Mesocosm experiment with single PAH - experimental design, mesocosm water and sediment environmental data, bioturbator data

The Effect of Sediment Bioturbators on the Biological Degradation of Petroleum in Coastal Ecosystems

DOI:
10.7266/N7W093WS
 
UDI:
R2.x226.000:0001
Last Update:
Feb 23 2017 19:37 UTC
 
Dataset Author(s):
Klerks, Paul
Point of Contact:
Klerks, Paul L.
University of Louisiana at Lafayette / Department of Biology
P.O.Box 42451
Lafayette, Louisiana  70504
USA
klerks@louisiana.edu
Funding Source:
RFP-II
 
Extent Description:
Dataset contains laboratory measurements. Field sampling is limited to collection of sediment and test species. Clams and associated sediment were collected 9/27/14 from Choctoawhachee Bay, FL (30.38854 N, -86.17429 W). Shrimp and associated sediments were collected 10/26/14 from Bay St. Louis, MS (30.25658 N, -89.41510 W).

Identified Submitted In-Review Available
3 3 3 3

Suggested Citation:

Klerks, Paul (2015) Mesocosm experiment with single PAH - experimental design, mesocosm water and sediment environmental data, bioturbator data. Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University-Corpus Christi. doi:10.7266/N7W093WS

Abstract:

This dataset covers part of the data for experiments in which a single PAH was added to mesocosms. Data provided here are experimental design, bioturbator data (including survival/recovery) and environmental data (D.O., redox, turbidity, etc.) for sediment and water in the mesocosms. Data on PAH levels in water and sediment will be provided in a separate dataset.

Purpose:

Experiment aimed at determining the effect of bioturbators on the distribution and degradation of petroleum hydrocarbons in nearshore environments. This specific dataset provides data on experimental design and bioturbator information (as background information) and data on environmental variables measured in the mesocosms. The environmental data are meant to provide insights on how the bioturbators may be affecting the distribution and biodegradation of the hydrocarbons.

Theme Keywords:

petroleum hydrocarbons, biodegradation, fate and distribution, mesocosms, bioturbation, pyrene, PAHs

File Format:

xlsx

Filename:

water_sed_quality_data_mesocosm_exps.zip (138.83 KB)

Dataset Downloads:

2

Mesocosm experiment with single PAH - experimental design, mesocosm water and sediment environmental data, bioturbator data



Identification Information
Distribution Information
Metadata Maintenance Information

Metadata: 
  File identifier: 
      R2.x226.000-0001-metadata.xml
  Language: 
      eng; USA
  Character set: 
    Character set code: 
      utf8
  Hierarchy level: 
    Scope code: 
      dataset
  Metadata author: 
    Responsible party: 
      Individual name: 
          Paul L. Klerks
      Organisation name: 
          University of Louisiana at Lafayette / Department of Biology
      Position name: 
          Professor
      Contact info: 
        Contact: 
          Phone: 
            Telephone: 
              Voice: 
                  3374826356
              Facsimile: 
          Address: 
            Address: 
              Delivery point: 
                  P.O.Box 42451
              City: 
                  Lafayette
              Administrative area: 
                  Louisiana
              Postal code: 
                  70504
              Country: 
                  USA
              Electronic mail address: 
                  klerks@louisiana.edu
      Role: 
        Role code: 
          pointOfContact
  Date stamp: 
      2018-08-11T13:35:29+00:00
  Metadata standard name: 
      ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
  Metadata standard version: 
      ISO 19115-2:2009(E)
  Dataset URI: 
      https://data.gulfresearchinitiative.org/metadata/R2.x226.000:0001
Return To Index

Identification info: Data identification: Citation: Citation: Title: Mesocosm experiment with single PAH - experimental design, mesocosm water and sediment environmental data, bioturbator data Alternate title: Mesocosm experiment basic and environmental data, single PAH experiment. Date: Date: Date: 2015-08-04 Date type: Date type code: publication Abstract: This dataset covers part of the data for experiments in which a single PAH was added to mesocosms. Data provided here are experimental design, bioturbator data (including survival/recovery) and environmental data (D.O., redox, turbidity, etc.) for sediment and water in the mesocosms. Data on PAH levels in water and sediment will be provided in a separate dataset. Purpose: Experiment aimed at determining the effect of bioturbators on the distribution and degradation of petroleum hydrocarbons in nearshore environments. This specific dataset provides data on experimental design and bioturbator information (as background information) and data on environmental variables measured in the mesocosms. The environmental data are meant to provide insights on how the bioturbators may be affecting the distribution and biodegradation of the hydrocarbons. Status: Progress code: completed Point of contact: Responsible party: Individual name: Paul L. Klerks Organisation name: University of Louisiana at Lafayette / Department of Biology Position name: Professor Contact info: Contact: Phone: Telephone: Voice: 3374826356 Facsimile: Address: Address: Delivery point: P.O.Box 42451 City: Lafayette Administrative area: Louisiana Postal code: 70504 Country: USA Electronic mail address: klerks@louisiana.edu Role: Role code: pointOfContact Descriptive keywords: Keywords: Keyword: petroleum hydrocarbons Keyword: biodegradation Keyword: fate and distribution Keyword: mesocosms Keyword: bioturbation Keyword: pyrene Keyword: PAHs Type: Keyword type code: theme Descriptive keywords: Keywords: Keyword: inapplicable Type: Keyword type code: place Language: eng; USA Topic category: Topic category code: oceans Topic category: Topic category code: biota Topic category: Topic category code: environment Extent: Extent: Description: Dataset contains laboratory measurements. Field sampling is limited to collection of sediment and test species. Clams and associated sediment were collected 9/27/14 from Choctoawhachee Bay, FL (30.38854 N, -86.17429 W). Shrimp and associated sediments were collected 10/26/14 from Bay St. Louis, MS (30.25658 N, -89.41510 W). Supplemental Information: Experimental design worksheet includes details on when the experiment was conducted, when sampling was conducted (for environmental variables and petroleum hydrocarbons) and what tanks received which of 4 treatments (bioturbator present/absent * pyrene added or not). Bioturbator worksheet has info on bioturbator identity/source/experimental density, recap of treatment assignments to specific tanks, and listing of the number of bioturbators recovered at the end of the experiment. Clam size (cm) and % survival are also included. Turbidity worksheet has turbidity results (in NTU) by observation day and tank. Redox worksheet has redox measurement results (in mV), 3 replicate measurements and mean, by observation day and tank. D.O. worksheet has ODO measurement results (in % saturation and mg/L D.O.) and probe-adjusted data (since measurements were conducted with separate probes, in clean tanks and pyrene-dosed tanks, to avoid cross-contamination). The same worksheet has daily results from measurements on a single sample using both probes - for establishing probe corrections). ODO measurements are listed by observation day and tank. Temperature worksheet has measurements for water temperature (in *C; duplicate measurements and means), measurements for sediment temperature (in *C; duplicate measurements and means), the difference in temperature between water and sediment of the same tank (Delta T in °C), and the absolute temperature difference (Delta T abs in °C). Temperature data are listed by observation day and tank. |Water samples of approximately 30 mL were collected from the tanks (at approximately mid-depth of the water column) using a turkey baster and placed in 30-mL glass tubes for transport to the lab. In the lab, water samples were transferred to glass tubes and turbidity quantified on a Hach 2100A turbidimeter, calibrated daily with GELEX® secondary turbidity standards. Turbidity was expressed in nephelometric turbidity units (NTU). Redox measurements were obtained with a custom made redox electrode with a platinum indicator electrode exposed at the end of a thin rigid shaft and a Corning calomel reference probe, using a portable pH/volt meter (Cole Parmer Digi-Sense® pH/mV/Temp). The bottom half of the reference electrode was placed in the water column, while the indicator electrode was pushed into the sediment such that its exposed platinum end was at mid-depth of the sediment. Mid-depth values were determined at 3 locations in each tank. Redox readings were allowed to stabilize (“stable” if no change within 5 seconds) prior to recording a redox value. Dissolved oxygen (D.O.) measurements were obtained with the YSI ProODO meter. Optical D.O. readings were taken at the middle of the water column depth in each tank, and D.O. recorded in “% saturation” and “mg/L O2”. Different probes were used for clean tanks and tanks to which petroleum hydrocarbons were added. The correct for differences between the probes, measurements were taken with both probes on a single water sample each day that water quality measurements were done. Probe-adjusted ODO values were calculated on the basis of the average differences between the two probes for these water samples. Temperature measurements were obtained with a Fisher Scientific NiCr/NiAl T/C type K probe (0.1 °C resolution) with the Traceable® type K meter. On each tank, two measurements were taken in the middle of the water column and two measurements were taken at mid-depth of the sediment. Different probes were used for clean tanks and those to which petroleum hydrocarbons were added. Since the goal was to quantify differences between the sediment and the water column within tanks (obtained with the same probe), no corrections were needed for between-probe differences.|Instrumentation information is provided in the methods section.|The experiment lasted 10 days. Environmental samples were taken on 6 days during the 10-day period; 3 samples during mornings and 3 samples during afternoons (in order to capture some of the within-day variability).|Instruments were calibrated prior to use. Data entry into digital files was duplicated.|Dataset R2.x226.000:0003 will contain the results from the hydrocarbon analyses of the sediment and water samples from these experiments.
Return To Index

Distribution info: Distribution: Distributor: Distributor: Distributor contact: Responsible party: Organisation name: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC) Contact info: Contact: Phone: Telephone: Voice: 3618253604 Address: Address: Delivery point: 6300 Ocean Drive City: Corpus Christi Administrative area: TX Postal code: 78412 Country: USA Electronic mail address: griidc@gomri.org Online Resource: Online Resource: Linkage: URL: https://data.gulfresearchinitiative.org Role: Role code: distributor Distributor format: Format: Name: xlsx Version: inapplicable File decompression technique: zip Distributor transfer options: Digital transfer options: Transfer size: 0.1388 Online: Online Resource: Linkage: URL: https://data.gulfresearchinitiative.org/data/R2.x226.000:0001 Protocol: https Name: Data Landing Page Description: GRIIDC dataset landing page Function: Online function code: information
Return To Index

Metadata maintenance: Maintenance information: Maintenance and update frequency: unknown Maintenance note: This ISO metadata record was automatically generated from information provided to GRIIDC for dataset: R2.x226.000:0001 on 2019-11-13T21:39:16-06:00
Return To Index