Biochemical characterization of Extracellular Polymeric Substances produced by phytoplankton-associated bacteria

Center for Integrated Modeling and Analysis of Gulf Ecosystems (C-IMAGE)

DOI:
10.7266/N78C9T75
 
UDI:
R1.x135.119:0003
Last Update:
Jan 14 2016 16:50 UTC
 
Dataset Author(s):
van Eenennaam, Justine
Point of Contact:
Chavez, Todd A.
University of South Florida / USF Library System
4202 E. Fowler Avenue LIB122
Tampa, Florida  33620
USA
tchavez@usf.edu
Funding Source:
RFP-I
 
Extent Description:
Dataset contains laboratory measurements of biochemical composition of Extracellular Polymeric substances, no field sampling involved.

Identified Submitted In-Review Available
3 3 3 3

Suggested Citation:

van Eenennaam, Justine. 2015. Biochemical characterization of Extracellular Polymeric Substances produced by phytoplankton-associated bacteria. Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N78C9T75

Abstract:

Dataset contains data on the biochemical characterization (proteins, polysaccharides, monosaccharides) of Extracellular Polymeric Substances produced by phytoplankton-associated bacteria, upon exposure to Corexit 9500 oil spill dispersant.

Purpose:

Data of experiments as performed for publication in peer-reviewed journal.

Theme Keywords:

Extracellular Polymeric Substances, Marine Snow, Dispersant, Corexit

File Format:

Excel Spreadsheet

Filename:

Data for GRIIDC_JvE.xlsx (2.93 MB)

Dataset Downloads:

8

Biochemical characterization of Extracellular Polymeric Substances produced by phytoplankton-associated bacteria



Identification Information
Distribution Information
Metadata Maintenance Information

Metadata: 
  File identifier: 
      R1.x135.119-0003-metadata.xml
  Language: 
      eng; USA
  Character set: 
    Character set code: 
      utf8
  Hierarchy level: 
    Scope code: 
      dataset
  Metadata author: 
    Responsible party: 
      Individual name: 
          Justine van Eenennaam
      Organisation name: 
          Wageningen University / Environmental Technology
      Position name: 
          Graduate Student
      Contact info: 
        Contact: 
          Phone: 
            Telephone: 
              Voice: 
              Facsimile: 
          Address: 
            Address: 
              Delivery point: 
                  Bornse Weilanden 9
              City: 
                  Wageningen
              Administrative area: 
              Postal code: 
                  6708 WG
              Country: 
                  NLD
              Electronic mail address: 
                  Justine.vanEenennaam@gmail.com
      Role: 
        Role code: 
          pointOfContact
  Date stamp: 
      2018-08-11T13:43:11+00:00
  Metadata standard name: 
      ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
  Metadata standard version: 
      ISO 19115-2:2009(E)
  Dataset URI: 
      https://data.gulfresearchinitiative.org/metadata/R1.x135.119:0003
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Identification info: Data identification: Citation: Citation: Title: Biochemical characterization of Extracellular Polymeric Substances produced by phytoplankton-associated bacteria Alternate title: Biochemical analysis EPS Date: Date: Date: 2015-11-24 Date type: Date type code: publication Identifier: Identifier: Code: Anchor: xlink: https://dx.doi.org/10.7266/N78C9T75 title: DOI doi:10.7266/N78C9T75 Abstract: Dataset contains data on the biochemical characterization (proteins, polysaccharides, monosaccharides) of Extracellular Polymeric Substances produced by phytoplankton-associated bacteria, upon exposure to Corexit 9500 oil spill dispersant. Purpose: Data of experiments as performed for publication in peer-reviewed journal. Status: Progress code: completed Point of contact: Responsible party: Individual name: Todd A. Chavez Organisation name: University of South Florida / USF Library System Position name: Dean Contact info: Contact: Phone: Telephone: Voice: 8139747905 Facsimile: Address: Address: Delivery point: 4202 E. Fowler Avenue LIB122 City: Tampa Administrative area: Florida Postal code: 33620 Country: USA Electronic mail address: tchavez@usf.edu Role: Role code: pointOfContact Point of contact: Responsible party: Individual name: Albertinka J. Murk Organisation name: Wageningen University / Environmental Toxicology and Technology Position name: Professor Contact info: Contact: Phone: Telephone: Voice: 0317483233 Facsimile: Address: Address: Delivery point: De Elst 1 City: Wageningen Administrative area: Gelderland Postal code: 6708 WD Country: NLD Electronic mail address: Tinka.Murk@wur.nl Role: Role code: pointOfContact Point of contact: Responsible party: Individual name: Justine van Eenennaam Organisation name: Wageningen University / Environmental Technology Position name: Graduate Student Contact info: Contact: Phone: Telephone: Voice: Facsimile: Address: Address: Delivery point: Bornse Weilanden 9 City: Wageningen Administrative area: Postal code: 6708 WG Country: NLD Electronic mail address: Justine.vanEenennaam@gmail.com Role: Role code: pointOfContact Descriptive keywords: Keywords: Keyword: Extracellular Polymeric Substances Keyword: Marine Snow Keyword: Dispersant Keyword: Corexit Type: Keyword type code: theme Descriptive keywords: Keywords: Keyword: inapplicable Type: Keyword type code: place Resource constraints: title: Cite As Constraints: Use limitation: van Eenennaam, Justine. 2015. Biochemical characterization of Extracellular Polymeric Substances produced by phytoplankton-associated bacteria. Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N78C9T75 Resource constraints: title: CC0 License Legal constraints: Use constraints: Restriction code: licenceUnrestricted Other constraints: This information is released under the Creative Commons license - No Rights Reserved - CC0 1.0 Universal (https://creativecommons.org/publicdomain/zero/1.0/). The person who associated a work with this deed has dedicated the work to the public domain by waiving all of his or her rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law. You can copy, modify, distribute and perform the work, even for commercial purposes, all without asking permission. Resource constraints: title: Liability and Warranty Legal constraints: Other constraints: All materials on this website are made available to GRIIDC and in turn to you "as-is." Content may only be submitted by an individual who represents and warrants that s/he has sufficient rights to be able to make the content available under a CC0 waiver. There is no warranty (expressed or implied) to these materials, their title, accuracy, non-infringement of third party rights, or fitness for any particular purpose, including the performance or results you may obtain from their use. Use these materials at your own risk. Under no circumstances shall GRIIDC be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use or the inability to use either this website or the materials available via this website. If you are dissatisfied with any website feature, content, or terms of use, your sole and exclusive remedy is to discontinue use. Aggregation Info: AggregateInformation: Aggregate Data Set Name: title: Related Publication Citation Citation: Title: Van Eenennaam, J. S., Wei, Y., Grolle, K. C. F., Foekema, E. M., & Murk, A. J. (2016). Oil spill dispersants induce formation of marine snow by phytoplankton-associated bacteria. Marine Pollution Bulletin, 104(1-2), 294–302. doi:10.1016/j.marpolbul.2016.01.005 Date: inapplicable Aggregate Data Set Identifier: title: Related Publication DOI Identifier: Code: Anchor: xlink: https://dx.doi.org/10.1016/j.marpolbul.2016.01.005 title: DOI doi:10.1016/j.marpolbul.2016.01.005 Association Type: Association type code: crossReference Language: eng; USA Topic category: Topic category code: biota Topic category: Topic category code: environment Topic category: Topic category code: oceans Extent: Extent: Description: Dataset contains laboratory measurements of biochemical composition of Extracellular Polymeric substances, no field sampling involved. Supplemental Information: The information below is a breakdown of the datasheets in the dataset. Each datasheet has a brief description about the sheet and a list of relevant parameters for that datasheet. Sheet “Dry Weight” – Dry weight of EPS produced by (filtrates of) Phaeodactylum tricornutum and Dunaliella tertiolecta. Data fields: Treatment (species, type of treatment) ([Phaeodactylum tricornutum, Dunaliella tertiolecta], [filtrate, unfiltered]), Number of flasks combined, Total volume of flasks (ml), Weight empty cup (g), Weight dried EPS + cup (g), Weight dried EPS (mg), EPS dry weight per flask (mg), EPS dry weight (ng per ml); Sheet “Proteins” – Percent of nitrogen (measured) and percent of protein (calculated) of EPS produced by (filtrates of) Phaeodactylum tricornutum and Dunaliella tertiolecta. Data fields: Sample name [blank, standard, filtered, unfiltered, Duna, Diatom, methionine], date (MM-DD-YYYY), time (hh:mm), type [unknown, st andard deviation], weight (milligrams), protein factor (used to convert Nitrogen content into protein content: N*6.25 = protein content. The average nitrogen content of proteins is about 16 percent 1/0.16 = 6.25.), %N Total Nitrogen dry weight per sample dry weight (%), %protein Total protein dry weight per sample dry weight (%); Sheet “SDS-PAGE” – Protein polypeptides visualised by SDS-PAGE gel, % in lanes/bands based on molecular mass. Sample: unfiltered Phaeodactylum tricornutum. Data fields: Band no.(1 – 10), Band label, Mol. Wt. (KDa), Relative Front, Volume (OD), Abs. Quant., Rel. Quant., Band (%), Lane (%); Sheet “Polysaccharides – Filtrates” – Total polysaccharides of EPS samples produced by filtrates of Phaeodactylum tricornutum and Dunaliella tertiolecta. Data fields: Date (DD/MM/YYYY), Time (hh:mm:ss), Absorbance mode of Tecan i-control application and infinite 200Pro device, Temperature (degrees Celsius), Start Time (DD/MM/YYYY), End Time (DD/MM/YYYY), Absorbance (nanometers), glucose (ug/ml microgram per milliliter); Sheet “Polysaccharides – Unfiltered” – Total polysaccharides of EPS samples produced by unfiltered Phaeodactylum tricornutum and Dunaliella tertiolecta. Data fields: Date (DD/MM/YYYY), Time (hh:mm:ss), Absorbance mode of Tecan i-control application and infinite 200Pro device, Temperature (degrees Celsius), Start Time (DD/MM/YYYY), End Time (DD/MM/YYYY), Absorbance (nanometers), glucose (ug/ml microgram per milliliter); Sheet “Monosaccharides – unfiltered P.t” – Monosaccharide composition of dried EPS produced by unfiltered Phaeodactylum tricornutum. Data fields: Relative response ([Sample Name (Evi, Evii), Weight (mg), Uronic A (%w/w), Internal Standard (ug), Relative Response]), Sample Area ([Sample Name (Evi, Evii), Weight (mg), Uronic A (%w/w), Internal Standard (ug), Area]), Uronic Acid ([Sample Name (Evi, Evii), Weight (mg), Uronic A [(%w/w),(ug/ml)], Dillution (ml)]), Sugar composition in mol% ([Sample Name (Evi, Evii), Weight (mg), Rha, Fuc, Ara, Xyl, Man, Gal, Glc, Uronic acid, Total (w/w%)]), Sugar composition in w/w% ([Sample Name (Evi, Evii), Weight (mg), Rha, Fuc, Ara, Xyl, Man, Gal, Glc, Uronic acid, Total (w/w%)]); Sheet “Monosaccharides – others” – Monosaccharide composition of dried EPS produced by filtrate of Phaeodactylum tricornutum, and filtrate and unfiltered Dunaliella tertiolecta. Data fields: Relative response ([Sample Name (f.D-A, f.D-B, f.I-A, f.I-B, U.D-A, U.D-B), Weight(mg), Uronic A (%w/w), Internal Standard (ug), Relative response]), Sample Area ([Sample Name (f.D-A, f.D-B, f.I-A, f.I-B, U.D-A, U.D-B), Weight(mg), Uronic A (%w/w), Internal Standard (ug), Area]), Uronic Acid ([Sample Name (f.D-A, f.D-B, f.I-A, f.I-B, U.D-A, U.D-B),, Weight (mg), Uronic A [(%w/w),(ug/ml)], Dillution (ml)]), Sugar composition in mol% ([Sample Name (f.D-A, f.D-B, f.I-A, f.I-B, U.D-A, U.D-B), Weight (mg), Rha, Fuc, Ara, Xyl, Man, Gal, Glc, Uronic acid, Total (w/w%)]), Sugar composition in w/w% ([Sample Name (f.D-A, f.D-B, f.I-A, f.I-B, U.D-A, U.D-B), Weight (mg), Rha, Fuc, Ara, Xyl, Man, Gal, Glc, Uronic acid, Total (w/w%)]); |Proteins: as N-content (using Dumas method) x conversion factor Polysaccharides: phenol-sulphuric acid method (Dubois, adapted for microplate) Monosaccharides: alditol-acetates method. Full description can be found in publication (not yet published).||||
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Distribution info: Distribution: Distributor: Distributor: Distributor contact: Responsible party: Organisation name: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC) Contact info: Contact: Phone: Telephone: Voice: 3618253604 Address: Address: Delivery point: 6300 Ocean Drive City: Corpus Christi Administrative area: TX Postal code: 78412 Country: USA Electronic mail address: griidc@gomri.org Online Resource: Online Resource: Linkage: URL: https://data.gulfresearchinitiative.org Role: Role code: distributor Distributor format: Format: Name: Excel Spreadsheet Version: inapplicable File decompression technique: Distributor transfer options: Digital transfer options: Transfer size: 2.9266 Online: Online Resource: Linkage: URL: https://data.gulfresearchinitiative.org/data/R1.x135.119:0003 Protocol: https Name: Data Landing Page Description: GRIIDC dataset landing page Function: Online function code: information
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Metadata maintenance: Maintenance information: Maintenance and update frequency: unknown Maintenance note: This ISO metadata record was automatically generated from information provided to GRIIDC for dataset: R1.x135.119:0003 on 2021-04-18T16:29:54-05:00
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