Effects of oil on two species of fiddler crabs (Uca longisignalis and Uca panacea)

The Effect of Sediment Bioturbators on the Biological Degradation of Petroleum in Coastal Ecosystems

DOI:
10.7266/N76Q1VNR
 
UDI:
R2.x226.000:0008
Last Update:
Sep 07 2017 19:38 UTC
 
Dataset Author(s):
Franco, M., Klerks, P.
Point of Contact:
Klerks, Paul L.
University of Louisiana at Lafayette / Department of Biology
P.O.Box 42451
Lafayette, Louisiana  70504
USA
klerks@louisiana.edu
Funding Source:
RFP-II
 
Extent Description:
Dataset contains results from laboratory experiments using sediment and organisms collected from a site not impacted by oil. Fiddler crabs were collected at the coastal marshes and sand banks of Elmer’s Island and Grand Isle State Park in Grand Isle, Louisiana.

Identified Submitted In-Review Available
3 3 3 3

Suggested Citation:

Franco, M., Klerks, P.. 2017. Effects of oil on two species of fiddler crabs (Uca longisignalis and Uca panacea). Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N76Q1VNR

Abstract:

This dataset includes results from laboratory experiments with oil, sediment and fiddler crabs of the genus Uca, to assess whether their burrowing (and potential for bioturbation) is affected by exposure to oil. Experiments are focusing on survival and burrowing activity for two different species of fiddler crab.

Purpose:

To assess whether oil in the intertidal has an effect on fiddler crabs and their burrowing activity

Theme Keywords:

fiddler crab, burrowing, oxidative stress, oil, toxicity, bioturbation, Uca longisignalis, Uca panacea

File Format:

xlsx

Filename:

Fiddler_crab_burrowing_oil_stress.zip (69.26 KB)

Dataset Downloads:

4

Effects of oil on two species of fiddler crabs (Uca longisignalis and Uca panacea)



Identification Information
Distribution Information
Metadata Maintenance Information

Metadata: 
  File identifier: 
      R2.x226.000-0008-metadata.xml
  Language: 
      eng; USA
  Character set: 
    Character set code: 
      utf8
  Hierarchy level: 
    Scope code: 
      dataset
  Metadata author: 
    Responsible party: 
      Individual name: 
          Paul L. Klerks
      Organisation name: 
          University of Louisiana at Lafayette / Department of Biology
      Position name: 
          Professor
      Contact info: 
        Contact: 
          Phone: 
            Telephone: 
              Voice: 
                  3374826356
              Facsimile: 
          Address: 
            Address: 
              Delivery point: 
                  P.O.Box 42451
              City: 
                  Lafayette
              Administrative area: 
                  Louisiana
              Postal code: 
                  70504
              Country: 
                  USA
              Electronic mail address: 
                  klerks@louisiana.edu
      Role: 
        Role code: 
          pointOfContact
  Date stamp: 
      2021-05-04T20:04:56+00:00
  Metadata standard name: 
      ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
  Metadata standard version: 
      ISO 19115-2:2009(E)
  Dataset URI: 
      https://data.gulfresearchinitiative.org/metadata/R2.x226.000:0008
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Identification info: Data identification: Citation: Citation: Title: Effects of oil on two species of fiddler crabs (Uca longisignalis and Uca panacea) Alternate title: Effects of oil on two species of fiddler crabs (lipid peroxidation, burrowing) Date: Date: Date: 2017-09-07 Date type: Date type code: publication Identifier: Identifier: Code: Anchor: xlink: https://dx.doi.org/10.7266/N76Q1VNR title: DOI doi:10.7266/N76Q1VNR Abstract: This dataset includes results from laboratory experiments with oil, sediment and fiddler crabs of the genus Uca, to assess whether their burrowing (and potential for bioturbation) is affected by exposure to oil. Experiments are focusing on survival and burrowing activity for two different species of fiddler crab. Purpose: To assess whether oil in the intertidal has an effect on fiddler crabs and their burrowing activity Status: Progress code: completed Point of contact: Responsible party: Individual name: Paul L. Klerks Organisation name: University of Louisiana at Lafayette / Department of Biology Position name: Professor Contact info: Contact: Phone: Telephone: Voice: 3374826356 Facsimile: Address: Address: Delivery point: P.O.Box 42451 City: Lafayette Administrative area: Louisiana Postal code: 70504 Country: USA Electronic mail address: klerks@louisiana.edu Role: Role code: pointOfContact Descriptive keywords: Keywords: Keyword: fiddler crab Keyword: burrowing Keyword: oxidative stress Keyword: oil Keyword: toxicity Keyword: bioturbation Keyword: Uca longisignalis Keyword: Uca panacea Type: Keyword type code: theme Descriptive keywords: Keywords: Keyword: inapplicable Type: Keyword type code: place Resource constraints: title: Cite As Constraints: Use limitation: Franco, M., Klerks, P.. 2017. Effects of oil on two species of fiddler crabs (Uca longisignalis and Uca panacea). Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N76Q1VNR Resource constraints: title: CC0 License Legal constraints: Use constraints: Restriction code: licenceUnrestricted Other constraints: This information is released under the Creative Commons license - No Rights Reserved - CC0 1.0 Universal (https://creativecommons.org/publicdomain/zero/1.0/). The person who associated a work with this deed has dedicated the work to the public domain by waiving all of his or her rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law. You can copy, modify, distribute and perform the work, even for commercial purposes, all without asking permission. Resource constraints: title: Liability and Warranty Legal constraints: Other constraints: All materials on this website are made available to GRIIDC and in turn to you "as-is." Content may only be submitted by an individual who represents and warrants that s/he has sufficient rights to be able to make the content available under a CC0 waiver. There is no warranty (expressed or implied) to these materials, their title, accuracy, non-infringement of third party rights, or fitness for any particular purpose, including the performance or results you may obtain from their use. Use these materials at your own risk. Under no circumstances shall GRIIDC be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use or the inability to use either this website or the materials available via this website. If you are dissatisfied with any website feature, content, or terms of use, your sole and exclusive remedy is to discontinue use. Aggregation Info: AggregateInformation: Aggregate Data Set Name: title: Related Publication Citation Citation: Title: Franco, M. E., Felgenhauer, B. E., & Klerks, P. L. (2017). Crude oil toxicity to fiddler crabs (Uca longisignalis and Uca panacea ) from the northern Gulf of Mexico: Impacts on bioturbation, oxidative stress, and histology of the hepatopancreas. Environmental Toxicology and Chemistry. doi:10.1002/etc.3982 Date: inapplicable Aggregate Data Set Identifier: title: Related Publication DOI Identifier: Code: Anchor: xlink: https://dx.doi.org/10.1002/etc.3982 title: DOI doi:10.1002/etc.3982 Association Type: Association type code: crossReference Language: eng; USA Topic category: Topic category code: biota Topic category: Topic category code: environment Extent: Extent: Description: Dataset contains results from laboratory experiments using sediment and organisms collected from a site not impacted by oil. Fiddler crabs were collected at the coastal marshes and sand banks of Elmer’s Island and Grand Isle State Park in Grand Isle, Louisiana. Supplemental Information: Burrowing activity: Day, Tank, Species, Oil concentration (mg/cm^2), Number of burrows, Sex, Individual, Carapace width (mm), Burrow diameter (mm), Extract volume (mL), Absorbance for TBARS, TBARS amount (nmoles), TBARS concentration (nmoles/mL), Absorbance in protein assay, Protein concentration (mg/mL), TBARS concentration (nmoles/mg protein), burrow depth (cm), amount of sediment excavated (g dry wt.), estimated sediment volume removed (cm3) |Fiddler crabs were collected at the coastal marshes and sand banks of Elmer’s Island and Grand Isle State Park in Grand Isle, Louisiana. Two indoor large holding tanks, one for each of the species, were filled with sediment and water from the collection sites. Each tank was filled using one 10-gallon bucket of sediment and approximately 5 liters of water. Crabs were kept in these tanks at the University of Louisiana at Lafayette Ecology Center. The species collected were identified using the guidelines reported by Crane (1975). Experiments were conducted with Uca longisignalis and U. panacea. To evaluate burrowing behavior and oxidative stress, three experiments were conducted using 10-gallon aquaria (20”L x 10”W x 12”H) placed next to each other in two rows. The first two experiments, one for each of the species, used 15 mesocosms. The third experiment included both species at the same time and it required 18 mesocosms. The rationale for this third experiment was to avoid differences from environmental factors and to provide a direct comparison between species since the other experiments (one for each species) were carried out at different times. The aquaria used in all of the experiments were filled with approximately five gallons of sediment from the collection sites in Grand Isle, LA. Sediment was added to each tank making a shallow slope of about 22° from one side (20 cm high) to the other (base of the tank). For Uca longisignalis, sediment consisted of a mixture of approximately 1.5 gallons clayey sediment and 3.5 gallons sandy sediment with a total organic content of X%. For Uca panacea, 5 gallons of sandy sediment were used with a total organic content of X%. Water with an average salinity of 3.5 ppt was added to the side with low sediment to a maximum height of 6.5 cm. Fiddler crabs are semi-terrestrial organisms; thus, this design allowed the addition of water without completely covering the sediment surface as wet sediment impedes burrowing construction. Five different concentrations of Louisiana Sweet crude oil (GoM Marlin Platform Dorado well) were used in experiments where species were individually evaluated. The concentrations were: 0, 8, 20, 40, 55 mg of oil per cm2 of sediment surface for Uca longisignalis, and 0, 5, 15, 25 and 35 mg of oil per cm2 of sediment surface for Uca panacea. For the third experiment, only three concentrations of crude oil were used and they were selected based on the results from the previous two experiments. These concentrations were: 0, 10 and 35 mg of oil per cm2 of surface sediment. Crude oil was mixed with 100 grams (wet weight) of sediment before being added to each mesocosm. The oil-sediment mixture was evenly spread over the sediment surface avoiding direct contact with water in the aquarium. Three replicates for each concentration were established in each of the experiments. Three male and three female fiddler crabs were placed in each mesocosm for individual species and three males and two females were used per mesocosm in the third experiment. The carapace size (mm) of each fiddler crab was measured before adding them to the mesocosms. Fiddler crabs in each mesocosm were marked with a different paint color on their carapaces to keep track of them during an experiment. Each experiment lasted for 10 days, and daily monitoring was done to record sediment temperatures in all mesocosms and to add water when it was necessary. Crabs were removed from mesocosms at the end of the exposure period, placed individually in plastic bags and stored at -80°C. The experiments were conducted in a greenhouse at the Ecology Center of the University of Louisiana at Lafayette. Burrowing Frequency and Burrow Diameter Daily observations were made during the exposure period. The number of burrows in each tank was recorded on days 1, 5 and 10. Burrow diameters were measured at the end of the exposure period. Diameters were measured on the sediment surface using calipers. Two measurements were taken for each burrow, and the average of the two measurements was used for statistical analysis. Bioturbation Fiddler crab bioturbation activity was assessed by measuring the depth of burrows constructed and the volume of sediment removed by the individuals when constructing their burrows. For this purpose, 40 PVC cores of 7.5 cm diameter and 20 cm height were used. They were placed in eight rows of five cores each, and filled with moist sediment, from the fiddler crab collection sites, to a height of 15 cm. For Uca longisignalis, sediment consisted of a mixture of clayey and sandy sediment. For Uca panacea, only sandy sediment was used. Twenty individuals from each of the fiddler crab species, two concentration of crude oil (0 and 35 mg/cm2), and 10 replicates for each treatment were used. Crude oil was mixed with 50 grams (wet weight) of sediment. This mixture was evenly spread over the sediment surface in each of the treatment cores. One fiddler crab, previously measured for its carapace width, was added to each core and exposed for 5 days. Carapace width of U. longisignalis ranged between 18-21 mm, and U. panacea between 15-17 mm. Cores were covered with a mesh screen to avoid individuals climbing out. Water was daily sprayed to keep sediment moist and reduce crab mortality from desiccation. At the end of the exposure period, crabs were carefully removed from the cores. A flexible plastic strap was used to measure the depth of burrows in each core. The strap was carefully inserted into each of the burrows until the bottom was reached. A ruler was used to measure how deep the strap went. Fiddler crabs remove sediment when burrowing and pile it up next to the burrow openings. Thus, the mass of sediment dug by fiddler crabs when burrowing was used as a proxy to determine the volume of the burrows. Sediment found above the 15 cm mark was taken from the cores, placed in plastic bags and dried in an oven (Isotemp® (655G) by Fisher Scientific) at 50°C for 48 hours. The weight of dry sediment was recorded, and the volume of the burrow was calculated using the sediment density (1.72 g/cm3 for U. longisignalis and 1.57 g/cm3 for U. panacea). Oxidative Stress Oxidative stress was quantified by determining lipid peroxidation levels in the hepatopancreas. Lipid peroxidation was assessed by employing a modified TBARS assay reported by Dorts et al. (2009). Fiddler crabs were removed from the -80° freezer and dissected to obtain the hepatopancreas. A standard weight of 100 mg of tissue was placed in a clean microcentrifuge tube and homogenized with 500 µL of 0.05M potassium phosphate buffer. A 350 µL aliquot was transferred to another clean microcentrifuge tube and the same volume of 5% trichloroacetic acid (TCA) was added. The mixture was incubated in ice for 15 minutes, followed by the addition of 350 µL of 0.67% thiobarbituric acid (TBA). The solution was centrifuged at 2,200 rpm for 10 mins at 4°C. The supernatant was transferred to another clean centrifuge tube and boiled at 85-95°C. This solution was used to measure absorbance at 535 nm using a spectrophotometer (GenesysTM 20 (4001/4) by Thermo Fisher Scientific). Protein concentrations of the crude homogenates were determined using the Bio-Rad Protein Assay based on the Bradford method. Lipid peroxidation was quantified as nM TBARS per mg protein of crude homogenate, using a molar extinction coefficient of 156,000 M-1 cm-1.|(see Methods section)|(see Methods section)|Replicates were used for all experiments. Experiments in aquaria used multiple individuals per aquarium and multiple aquaria (3) per treatment. For the experiments in cores, multiple cores (10) were used per treatment.|
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Distribution info: Distribution: Distributor: Distributor: Distributor contact: Responsible party: Organisation name: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC) Contact info: Contact: Phone: Telephone: Voice: 3618253604 Address: Address: Delivery point: 6300 Ocean Drive City: Corpus Christi Administrative area: TX Postal code: 78412 Country: USA Electronic mail address: griidc@gomri.org Online Resource: Online Resource: Linkage: URL: https://data.gulfresearchinitiative.org Role: Role code: distributor Distributor format: Format: Name: xlsx Version: inapplicable File decompression technique: zip Distributor transfer options: Digital transfer options: Transfer size: 0.0693 Online: Online Resource: Linkage: URL: https://data.gulfresearchinitiative.org/data/R2.x226.000:0008 Protocol: https Name: Data Landing Page Description: GRIIDC dataset landing page Function: Online function code: information
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Metadata maintenance: Maintenance information: Maintenance and update frequency: unknown Maintenance note: This ISO metadata record was automatically generated from information provided to GRIIDC for dataset: R2.x226.000:0008 on 2021-07-29T04:07:01-05:00
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