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Mesocosm experiments with single PAH - PAH data

The Effect of Sediment Bioturbators on the Biological Degradation of Petroleum in Coastal Ecosystems

DOI:
10.7266/N7MK69WH
 
UDI:
R2.x226.000:0003
Last Update:
Feb 24 2017 14:12 UTC
 
Dataset Author(s):
Klerks, Paul, and Febee Louka
Point of Contact:
Klerks, Paul L.
University of Louisiana at Lafayette / Department of Biology
P.O.Box 42451
Lafayette, Louisiana  70504
USA
klerks@louisiana.edu
Funding Source:
RFP-II
 
Extent Description:
Dataset contains measurements of hydrocarbon concentrations in laboratory mesocosm experiments

Identified Submitted Review Available
3 3 3 3

Suggested Citation:

Klerks, Paul, and Febee Louka (2015) Mesocosm experiments with single PAH - PAH data. Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University-Corpus Christi. doi:10.7266/N7MK69WH

Abstract:

This dataset covers part of the data for experiments in which a single PAH (pyrene) was added to mesocosms. Data provided here are the measured concentrations of pyrene in sediment samples (surface and subsurface) and water samples of the mesocosms. Bioturbator data (including survival/recovery) and environmental data (D.O., redox, turbidity, etc.) for sediment and water in the mesocosms are provided in a separate dataset. The dataset contains results for an experiment with ghost shrimp and for an experiment with the razor clam as the bioturbator species.

Purpose:

Experiment aimed at determining the effect of bioturbators on the distribution and degradation of petroleum hydrocarbons in nearshore environments. This specific dataset provides data on day-1 pyrene concentrations in water, sediment from pyrene-spiked mesocosms (with or without bioturbators). It also includes also the pyrene levels in water, surface sediment and subsurface from these same mesocosms sampled at the end of the experiment (day 10 or day 15). Experiment duration differed among experiments conducted at different times of the year, as an adjustment for anticipated differences in microbial activity.

Theme Keywords:

bioturbation, benthos, oil fate and distribution, hydrocarbons, pyrene, PAHs, ghost shrimp, razor clams, intertidal, oil on beach, microbial degradation, biodegradation

File Format:

Zip file of two Excel workbooks with multiple spreadsheets

Filename:

Dataset R2x2260000003.zip (51.01 KB)

Dataset Downloads:

3

Mesocosm experiments with single PAH - PAH data



Identification Information
Distribution Information
Metadata Maintenance Information

Metadata: 
  File identifier: 
      R2.x226.000-0003-metadata.xml
  Language: 
      eng; USA
  Character set: 
    Character set code: 
      utf8
  Hierarchy level: 
    Scope code: 
      dataset
  Metadata author: 
    Responsible party: 
      Individual name: 
          Paul L. Klerks
      Organisation name: 
          University of Louisiana at Lafayette / Department of Biology
      Position name: 
          Professor
      Contact info: 
        Contact: 
          Phone: 
            Telephone: 
              Voice: 
                  3374826356
              Facsimile: 
          Address: 
            Address: 
              Delivery point: 
                  P.O.Box 42451
              City: 
                  Lafayette
              Administrative area: 
                  Louisiana
              Postal code: 
                  70504
              Country: 
                  USA
              Electronic mail address: 
                  klerks@louisiana.edu
      Role: 
        Role code: 
          pointOfContact
  Date stamp: 
      2018-08-11T13:34:47+00:00
  Metadata standard name: 
      ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
  Metadata standard version: 
      ISO 19115-2:2009(E)
  Dataset URI: 
      https://data.gulfresearchinitiative.org/metadata/R2.x226.000:0003
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Identification info: Data identification: Citation: Citation: Title: Mesocosm experiments with single PAH - PAH data Alternate title: Mesocosms with single PAH - pyrene data Date: Date: Date: 2015-10-06 Date type: Date type code: publication Abstract: This dataset covers part of the data for experiments in which a single PAH (pyrene) was added to mesocosms. Data provided here are the measured concentrations of pyrene in sediment samples (surface and subsurface) and water samples of the mesocosms. Bioturbator data (including survival/recovery) and environmental data (D.O., redox, turbidity, etc.) for sediment and water in the mesocosms are provided in a separate dataset. The dataset contains results for an experiment with ghost shrimp and for an experiment with the razor clam as the bioturbator species. Purpose: Experiment aimed at determining the effect of bioturbators on the distribution and degradation of petroleum hydrocarbons in nearshore environments. This specific dataset provides data on day-1 pyrene concentrations in water, sediment from pyrene-spiked mesocosms (with or without bioturbators). It also includes also the pyrene levels in water, surface sediment and subsurface from these same mesocosms sampled at the end of the experiment (day 10 or day 15). Experiment duration differed among experiments conducted at different times of the year, as an adjustment for anticipated differences in microbial activity. Status: Progress code: completed Point of contact: Responsible party: Individual name: Paul L. Klerks Organisation name: University of Louisiana at Lafayette / Department of Biology Position name: Professor Contact info: Contact: Phone: Telephone: Voice: 3374826356 Facsimile: Address: Address: Delivery point: P.O.Box 42451 City: Lafayette Administrative area: Louisiana Postal code: 70504 Country: USA Electronic mail address: klerks@louisiana.edu Role: Role code: pointOfContact Descriptive keywords: Keywords: Keyword: bioturbation Keyword: benthos Keyword: oil fate and distribution Keyword: hydrocarbons Keyword: pyrene Keyword: PAHs Keyword: ghost shrimp Keyword: razor clams Keyword: intertidal Keyword: oil on beach Keyword: microbial degradation Keyword: biodegradation Type: Keyword type code: theme Descriptive keywords: Keywords: Keyword: Gulf of Mexico Keyword: coastline Type: Keyword type code: place Language: eng; USA Topic category: Topic category code: oceans Topic category: Topic category code: biota Topic category: Topic category code: environment Extent: Extent: Description: Dataset contains measurements of hydrocarbon concentrations in laboratory mesocosm experiments Temporal element: Temporal extent: Extent: Time period: Description: Begin date: 2014-11-01 End date: 2015-11-30 Supplemental Information: Experimental design worksheet includes details on when the experiment was conducted, when sampling was conducted (for environmental variables and petroleum hydrocarbon) and what tanks received which of 4 treatments (bioturbator present/absent * pyrene added or not). PAH worksheet has info on concentrations of pyrene in water (μg/L), surface and subsurface sediments (μg/kg) in specific tanks. It also includes the mean concentrations as well as # of trials.|Water samples of approximately 500 mL were collected from the mesocosms (at approximately mid-depth of the water column) using a sampling device with glass tubing and Erlenmeyer flask where air displacement from the flask resulted in water flowing through the tubing from mesocosms into flask. Samples were placed in 500-mL glass containers and transported to the lab in a cooler. Samples were stored at -4 °C for a maximum of 24 hours. Hydrocarbons were extracted using liquid-liquid extraction. Dichloromethane (DCM) was used for extraction, the organic solvent containing the extracted PAH was then evaporated using a rotary evaporator at 22°C, and the residue was dissolved in hexane. The hexane was then concentrated in a vial to a volume of 1.00 mL by purging nitrogen gas into the vial. An aliquot of the sample was then injected into an Agilent technologies 7820A gas chromatography System with a flame ionization detector (GC-FID) for quantification of the pyrene. Replicates of several of the samples for both treatments (with and without bioturbators) were analyzed to assess reproducibility. The collected sediment (surface and subsurface) samples were collected with a glass coring tube, transferred to the lab in a cooler, and frozen upon arrival. All samples were freeze dried using an SP Scientific freeze dryer (Sentry 2.0). Dry samples were homogenized using mortar and pestle, and 20 g of sediment was placed in Teflon microwave tubes with 25 mL of DCM. One Touch Technology MARS microwave extraction was performed using the US EPA 3546 method. The DCM containing the extracted PAH was then filtered by gravity filtration and the remaining sediment was then washed several times with DCM, the DCM was filtered again, and combined in order to recover all the extracted pyrene. The DCM was evaporated using a rotary evaporator. The remaining residue was then dissolved in hexane. The round-bottom flask was rinsed with hexane several times in order to maximize recovery of PAH in the sample. All the hexane rinses were combined and concentrated in a vial to a volume of 1.00 mL by purging nitrogen gas into the vial. An aliquot of each extracted sample was injected immediately into the GC-FID or was frozen for analysis on the following day. The injections were done using the following conditions: The injector and detector temperatures were set at 250 and 300ᵒC, respectively; helium was used as the carrier gas; samples were injected in the splitless mode; the oven temperature was programmed from a 60ᵒC initial temperature to increase to 300ᵒC at a rate of 5ᵒC /min, was then decreased to 290ᵒC, and was maintained at this temperature for 25 minutes.|Instrumentation information is provided in the methods section.|The experiment lasted 10 or 15 days. Water and surface sediment samples were collected on day 1. Water, surface and subsurface sediments were collected on the final day (day 10 or 15).|The Gas Chromatography instrument was calibrated prior to every use. Calibration curve was obtained using Restek pyrene standard with exact concentrations. An internal standard was added to all samples prior to analyses. Replicates of samples in presence and in absence of bioturbators were performed for accuracy, as were analyses of samples from mesocosms to which no pyrene had been added. |Dataset R2.x226.000:0001 contains the experimental design and bioturbator information from these experiments, along with the environmental data for the water and sediment in the mesocosms during the experiment.
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Distribution info: Distribution: Distributor: Distributor: Distributor contact: Responsible party: Organisation name: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC) Contact info: Contact: Phone: Telephone: Voice: 3618253604 Address: Address: Delivery point: 6300 Ocean Drive City: Corpus Christi Administrative area: TX Postal code: 78412 Country: USA Electronic mail address: griidc@gomri.org Online Resource: Online Resource: Linkage: URL: https://data.gulfresearchinitiative.org Role: Role code: distributor Distributor format: Format: Name: Zip file of two Excel workbooks with multiple spreadsheets Version: inapplicable File decompression technique: unzip Distributor transfer options: Digital transfer options: Transfer size: 0.051 Online: Online Resource: Linkage: URL: https://data.gulfresearchinitiative.org/data/R2.x226.000:0003 Protocol: https Name: Data Landing Page Description: GRIIDC dataset landing page Function: Online function code: information
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Metadata maintenance: Maintenance information: Maintenance and update frequency: unknown Maintenance note: This ISO metadata record was automatically generated from information provided to GRIIDC for dataset: R2.x226.000:0003 on 2019-06-19T09:45:03+00:00
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