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Combined effects of oil exposure, temperature and UV-radiation on buoyancy and oxygen consumption of embryonic mahi-mahi, Coryphaena hippurus

Relationship of Effects of Cardiac Outcomes in Fish for Validation of Ecological Risk (RECOVER)

DOI:
10.7266/N7GH9G95
 
UDI:
R4.x258.000:0010
Last Update:
Oct 31 2017 20:24 UTC
 
Dataset Author(s):
Sweet, Lauren; Pasparakis, Christina; Roberts, Aaron P.
Point of Contact:
Roberts, Aaron
University of North Texas / Department of Biological Sciences
1511 West Sycamore Street Life Sciences Complex
Denton, Texas  76203
USA
Aaron.Roberts@unt.edu
Funding Source:
RFP-IV
 
Extent Description:
Dataset contains laboratory measurements of oxygen consumption, no field sampling involved.

Identified Submitted Review Available
3 3 3 3

Abstract:

The timing and location of the 2010 Deepwater Horizon (DWH) incident within the Gulf of Mexico pelagic zone likely resulted in exposure of commercially and ecologically important fish species, such as mahi-mahi (Coryphaena hippurus) during the sensitive early life stages. A 24-channel optical fluorescence oxygen sensing system for high through-put respiration measurements was used to investigate the individual and combined effects of oil exposure, temperature and UV-exposure on oxygen consumption rates in embryonic mahi-mahi. Images included in the dataset were used to calculate yolk sac depletion by calculating the number of pixels in both the yolk sac and chorion area of embryos. Raw data on embryo buoyancy (measured on a range of -1 to +1) and hatch rate data are also provided.

Purpose:

Testing combined effects of oil exposure, different rearing temperatures and UV radiation on oxygen consumption, drop rate, buoyancy, hatch rate and energy utilization in embryonic mahi-mahi.

Theme Keywords:

buoyancy, oil spill, fish development, toxicity, multi stressors, metabolic rate, UV radiation, temperature, embryo, mahi-mahi, Coryphaena hippurus

File Format:

csv, xls, jpg, png

Dataset Downloads:

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Combined effects of oil exposure, temperature and UV-radiation on buoyancy and oxygen consumption of embryonic mahi-mahi, Coryphaena hippurus



Identification Information
Distribution Information
Metadata Maintenance Information

Metadata: 
  File identifier: 
      R4.x258.000-0010-metadata.xml
  Language: 
      eng; USA
  Character set: 
    Character set code: 
      utf8
  Hierarchy level: 
    Scope code: 
      dataset
  Metadata author: 
    Responsible party: 
      Individual name: 
          Aaron Roberts
      Organisation name: 
          University of North Texas / Department of Biological Sciences
      Position name: 
          Assistant Professor
      Contact info: 
        Contact: 
          Phone: 
            Telephone: 
              Voice: 
                  9408916957
              Facsimile: 
          Address: 
            Address: 
              Delivery point: 
                  1511 West Sycamore Street
                  Life Sciences Complex
              City: 
                  Denton
              Administrative area: 
                  Texas
              Postal code: 
                  76203
              Country: 
                  USA
              Electronic mail address: 
                  Aaron.Roberts@unt.edu
      Role: 
        Role code: 
          pointOfContact
  Date stamp: 
      2018-08-11T13:38:49+00:00
  Metadata standard name: 
      ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
  Metadata standard version: 
      ISO 19115-2:2009(E)
  Dataset URI: 
      https://data.gulfresearchinitiative.org/metadata/R4.x258.000:0010
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Identification info: Data identification: Citation: Citation: Title: Combined effects of oil exposure, temperature and UV-radiation on buoyancy and oxygen consumption of embryonic mahi-mahi, Coryphaena hippurus Alternate title: Date: Date: Date: 2017-03-23 Date type: Date type code: creation Abstract: The timing and location of the 2010 Deepwater Horizon (DWH) incident within the Gulf of Mexico pelagic zone likely resulted in exposure of commercially and ecologically important fish species, such as mahi-mahi (Coryphaena hippurus) during the sensitive early life stages. A 24-channel optical fluorescence oxygen sensing system for high through-put respiration measurements was used to investigate the individual and combined effects of oil exposure, temperature and UV-exposure on oxygen consumption rates in embryonic mahi-mahi. Images included in the dataset were used to calculate yolk sac depletion by calculating the number of pixels in both the yolk sac and chorion area of embryos. Raw data on embryo buoyancy (measured on a range of -1 to +1) and hatch rate data are also provided. Purpose: Testing combined effects of oil exposure, different rearing temperatures and UV radiation on oxygen consumption, drop rate, buoyancy, hatch rate and energy utilization in embryonic mahi-mahi. Status: Progress code: completed Point of contact: Responsible party: Individual name: Aaron Roberts Organisation name: University of North Texas / Department of Biological Sciences Position name: Assistant Professor Contact info: Contact: Phone: Telephone: Voice: 9408916957 Facsimile: Address: Address: Delivery point: 1511 West Sycamore Street Life Sciences Complex City: Denton Administrative area: Texas Postal code: 76203 Country: USA Electronic mail address: Aaron.Roberts@unt.edu Role: Role code: pointOfContact Descriptive keywords: Keywords: Keyword: buoyancy Keyword: oil spill Keyword: fish development Keyword: toxicity Keyword: multi stressors Keyword: metabolic rate Keyword: UV radiation Keyword: temperature Keyword: embryo Keyword: mahi-mahi Keyword: Coryphaena hippurus Type: Keyword type code: theme Descriptive keywords: Keywords: Keyword: inapplicable Type: Keyword type code: place Language: eng; USA Topic category: Topic category code: biota Extent: Extent: Description: Dataset contains laboratory measurements of oxygen consumption, no field sampling involved. Supplemental Information: Oxygen Consumption (% air saturation and umol L-1)-data found in ‘Microplate Raw Data’ folder within additional folders labeled by the different treatment conditions (temperature oC and salinity trials), microplate number (plate 368 or 538) and trial conducted (Trials 1-4). Data is presented as either % air saturation or umol L-1 by elapsed time (seconds). Date (DD.MM.YYYY HH:MM:SS), Time (seconds). Drop rate (cm/sec)-data found in “Drop Rate” folder for trials conducted at both temperature treatments. Images taken to measure yolk sac depletion- data found in “Yolk Sac Depletion” folder within additional folder labeled by temperature treatment and exposure treatment. Buoyancy (scale of -1 (complete negative buoyancy) to +1 (complete positive buoyancy) and hatch rate (%)- data found in “Buoyancy and Hatch Rate” folder within additional folders labeled by different temperature trials. Sum PAH data- found in folder titled ‘Sum PAH data’. Raw data provided along with break down and percentages of different ringed PAHs. The second tab of the excel files has compiled data and geometric means of initial and final Sum PAH samples.|Surface slick oil from the DWH spill was prepared as a high-energy-water-accommodated fraction (HEWAF) to generate environmentally relevant PAH concentrations and compositional profiles. Each HEWAF was prepared on the day of use at a loading rate of 1g of oil per liter of 1 μm filtered, UV-sterilized seawater and mixed in a Waring CB15 blender (Torrington, CT) at low speed for 30 s. The mixture was immediately transferred to a glass separatory funnel and left to settle for 1 hr. The lower 90% of the mixture was then carefully drained and retained for subsequent use as 100% WAF (unfiltered) that was diluted to 4% using UV-sterilized seawater for test exposures. 4% HEWAF dilutions were used for treatment exposures (Oil only and UV + Oil). UV-sterilized seawater was used for control treatments (Control and UV Only). Loligo microplate was used to measure oxygen consumption in developing mahi-mahi. Yolk sac and embryo area were measured using ImageJ version 1.46r (rsbweb.nih.gov/ij/) from a perimeter drawn with the freehand tool enclosing the area.|Oxygen Consumption- Loligo Microplate (24 well optical-fluorescence, oxygen-sensing system). Images and videos were taken using either a Fire-i400 or Fire-i530c digital camera (Unibrain, San Ramon, CA) mounted on a Nikon SMZ800 stereomicroscope. UVA light bulbs were purchased from HTG Supply (Callery, PA, USA). Irradiance (λ= 380 nm) was measured continuously throughout the UV co-exposures using BioSpherical radiometers (BioSpherical Instruments, Inc., San Diego, CA, USA).|Approximately 10-15 embryos were placed in 250 mL Pyrex crystallizing dishes. Four trials of oxygen consumption and drop rate were conducted on day 2. Water quality was taken daily. Oil samples were taken immediately before and after 22-hr exposure periods for most trials. In some trials only initial only samples were taken.||
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Distribution info: Distribution: Distributor: Distributor: Distributor contact: Responsible party: Organisation name: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC) Contact info: Contact: Phone: Telephone: Voice: 3618253604 Address: Address: Delivery point: 6300 Ocean Drive City: Corpus Christi Administrative area: TX Postal code: 78412 Country: USA Electronic mail address: griidc@gomri.org Online Resource: Online Resource: Linkage: URL: https://data.gulfresearchinitiative.org Role: Role code: distributor Distributor format: Format: Name: csv, xls, jpg, png Version: inapplicable File decompression technique: zip Distributor transfer options: Digital transfer options: Transfer size: 195.4463 Online: Online Resource: Linkage: URL: https://data.gulfresearchinitiative.org/data/R4.x258.000:0010 Protocol: https
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Metadata maintenance: Maintenance information: Maintenance and update frequency: unknown Maintenance note: This ISO metadata record was automatically generated from information provided to GRIIDC for dataset: R4.x258.000:0010 on 2018-08-18T10:44:12+00:00
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