Effects of oil exposure on red drum (Sciaenops ocellatus) cardiac and red muscle mitochondrial function

Relationship of Effects of Cardiac Outcomes in Fish for Validation of Ecological Risk (RECOVER)

DOI:
10.7266/n7-y9mb-fn45
 
UDI:
R4.x258.000:0057
Last Update:
Mar 06 2019 00:42 UTC
 
Dataset Author(s):
Johansen, J., Esbaugh, A.
Point of Contact:
Esbaugh, Andrew
The University of Texas at Austin / Marine Science Institute
College of Natural Sciences 750 Channel View Drive
Port Aransas, Texas  78373
USA
a.esbaugh@austin.utexas.edu
Funding Source:
RFP-IV
 
Extent Description:
Dataset contains laboratory measurements of oxygen consumption, no field sampling involved.

Identified Submitted In-Review Available
3 3 3 3

Suggested Citation:

Johansen, J., Esbaugh, A.. 2019. Effects of oil exposure on red drum (Sciaenops ocellatus) cardiac and red muscle mitochondrial function. Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/n7-y9mb-fn45

Abstract:

This dataset contains raw oroboros data files, each showing the oxygen consumption traces and substrates added to the cardiac and red muscle mitochondria of red drum (Sciaenops ocellatus) after 24hrs OFS (oil from the surface) high energy water accommodated fraction (HEWAF) exposure.

Purpose:

Examine the injury of oil exposure on cardiac and red skeletal muscle in marine fish.

Theme Keywords:

red drum, young adult, cardiac, mitochondria, red muscle, skeletal muscle, oil exposure, high energy WAF (HEWAF), Sciaenops ocellatus, oxidative phosphorylation (OXPHOS), water accommodated fraction (WAF)

File Format:

dld, csv, xls, xlsx

Filename:

Red Drum Mitochondria.zip (15.34 MB)

Dataset Downloads:

5

Effects of oil exposure on red drum (Sciaenops ocellatus) cardiac and red muscle mitochondrial function



Identification Information
Distribution Information
Metadata Maintenance Information

Metadata: 
  File identifier: 
      R4.x258.000-0057-metadata.xml
  Language: 
      eng; USA
  Character set: 
    Character set code: 
      utf8
  Hierarchy level: 
    Scope code: 
      dataset
  Metadata author: 
    Responsible party: 
      Individual name: 
          Madeline Munoz-Bustamante
      Organisation name: 
          University of Miami / Rosenstiel School of Marine and Atmospheric Science
      Position name: 
          Program Manager
      Contact info: 
        Contact: 
          Phone: 
            Telephone: 
              Voice: 
              Facsimile: 
          Address: 
            Address: 
              Delivery point: 
                  4600 Rickenbacker Cswy
              City: 
                  Miami
              Administrative area: 
                  Florida
              Postal code: 
                  33149
              Country: 
                  USA
              Electronic mail address: 
                  mbustamante@rsmas.miami.edu
      Role: 
        Role code: 
          pointOfContact
  Date stamp: 
      2021-05-04T20:04:57+00:00
  Metadata standard name: 
      ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
  Metadata standard version: 
      ISO 19115-2:2009(E)
  Dataset URI: 
      https://data.gulfresearchinitiative.org/metadata/R4.x258.000:0057
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Identification info: Data identification: Citation: Citation: Title: Effects of oil exposure on red drum (Sciaenops ocellatus) cardiac and red muscle mitochondrial function Alternate title: Date: Date: Date: 2019-05-02 Date type: Date type code: publication Identifier: Identifier: Code: Anchor: xlink: https://dx.doi.org/10.7266/n7-y9mb-fn45 title: DOI doi:10.7266/n7-y9mb-fn45 Abstract: This dataset contains raw oroboros data files, each showing the oxygen consumption traces and substrates added to the cardiac and red muscle mitochondria of red drum (Sciaenops ocellatus) after 24hrs OFS (oil from the surface) high energy water accommodated fraction (HEWAF) exposure. Purpose: Examine the injury of oil exposure on cardiac and red skeletal muscle in marine fish. Status: Progress code: completed Point of contact: Responsible party: Individual name: Andrew Esbaugh Organisation name: The University of Texas at Austin / Marine Science Institute Position name: Assistant Professor Contact info: Contact: Phone: Telephone: Voice: 3617496835 Facsimile: Address: Address: Delivery point: College of Natural Sciences 750 Channel View Drive City: Port Aransas Administrative area: Texas Postal code: 78373 Country: USA Electronic mail address: a.esbaugh@austin.utexas.edu Role: Role code: pointOfContact Point of contact: Responsible party: Individual name: Jacob Johansen Organisation name: The University of Texas at Austin / Department of Marine Science Position name: Postdoctoral Fellow Contact info: Contact: Phone: Telephone: Voice: Facsimile: Address: Address: Delivery point: Marine Science Institute 750 Channel View Drive City: Port Aransas Administrative area: Texas Postal code: 78373 Country: USA Electronic mail address: jacob.johansen@utexas.edu Role: Role code: pointOfContact Descriptive keywords: Keywords: Keyword: red drum Keyword: young adult Keyword: cardiac Keyword: mitochondria Keyword: red muscle Keyword: skeletal muscle Keyword: oil exposure Keyword: high energy WAF (HEWAF) Keyword: Sciaenops ocellatus Keyword: oxidative phosphorylation (OXPHOS) Keyword: water accommodated fraction (WAF) Type: Keyword type code: theme Descriptive keywords: Keywords: Keyword: inapplicable Type: Keyword type code: place Resource constraints: title: Cite As Constraints: Use limitation: Johansen, J., Esbaugh, A.. 2019. Effects of oil exposure on red drum (Sciaenops ocellatus) cardiac and red muscle mitochondrial function. Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/n7-y9mb-fn45 Resource constraints: title: CC0 License Legal constraints: Use constraints: Restriction code: licenceUnrestricted Other constraints: This information is released under the Creative Commons license - No Rights Reserved - CC0 1.0 Universal (https://creativecommons.org/publicdomain/zero/1.0/). The person who associated a work with this deed has dedicated the work to the public domain by waiving all of his or her rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law. You can copy, modify, distribute and perform the work, even for commercial purposes, all without asking permission. Resource constraints: title: Liability and Warranty Legal constraints: Other constraints: All materials on this website are made available to GRIIDC and in turn to you "as-is." Content may only be submitted by an individual who represents and warrants that s/he has sufficient rights to be able to make the content available under a CC0 waiver. There is no warranty (expressed or implied) to these materials, their title, accuracy, non-infringement of third party rights, or fitness for any particular purpose, including the performance or results you may obtain from their use. Use these materials at your own risk. Under no circumstances shall GRIIDC be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use or the inability to use either this website or the materials available via this website. If you are dissatisfied with any website feature, content, or terms of use, your sole and exclusive remedy is to discontinue use. Aggregation Info: AggregateInformation: Aggregate Data Set Name: title: Related Publication Citation Citation: Title: Johansen, J. L., & Esbaugh, A. J. (2019). Oil-induced responses of cardiac and red muscle mitochondria in red drum (Sciaenops ocellatus). Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 219, 35–41. doi:10.1016/j.cbpc.2019.02.003 Date: inapplicable Aggregate Data Set Identifier: title: Related Publication DOI Identifier: Code: Anchor: xlink: https://dx.doi.org/10.1016/j.cbpc.2019.02.003 title: DOI doi:10.1016/j.cbpc.2019.02.003 Association Type: Association type code: crossReference Language: eng; USA Topic category: Topic category code: biota Extent: Extent: Description: Dataset contains laboratory measurements of oxygen consumption, no field sampling involved. Supplemental Information: The folder "Raw oroboros data": Contains raw oroboros data files in DLD and CSV file formats, each showing the oxygen consumption traces and substrates added to the cardiac and red muscle mitochondria of red drum (Sciaenops ocellatus) after 24hrs OFS (oil from the surface) high energy water accommodated fraction (HEWAF) exposure. File naming convention for all the files is 'Redfish (oil concentration) (ID) (date)".
          
The file “Mitochondria redfish –FINAL –Cont ALL DATA –Dec 2017.xlsx": Contains multiple worksheets; the worksheet "Mitochondria" includes- Sample ID, Exposure, Date, Time, pH, Temp, Sal, DO, Date, Time, pH, Temp, Sal, DO, Ammonia, Weight, TL, SL, Heart (mg), Muscle (mg), Cytochrome activity, Success?, Oil weight, Time start, Time in cone, Time out of cone, % HEWAF, Analyte sample (0 Hr, 24 Hr), 0Hr Oil name, and 24HR Oil name. The worksheets “Cardiac Control”, “Red Control”, “Cardiac 20%HEWAF”, ”RED 20%HEWAF”, “Cardiac 20%HEWAF” and “Red 40%HEWAF” includes - Complex I, Cyt C (Cytochrome C), OXPHOS (oxidative phosphorylation), OXPHOS with ADP (oxidative phosphorylation with adenosine diphosphate), LEAK, Max uncoupled ETS (Electron transport system), Inhibit Complex I, ROX (residual oxygen consumption), O2 (pmol/(s*mg)), mean, SD (standard deviation), n, and SE (Standard error), C1-C60 (Various tanks).
          
The file TCT E039 -Mitochondria.xls: Contains multiple worksheets; the worksheet "Test condition table" includes - test condition parameters and values. The worksheet "Tank ID Dilution or Stock Code” includes - Tank ID Dilution or Stock Code, Start date, Start time, End date, End time, Nominal Treatment Concentration, Treatment Units, Replicate number, Notes, and Recorded by information. The worksheet "WAF Preparation” includes WAF Prep 1, WAF Prep 2, WAF Prep 3, …. WAF Prep 45. The worksheet "Water Quality Monitoring" includes - Tank ID Dilution or Stock Code, Date, Time, Period, Water temp. (C), "pH (S.U.)", D.O. (mg/L), Salinity (ppt), and Total ammonia (µM). The worksheet "Sample inventory" includes - Sampling date, Sampling time, Tank ID Dilution or Stock Code, Number organisms per sample, Unique ID Number, Sample ID, Description of Sample, Storage location, and Notes Recorded by information.
          
Protocol = tissue examined (cardiac muscle or skeletal muscle); Sample type = Chamber used in the respirometer (chamber A or B); Cohort = tissue examined (cardiac muscle or skeletal muscle); Sample code = Fish ID; Sample concentration = amount of tissue per ml; Chamber volume = 2ml; Sample amount = amount of tissue in total; R1 = Voltage reading of oxygen saturated sample (control value); R0 = Voltage reading of oxygen-depleted sample (control value); Pb = partial pressure of oxygen in the atmosphere, measured in kPa; FM = calibration factor; O2 background a° = slope of oxygen depletion in the chamber at the beginning of the trial O2 background b° = slope of oxygen depletion in the chamber at the end of the trial; Start = time of start for measuring each parameter during the trial; Stop = time of end for measuring each parameter during the trial; N points = number of individual oxygen measurements completed during the trial period for each parameter. Parameters - Start = Start-Complex1 -leak state = background respiration of tissues within the chamber without addition of mitochondrial substrates; Complex 1 = ADP-Complex1-Phos state = tissue respiration after addition of catalase, hydrogen peroxide, malate, pyruvate, glutamate and ADP; Cyt c = Cyt C-Membrane integrity = tissue respiration after addition of cytochrome C; OXPHOS = Succ-Complex 1&2-Phos ste P = tissue respiration after addition of succinate; OXPOS with ADP = ADP-Complex1&2-Phos ste P = tissue respiration after addition of ADO; LEAK = Omy-LEAK L = tissue respiration after addition of oligomycin; Max uncoupled ETS = FCCP-Max uncoupled ETS E = tissue respiration after addition of FCCP; Inhibit Complex I = Rot -Complex1 Inhibit -Com2e = tissue respiration after addition of rotenone; ROX = AntiA-Complex2 inhibit ROX = tissue respiration after addition of antimycin A; Control ratios = subtraction or division between the parameters E (max uncoupled ETS), P (OXPHOS), L (Leak), ROX, cytochrome C change (Cyt c).
          
Definition of all the abbreviation and other notes: HEWAF = high energy water accommodated fractions; PAH = Polycyclic Aromatic Hydrocarbons; Temperature = Centigrades = 22.5C; Time/recovery = 24hr; Exposure/Conc = 0% (0HEWAF), 20% (20HEWAF), 40% (40HEWAF) HEWAF = 0, 29.6ug/L or 64.5ug/L PAH; Species: Sciaenops ocellatus; Weight = mgram; Standard body length = Standard length in cm (SL); Total body length = total body length of fish in cm (TL); Tank and stock ID: C = control, T = oil exposure, (example T12); D.O.=Dissolved Oxygen; Sal = salinity of water in ppt; pH = pH of water; Tissue: Heart = cardiac mitochondria, Muscle = red skeletal muscle mitochondria;se = standard error; ADP = Adenosine diphosphate; ATP = Adenosine triphosphate ; Cyt c = cytochrome c; Succ=succinate; OMY = Oligomycin; FCCP = Carbonyl cyanide-4 (trifluoromethoxy)phenylhydrazone; Rot = Rotenone; Anti A = Antimycin A; Mal = Malate; Pyr = Pyruvate; Glu = Glutamate; h202 = Hydrogen peroxide.|Mitochondrial function was assessed using an Oroboros Oxygraph 2k™ respirometer system (Oroboros Instruments, Innsbruck, Austria). Two Oroboros chambers were prepared with 2.0 ml MiR05 solution at 22.0 °C. The permeabilized cardiac and red skeletal tissues of a single individual were carefully blotted on an absorbent tissue and weighed on a 0.1 mg precision scale. A total of 5.4 ± 0.3 mg cardiac and 12.5 ± 0.5 mg red skeletal tissue (mean ± S.E.M.) were then placed into the respective test chambers. Respiration was measured as weight/specific O2 flux (pmol s/1 mg/1), calculated in real/time using the negative time derivative of O2 concentration and recorded in DatLab (Oroboros Instruments). Mitochondrial function and efficiency were assessed using the following substrate and solution injection sequences: a) 5uL catalase stock solution and 3.0 uL H2O2; b) 5.0 uL of 0.8 M malate; c) 5.0 uL of 2.0 M pyruvate; d) 10.0 uL of 2.0 M glutamate. These initial injections provided the fibers with complex I substrate in the absence of ADP, thereby allowing measurement of steady/state basal respiration in a non-phosphorylating leak state (CI-Leak). Complex I oxidative phosphorylation (CI-OXPHOS) was stimulated by adding 0.5 M ADP in 5uL steps until saturation. Cytochrome C (5 uL of 4 M stock) was then added to assess the integrity of the mitochondrial membrane. In cases where OXPHOS increased more than 10 %, the membrane integrity was deemed compromised and the protocol was terminated. Maximal respiration flux (OXPHOS) was assessed by adding Complex II substrate (20.0 uL of 1.0 M succinate) in addition to 5.0 uL of 0.5 M ADP to verify saturation. Respiration rate owing to the passive leak of protons across the mitochondrial membrane (LEAK) was assessed by injection of the ATP synthase inhibitor oligomycin (1.0 uL of 4.0 mg/ml stock). Uncoupled electron transport capacity (ETS) was assessed by adding the protonophore FCCP (1 M stock) in 0.5 uL steps until saturation. Contributions of complex II to ETS was assessed by inhibition of complex I via injection of 1.0 uL of 1.0 M rotenone. Finally, to control for other oxygen-consuming processes, remaining flux from the ETS (i.e. complex II and III) were inhibited via injection of 1.0 uL of 5.0 M Antimycin A (leaving only residual oxygen consumption, ROX).|Oroboros Oxygraph 2k™ respirometer system (Oroboros Instruments, Innsbruck, Austria).|||
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Distribution info: Distribution: Distributor: Distributor: Distributor contact: Responsible party: Organisation name: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC) Contact info: Contact: Phone: Telephone: Voice: 3618253604 Address: Address: Delivery point: 6300 Ocean Drive City: Corpus Christi Administrative area: TX Postal code: 78412 Country: USA Electronic mail address: griidc@gomri.org Online Resource: Online Resource: Linkage: URL: https://data.gulfresearchinitiative.org Role: Role code: distributor Distributor format: Format: Name: dld, csv, xls, xlsx Version: inapplicable File decompression technique: zip Distributor transfer options: Digital transfer options: Transfer size: 15.3381 Online: Online Resource: Linkage: URL: https://data.gulfresearchinitiative.org/data/R4.x258.000:0057 Protocol: https Name: Data Landing Page Description: GRIIDC dataset landing page Function: Online function code: information
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Metadata maintenance: Maintenance information: Maintenance and update frequency: unknown Maintenance note: This ISO metadata record was automatically generated from information provided to GRIIDC for dataset: R4.x258.000:0057 on 2021-07-29T02:48:46-05:00
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