Metadata: 
  File identifier: 
      R4.x258.000-0040-metadata.xml
  Language: 
      eng; USA
  Character set: 
    Character set code: 
      utf8
  Hierarchy level: 
    Scope code: 
      dataset
  Metadata author: 
    Responsible party: 
      Individual name: 
          Madeline Munoz-Bustamante
      Organisation name: 
          University of Miami / Rosenstiel School of Marine and Atmospheric Science
      Position name: 
          Program Manager
      Contact info: 
        Contact: 
          Phone: 
            Telephone: 
              Voice: 
              Facsimile: 
          Address: 
            Address: 
              Delivery point: 
                  4600 Rickenbacker Cswy
              City: 
                  Miami
              Administrative area: 
                  Florida
              Postal code: 
                  33149
              Country: 
                  USA
              Electronic mail address: 
                  mbustamante@rsmas.miami.edu
      Role: 
        Role code: 
          pointOfContact
  Date stamp: 
      2018-08-11T13:43:09+00:00
  Metadata standard name: 
      ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
  Metadata standard version: 
      ISO 19115-2:2009(E)
  Dataset URI: 
      https://data.gulfresearchinitiative.org/metadata/R4.x258.000:0040
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  Identification info: 
    Data identification: 
      Citation: 
        Citation: 
          Title: 
              MicroRNA sequencing data from Mahi exposed samples
          Alternate title: 
          Date: 
            Date: 
              Date: 
                  2017-09-06
              Date type: 
                Date type code: 
                  creation
      Abstract: 
          MicroRNAs (miRNA) play key roles in diverse biological processes including heart development. In our study, we 
          evaluated the effects of DHW oil on miRNA expression in mahi-mahi (Coryphaena hippurus) embryos exposed to 
          WAF from weathered slick oil and non-weathered source oil. miRNA and mRNA was compared using downstream 
          bioinformatics with subsequent target organ predictions based on their interactions. Using two methods miRNA 
          sequences were annotated using the Fugu rubripes genome or using BLAST. Our data showed that exposure of 
          embryos to slick oil resulted in more differentially expressed (DE) miRNAs than source oil at all 
          developmental stages 24hpf, 48hpf and 96hpf. There was also an increase in the number of DE miRNA as 
          development progressed, 96h having the highest number of DE miRNAs. Gene ontology (GO) analysis on the 
          target mRNAs was consistent with pathways analysis of miRNAs, predicting disruption of cardiovascular system 
          development after oil exposure and showed specific miRNA–mRNA interactions that may contribute to these 
          effects. This work is the first study linking miRNAs and mRNAs in fish responsive to DWH oil exposure, 
          providing a new opportunity for better understanding of the molecular mechanism of DWH oil toxicity.
      Purpose: 
          To further understand the role of microRNAs on oil induced toxicity at different time points during development.
      Status: 
        Progress code: 
          completed
      Point of contact: 
        Responsible party: 
          Individual name: 
              Daniel Schlenk
          Organisation name: 
              University of California Riverside / Department of Environmental Sciences
          Position name: 
              Professor
          Contact info: 
            Contact: 
              Phone: 
                Telephone: 
                  Voice: 
                      9518272018
                  Facsimile: 
              Address: 
                Address: 
                  Delivery point: 
                      900 University Avenue
                      Geology 2258
                  City: 
                      Riverside
                  Administrative area: 
                      California
                  Postal code: 
                      92521
                  Country: 
                      USA
                  Electronic mail address: 
                      daniel.schlenk@ucr.edu
          Role: 
            Role code: 
              pointOfContact
      Descriptive keywords: 
        Keywords: 
          Keyword: 
              mahi mahi
          Keyword: 
              microRNA
          Keyword: 
              Slick Oil
          Keyword: 
              Source Oil
          Keyword: 
              Coryphaena hippurus
          Type: 
            Keyword type code: 
              theme
      Descriptive keywords: 
        Keywords: 
          Keyword: 
            inapplicable
          Type: 
            Keyword type code: 
              place
      Language: 
          eng; USA
      Topic category: 
        Topic category code: 
          biota
      Extent: 
        Extent: 
          Description: 
              Dataset contains micro RNA sequencing, no field sampling involved.
      Supplemental Information: 
          Number (NCBI GEO Accession number), File name, Stage (hpf), Concentration (g/L), Oil type (Control, Slick, or 
          Source) microRNA expression analysis Data are available at NCBI Accession Number GSE102966, Bioproject 
          Accession PRJNA399845 and SRA Study SRP116083|microRNA sequencing using NEBNext® Small RNA Library Prep Set 
          for Illumina|NEXTseq v2|||
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  Distribution info: 
    Distribution: 
      Distributor: 
        Distributor: 
          Distributor contact: 
            Responsible party: 
              Organisation name: 
                  Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC)
              Contact info: 
                Contact: 
                  Phone: 
                    Telephone: 
                      Voice: 
                          3618253604
                  Address: 
                    Address: 
                      Delivery point: 
                          6300 Ocean Drive
                      City: 
                          Corpus Christi
                      Administrative area: 
                          TX
                      Postal code: 
                          78412
                      Country: 
                          USA
                      Electronic mail address: 
                          griidc@gomri.org
                  Online Resource: 
                    Online Resource: 
                      Linkage: 
                        URL: 
                          https://data.gulfresearchinitiative.org
              Role: 
                Role code: 
                  distributor
          Distributor format: 
            Format: 
              Name: 
                  xlsx
              Version: 
                inapplicable
              File decompression technique: 
          Distributor transfer options: 
            Digital transfer options: 
              Transfer size: 
                  0.0531
              Online: 
                Online Resource: 
                  Linkage: 
                    URL: 
                      https://data.gulfresearchinitiative.org/data/R4.x258.000:0040
                  Protocol: 
                      https
                  Name: 
                      Data Landing Page
                  Description: 
                      GRIIDC dataset landing page
                  Function: 
                    Online function code: 
                      information
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  Metadata maintenance: 
    Maintenance information: 
      Maintenance and update frequency: 
        unknown
      Maintenance note: 
          This ISO metadata record was automatically generated from information provided to GRIIDC for dataset: 
          R4.x258.000:0040 on 2019-09-22T16:10:43-05:00
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