Oil droplet counts and volumes in fecal pellets of planktonic copepods (Temora turbinata, Acartia tonsa, and Parvocalanus crassirostris) exposed to crude oil and dispersant-treated oil emulsions

Dispersion Research on Oil: Physics and Plankton Studies (DROPPS)

DOI:
10.7266/N7028PK1
 
UDI:
R1.x140.125:0020
Last Update:
Aug 24 2016 18:22 UTC
 
Dataset Author(s):
Rodrigo Almeda
Point of Contact:
Buskey, Edward J.
The University of Texas at Austin / Department of Marine Science
Marine Science Institute 750 Channel View Drive
Port Aransas, Texas  78373
USA
ed.buskey@utexas.edu
Funding Source:
RFP-I
 
Extent Description:
Dataset contains the results of laboratory experiments, no field sampling involved.

Identified Submitted In-Review Available
3 3 3 3

Suggested Citation:

Rodrigo Almeda. 2016. Oil droplet counts and volumes in fecal pellets of planktonic copepods (Temora turbinata, Acartia tonsa, and Parvocalanus crassirostris) exposed to crude oil and dispersant-treated oil emulsions. Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7028PK1

Abstract:

Results from experiments to investigate and quantify defecation rates of crude oil by 3 species of marine planktonic copepods (Temora turbinata, Acartia tonsa, and Parvocalanus crassirostris) and a natural copepod assemblage after exposure to mechanically or chemically dispersed crude oil. Experiments consisted of 24-48 hour laboratory incubations of single species of copepods or natural copepod assemblages exposed to crude oil alone or dispersant-treated crude oil (“experimental treatments”), or in absence of crude oil (“control treatments”). To determine the presence of crude oil in fecal pellets, aliquots of fecal pellets fixed with glutaraldehyde were viewed under an epifluorescence microscope with bright-field and UV illumination. Images of fecal pellets with both bright-field and UV illumination were captured with a digital camera attached to the microscope.

Purpose:

To determine defecation rates of dispersed crude oil by adult stages of planktonic copepods, and estimate the quantitative impact of ingestion and defection of dispersed crude oil by copepods to the fate of crude oil after spills.

Theme Keywords:

Crude oil, Zooplankton, Ingestion, Fecal pellets, Oil spills

File Format:

xlsx, csv, tif

Filename:

R1.x140.1250020_GRIIDC_dataset_submission.zip (5.55 GB)

Dataset Downloads:

3

Oil droplet counts and volumes in fecal pellets of planktonic copepods (Temora turbinata, Acartia tonsa, and Parvocalanus crassirostris) exposed to crude oil and dispersant-treated oil emulsions



Identification Information
Distribution Information
Metadata Maintenance Information

Metadata: 
  File identifier: 
      R1.x140.125-0020-metadata.xml
  Language: 
      eng; USA
  Character set: 
    Character set code: 
      utf8
  Hierarchy level: 
    Scope code: 
      dataset
  Metadata author: 
    Responsible party: 
      Individual name: 
          Edward J. Buskey
      Organisation name: 
          The University of Texas at Austin / Department of Marine Science
      Position name: 
          Professor
      Contact info: 
        Contact: 
          Phone: 
            Telephone: 
              Voice: 
                  3617496794
              Facsimile: 
          Address: 
            Address: 
              Delivery point: 
                  Marine Science Institute
                  750 Channel View Drive
              City: 
                  Port Aransas
              Administrative area: 
                  Texas
              Postal code: 
                  78373
              Country: 
                  USA
              Electronic mail address: 
                  ed.buskey@utexas.edu
      Role: 
        Role code: 
          pointOfContact
  Date stamp: 
      2021-05-04T20:04:57+00:00
  Metadata standard name: 
      ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
  Metadata standard version: 
      ISO 19115-2:2009(E)
  Dataset URI: 
      https://data.gulfresearchinitiative.org/metadata/R1.x140.125:0020
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Identification info: Data identification: Citation: Citation: Title: Oil droplet counts and volumes in fecal pellets of planktonic copepods (Temora turbinata, Acartia tonsa, and Parvocalanus crassirostris) exposed to crude oil and dispersant-treated oil emulsions Alternate title: Date: Date: Date: 2016-08-30 Date type: Date type code: publication Identifier: Identifier: Code: Anchor: xlink: https://dx.doi.org/10.7266/N7028PK1 title: DOI doi:10.7266/N7028PK1 Abstract: Results from experiments to investigate and quantify defecation rates of crude oil by 3 species of marine planktonic copepods (Temora turbinata, Acartia tonsa, and Parvocalanus crassirostris) and a natural copepod assemblage after exposure to mechanically or chemically dispersed crude oil. Experiments consisted of 24-48 hour laboratory incubations of single species of copepods or natural copepod assemblages exposed to crude oil alone or dispersant-treated crude oil (“experimental treatments”), or in absence of crude oil (“control treatments”). To determine the presence of crude oil in fecal pellets, aliquots of fecal pellets fixed with glutaraldehyde were viewed under an epifluorescence microscope with bright-field and UV illumination. Images of fecal pellets with both bright-field and UV illumination were captured with a digital camera attached to the microscope. Purpose: To determine defecation rates of dispersed crude oil by adult stages of planktonic copepods, and estimate the quantitative impact of ingestion and defection of dispersed crude oil by copepods to the fate of crude oil after spills. Status: Progress code: completed Point of contact: Responsible party: Individual name: Edward J. Buskey Organisation name: The University of Texas at Austin / Department of Marine Science Position name: Professor Contact info: Contact: Phone: Telephone: Voice: 3617496794 Facsimile: Address: Address: Delivery point: Marine Science Institute 750 Channel View Drive City: Port Aransas Administrative area: Texas Postal code: 78373 Country: USA Electronic mail address: ed.buskey@utexas.edu Role: Role code: pointOfContact Descriptive keywords: Keywords: Keyword: Crude oil Keyword: Zooplankton Keyword: Ingestion Keyword: Fecal pellets Keyword: Oil spills Type: Keyword type code: theme Descriptive keywords: Keywords: Keyword: inapplicable Type: Keyword type code: place Resource constraints: title: Cite As Constraints: Use limitation: Rodrigo Almeda. 2016. Oil droplet counts and volumes in fecal pellets of planktonic copepods (Temora turbinata, Acartia tonsa, and Parvocalanus crassirostris) exposed to crude oil and dispersant-treated oil emulsions. Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7028PK1 Resource constraints: title: CC0 License Legal constraints: Use constraints: Restriction code: licenceUnrestricted Other constraints: This information is released under the Creative Commons license - No Rights Reserved - CC0 1.0 Universal (https://creativecommons.org/publicdomain/zero/1.0/). The person who associated a work with this deed has dedicated the work to the public domain by waiving all of his or her rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law. You can copy, modify, distribute and perform the work, even for commercial purposes, all without asking permission. Resource constraints: title: Liability and Warranty Legal constraints: Other constraints: All materials on this website are made available to GRIIDC and in turn to you "as-is." Content may only be submitted by an individual who represents and warrants that s/he has sufficient rights to be able to make the content available under a CC0 waiver. There is no warranty (expressed or implied) to these materials, their title, accuracy, non-infringement of third party rights, or fitness for any particular purpose, including the performance or results you may obtain from their use. Use these materials at your own risk. Under no circumstances shall GRIIDC be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use or the inability to use either this website or the materials available via this website. If you are dissatisfied with any website feature, content, or terms of use, your sole and exclusive remedy is to discontinue use. Aggregation Info: AggregateInformation: Aggregate Data Set Name: title: Related Publication Citation Citation: Title: Almeda, R., Connelly, T. L., & Buskey, E. J. (2016). How much crude oil can zooplankton ingest? Estimating the quantity of dispersed crude oil defecated by planktonic copepods. Environmental Pollution, 208, 645–654. doi:10.1016/j.envpol.2015.10.041 Date: inapplicable Aggregate Data Set Identifier: title: Related Publication DOI Identifier: Code: Anchor: xlink: https://dx.doi.org/10.1016/j.envpol.2015.10.041 title: DOI doi:10.1016/j.envpol.2015.10.041 Association Type: Association type code: crossReference Language: eng; USA Topic category: Topic category code: oceans Topic category: Topic category code: biota Extent: Extent: Description: Dataset contains the results of laboratory experiments, no field sampling involved. Supplemental Information: Dataset consists of 3 files and 1 folder (plus subfolders): FILE: data for GRIIDC_crude oil defecation by planktonic copepods.xlsx Tabs in file correspond to tables and figures in Almeda R, Connelly TL, Buskey EJ (2016) How much crude oil can zooplankton ingest? Estimating the quantity of dispersed crude oil defecated by planktonic copepods. Environmental Pollution 208:645-654 Tab Table1: prosome lengths of studied copepods in µm, mortality % of copepods in different treatments, fecal pellets produced per copepod per day, oil content per fecal pellet in ng oil per pellet, crude oil defecation rate in ng oil per copepod per day, crude oil defecation rate in µg oil per µg C copepod Tab Fig.2A: Percent of fecal pellets containing crude oil droplets after exposing copepods Temora turbinata, Acartia tonsa, Parvocalanus crassirostris and natural copepod assemblages (Community) to crude oil (Oil) or dispersant treated oil (Oil+Disp) emulsions Tab Fig.2B: Mean fecal pellet volume (µm3) after exposing copepods Temora turbinata, Acartia tonsa, Parvocalanus crassirostris and natural copepod assemblages (Community) to crude oil (Oil) or dispersant treated oil (Oil+Disp) emulsions Tab Fig.3: Mean and median size/diameter (µm) of crude oil droplets inside the fecal pellets Tab Fig.4: Mean/median number of crude oil droplets per pellet, Mean/median volume (µm3) of oil per fecal pellet Tab Fig.5: Copepod body weights (µg C) and crude oil defecation rates (ng oil per copepod per day) by species and treatment FILE: diameter and volume per oil droplet in fp.csv Diameters and volumes of individual oil droplets in all fecal pellets Column 1: Label -- image name Column 2: Major -- major axis diameter (µm) Column 3: Minor -- minor axis diameter (µm) Column 4: Treatment -- treatment name Column 5: Replicate -- replicate within treatment Column 6: vol.um3 -- calculated volume of oil droplet in µm3 (volume = 4/3*pi*(major axis/2)*(minor axis/2)^2) Column 7: Species -- copepod species FILE: total oil volume and count per fp.csv Total volume of oil and number of droplets in each fecal pellet Column 1: Treatment -- treatment name Column 2: Replicate -- replicate within treatment Column 3: Label -- image name Column 4: vol.um3.fp -- total volume of oil in fecal pellet in µm3 Column 5: Oil.count.fp -- total number of oil droplets in fecal pellet Column 6: Species -- copepod species FOLDER: fecal pellet images Bright-field and UV illumination images of copepod fecal pellets containing oil droplets. Image names correspond to the Label column in the csv data files Contains 4 subfolders (1 subfolder for each species of copepod used in the experiments) -- Temora, Parvocalanus, Acartia, Community Each subfolder contains 4 folders of fecal pellet images (1 folder for each treatment+replicate combination) -- OilA, OilB, OilDispA, OilDispB |Light Louisiana sweet crude oil and Corexit 9500A were used for the oil and oil+dispersant treatments respectively. Treatments consisted of control (no oil or dispersant), oil (exposure to 1 uL/L of crude oil), and oil + disp (exposure to 1 uL/L dispersant treated crude oil). Each treatment was run twice (replicates A and B). Species treatments (T. turbinata, P. crassirostris, and A. tonsa) were incubated 48 hrs while the community group was incubated for 24 hrs.||||
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Distribution info: Distribution: Distributor: Distributor: Distributor contact: Responsible party: Organisation name: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC) Contact info: Contact: Phone: Telephone: Voice: 3618253604 Address: Address: Delivery point: 6300 Ocean Drive City: Corpus Christi Administrative area: TX Postal code: 78412 Country: USA Electronic mail address: griidc@gomri.org Online Resource: Online Resource: Linkage: URL: https://data.gulfresearchinitiative.org Role: Role code: distributor Distributor format: Format: Name: xlsx, csv, tif Version: inapplicable File decompression technique: zip Distributor transfer options: Digital transfer options: Transfer size: 5554.6285 Online: Online Resource: Linkage: URL: https://data.gulfresearchinitiative.org/data/R1.x140.125:0020 Protocol: https Name: Data Landing Page Description: GRIIDC dataset landing page Function: Online function code: information
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Metadata maintenance: Maintenance information: Maintenance and update frequency: unknown Maintenance note: This ISO metadata record was automatically generated from information provided to GRIIDC for dataset: R1.x140.125:0020 on 2021-09-23T18:47:22-05:00
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