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Biochemical characterization of Extracellular Polymeric Substances produced by phytoplankton-associated bacteria

Center for Integrated Modeling and Analysis of Gulf Ecosystems (C-IMAGE)

DOI:
10.7266/N78C9T75
 
UDI:
R1.x135.119:0003
Last Update:
Jan 14 2016 16:50 UTC
 
Dataset Author(s):
van Eenennaam, Justine
Point of Contact:
van Eenennaam, Justine
Wageningen University / Environmental Technology
Bornse Weilanden 9
Wageningen,   6708 WG
NLD
justine.vaneenennaam@wur.nl
Funding Source:
RFP-I
 
Extent Description:
Dataset contains laboratory measurements of biochemical composition of Extracellular Polymeric substances, no field sampling involved.

Identified Submitted Metadata Available
3 3 3 3

Abstract:

Dataset contains data on the biochemical characterization (proteins, polysaccharides, monosaccharides) of Extracellular Polymeric Substances produced by phytoplankton-associated bacteria, upon exposure to Corexit 9500 oil spill dispersant.

Purpose:

Data of experiments as performed for publication in peer-reviewed journal.

Theme Keywords:

Extracellular Polymeric Substances, Marine Snow, Dispersant, Corexit

File Format:

Excel Spreadsheet

Biochemical characterization of Extracellular Polymeric Substances produced by phytoplankton-associated bacteria



Identification Information
Distribution Information
Metadata Maintenance Information

Metadata: 
  File identifier: 
      R1.x135.119-0003-metadata.xml
  Language: 
      eng; USA
  Character set: 
    Character set code: 
      utf8
  Hierarchy level: 
    Scope code: 
      dataset
  Metadata author: 
    Responsible party: 
      Individual name: 
          Justine van Eenennaam
      Organisation name: 
          Wageningen University
      Position name: 
          PhD Researcher
      Contact info: 
        Contact: 
          Address: 
            Address: 
              Delivery point: 
                  Bornse Weilanden 9
              City: 
                  Wageningen
              Administrative area: 
                  Gelderland
              Postal code: 
                  6708 WG
              Country: 
                  NLD
              Electronic mail address: 
                  justine.vaneenennaam@wur.nl
      Role: 
        Role code: 
          pointOfContact
  Metadata author: 
    Responsible party: 
      Individual name: 
          Todd Chavez
      Organisation name: 
          University of South Florida
      Position name: 
      Contact info: 
        Contact: 
          Phone: 
            Telephone: 
              Voice: 
                  31 317 485502
              Facsimile: 
          Address: 
            Address: 
              Delivery point: 
                  4202 E. Fowler Avenue LIB122
              City: 
                  Tampa
              Administrative area: 
                  FL
              Postal code: 
                  33620
              Country: 
                  USA
              Electronic mail address: 
                  tchavez@usf.edu
      Role: 
        Role code: 
          pointOfContact
  Date stamp: 
      2015-11-24T10:54:22-06:00
  Metadata standard name: 
      ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
  Metadata standard version: 
      ISO 19115-2:2009(E)
  Dataset URI: 
      https://data.gulfresearchinitiative.org/metadata/R1.x135.119:0003
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Identification info: Data identification: Citation: Citation: Title: Biochemical characterization of Extracellular Polymeric Substances produced by phytoplankton-associated bacteria Alternate title: Biochemical analysis EPS Date: Date: Date: 2015-05-18 Date type: Date type code: creation Abstract: Dataset contains data on the biochemical characterization (proteins, polysaccharides, monosaccharides) of Extracellular Polymeric Substances produced by phytoplankton-associated bacteria, upon exposure to Corexit 9500 oil spill dispersant. Purpose: Data of experiments as performed for publication in peer-reviewed journal. Status: Progress code: completed Point of contact: Responsible party: Individual name: Albertinka Murk Organisation name: Wageningen University Position name: Professor Contact info: Contact: Address: Address: Delivery point: De Dreijen-Tuinlaan 5 Building 320 City: Wageningen Administrative area: Gelderland Postal code: 6703 HE Country: NLD Electronic mail address: Tinka.Murk@wur.nl Role: Role code: pointOfContact Point of contact: Responsible party: Individual name: Justine van Eenennaam Organisation name: Wageningen University Position name: PhD Researcher Contact info: Contact: Address: Address: Delivery point: Bornse Weilanden 9 City: Wageningen Administrative area: Gelderland Postal code: 6708 WG Country: NLD Electronic mail address: justine.vaneenennaam@wur.nl Role: Role code: pointOfContact Point of contact: Responsible party: Individual name: Todd Chavez Organisation name: University of South Florida Position name: Contact info: Contact: Phone: Telephone: Voice: 31 317 485502 Facsimile: Address: Address: Delivery point: 4202 E. Fowler Avenue LIB122 City: Tampa Administrative area: FL Postal code: 33620 Country: USA Electronic mail address: tchavez@usf.edu Role: Role code: pointOfContact Descriptive keywords: Keywords: Keyword: Extracellular Polymeric Substances Keyword: Marine Snow Keyword: Dispersant Keyword: Corexit Type: Keyword type code: theme Descriptive keywords: Keywords: Keyword: inapplicable Type: Keyword type code: place Language: eng; USA Topic category: Topic category code: biota Topic category: Topic category code: environment Topic category: Topic category code: oceans Extent: Extent: Description: Dataset contains laboratory measurements of biochemical composition of Extracellular Polymeric substances, no field sampling involved. Supplemental Information: The information below is a breakdown of the datasheets in the dataset. Each datasheet has a brief description about the sheet and a list of relevant parameters for that datasheet. Sheet “Dry Weight” – Dry weight of EPS produced by (filtrates of) Phaeodactylum tricornutum and Dunaliella tertiolecta. Data fields: Treatment (species, type of treatment) ([Phaeodactylum tricornutum, Dunaliella tertiolecta], [filtrate, unfiltered]), Number of flasks combined, Total volume of flasks (ml), Weight empty cup (g), Weight dried EPS + cup (g), Weight dried EPS (mg), EPS dry weight per flask (mg), EPS dry weight (ng per ml); Sheet “Proteins” – Percent of nitrogen (measured) and percent of protein (calculated) of EPS produced by (filtrates of) Phaeodactylum tricornutum and Dunaliella tertiolecta. Data fields: Sample name [blank, standard, filtered, unfiltered, Duna, Diatom, methionine], date (MM-DD-YYYY), time (hh:mm), type [unknown, st andard deviation], weight (milligrams), protein factor (used to convert Nitrogen content into protein content: N*6.25 = protein content. The average nitrogen content of proteins is about 16 percent 1/0.16 = 6.25.), %N Total Nitrogen dry weight per sample dry weight (%), %protein Total protein dry weight per sample dry weight (%); Sheet “SDS-PAGE” – Protein polypeptides visualised by SDS-PAGE gel, % in lanes/bands based on molecular mass. Sample: unfiltered Phaeodactylum tricornutum. Data fields: Band no.(1 – 10), Band label, Mol. Wt. (KDa), Relative Front, Volume (OD), Abs. Quant., Rel. Quant., Band (%), Lane (%); Sheet “Polysaccharides – Filtrates” – Total polysaccharides of EPS samples produced by filtrates of Phaeodactylum tricornutum and Dunaliella tertiolecta. Data fields: Date (DD/MM/YYYY), Time (hh:mm:ss), Absorbance mode of Tecan i-control application and infinite 200Pro device, Temperature (degrees Celsius), Start Time (DD/MM/YYYY), End Time (DD/MM/YYYY), Absorbance (nanometers), glucose (ug/ml microgram per milliliter); Sheet “Polysaccharides – Unfiltered” – Total polysaccharides of EPS samples produced by unfiltered Phaeodactylum tricornutum and Dunaliella tertiolecta. Data fields: Date (DD/MM/YYYY), Time (hh:mm:ss), Absorbance mode of Tecan i-control application and infinite 200Pro device, Temperature (degrees Celsius), Start Time (DD/MM/YYYY), End Time (DD/MM/YYYY), Absorbance (nanometers), glucose (ug/ml microgram per milliliter); Sheet “Monosaccharides – unfiltered P.t” – Monosaccharide composition of dried EPS produced by unfiltered Phaeodactylum tricornutum. Data fields: Relative response ([Sample Name (Evi, Evii), Weight (mg), Uronic A (%w/w), Internal Standard (ug), Relative Response]), Sample Area ([Sample Name (Evi, Evii), Weight (mg), Uronic A (%w/w), Internal Standard (ug), Area]), Uronic Acid ([Sample Name (Evi, Evii), Weight (mg), Uronic A [(%w/w),(ug/ml)], Dillution (ml)]), Sugar composition in mol% ([Sample Name (Evi, Evii), Weight (mg), Rha, Fuc, Ara, Xyl, Man, Gal, Glc, Uronic acid, Total (w/w%)]), Sugar composition in w/w% ([Sample Name (Evi, Evii), Weight (mg), Rha, Fuc, Ara, Xyl, Man, Gal, Glc, Uronic acid, Total (w/w%)]); Sheet “Monosaccharides – others” – Monosaccharide composition of dried EPS produced by filtrate of Phaeodactylum tricornutum, and filtrate and unfiltered Dunaliella tertiolecta. Data fields: Relative response ([Sample Name (f.D-A, f.D-B, f.I-A, f.I-B, U.D-A, U.D-B), Weight(mg), Uronic A (%w/w), Internal Standard (ug), Relative response]), Sample Area ([Sample Name (f.D-A, f.D-B, f.I-A, f.I-B, U.D-A, U.D-B), Weight(mg), Uronic A (%w/w), Internal Standard (ug), Area]), Uronic Acid ([Sample Name (f.D-A, f.D-B, f.I-A, f.I-B, U.D-A, U.D-B),, Weight (mg), Uronic A [(%w/w),(ug/ml)], Dillution (ml)]), Sugar composition in mol% ([Sample Name (f.D-A, f.D-B, f.I-A, f.I-B, U.D-A, U.D-B), Weight (mg), Rha, Fuc, Ara, Xyl, Man, Gal, Glc, Uronic acid, Total (w/w%)]), Sugar composition in w/w% ([Sample Name (f.D-A, f.D-B, f.I-A, f.I-B, U.D-A, U.D-B), Weight (mg), Rha, Fuc, Ara, Xyl, Man, Gal, Glc, Uronic acid, Total (w/w%)]); |Proteins: as N-content (using Dumas method) x conversion factor Polysaccharides: phenol-sulphuric acid method (Dubois, adapted for microplate) Monosaccharides: alditol-acetates method. Full description can be found in publication (not yet published).||||
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Distribution info: Distribution: Distributor: Distributor: Distributor contact: Responsible party: Organisation name: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC) Position name: Contact info: Contact: Phone: Telephone: Voice: +1-361-825-3604 Facsimile: +1-361-825-2050 Address: Address: Delivery point: 6300 Ocean Drive Unit 5869 City: Corpus Christi Administrative area: Texas Postal code: 78412-5869 Country: USA Electronic mail address: griidc@gomri.org Online Resource: Online Resource: Linkage: URL: http://data.gulfresearchinitiative.org Protocol: Role: Role code: distributor Distributor format: Format: Name: Excel Spreadsheet Version: inapplicable File decompression technique: Distributor transfer options: Digital transfer options: Transfer size: 0.05 Online: Online Resource: Linkage: URL: https://data.gulfresearchinitiative.org/data/R1.x135.119:0003 Protocol:
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Metadata maintenance: Maintenance information: Maintenance and update frequency: unknown Maintenance note: This ISO metadata record was automatically generated from information provided to GRIIDC for dataset: R1.x135.119:0003 Maintenance note: This ISO metadata record was created using the 'Check and Save to File' (with form validation) function of the GRIIDC ISO 19115-2 Metadata Editor on 2015-11-23T18:01:06+00:00
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