Abstract:

MicroRNAs (miRNA) play key roles in diverse biological processes including heart development. In our study, we evaluated the effects of DHW oil on miRNA expression in mahi-mahi (Coryphaena hippurus) embryos exposed to WAF from weathered slick oil and non-weathered source oil. miRNA and mRNA was compared using downstream bioinformatics with subsequent target organ predictions based on their interactions. Using two methods miRNA sequences were annotated using the Fugu rubripes genome or using BLAST. Our data showed that exposure of embryos to slick oil resulted in more differentially expressed (DE) miRNAs than source oil at all developmental stages 24hpf, 48hpf and 96hpf. There was also an increase in the number of DE miRNA as development progressed, 96h having the highest number of DE miRNAs. Gene ontology (GO) analysis on the target mRNAs was consistent with pathways analysis of miRNAs, predicting disruption of cardiovascular system development after oil exposure and showed specific miRNA–mRNA interactions that may contribute to these effects. This work is the first study linking miRNAs and mRNAs in fish responsive to DWH oil exposure, providing a new opportunity for better understanding of the molecular mechanism of DWH oil toxicity.

Suggested Citation:

Schlenk, Daniel; Diamante, Graciel. 2017. MicroRNA sequencing data from Mahi exposed samples. Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N70Z71QJ

Publications:

Diamante, G., Xu, E. G., Chen, S., Mager, E., Grosell, M., & Schlenk, D. (2017). Differential Expression of MicroRNAs in Embryos and Larvae of Mahi-Mahi (Coryphaena hippurus) Exposed to Deepwater Horizon Oil. Environmental Science & Technology Letters, 4(12), 523–529. doi:10.1021/acs.estlett.7b00484

Purpose:

To further understand the role of microRNAs on oil induced toxicity at different time points during development.

Data Parameters and Units:

Number (NCBI GEO Accession number), File name, Stage (hpf), Concentration (g/L), Oil type (Control, Slick, or Source) microRNA expression analysis Data are available at NCBI Accession Number GSE102966, Bioproject Accession PRJNA399845 and SRA Study SRP116083

Methods:

microRNA sequencing using NEBNext® Small RNA Library Prep Set for Illumina

Instruments:

NEXTseq v2

Individual Files: