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The effects of oil exposure on cardiac endpoints in red drum 48 hours post-fertilization

Relationship of Effects of Cardiac Outcomes in Fish for Validation of Ecological Risk (RECOVER)

DOI:
10.7266/N7H41PGC
 
UDI:
R4.x258.254:0001
Last Update:
Nov 18 2016 17:47 UTC
 
Dataset Author(s):
Alexis Khursigara
Point of Contact:
Esbaugh, Andrew
The University of Texas at Austin / Marine Science Institute
College of Natural Sciences 750 Channel View Drive
Port Aransas, Texas  78373
USA
a.esbaugh@austin.utexas.edu
Funding Source:
RFP-IV
 
Extent Description:
No field sampling involved.

Identified Submitted Metadata Available
3 3 3 3

Abstract:

The data collected in this study was looking at the influence of weathered and non-weathered oil on larval red drum (Sciaenops ocellatus). Dose responses for weathered and non-weathered oil was conducted on 12 hpf (hours post fertilization) larvae for 72 hours where survival was assessed. Another weathered oil dose response was conducted on 1 hpf larvae for 72 hours where survival was assessed. To examine sub-lethal impacts, 12 hpf larvae were placed into a weathered oil dose response for 48 hours where still images and 11 second videos were taken. Finally, a survival comparison between a filtered and unfiltered weathered oil study was conducted on 12 hpf larvae for 72 hours.

Purpose:

The purpose of this was to determine lethal and sub-lethal impacts of weathered and non-weathered oil on red drum larvae.

Theme Keywords:

Oil Spill Science, Cardiac Function, Polycyclic aromatic hydrocarbons, Larvae, Red Drum, Larval Red Drum, Sciaenops ocellatus

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The effects of oil exposure on cardiac endpoints in red drum 48 hours post-fertilization



Identification Information
Distribution Information
Metadata Maintenance Information

Metadata: 
  File identifier: 
      R4.x258.254-0001-metadata.xml
  Language: 
      eng; USA
  Character set: 
    Character set code: 
      utf8
  Hierarchy level: 
    Scope code: 
      dataset
  Metadata author: 
    Responsible party: 
      Individual name: 
          Andrew Esbaugh
      Organisation name: 
          The University of Texas at Austin
      Position name: 
          Assistant Professor
      Contact info: 
        Contact: 
          Phone: 
            Telephone: 
              Voice: 
                  3617496835
              Facsimile: 
          Address: 
            Address: 
              Delivery point: 
                  750 Channel View Dr
              City: 
                  Port Aransas
              Administrative area: 
                  Texas
              Postal code: 
                  78373
              Country: 
                  USA
              Electronic mail address: 
                  a.esbaugh@austin.utexas.edu
      Role: 
        Role code: 
          pointOfContact
  Metadata author: 
    Responsible party: 
      Individual name: 
          Alexis Khursigara
      Organisation name: 
          The University of Texas at Austin / Marine Science Institute
      Position name: 
          Graduate Student
      Contact info: 
        Contact: 
          Phone: 
            Telephone: 
              Voice: 
                  3617496835
              Facsimile: 
          Address: 
            Address: 
              Delivery point: 
                  750 Channel View Dr
              City: 
                  Port Aransas
              Administrative area: 
                  Texas
              Postal code: 
                  78373
              Country: 
                  USA
              Electronic mail address: 
                  akhursigara@utexas.edu
      Role: 
        Role code: 
          author
  Date stamp: 
      2016-10-12T14:56:10+00:00
  Metadata standard name: 
      ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
  Metadata standard version: 
      ISO 19115-2:2009(E)
  Dataset URI: 
      https://data.gulfresearchinitiative.org/metadata/R4.x258.254:0001
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Identification info: Data identification: Citation: Citation: Title: The effects of oil exposure on cardiac endpoints in red drum 48 hours post-fertilization Alternate title: Date: Date: Date: 2016-10-12 Date type: Date type code: publication Abstract: The data collected in this study was looking at the influence of weathered and non-weathered oil on larval red drum (Sciaenops ocellatus). Dose responses for weathered and non-weathered oil was conducted on 12 hpf (hours post fertilization) larvae for 72 hours where survival was assessed. Another weathered oil dose response was conducted on 1 hpf larvae for 72 hours where survival was assessed. To examine sub-lethal impacts, 12 hpf larvae were placed into a weathered oil dose response for 48 hours where still images and 11 second videos were taken. Finally, a survival comparison between a filtered and unfiltered weathered oil study was conducted on 12 hpf larvae for 72 hours. Purpose: The purpose of this was to determine lethal and sub-lethal impacts of weathered and non-weathered oil on red drum larvae. Status: Progress code: completed Point of contact: Responsible party: Individual name: Andrew Esbaugh Organisation name: The University of Texas at Austin Position name: Assistant Professor Contact info: Contact: Phone: Telephone: Voice: 3617496835 Facsimile: Address: Address: Delivery point: 750 Channel View Dr City: Port Aransas Administrative area: Texas Postal code: 78373 Country: USA Electronic mail address: a.esbaugh@austin.utexas.edu Role: Role code: pointOfContact Point of contact: Responsible party: Individual name: Alexis Khursigara Organisation name: The University of Texas at Austin / Marine Science Institute Position name: Graduate Student Contact info: Contact: Phone: Telephone: Voice: 3617496795 Facsimile: Address: Address: Delivery point: 750 Channel View Dr City: Port Aransas Administrative area: Texas Postal code: 78373 Country: USA Electronic mail address: akhursigara@utexas.edu Role: Role code: author Descriptive keywords: Keywords: Keyword: Oil Spill Science Keyword: Cardiac Function Keyword: Polycyclic aromatic hydrocarbons Keyword: Larvae Keyword: Red Drum Keyword: Larval Red Drum Keyword: Sciaenops ocellatus Type: Keyword type code: theme Descriptive keywords: Keywords: Keyword: inapplicable Type: Keyword type code: place Language: eng; USA Topic category: Topic category code: biota Extent: Extent: Description: No field sampling involved. Supplemental Information: Species, Condition, Replicate, Fish number, Video names, Cardiac chambers, Contractility state, Event number, Area (mm), Perimeter (mm), Major axe ellipse (mm), Minor axe ellipse (mm), Major axe Feret ellipse (mm), Video Slice Number, Minor axe Feret ellipse (mm), Volume ellipse (mm^3), Volume Feret ellipse (mm^3), Average volumes (ellipse + Feret ellipse) (mm^3), Client, Project, Service Request, Sample, Lab code, Sample type, Date collected (MM/DD/YYYY), Date received (MM/DD/YYYY), Date extracted (MM/DD/YYYY), Date analyzed (MM/DD/YYYY), Extraction method, Method, Matrix, Basis, Units, Component, Dilution factor, Reporting limit, Detection limit, Result, Result notes, Acquisition time (s), Heart beat number, Heart rate (beat/min), Ventricular volume diastolic (mm^3), Ventricular volume systolic (mm^3), Stroke volume (mm^3), Cardiac output (mm^3/min), Ventricular ejection fraction (%) Ejection vol.
                              All oil concentrations are measured in µg L-1. Water parameters (i.e. pH, ammonium, dissolved oxygen, and temperature) were measured before, during, and after oil exposure. PAH composition was measured at the beginning of the test. Test duration (hours), Date (MM/DD/YY). Oil loading rate (g/L); Control (0% oil); Treatment (% oil converted to µg L-1 after chemistry analysis); Volume (mL or L); Temperature (degrees C); Photoperiod (hours light:dark); Time (HH:MM); pH; Dissolved Oxygen (D.O., mg/L); Salinity (ppt); Total ammonia (uM); Ventricular volume (mm3); OFS = oil from surface
| All studies were conducted with 1 or 12 hours post fertilization (hpf) red drum embryos. Survival tests were carried out to 72 hpf in 1 L replicates with 20 individuals in each. Sub-lethal EC50 tests were carried out to 48 hpf in 1 L replicates with 10 individuals in each. Embryos were stored in a 26° C incubator with a 14/10 light/dark photoperiod. For survival tests, 20 embryos were placed in 1 L beakers with varying weathered/non-weathered WAF concentrations. Beakers were stored in the incubator to 72 hpf. At 24 hours, control replicates were monitored for 80% hatching success. Debris within beakers were removed daily. At 72 hpf, all survival counts were obtained. A weathered WAF was filtered through a 0.3 µm filter to remove any oil droplets suspended in the WAF, and used in a survival test to determine influence of micro-droplets on survival. For sub-lethal impacts, 10 embryos were placed in 1 L beakers with varying concentrations of weathered oil. Beakers were stored in incubator until 48 hpf. At 24 hrs, control replicates were monitored for 80% hatching success. At 48 hpf, videos and still images were collected at x4 and x8 zoom using a Nikon SMZ800N microscope. |Nikon SMZ800N microscope Metal Blender Image J|Survival tests were composed of 4 replicates per concentration with 20 individuals in each replicate. EC50 test comprised of 4 replicates per concentration with 10 individuals in each replicate. |All statistical tests were run on the biological average for each replicate to avoid psuedoreplication. LC50 and EC50 estimates and confidence intervals were calculated using the U.S. Environmental Protection Agency’s TRAP software package. In all cases, data were fit to a tolerance type Gaussian model with 3 parameters using log-transformation. The contribution of micro-droplets to survival was assessed using one-way ANOVA followed by a Holm-Sidak pairwise multiple comparisons test. Spinal curvature and craniofacial deformities were not normally distributed and therefore assessed using a one-way ANOVA run on ranks with a Kruskal-Wallis multiple comparisons test. Pericardial area was also not normally distributed and was analyzed using an ANOVA on ranks with a Dunn’s post-hoc test against the control group. Cardiac output, stroke volume and heart rate were measured using one-way ANOVA followed by a Holm-Sidak pairwise multiple comparisons test.|
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Distribution info: Distribution: Distributor: Distributor: Distributor contact: Responsible party: Organisation name: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC) Contact info: Contact: Phone: Telephone: Voice: +1-361-825-3604 Facsimile: +1-361-825-2050 Address: Address: Delivery point: 6300 Ocean Drive Unit 5869 City: Corpus Christi Administrative area: Texas Postal code: 78412-5869 Country: USA Electronic mail address: griidc@gomri.org Online Resource: Online Resource: Linkage: URL: http://data.gulfresearchinitiative.org Protocol: Role: Role code: distributor Distributor format: Format: Name: csv, xls, xlsx, mp4, png, txt, tiff Version: inapplicable File decompression technique: zip Distributor transfer options: Digital transfer options: Transfer size: 635.53 Online: Online Resource: Linkage: URL: https://data.gulfresearchinitiative.org/data/R4.x258.254:0001 Protocol:
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Metadata maintenance: Maintenance information: Maintenance and update frequency: unknown Maintenance note: This ISO metadata record was automatically generated by the GRIIDC Metadata Generator from DIF and Registry information provided to GRIIDC for dataset: R4.x258.254:0001 on 2016-10-12T14:56:10+00:00 Maintenance note: This ISO metadata record was created using the 'Check and Save to File' (with form validation) function of the GRIIDC ISO 19115-2 Metadata Editor on 2016-10-12T15:03:29+00:00
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