polyaromatic hydrocarbons (PAHs.) Two reference populations were utilized for comparison (Gang's Bayou - GB and Smith Bayou -SP). Embyros were assessed for cross-resistance to pesticides (pyrethroid (permethrin), and carbamate (carbaryl)) and two model pro-oxidants (tert-butyl hydroquinone (tBHQ) and tert-butyl hydroperoxide (tBOOH)). Endpoints are mortality, degree of cardiac deformity, and the ethoxyresorufin-O-deethylase (EROD) assay to establish downregulation of cytochrome P450 1A as an indication of aryl-hydrocarbon receptor recalcitrance in resistant embryos. Morphological assays score the degree of heart deformities in embryos exposed to PCB-126. Purpose: The purpose was to determine if these populations are cross-resistant to both PAHs and oxidative stress, since there is much oxidative potential in the resistant populations (VB and PB.) Status: Progress code: completed Point of contact: Responsible party: Individual name: Benjamin Dubansky Organisation name: University of North Texas Position name: Research Scientist Contact info: Contact: Phone: Telephone: Voice: 225-931-2937 Facsimile: Address: Address: Delivery point: 1511 West Sycamore Street Life Sciences Complex City: Denton Administrative area: TX Postal code: 76203 Country: USA Electronic mail address: benjamin.dubansky@unt.edu Role: Role code: pointOfContact Point of contact: Responsible party: Individual name: Madeline Munoz-Bustamante Organisation name: University of Miami Position name: Data Manager Contact info: Contact: Phone: Telephone: Voice: Facsimile: Address: Address: Delivery point: 4600 Rickenbacker Causeway City: Miami Administrative area: FL Postal code: 33149 Country: USA Electronic mail address: mbustamante@rsmas.miami.edu Role: Role code: pointOfContact Descriptive keywords: Keywords: Keyword: pro-oxidant Keyword: oxidative stress Keyword: resistance Keyword: killifish Keyword: fundulus Keyword: EROD Keyword: toxicity Keyword: adaptation Type: Keyword type code: theme Descriptive keywords: Keywords: Keyword: Texas Keyword: Galveston Type: Keyword type code: place Language: eng; USA Topic category: Topic category code: biota Topic category: Topic category code: environment Extent: Extent: Geographic element: Geographic bounding box: West bound longitude: -95.22048 East bound longitude: -94.78559 South bound latitude: 29.25843 North bound latitude: 29.72823 Geographic element: BoundingPolygon: Polygon: gml:MultiPoint: gml:pointMember: Point: gml:pos: 29.54368 -94.78559 gml:pointMember: Point: gml:pos: 29.25843 -94.9125 gml:pointMember: Point: gml:pos: 29.71944 -95.22048 gml:pointMember: Point: gml:pos: 29.72823 -95.11403 Temporal element: Temporal extent: Extent: Time period: Description: ground condition Begin date: 2014-01-01 End date: 2016-03-01 Supplemental Information: Permethrin: Concentration (mass per unit volume); Mortality (percent mortality); Population (sampling population by site) tBHQ: tert-butyl hydroquinone (tBHQ) Concentration (mass per unit volume); Mortality (percent mortality); Population (sampling population by site) tBOOH: Model pro-oxidant tert-butyl hydroperoxide (tBOOH) Concentration (millimoles); Mortality (percent mortality); Population (sampling population by site) EROD: Dose (mL); EROD (fluorescence intensity of the urinary bladder, after background fluorescence was subtracted); Fluorescence intensity was box cox transformed for analysis. Population (sampling population by site) Cardiac Deformity: Dose (mL); Cardiac deformity index (scored quantitatively as 0=not deformed, 1=mildly deformed and 2=severely deformed); Population (sampling population by site); Rank Sampling site (abbreviation) latitude (decimal degrees) longitude (decimal degrees) Smith Point (SP) 29.543683 -94.785589 Gangs Bayou (GB) 29.258428 -94.9125 Vince Bayou (VB) 29.719444 -95.220481 Patrick Bayou (PB) 29.728233 -95.114031|Methods were performed as described in Oziolor et al. 2016 (http://dx.doi.org/10.1016/j.aquatox.2016.03.019)||||
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Distribution info: Distribution: Distributor: Distributor: Distributor contact: Responsible party: Organisation name: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC) Position name: Contact info: Contact: Phone: Telephone: Voice: +1-361-825-3604 Facsimile: +1-361-825-2050 Address: Address: Delivery point: 6300 Ocean Drive Unit 5869 City: Corpus Christi Administrative area: Texas Postal code: 78412-5869 Country: USA Electronic mail address: griidc@gomri.org Online Resource: Online Resource: Linkage: URL: http://data.gulfresearchinitiative.org Protocol: Role: Role code: distributor Distributor format: Format: Name: xml Version: inapplicable File decompression technique: Distributor transfer options: Digital transfer options: Transfer size: 0.1 Online: Online Resource: Linkage: URL: https://data.gulfresearchinitiative.org/data/R4.x258.258:0002 Protocol:
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Metadata maintenance: Maintenance information: Maintenance and update frequency: unknown Maintenance note: This ISO metadata record was automatically generated by the GRIIDC Metadata Generator from DIF and Registry information provided to GRIIDC for dataset: R4.x258.258:0002 on 2016-06-03T18:39:09+00:00 Maintenance note: This ISO metadata record was created using the 'Check and Save to File' (with form validation) function of the GRIIDC ISO 19115-2 Metadata Editor on 2016-06-03T19:04:57+00:00
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