Dataset For: Responses of bay anchovy (Anchoa mitchilli) larvae under lethal and sub-lethal scenarios of crude oil exposure

Defining Ecologically Relevant Sublethal Effects: How do Low Levels of Exposure to Oil and Dispersants Affect Performance and Survival of Larvae of Gulf Nekton?

DOI:
10.7266/N7416V3Z
 
UDI:
R2.x223.000:0001
Last Update:
Sep 06 2016 15:42 UTC
 
Dataset Author(s):
Ed Chesney
Point of Contact:
Chesney, Edward
Louisiana Universities Marine Consortium (LUMCON) / DeFelice Marine Center
8124 Hwy 56
Chauvin, Louisiana  70344
USA
echesney@lumcon.edu
Funding Source:
RFP-II
 
Extent Description:
Laboratory experiments and thus no relevant geographic or temporal extents.

Identified Submitted In-Review Available
3 3 3 3

Suggested Citation:

Ed Chesney. 2016. Dataset For: Responses of bay anchovy (Anchoa mitchilli) larvae under lethal and sub-lethal scenarios of crude oil exposure. Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7416V3Z

Abstract:

Two types of experiments were conducted using Bay anchovy (Anchoa mitchilli): In exposure experiments, larvae were exposed to high energy water accommodated fractions (HEWAF) and chemically-enhanced WAF (CEWAF) at two stages of larval development (5 and 21 days post hatch, dph) and numbers of live, dead, and moribund larvae were enumerated. In growth rate experiments, larvae were exposed to low concentrations of CEWAF for 24 hours, grown for 6 days in clean water and then assessed for mortality, and several measures of size (notochord length, total length, dry weight). Samples from the exposure experiments were analyzed for alkane and aromatic hydrocarbons.

Purpose:

Bay anchovy (Anchoa mitchilli) is an ecologically important zooplanktivorous fish inhabiting estuaries of the Gulf of Mexico and eastern North America from Maine to Florida. Because they have a protracted spawning season (spring through fall) and are abundant at all life stages in coastal estuaries, their eggs and larvae likely encountered oil that reached the coast during the Deepwater Horizon oil spill. We compared responses to oil exposure at different life stages and at lethal and sub-lethal exposure conditions using acute, 24 h exposures.

Theme Keywords:

Bay Anchovy, exposures, crude oil, HEWAF, CEWAF, mortality, sub-lethal effects, growth, development, estuary

File Format:

xlsx

Filename:

R2.x223.000-0001_Chesney anchovy-GCMS data_sub 1.xlsx (584.88 KB)

Dataset Downloads:

12

Dataset For: Responses of bay anchovy (Anchoa mitchilli) larvae under lethal and sub-lethal scenarios of crude oil exposure



Identification Information
Distribution Information
Metadata Maintenance Information

Metadata: 
  File identifier: 
      R2.x223.000-0001-metadata.xml
  Language: 
      eng; USA
  Character set: 
    Character set code: 
      utf8
  Hierarchy level: 
    Scope code: 
      dataset
  Metadata author: 
    Responsible party: 
      Individual name: 
          Edward Chesney
      Organisation name: 
          Louisiana Universities Marine Consortium (LUMCON) / DeFelice Marine Center
      Position name: 
          Associate Professor
      Contact info: 
        Contact: 
          Phone: 
            Telephone: 
              Voice: 
                  9858512838
              Facsimile: 
          Address: 
            Address: 
              Delivery point: 
                  8124 Hwy 56
              City: 
                  Chauvin
              Administrative area: 
                  Louisiana
              Postal code: 
                  70344
              Country: 
                  USA
              Electronic mail address: 
                  echesney@lumcon.edu
      Role: 
        Role code: 
          principalInvestigator
  Date stamp: 
      2021-05-04T20:04:57+00:00
  Metadata standard name: 
      ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
  Metadata standard version: 
      ISO 19115-2:2009(E)
  Dataset URI: 
      https://data.gulfresearchinitiative.org/metadata/R2.x223.000:0001
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Identification info: Data identification: Citation: Citation: Title: Dataset For: Responses of bay anchovy (Anchoa mitchilli) larvae under lethal and sub-lethal scenarios of crude oil exposure Alternate title: Date: Date: Date: 2016-09-08 Date type: Date type code: publication Identifier: Identifier: Code: Anchor: xlink: https://dx.doi.org/10.7266/N7416V3Z title: DOI doi:10.7266/N7416V3Z Abstract: Two types of experiments were conducted using Bay anchovy (Anchoa mitchilli): In exposure experiments, larvae were exposed to high energy water accommodated fractions (HEWAF) and chemically-enhanced WAF (CEWAF) at two stages of larval development (5 and 21 days post hatch, dph) and numbers of live, dead, and moribund larvae were enumerated. In growth rate experiments, larvae were exposed to low concentrations of CEWAF for 24 hours, grown for 6 days in clean water and then assessed for mortality, and several measures of size (notochord length, total length, dry weight). Samples from the exposure experiments were analyzed for alkane and aromatic hydrocarbons. Purpose: Bay anchovy (Anchoa mitchilli) is an ecologically important zooplanktivorous fish inhabiting estuaries of the Gulf of Mexico and eastern North America from Maine to Florida. Because they have a protracted spawning season (spring through fall) and are abundant at all life stages in coastal estuaries, their eggs and larvae likely encountered oil that reached the coast during the Deepwater Horizon oil spill. We compared responses to oil exposure at different life stages and at lethal and sub-lethal exposure conditions using acute, 24 h exposures. Status: Progress code: completed Point of contact: Responsible party: Individual name: Edward Chesney Organisation name: Louisiana Universities Marine Consortium (LUMCON) / DeFelice Marine Center Position name: Associate Professor Contact info: Contact: Phone: Telephone: Voice: 9858512838 Facsimile: Address: Address: Delivery point: 8124 Hwy 56 City: Chauvin Administrative area: Louisiana Postal code: 70344 Country: USA Electronic mail address: echesney@lumcon.edu Role: Role code: principalInvestigator Descriptive keywords: Keywords: Keyword: Bay Anchovy Keyword: exposures Keyword: crude oil Keyword: HEWAF Keyword: CEWAF Keyword: mortality Keyword: sub-lethal effects Keyword: growth Keyword: development Keyword: estuary Type: Keyword type code: theme Descriptive keywords: Keywords: Keyword: inapplicable Type: Keyword type code: place Resource constraints: title: Cite As Constraints: Use limitation: Ed Chesney. 2016. Dataset For: Responses of bay anchovy (Anchoa mitchilli) larvae under lethal and sub-lethal scenarios of crude oil exposure. Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7416V3Z Resource constraints: title: CC0 License Legal constraints: Use constraints: Restriction code: licenceUnrestricted Other constraints: This information is released under the Creative Commons license - No Rights Reserved - CC0 1.0 Universal (https://creativecommons.org/publicdomain/zero/1.0/). The person who associated a work with this deed has dedicated the work to the public domain by waiving all of his or her rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law. You can copy, modify, distribute and perform the work, even for commercial purposes, all without asking permission. Resource constraints: title: Liability and Warranty Legal constraints: Other constraints: All materials on this website are made available to GRIIDC and in turn to you "as-is." Content may only be submitted by an individual who represents and warrants that s/he has sufficient rights to be able to make the content available under a CC0 waiver. There is no warranty (expressed or implied) to these materials, their title, accuracy, non-infringement of third party rights, or fitness for any particular purpose, including the performance or results you may obtain from their use. Use these materials at your own risk. Under no circumstances shall GRIIDC be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use or the inability to use either this website or the materials available via this website. If you are dissatisfied with any website feature, content, or terms of use, your sole and exclusive remedy is to discontinue use. Aggregation Info: AggregateInformation: Aggregate Data Set Name: title: Related Publication Citation Citation: Title: Duffy, T. A., Childress, W., Portier, R., & Chesney, E. J. (2016). Responses of bay anchovy (Anchoa mitchilli) larvae under lethal and sublethal scenarios of crude oil exposure. Ecotoxicology and Environmental Safety, 134, 264–272. doi:10.1016/j.ecoenv.2016.08.010 Date: inapplicable Aggregate Data Set Identifier: title: Related Publication DOI Identifier: Code: Anchor: xlink: https://dx.doi.org/10.1016/j.ecoenv.2016.08.010 title: DOI doi:10.1016/j.ecoenv.2016.08.010 Association Type: Association type code: crossReference Language: eng; USA Topic category: Topic category code: biota Topic category: Topic category code: environment Extent: Extent: Description: Laboratory experiments and thus no relevant geographic or temporal extents. Supplemental Information: File: R2.x223.000-001_Chesney anchovy-GCMS data-sub 1.xlsx Worksheet: Exposure Experiments Attribute_Label: Experiment Type Attribute_Definition: Identifier for exposure type (CEWAF or HEWAF) Attribute_Label: Experiment Begin Date Attribute_Definition: Date the 24 hour exposure experiment started (MM/DD/YYYY) Attribute_Label: Nominal concentration of treatment Attribute_Definition: For CEWAF experiments, units are mg/L, for HEWAF experiments, units are % Attribute_Label: Species tested Attribute_Definition: Type of organism used in experiment Attribute_Label: Age of larvae at start of experiment (dph) Attribute_Definition: Age of larvae used in each experiment, expressed in days post hatch or “dph” Attribute_Label: RFU reading 1 Attribute_Definition: Relative Fluorescence Units for the 1st of 3 readings Attribute_Label: RFU reading 2 Attribute_Definition: Relative Fluorescence Units for the 2nd of 3 readings Attribute_Label: RFU reading 3 Attribute_Definition: Relative Fluorescence Units for the 3rd of 3 readings Attribute_Label: AVG RFU Attribute_Definition: Average value of 3 RFU readings for each treatment Attribute_Label: Replicate Designation Attribute_Definition: User identified replicate identifier Attribute_Label: Temperature (deg C) Attribute_Definition: Temperature of each treatment, expressed in degrees Celsius Attribute_Label: Dissolved Oxygen (mg/L) Attribute_Definition: Dissolved oxygen of each treatment, expressed in mg per Liter Attribute_Label: # of Live individuals Attribute_Definition: Number of live larvae remaining at the end of the exposure experiment Attribute_Label: # of Dead individuals Attribute_Definition: Number of dead larvae at the end of the exposure experiment Attribute_Label: # of Moribund individuals Attribute_Definition: Number of larvae that appeared to be approaching death at the end of the exposure experiment Attribute_Label: Total number recovered Attribute_Definition: Sum total of larvae at the end of the exposure experiment, regardless of condition Attribute_Label: Ammonia Attribute_Definition: Ammonia present in the treatment Attribute_Label: pH Attribute_Definition: pH levels in the treatment (unitless) Attribute_Label: Salinity Attribute_Definition: Salinity of the treatment, expressed in psu Attribute_Label: Notes Attribute_Definition: User defined field to hold relevant information relating to the experiments Worksheet: Growth Rate Experiments Attribute_Label: Experiment Type Attribute_Definition: Identifier for exposure type (CEWAF or HEWAF) Attribute_Label: Trial Begin Date Attribute_Definition: Date the 6 day grow out experiment started (MM/DD/YYYY) Attribute_Label: Nominal concentration of treatment Attribute_Definition: For CEWAF experiments, units are mg/L Attribute_Label: Timing of larval measurements Attribute_Definition: Two larval measurements were made: a subset after a 24 h exposure, another subset after a 6 day grow out period Attribute_Label: Species tested Attribute_Definition: Type of organism used in experiment Attribute_Label: Age of larvae at start of experiment (dph) Attribute_Definition: Age of larvae used in each experiment, expressed in days post hatch or “dph” Attribute_Label: Replicate Designation Attribute_Definition: User identified replicate identifier Attribute_Label: Trial #-Fish Tin # Attribute_Definition: User defined designator for Trial # and randomly assigned number of the tin weighing dishes Attribute_Label: Empty tin wt (mg) Attribute_Definition: Weight of the randomly assigned weighing dish before weighing of the larvae. Unit = milligrams. Attribute_Label: dry fish wt + tin (mg) Attribute_Definition: Weight of the randomly assigned weighing dish with the dried larvae. Unit = milligrams. Attribute_Label: notochord length Attribute_Definition: Length of the larval notochords, expressed in units of the eyepiece micrometer Attribute_Label: Total Length Attribute_Definition: Total length of the larvae, expressed in units of the eyepiece micrometer Attribute_Label: Magnification Attribute_Definition: Magnification at which the length measurements were obtained Attribute_Label: # live larvae remaining after 6 day grow out Attribute_Definition: Number of larvae alive in each treatment at the end of the grow out experiment Attribute_Label: Conversion factor for the magnification used (mm/unit) Attribute_Definition: Actual measurement of each unit of the eyepiece micrometer, expressed in millimeters Attribute_Label: Notochord length (mm) Attribute_Definition: Length of the larval notochords, expressed in mm (obtained by multiplying number of eyepiece micrometer units counted for the notochord times conversion factor) Attribute_Label: Total length (mm) Attribute_Definition: Total length of the larva, expressed in mm (obtained by multiplying number of eyepiece micrometer units counted for the total length times conversion factor) Worksheet: GC-MS Aromatic data compiled Parameter Descriptions: Client ID-Technician designation (Informal sample designation given by the instrument technician); Nominal conc (Nominal concentration - for CEWAF experiments units are mg/L, for HEWAF experiments, units are %); Oil Preparation Type (indicates CEWAF or HEWAF experiments); Days post hatch (Age of the larvae used in the experiment); AITL ID (Identifier assigned by the analytical lab to each sample); Sample Matrix (Sample type - Sediment or Water); Analytical Instrument (Instrument used in analytical lab); Sample Size (Volume of sample analyzed); Sample Unit-Basis (Units for volume of sample analyzed); Units (Units for oil analyte results); concentration of 43 aromatics (μg/L - microgram per liter); Surrogate Recovery (%) Worksheet: GC-MS Alkane data compiled Parameter Descriptions: Client ID-Technician designation (Informal sample designation given by the instrument technician); Nominal conc (Nominal concentration - for CEWAF experiments units are mg/L, for HEWAF experiments, units are %); Oil Preparation Type (indicates CEWAF or HEWAF experiments); Days post hatch (Age of the larvae used in the experiment); AITL ID (Identifier assigned by the analytical lab to each sample); Sample Matrix (Sample type - Sediment or Water); Analytical Instrument (Instrument used in analytical lab); Sample Size (Volume of sample analyzed); Sample Unit-Basis (Units for volume of sample analyzed); Units (Units for oil analyte results); concentration of 28 alkanes (μg/L - microgram per liter); Surrogate Recovery (%) |Exposure experiments: Bay anchovy larvae (~5 or ~ 21 days post hatch) were exposed to HEWAF or CEWAF treatments of various concentrations for 24 hours and numbers of live, dead, and moribund larvae were enumerated. Ammonia and pH were randomly measured on one or two treatments per experiment after it was demonstrated in trial experiments that these did not change over the course of the experiments. Rotifer densities were estimated at the beginning of each experiment and the appropriate volume was added to reach the same density for each trial. Dissolved oxygen (DO) and Temperature were measured at time of experiment breakdown (~23.5 h). RFUs were collected after the stock solution was made, once per treatment concentration, and the volume then split among duplicate treatments. Growth rate experiments: Larvae were exposed to low concentrations of CEWAF for 24 hours, grown for 6 days in clean water and then assessed for mortality, and several measures of size (notochord length, total length, dry weight).||50 fish were added to each treatment container, except for the 4/7/14 exposure experiment, when 30 fish were used.||
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Distribution info: Distribution: Distributor: Distributor: Distributor contact: Responsible party: Organisation name: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC) Contact info: Contact: Phone: Telephone: Voice: 3618253604 Address: Address: Delivery point: 6300 Ocean Drive City: Corpus Christi Administrative area: TX Postal code: 78412 Country: USA Electronic mail address: griidc@gomri.org Online Resource: Online Resource: Linkage: URL: https://data.gulfresearchinitiative.org Role: Role code: distributor Distributor format: Format: Name: xlsx Version: inapplicable File decompression technique: Distributor transfer options: Digital transfer options: Transfer size: 0.5849 Online: Online Resource: Linkage: URL: https://data.gulfresearchinitiative.org/data/R2.x223.000:0001 Protocol: https Name: Data Landing Page Description: GRIIDC dataset landing page Function: Online function code: information
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Metadata maintenance: Maintenance information: Maintenance and update frequency: unknown Maintenance note: This ISO metadata record was automatically generated from information provided to GRIIDC for dataset: R2.x223.000:0001 on 2021-09-23T20:05:43-05:00
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