Biodegradation of light crude oil using realistic sea water

Center for Integrated Modeling and Analysis of Gulf Ecosystems (C-IMAGE)

DOI:
10.7266/N76W9839
 
UDI:
R1.x135.119:0002
Last Update:
Jul 27 2016 13:36 UTC
 
Dataset Author(s):
Rahsepar, Shokouh
Point of Contact:
Rahsepar, Shokouh
Wageningen University / Environmental Technology
Bornse Weilanden 9
Wageningen, Gelderland  6708 WG
NLD
shokouh.rahsepar@wur.nl
Funding Source:
RFP-I
 
Extent Description:
Laboratory generated data, geographic and temporal extents are not relevant to this dataset.

Identified Submitted In-Review Available
3 3 3 3

Suggested Citation:

Rahsepar, Shokouh. 2016. Biodegradation of light crude oil using realistic sea water. Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N76W9839

Abstract:

This dataset reports petroleum hydrocarbon data and oxygen concentration data for laboratory experiments conducted to assess the relation between (aerobic) biodegradation of light crude oil, present of dispersants and presence of suspended solids (e.g clay). Experiments were conducted with no dispersant, and with dispersant to oil ratios of 1:20 and 1:100, using either weathered or crude oil and were tested in duplicate. Replicates were sampled 10 times over approximately two months for analysis of total petroleum hydrocarbons. Oxygen consumption was measured in experiments testing crude oil at different dispersant to oil ratios with the addition of bacterial cultures of Psuedodemonas putida and Rhodococcus qingshengii TUHH-12 over a period of 36 days.

Purpose:

Laboratory scale monitoring data and experimental setup that will be used to assess the relation between (aerobic) biodegradation of light crude oil, presence of dispersants, and presence of suspended solids (both mineral and organic s.a. plankton).

Theme Keywords:

aerobic oil biodegradation rate, bioavailability, oil weathering, suspended particles, EPS formation

File Format:

xlsx

Filename:

R1.x135.119-0002-Biodegradation-data.zip (923.59 KB)

Dataset Downloads:

8

Biodegradation of light crude oil using realistic sea water



Identification Information
Distribution Information
Metadata Maintenance Information

Metadata: 
  File identifier: 
      R1.x135.119-0002-metadata.xml
  Language: 
      eng; USA
  Character set: 
    Character set code: 
      utf8
  Hierarchy level: 
    Scope code: 
      dataset
  Metadata author: 
    Responsible party: 
      Individual name: 
          Richard McKenzie
      Organisation name: 
          University of South Florida / USF Library System
      Position name: 
          GIS Analyst
      Contact info: 
        Contact: 
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            Telephone: 
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                  8139742852
              Facsimile: 
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              Delivery point: 
                  4202 E. Fowler Avenue LIB122
              City: 
                  Tampa
              Administrative area: 
                  Florida
              Postal code: 
                  33620
              Country: 
                  USA
              Electronic mail address: 
                  rwmcken2@usf.edu
      Role: 
        Role code: 
          pointOfContact
  Date stamp: 
      2021-05-04T20:04:57+00:00
  Metadata standard name: 
      ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
  Metadata standard version: 
      ISO 19115-2:2009(E)
  Dataset URI: 
      https://data.gulfresearchinitiative.org/metadata/R1.x135.119:0002
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Identification info: Data identification: Citation: Citation: Title: Biodegradation of light crude oil using realistic sea water Alternate title: Date: Date: Date: 2016-07-29 Date type: Date type code: publication Identifier: Identifier: Code: Anchor: xlink: https://dx.doi.org/10.7266/N76W9839 title: DOI doi:10.7266/N76W9839 Abstract: This dataset reports petroleum hydrocarbon data and oxygen concentration data for laboratory experiments conducted to assess the relation between (aerobic) biodegradation of light crude oil, present of dispersants and presence of suspended solids (e.g clay). Experiments were conducted with no dispersant, and with dispersant to oil ratios of 1:20 and 1:100, using either weathered or crude oil and were tested in duplicate. Replicates were sampled 10 times over approximately two months for analysis of total petroleum hydrocarbons. Oxygen consumption was measured in experiments testing crude oil at different dispersant to oil ratios with the addition of bacterial cultures of Psuedodemonas putida and Rhodococcus qingshengii TUHH-12 over a period of 36 days. Purpose: Laboratory scale monitoring data and experimental setup that will be used to assess the relation between (aerobic) biodegradation of light crude oil, presence of dispersants, and presence of suspended solids (both mineral and organic s.a. plankton). Status: Progress code: completed Point of contact: Responsible party: Individual name: Shokouh Rahsepar Organisation name: Wageningen University / Environmental Technology Position name: Graduate Student Contact info: Contact: Phone: Telephone: Voice: Facsimile: Address: Address: Delivery point: Bornse Weilanden 9 City: Wageningen Administrative area: Gelderland Postal code: 6708 WG Country: NLD Electronic mail address: shokouh.rahsepar@wur.nl Role: Role code: pointOfContact Point of contact: Responsible party: Individual name: Huub Rijnaarts Organisation name: Wageningen University / Environmental Technology Position name: Contact info: Contact: Phone: Telephone: Voice: 0317480743 Facsimile: Address: Address: Delivery point: Bornse Weilanden 9 City: Wageningen Administrative area: Postal code: 6708 WG Country: NLD Electronic mail address: huub.rijnaarts@wur.nl Role: Role code: pointOfContact Descriptive keywords: Keywords: Keyword: aerobic oil biodegradation rate Keyword: bioavailability Keyword: oil weathering Keyword: suspended particles Keyword: EPS formation Type: Keyword type code: theme Descriptive keywords: Keywords: Keyword: inapplicable Type: Keyword type code: place Resource constraints: title: Cite As Constraints: Use limitation: Rahsepar, Shokouh. 2016. Biodegradation of light crude oil using realistic sea water. Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N76W9839 Resource constraints: title: CC0 License Legal constraints: Use constraints: Restriction code: licenceUnrestricted Other constraints: This information is released under the Creative Commons license - No Rights Reserved - CC0 1.0 Universal (https://creativecommons.org/publicdomain/zero/1.0/). The person who associated a work with this deed has dedicated the work to the public domain by waiving all of his or her rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law. You can copy, modify, distribute and perform the work, even for commercial purposes, all without asking permission. Resource constraints: title: Liability and Warranty Legal constraints: Other constraints: All materials on this website are made available to GRIIDC and in turn to you "as-is." Content may only be submitted by an individual who represents and warrants that s/he has sufficient rights to be able to make the content available under a CC0 waiver. There is no warranty (expressed or implied) to these materials, their title, accuracy, non-infringement of third party rights, or fitness for any particular purpose, including the performance or results you may obtain from their use. Use these materials at your own risk. Under no circumstances shall GRIIDC be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use or the inability to use either this website or the materials available via this website. If you are dissatisfied with any website feature, content, or terms of use, your sole and exclusive remedy is to discontinue use. Aggregation Info: AggregateInformation: Aggregate Data Set Name: title: Related Publication Citation Citation: Title: Rahsepar, S., Smit, M. P. J., Murk, A. J., Rijnaarts, H. H. M., & Langenhoff, A. A. M. (2016). Chemical dispersants: Oil biodegradation friend or foe? Marine Pollution Bulletin, 108(1-2), 113–119. doi:10.1016/j.marpolbul.2016.04.044 Date: inapplicable Aggregate Data Set Identifier: title: Related Publication DOI Identifier: Code: Anchor: xlink: https://dx.doi.org/10.1016/j.marpolbul.2016.04.044 title: DOI doi:10.1016/j.marpolbul.2016.04.044 Association Type: Association type code: crossReference Language: eng; USA Topic category: Topic category code: environment Extent: Extent: Description: Laboratory generated data, geographic and temporal extents are not relevant to this dataset. Supplemental Information: There are three files in this dataset; one contains data from experiment conditions 1 through 18, one contains data from experiment conditions 19 through 23, and other file (n-alkane std.xlsx) contains information about the standard concentrations used in calibration. The following is a list of parameters for the dataset file containing conditions 1 through 18: Conditions (1B,S – 18B,S: a more detailed description of each condition can be found in the Data Guide Chart sheet included in this dataset), Sacrifice number (1-10), Duplicates (1, 2), Date (MMDDYYY hh:mm), degradation rate constants (day-1) measured in Total TPH (mg/L), TPH fractions [Peak Area (mV*min)], Individual n-alkanes C11-C40 [Peak Area (mV*min)], benzene [Peak Area (mV*min)], toluene [Peak Area (mV*min)], ethylbenzene [Peak Area (mV*min)], O-M xylene [Peak Area (mV*min)], naphthalene [Peak Area (mV*min)], O2 (%), CO2 (%); Note: Bacterial Taxa conditions 1B to 18B is Rhodococcus qingshengii TUHH-12. The following is a list of parameters for the dataset file containing conditions 19 through 23: Condition No. (19B – 23B), Condition Description [Oil Type, Dispersant to Oil Ratio (DOR), Bacteria (Ps. putida F1, Ps. putida F1 and Rh.TUHH-12), Static Type (Shaken, Static), Msc. (researcher), Elapsed Time (d), O2 consumption (mmol/bottle), Date (Msc., DDMMYYY), Condition Name [Condition No., Sacrifice Number (1-5), Duplicate(1,2)], Benzene [Retention Time (min), Peak Area (mm2), Peak Height (mm)], Toluene [Retention Time (min), Peak Area (mm2), Peak Height (mm)], Ethylbenzene [Retention Time (min), Peak Area (mm2), Peak Height (mm)], O-M Xylene [Retention Time (min), Peak Area (mm2), Peak Height (mm)], P Xylene [Retention Time (min), Peak Area (mm2), Peak Height (mm)], C11-C40 [Retention Time (min), Peak Area (mm2), Peak Height (mm)]; The following is a list of parameters for dataset file n-alkane std.xlsx: Standard ID (Standard A – Standard E), Std. Con. (mg/L), Σ Area [Sum of individual n-alkane peak areas (ΣC)], Individual n-alkanes C11-C40 [Peak Area (mV*min)]; Note: Standard spike volume was 1μL. Additional information about data parameters: The term Static refers to when replicates where standing still during the experimental period and the term Shaken refers to when replicates where located on a shaker during the experimental period to study the possible effect of a dynamic/wave condition on oil biodegradation. |Rhodococcus qingshengii TUHH-12 (DSMZ No. 46766), was used as inoculum in our experiments. The culture was isolated at the Technical University of Hamburg from a seawater sample collected in Spitzbergen, Norway, with an optimal growth temperature of 28C. This culture was maintained in mineral medium with n-hexadecane as the sole carbon source. The medium consisted of 2.6g Na2HPO4, 1.33g KH2PO4, 1g (NH4)2SO4 and 0.20g MgSO4·7 H2O dissolved in 1000mL of demineralized water. The medium was adjusted to pH 7. After sterilization, 5mL of trace element solution and 1mL of vitamin solution were added. The bacterial culture was incubated for three days, and four days prior to the experiments, the culture was transferred into artificial seawater amended with medium salts and n-hexadecane as the carbon source. This resulted in an active culture in its optimal growth phase, as controlled by measuring the Optical Density (OD) with a spectrophotometer (DR3900, Hach Lange) at a wavelength of 600 nm. An OD of 0.98 was taken as a culture in its optimal growth phase. 1 mL of the inoculant was added to each replicate. Pseudomonas putida F1 (Ps. putida F1) was purchased as a freeze dried culture from the German collection of microorganisms and cell cultures (DSMZ, No. 6899). After activation, Ps. putida F1 was transferred to the DSMZ medium No. 457 and supplemented with toluene as a sole carbon source. Four days prior to the experiments, the culture was transferred into seawater amended with medium salts and toluene. This resulted in an active culture in its optimal growth phase, as controlled by measuring the OD. An OD of 0.305 was taken as a culture in its optimal growth phase. 1 mL of the inoculant was added to each replicate. Biodegradation in small batch tests with varying initial conditions.||||N/A
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Distribution info: Distribution: Distributor: Distributor: Distributor contact: Responsible party: Organisation name: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC) Contact info: Contact: Phone: Telephone: Voice: 3618253604 Address: Address: Delivery point: 6300 Ocean Drive City: Corpus Christi Administrative area: TX Postal code: 78412 Country: USA Electronic mail address: griidc@gomri.org Online Resource: Online Resource: Linkage: URL: https://data.gulfresearchinitiative.org Role: Role code: distributor Distributor format: Format: Name: xlsx Version: inapplicable File decompression technique: zip Distributor transfer options: Digital transfer options: Transfer size: 0.9236 Online: Online Resource: Linkage: URL: https://data.gulfresearchinitiative.org/data/R1.x135.119:0002 Protocol: https Name: Data Landing Page Description: GRIIDC dataset landing page Function: Online function code: information
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Metadata maintenance: Maintenance information: Maintenance and update frequency: unknown Maintenance note: This ISO metadata record was automatically generated from information provided to GRIIDC for dataset: R1.x135.119:0002 on 2021-09-23T19:45:48-05:00
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